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1. 发明授权

US08852942B2 Cineraria-derived chromosomal DNA involved in synthesis of flavonoid, and use thereof 有权
标题翻译：涉及黄酮类化合物的花粉衍生染色体DNA及其用途
公开(公告)号：US08852942B2
公开(公告)日：2014-10-07
申请号：US13124985
申请日：2009-10-26
申请人： Yoshikazu Tanaka , Filippa Brugliera
发明人： Yoshikazu Tanaka , Filippa Brugliera
IPC分类号： C12N15/00 , C12N5/04 , C12N5/10 , C12N1/20 , C07H21/04 , C12N9/02 , C12N15/82
CPC分类号： C12N15/825 , C12N9/0073 , C12N15/8225
摘要： Presently provided are a cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H and a F3′5′H gene construct which carries the polynucleotide. The cineraria-derived petal-specific promoter can be as polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 or a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 under stringent conditions and can act as a promoter for a coding region of flavonoid. 3′,5′-hydroxylase (F3′5′H). The gene construct can be used, for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the presently provided cineraria-derived polynucleotide.
摘要翻译：目前提供的是一种能够起到促进黄酮3'，5'-羟化酶（F3'5'H）和携带多核苷酸的F3'5'H基因构建体的编码区的启动子的木质素来源的多核苷酸，衍生花瓣特异性启动子可以是具有SEQ ID NO：9所示核苷酸序列的多核苷酸或可以在严格条件下与具有SEQ ID NO：9所示核苷酸序列的多核苷酸杂交的多核苷酸，并且可以作为 3'，5'-羟化酶（F3'5'H）的编码区，该基因构建体可以用于抑制RNAi方法中的基因的表达，该RNAi方法以环作为包含目前提供的来自牙釉质的多核苷酸的第一内含子的全部或部分。

2. 发明授权

US08629258B2 Plant nucleic acids associated with cellular pH and uses thereof 有权
标题翻译：与细胞pH相关的植物核酸及其用途
公开(公告)号：US08629258B2
公开(公告)日：2014-01-14
申请号：US12303091
申请日：2007-05-28
申请人： Francesca Quattrocchio , Ronald Koes , Walter Verweij , Filippa Brugliera , Kees Spelt , Masako Mizutani
发明人： Francesca Quattrocchio , Ronald Koes , Walter Verweij , Filippa Brugliera , Kees Spelt , Masako Mizutani
IPC分类号： C12N15/29 , C12N15/113 , C12N15/82 , C12N5/10 , A01H5/00 , A01H4/00 , A01H5/02
CPC分类号： C07K14/415 , C12N15/825
摘要： The present invention relates generally to the field of plant molecular biology and agents useful in the manipulation of plant physiological or biochemical properties. More particularly, the present invention provides genetic and proteinaceous agents capable of modulating or altering the level of acidity or alkalinity in a cell, group of cells, organelle, part or reproductive portion of a plant. Genetically altered plants, plant parts, progeny, subsequent generations and reproductive material including flowers or flowering parts having cells exhibiting an altered cellular pH compared to a non-genetically altered plant are also provided.
摘要翻译：本发明一般涉及植物分子生物学领域和用于操作植物生理或生物化学性质的试剂。更具体地，本发明提供能够调节或改变植物的细胞，细胞组，细胞器，部分或生殖部分中的酸度或碱度水平的遗传和蛋白质剂。还提供遗传改变的植物，植物部分，子代，后代和包括花或开花部分的繁殖材料，其具有与非遗传改变的植物相比表现出改变的细胞pH的细胞。

3. 植物专利

USPP23216P2 Dianthus plant named ‘Floriruby’ 有权
标题翻译：石竹植物名为Floriruby
公开(公告)号：USPP23216P2
公开(公告)日：2012-11-27
申请号：US12930278
申请日：2010-12-31
申请人： Filippa Brugliera
发明人： Filippa Brugliera
IPC分类号： A01H5/00
CPC分类号： A01H6/305 , A01H5/02
摘要： A new and distinct cultivar of Dianthus plant named ‘Floriruby’, characterized by its upright plant habit; early and freely flowering habit; spray-type inflorescence form; and purple-colored flowers positioned upright on strong peduncles.
摘要翻译：一个新的和独特的石竹植物名为Floriruby，其特点是其直立的植物习性; 早期自由开花的习惯; 喷雾型花序形式; 紫色花朵直立在坚强的花序梗上。

