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1. 发明授权

US5026640A Process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids 失效
标题翻译：将由微生物产生的类胡萝卜素转化为氰orr烯的方法
公开(公告)号：US5026640A
公开(公告)日：1991-06-25
申请号：US657748
申请日：1984-10-04
申请人： Agnes Kelemen , Eva Cserey Pechany , Istvan Jaksa
发明人： Agnes Kelemen , Eva Cserey Pechany , Istvan Jaksa
IPC分类号： C07D487/22 , C07H23/00 , C12P17/18 , C12P19/42 , C12R1/01 , C12R1/15 , C12R1/225
CPC分类号： C07H23/00 , C12P17/182 , C12P19/42
摘要： The invention relates to a process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids by reaction with cyanides. According to the invention a fermentation broth obtained by disruption of microorganism cells in a known manner, preferably by heat treatment in the presence or absence of sulfite ions, optionally after purification steps known per se, is contacted with a suitable adsorbent or ion exchange resin, the corrinoids adsorbed on the surface of the adsorbent or ion exchange resin are treated with an aqueous solution containing cyanide ions or a compound capable of supplying cyanide ions, in an amount providing cyanide ions in a 1.1 to 2.0-fold molar excess related to the corrinoids, the adsorbent or ion exchange resin is washed with water, and the cyanocorrinoids obtained is eluted in a known manner, preferably with aqueous ethanol.
摘要翻译：本发明涉及通过与氰化物反应将由微生物产生的类胡萝卜素转化成氰orr烯的方法。根据本发明，通过以已知方式破坏微生物细胞获得的发酵液，优选通过在存在或不存在亚硫酸根离子的情况下，任选地在本身已知的纯化步骤之后进行热处理，与合适的吸附剂或离子交换树脂接触，吸附在吸附剂或离子交换树脂表面上的类胡萝卜素用含氰化物离子或能够提供氰化物离子的化合物的水溶液进行处理，其量为氰化物离子的量为1.1-2.0倍摩尔过量，与脯氨酸相关吸附剂或离子交换树脂用水洗涤，所得的氰肟酮以已知的方式，优选用含水乙醇洗脱。

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