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1. 发明授权

US06326192B1 Vector for gene transfer into plant allowing optional deletion of marker gene 失效
标题翻译：用于基因转移到植物中的载体，允许标记基因的任选缺失
公开(公告)号：US06326192B1
公开(公告)日：2001-12-04
申请号：US09147241
申请日：1998-11-09
申请人： Koichi Sugita , Mikiko Uesugi , Etsuko Matsunaga , Hiroyasu Ebinuma
发明人： Koichi Sugita , Mikiko Uesugi , Etsuko Matsunaga , Hiroyasu Ebinuma
IPC分类号： C12N1582
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8209 , C12N15/8213 , C12N15/8242
摘要： The present invention relates to a vector for introducing a desired gene into a plant, wherein a selectable marker gene introduced into a plant cell along with a desired gene is optionally removable from the DNA such as chromosome or the like where it exists and functions, then disappeared the function thereof after its expression, and the expression of the selectable marker gene and the disappearance of the function thereof are detectable by morphological change of the tissue derived from the plant cell into which the selectable marker gene is introduced. Also, the present invention constitutes a vector using a morphological abnormality induction gene as a selectable marker gene, while putting a removable DNA element under control of an inducible promoter, wherein the morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein a desired gene is positioned such that it does not behave integrally with the removable DNA element.
摘要翻译：本发明涉及用于将期望的基因导入植物中的载体，其中与所需基因一起导入植物细胞的选择性标记基因可任选地从其存在并起作用的染色体等DNA脱除，然后其表达后的功能消失，并且可选择标记基因的表达及其功能的消失可以通过导入选择性标记基因的植物细胞衍生的组织的形态学变化来检测。此外，本发明构成使用形态异常诱导基因作为选择性标记基因的载体，同时将可移除的DNA元件置于诱导型启动子的控制下，其中形态异常诱导基因定位成使其与可除去的DNA整体地起作用元件，并且其中将期望的基因定位成使得其不与可移除的DNA元件整体表现。

2. 发明授权

US5965791A Vector for introducing a gene into a plant, and methods for producing transgenic plants and multitudinously introducing genes into a plant using the vector 失效
标题翻译：用于将基因导入植物的载体，以及使用载体生产转基因植物并将基因大量引入植物的方法
公开(公告)号：US5965791A
公开(公告)日：1999-10-12
申请号：US555760
申请日：1995-11-09
申请人： Hiroyasu Ebinuma , Koichi Sugita , Etsuko Matsunaga , Mikiko Yamakado
发明人： Hiroyasu Ebinuma , Koichi Sugita , Etsuko Matsunaga , Mikiko Yamakado
IPC分类号： A01H1/00 , A01H5/00 , C07H21/04 , C12N1/21 , C12N5/00 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/52 , C12N15/82 , C12R1/01 , C12R1/91 , A01H4/00
CPC分类号： C12N15/821 , C12N15/8201 , C12N15/8209 , C12N15/8213 , C12N15/8242
摘要： A vector for introducing a desired gene into a planet, which comprises the desired gene and at least one morphological abnormally induction (MAI) gene as a marker gene, or which comprises the desired gene, at least one MAI gene and a removable element. A method for producing a transgenic plant free from the influence of a marker gene. A method for multitudinously introducing desired genes into one plant.
摘要翻译：用于将期望的基因引入行星的载体，其包含所需基因和至少一种形态异常诱导（MAI）基因作为标记基因，或包含所需基因，至少一种MAI基因和可移除元件。一种不受标记基因影响的转基因植物的制备方法。将所需基因大量引入一种植物的方法。

