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1. 发明申请

US20090104628A1 Method for quantifying phosphokinase activity on proteins 有权
标题翻译：蛋白质磷酸激酶活性定量的方法
公开(公告)号：US20090104628A1
公开(公告)日：2009-04-23
申请号：US12179526
申请日：2008-07-24
申请人： Kevin J. REAGAN , Erik Schaefer , Jimin Wang
发明人： Kevin J. REAGAN , Erik Schaefer , Jimin Wang
IPC分类号： G01N33/573 , C07K16/40
CPC分类号： C07K16/2863 , C07K16/18 , C07K2317/34 , C12Q1/485 , G01N2333/4709
摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.
摘要翻译：本发明涉及一种用于测量特定位点蛋白质磷酸化的方法，因此是能够使这些位点磷酸化的酶的蛋白激酶活性的指标。该方法包括在特定丝氨酸，苏氨酸或酪氨酸残基处的靶蛋白的体外或体内磷酸化，使该蛋白质（非磷酸化）到含有所有试剂的反应混合物，包括磷酸激酶，其允许产生磷酸化形式蛋白。通过使磷酸化蛋白质与针对磷酸化位点特异的抗体接触来测量磷酸化蛋白质。本发明包括可用于实践本发明方法的抗体。本发明特别涉及通过磷酸化和去磷酸化修饰的所有蛋白质，如Tau，Rb和EGFR蛋白质所示，以及对Tau，Rb或EGFR蛋白质的磷酸化位点特异的抗体。

2. 发明申请

US20120135426A1 Method for quantifying phosphokinase activity on proteins 审中-公开
公开(公告)号：US20120135426A1
公开(公告)日：2012-05-31
申请号：US13245045
申请日：2011-09-26
申请人： Kevin J. Reagan , Erik Schaefer , Jimin Wang
发明人： Kevin J. Reagan , Erik Schaefer , Jimin Wang
IPC分类号： G01N33/566 , C07K16/18
CPC分类号： C07K16/2863 , C07K16/18 , C07K2317/34 , C12Q1/485 , G01N2333/4709
摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.

3. 发明授权

US08114617B2 Method for quantifying phosphokinase activity on proteins 有权
标题翻译：蛋白质磷酸激酶活性定量的方法
公开(公告)号：US08114617B2
公开(公告)日：2012-02-14
申请号：US12970250
申请日：2010-12-16
申请人： Kevin J. Reagan , Erik Schaefer , Jimin Wang
发明人： Kevin J. Reagan , Erik Schaefer , Jimin Wang
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： C07K16/2863 , C07K16/18 , C07K2317/34 , C12Q1/485 , G01N2333/4709
摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.
摘要翻译：本发明涉及一种用于测量特定位点蛋白质磷酸化的方法，因此是能够使这些位点磷酸化的酶的蛋白激酶活性的指标。该方法包括在特定丝氨酸，苏氨酸或酪氨酸残基处的靶蛋白的体外或体内磷酸化，使该蛋白质（非磷酸化）到含有所有试剂的反应混合物，包括磷酸激酶，其允许产生磷酸化形式蛋白。通过使磷酸化蛋白质与针对磷酸化位点特异性的抗体接触来测量磷酸化蛋白质。本发明包括可用于实践本发明方法的抗体。本发明特别涉及通过磷酸化和去磷酸化修饰的所有蛋白质，如Tau，Rb和EGFR蛋白质所示，以及对Tau，Rb或EGFR蛋白质的磷酸化位点特异的抗体。