4. 植物专利

USPP21892P3 Dianthus plant named ‘FLORIAMETRINE’ 有权
标题翻译：石竹植物名为“佛罗里达”
公开(公告)号：USPP21892P3
公开(公告)日：2011-05-03
申请号：US12291865
申请日：2008-11-14
申请人： Filippa Brugliera
发明人： Filippa Brugliera
IPC分类号： A01H5/00
CPC分类号： C12N15/825 , C12N9/0006 , C12N9/0073
摘要： A new cultivar of Dianthus plant named ‘FLORIAMETRINE’ is characterized inter alia by altered inflorescence with respect to tissue and/or organelles including flowers or flower parts. This trait sets ‘FLORIAMETRINE’ apart from all other existing varieties, lines, strains or sports of Dianthus. In particular, Dianthus ‘FLORIAMETRINE’ has bright purple/violet flowers.
摘要翻译：名为“佛罗胺”的新品种石竹植物的特征在于通过改变花序相对于包括花或花朵部分在内的组织和/或细胞器。除了石竹的所有其他现有品种，品系，品种或运动之外，这种特征使“佛罗伦萨”成为可能。特别地，石竹'佛罗力龙'具有明亮的紫色/紫罗兰花。

5. 植物专利

USPP21515P2 Dianthus plant named ‘Florijade’ 有权
标题翻译：石竹植物名为“Florijade”
公开(公告)号：USPP21515P2
公开(公告)日：2010-11-23
申请号：US12454905
申请日：2009-05-26
申请人： Filippa Brugliera
发明人： Filippa Brugliera
IPC分类号： A01H5/00
CPC分类号： A01H5/02
摘要： A new cultivar of Dianthus plant named ‘FLORIJADE’ is characterized, inter alia, by pronounced spray habit, perennial and profuse bloom, and green foliage. Dianthus ‘FLORIJADE’ is suitable for use, for example, as a flowering plant in pots, containers, window boxes, and the garden, but is primarily suited for the production of cut flowers. Dianthus ‘FLORIJADE’ is not hardy and is typically grown in a glasshouse. These traits set ‘FLORIJADE’ apart from all existing varieties, lines, strains, or sports of Dianthus. In particular, Dianthus ‘FLORIJADE’ has bright purple/violet flowers.
摘要翻译：名为“FLORIJADE”的石竹植物新品种的特征是特别是喷雾习性显着，多年生植物和多汁的植物和绿色的叶子。石竹'FLORIJADE'适用于例如花盆，容器，窗框和花园中的开花植物，但主要适用于生产切花。石竹'FLORIJADE'不硬，通常种植在温室中。除了所有现有品种，线条，品种或运动的石竹之外，这些特征都设定了“佛罗伦萨”。特别地，石竹'FLORIJADE'具有明亮的紫色/紫罗兰花。

6. 发明申请

US20070033674A1 Flavanoid 3',5' hydroxylase gene sequences and uses therefor 有权
标题翻译：类黄酮3'，5'羟化酶基因序列及其用途
公开(公告)号：US20070033674A1
公开(公告)日：2007-02-08
申请号：US10526133
申请日：2003-08-29
申请人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
发明人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
IPC分类号： A01H1/00 , C07H21/04 , C12N9/10 , C12N15/82 , C12N5/04
CPC分类号： C12N9/0073 , C12N15/825
摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.
摘要翻译：本发明一般涉及编码具有类黄酮3'，5'-羟化酶（F3'5'H）活性的多肽的遗传序列，以及遗传序列和/或其相应多肽的用途，尤其用于操作颜色花或其部分或其他植物组织。更具体地说，F3'5'H具有调节二氢卡泊芬酚（DHK）代谢以及其它底物如二氢槲皮素（DHQ），柚皮素和二碘代醇的代谢能力。更具体地说，本发明提供编码在玫瑰或非洲菊或植物相关植物中表达时具有F3'5'H活性的多肽的遗传序列。本发明还涉及对应于所有或部分受试者遗传序列或其转录物的反义和有义分子或RNAi诱导分子。本发明还涉及在诸如玫瑰，非洲菊或植物相关植物的植物中有效操作的启动子。