3. 发明授权

US08927286B2 Method for producing clone seedlings 有权
标题翻译：克隆种苗的生产方法
公开(公告)号：US08927286B2
公开(公告)日：2015-01-06
申请号：US13514402
申请日：2010-12-09
申请人： Kenichi Ogawa , Etsuko Matsunaga , Naoki Negishi , Masatoshi Ohishi , Humiki Kawai , Toshiaki Tanabe , Akiyoshi Kawaoka
发明人： Kenichi Ogawa , Etsuko Matsunaga , Naoki Negishi , Masatoshi Ohishi , Humiki Kawai , Toshiaki Tanabe , Akiyoshi Kawaoka
IPC分类号： C12N5/00 , A01G1/00 , A01G7/00 , A01H4/00
CPC分类号： A01G1/00 , A01G2/00 , A01G7/00 , A01G7/02 , A01G7/045 , A01G22/00 , A01H4/005 , A01N37/44
摘要： A shoot of a plant is cultivated under the presence of glutathione, so that the shoot is rooted. It is possible to cultivate the shoot under the presence of glutathione by use of a rooting medium including glutathione or by contacting a solution including glutathione with the shoot. Oxidized glutathione is preferably used as glutathione. By promoting rooting of the shoot of the plant, a rooting rate of the shoot of the plant is improved. This improves productivity of a clone seedling.
摘要翻译：在谷胱甘肽存在下种植一株植物，使枝条扎根。通过使用包括谷胱甘肽的生根培养基或通过使包含谷胱甘肽的溶液与枝条接触，可以在谷胱甘肽存在下培养芽。氧化的谷胱甘肽优选用作谷胱甘肽。通过促进植物的枝条生根，提高了植株的生根率。这提高了克隆苗的生产力。

4. 发明申请

US20120240462A1 METHOD FOR PRODUCING CLONE SEEDLINGS 有权
标题翻译：生产克隆种子的方法
公开(公告)号：US20120240462A1
公开(公告)日：2012-09-27
申请号：US13514402
申请日：2010-12-09
申请人： Kenichi Ogawa , Etsuko Matsunaga , Naoki Negishi , Masatoshi Ohishi , Humiki Kawai , Toshiaki Tanabe , Akiyoshi Kawaoka
发明人： Kenichi Ogawa , Etsuko Matsunaga , Naoki Negishi , Masatoshi Ohishi , Humiki Kawai , Toshiaki Tanabe , Akiyoshi Kawaoka
IPC分类号： A01N37/44 , A01G1/00 , A01P21/00
CPC分类号： A01G1/00 , A01G2/00 , A01G7/00 , A01G7/02 , A01G7/045 , A01G22/00 , A01H4/005 , A01N37/44
摘要： A shoot of a plant is cultivated under the presence of glutathione, so that the shoot is rooted. It is possible to cultivate the shoot under the presence of glutathione by use of a rooting medium including glutathione or by contacting a solution including glutathione with the shoot. Oxidized glutathione is preferably used as glutathione. By promoting rooting of the shoot of the plant, a rooting rate of the shoot of the plant is improved. This improves productivity of a clone seedling.
摘要翻译：在谷胱甘肽存在下种植一株植物，使枝条扎根。通过使用包括谷胱甘肽的生根培养基或通过使包含谷胱甘肽的溶液与枝条接触，可以在谷胱甘肽存在下培养芽。氧化型谷胱甘肽优选用作谷胱甘肽。通过促进植物的枝条生根，提高了植株的生根率。这提高了克隆苗的生产力。

5. 发明授权

US07294761B2 Method for efficiently producing transgenic plant using auxin precursor 失效
标题翻译：使用生长素前体有效生产转基因植物的方法
公开(公告)号：US07294761B2
公开(公告)日：2007-11-13
申请号：US10626609
申请日：2003-07-25
申请人： Etsuko Matsunaga , Koichi Sugita , Hiroyasu Ebinuma
发明人： Etsuko Matsunaga , Koichi Sugita , Hiroyasu Ebinuma
IPC分类号： C12N15/82 , C12N15/83 , C12N15/84 , C12N15/31 , C12N15/54 , C12N15/55
CPC分类号： C12N15/821
摘要： A method for producing a transgenic plant, which comprises: (A) introducing a vector into a plant cell, wherein the vector is a vector for gene introduction into a plant and comprises: a desired gene, and a selectable marker gene comprising a gene encoding an enzyme which synthesizes auxin from an auxin precursor; (B) culturing the plant cell into which the genes are introduced by the vector, in the presence of an auxin precursor and/or an analogue thereof to thereby prepare a redifferentiated tissue, and detecting and selecting the redifferentiated tissues; and (C) culturing the redifferentiated tissue selected in (B) to redifferentiate a plant individual, and a vector for gene introduction into a plant, which comprises: a desired gene, and a selectable marker gene comprising an indoleacetamide hydrolase, iaaH, gene and an isopentenyl transferase, ipt, gene and being free of an tryptophan monooxygenase, iaaM, gene.
摘要翻译：一种生产转基因植物的方法，其包括：（A）将载体导入植物细胞，其中所述载体是用于将基因导入植物的载体，并且包含：所需基因和选择标记基因，所述选择标记基因包含编码从生长素前体合成生长素的酶; （B）在生长素前体和/或其类似物的存在下培养载体引入基因的植物细胞，由此制备再分化组织，并检测并选择再分化组织; （C）培养在（B）中选择的再分化组织以重新分化植物个体和将基因导入植物的载体，其包含：所需基因和包含吲哚乙酰胺水解酶，iaaH基因和异戊烯基转移酶，ipt，基因，并且不含色氨酸单加氧酶，iaaM基因。