4. 发明申请

US20110165588A1 METHOD FOR QUANTIFYING PHOSPHOKINASE ACTIVITY ON PROTEINS 有权
标题翻译：蛋白质磷酸酶活性定量的方法
公开(公告)号：US20110165588A1
公开(公告)日：2011-07-07
申请号：US12970250
申请日：2010-12-16
申请人： Kevin J. Reagan , Erik Schaefer , Jimin Wang
发明人： Kevin J. Reagan , Erik Schaefer , Jimin Wang
IPC分类号： G01N33/53
CPC分类号： C07K16/2863 , C07K16/18 , C07K2317/34 , C12Q1/485 , G01N2333/4709
摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.
摘要翻译：本发明涉及一种用于测量特定位点蛋白质磷酸化的方法，因此是能够使这些位点磷酸化的酶的蛋白激酶活性的指标。该方法包括在特定丝氨酸，苏氨酸或酪氨酸残基处的靶蛋白的体外或体内磷酸化，使该蛋白质（非磷酸化）到含有所有试剂的反应混合物，包括磷酸激酶，其允许产生磷酸化形式蛋白。通过使磷酸化蛋白质与针对磷酸化位点特异性的抗体接触来测量磷酸化蛋白质。本发明包括可用于实践本发明方法的抗体。本发明特别涉及通过磷酸化和去磷酸化修饰的所有蛋白质，如Tau，Rb和EGFR蛋白质所示，以及对Tau，Rb或EGFR蛋白质的磷酸化位点特异的抗体。

5. 发明授权

US07888050B2 Method for quantifying phosphokinase activity on proteins 有权
标题翻译：蛋白质磷酸激酶活性定量的方法
公开(公告)号：US07888050B2
公开(公告)日：2011-02-15
申请号：US12179526
申请日：2008-07-24
申请人： Kevin Reagan , Erik Schaefer , Jimin Wang
发明人： Kevin Reagan , Erik Schaefer , Jimin Wang
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： C07K16/2863 , C07K16/18 , C07K2317/34 , C12Q1/485 , G01N2333/4709
摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.
摘要翻译：本发明涉及一种用于测量特定位点蛋白质磷酸化的方法，因此是能够使这些位点磷酸化的酶的蛋白激酶活性的指标。该方法包括在特定丝氨酸，苏氨酸或酪氨酸残基处的靶蛋白的体外或体内磷酸化，使该蛋白质（非磷酸化）到含有所有试剂的反应混合物，包括磷酸激酶，其允许产生磷酸化形式蛋白。通过使磷酸化蛋白质与针对磷酸化位点特异性的抗体接触来测量磷酸化蛋白质。本发明包括可用于实践本发明方法的抗体。本发明特别涉及通过磷酸化和去磷酸化修饰的所有蛋白质，如Tau，Rb和EGFR蛋白质所示，以及对Tau，Rb或EGFR蛋白质的磷酸化位点特异的抗体。

6. 发明申请

US20070196860A1 Methods for Measuring Real Time Kinase Activity 审中-公开
标题翻译：测量实时激酶活性的方法
公开(公告)号：US20070196860A1
公开(公告)日：2007-08-23
申请号：US11624686
申请日：2007-01-18
申请人： Kyle Gee , Min Li , Xiao-Dong Qian , Erik Schaefer
发明人： Kyle Gee , Min Li , Xiao-Dong Qian , Erik Schaefer
IPC分类号： G01N33/53 , C07K16/46
CPC分类号： C12Q1/485 , G01N33/573
摘要： The present invention relates to methods for detecting and/or measuring the activity of a specific kinase, with the methods comprising contacting one or more kinases with a binding agent to isolate a specific kinase of interest. The isolated kinase is then contacted with a kinase activity sensor, where the kinase activity sensor is comprised of a kinase recognition motif that is capable of being recognized by the isolated kinase, and at least one phosphorylation site. The isolated kinase phosphorylates the amino acid target of the kinase activity sensor and levels of the phosphorylated target amino acid can then be quantified.
摘要翻译：本发明涉及用于检测和/或测量特定激酶活性的方法，其中所述方法包括使一种或多种激酶与结合剂接触以分离特定的目标激酶。然后将分离的激酶与激酶活性传感器接触，其中激酶活性传感器由能够被分离的激酶识别的激酶识别基序和至少一个磷酸化位点组成。分离的激酶磷酸化激酶活性传感器的氨基酸靶标，然后可以量化磷酸化靶氨基酸的水平。

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