7. 发明申请

US20050081264A1 Genetic sequences encoding flavonoid pathway enzymes and uses therefor 审中-公开
标题翻译：编码黄酮通路酶的遗传序列及其用途
公开(公告)号：US20050081264A1
公开(公告)日：2005-04-14
申请号：US10669962
申请日：2003-09-24
申请人： Filippa Brugliera , Timothy Holton , Michael Michael
发明人： Filippa Brugliera , Timothy Holton , Michael Michael
IPC分类号： A01H5/00 , A01H5/02 , C12N9/02 , C12N15/09 , C12N15/29 , C12N15/63 , C12N15/82 , A01H1/00 , C07H21/04
CPC分类号： C12N9/0071 , C12N15/825
摘要： The present invention relates generally to genetic sequences encoding flavonoid pathway metabolising enzymes and more particularly to flavonoid 3′-hydroxylase (hereinafter referred to as “F3′H”) or derivatives thereof and their use in the manipulation of pigmentation in plants and other organisms.
摘要翻译：本发明一般涉及编码类黄酮途径代谢酶的基因序列，更具体地涉及类黄酮3'-羟化酶（以下称为“F3'H”）或其衍生物，以及它们在植物和其他生物体内操作色素沉着的用途。

8. 发明授权

US06774285B1 Nucleic acid sequences encoding flavonoid 3′-hydroxylase and methods of altering flower color therewith 失效
标题翻译：编码黄酮3'-羟化酶的核酸序列和改变花色的方法
公开(公告)号：US06774285B1
公开(公告)日：2004-08-10
申请号：US09142108
申请日：1999-03-29
申请人： Filippa Brugliera , Timothy Albert Holton , Michael Zenon Michael
发明人： Filippa Brugliera , Timothy Albert Holton , Michael Zenon Michael
IPC分类号： A01H500
CPC分类号： C12N9/0071 , C12N15/825
摘要： The present invention relates generally to nucleic acid sequences encoding flavonoid 3′-hydroxylase (hereinafter referred to as “F3′H”) and their use in the manipulation of pigmentation in flowers of plants.
摘要翻译：本发明一般涉及编码黄酮3'-羟化酶（以下称为“F3'H”）的核酸序列及其在植物花中操作色素沉着的用途。

9. 发明授权

US08445748B2 Flavonoid 3′,5′ hydroxylase gene sequences and uses therefor 有权
公开(公告)号：US08445748B2
公开(公告)日：2013-05-21
申请号：US12566357
申请日：2009-09-24
申请人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
发明人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
IPC分类号： A01H5/00 , C12N15/63 , C12N5/14 , C07H21/00
CPC分类号： C12N9/0073 , C12N15/825
摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

10. 植物专利

USPP23215P2 Dianthus plant named ‘Floriemerald’ 有权
标题翻译：石竹植物名为Floriemerald
公开(公告)号：USPP23215P2
公开(公告)日：2012-11-27
申请号：US12930266
申请日：2010-12-31
申请人： Filippa Brugliera
发明人： Filippa Brugliera
IPC分类号： A01H5/00
CPC分类号： A01H6/305 , A01H5/02
摘要： A new and distinct cultivar of Dianthus plant named ‘Floriemerald’, characterized by its upright plant habit; freely flowering habit; spray-type inflorescence form; and dark purple-colored flowers positioned upright on strong peduncles.
摘要翻译：一个新的和独特的石竹植物名为Floriemerald，其特点是其直立的植物习性; 自由开花的习惯; 喷雾型花序形式; 和深紫色的花朵直立在坚强的花序梗上。

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