6. 发明授权

US06294714B1 Method for introducing a gene into a plant using an adventitious bud redifferentiation gene under the control of a light-inducible promoter as a selectable marker gene, and vector for introducing a gene into a plant using the same 失效
标题翻译：使用在光诱导型启动子作为选择性标记基因的控制下的不定芽再分化基因将基因引入植物的方法，以及使用其将基因导入植物的载体
公开(公告)号：US06294714B1
公开(公告)日：2001-09-25
申请号：US09354305
申请日：1999-07-16
申请人： Etsuko Matsunaga , Takehide Kasahara , Koichi Sugita , Hiroyasu Ebinuma
发明人： Etsuko Matsunaga , Takehide Kasahara , Koichi Sugita , Hiroyasu Ebinuma
IPC分类号： C12N504
CPC分类号： A01H4/005 , C12N15/8205 , C12N15/8209
摘要： A method for introducing a gene into a plant, which comprises introducing a gene into a plant cell using a vector containing an adventitious shoot redifferentiation gene as a selectable marker gene under the control of a light-inducible promoter, and a vector for introducing a gene into a plant, which comprises a desired gene, an adventitious shoot redifferentiation gene as a selectable marker gene under the control of a light-inducible promoter, and a removable DNA element, wherein the selectable marker gene is positioned such that it behaves integrally with the removable DNA element, and wherein the desired gene is positioned such that it does not behave integrally with the removable DNA element.
摘要翻译：将基因导入植物的方法，其特征在于，在光诱导型启动子的控制下，使用含有不定芽再分化基因的载体作为选择性标记基因，将基因引入植物细胞，以及用于引入基因的载体包含所需基因的植物，作为在光诱导型启动子控制下的选择性标记基因的不定芽再分化基因和可除去的DNA元件，其中所述选择标记基因定位成使其与可去除的DNA元件，并且其中所需基因定位成使其不与可移除的DNA元件整体地表现。

7. 发明授权

US5654190A Process for producing plant belonging to the section leuce 失效
标题翻译：生产属于断面的植物的过程
公开(公告)号：US5654190A
公开(公告)日：1997-08-05
申请号：US443161
申请日：1995-05-17
申请人： Etsuko Matsunaga , Ayako Todate , Hiroyasu Ebinuma
发明人： Etsuko Matsunaga , Ayako Todate , Hiroyasu Ebinuma
IPC分类号： A01H4/00 , C12N5/04 , C12N5/00
CPC分类号： C12N5/04 , A01H4/001
摘要： A process for producing a plant is described, which comprises the step of culturing a tissue of a plant belonging to the genus Populus, the section Leuce, thereby regenerating the plant via an adventitious bud, wherein the adventitious bud is differentiated from the cultured tissue using an adventitious bud differentiation medium having nitrogen source concentrations of from 1 to 15 mM as ammonia-nitrogen and from 15 to 50 mM as nitrate-nitrogen.
摘要翻译：描述了一种用于生产植物的方法，其包括培育属于杨属的植物的组织，Leuce部分的步骤，从而通过不定芽再生植物，其中不定芽与培养的组织分化，使用具有氮源浓度为1至15mM的氮源和15至50mM作为硝酸盐 - 氮的不定芽分化培养基。

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