专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20090017459A1 MEASURING ANALYTES USING COMPETITIVE INTERFERENCE AND RECUPERATION OF ENZYME ACTIVITY 有权
标题翻译：使用竞争性干扰和酶活性的测定来测量分析
公开(公告)号：US20090017459A1
公开(公告)日：2009-01-15
申请号：US12126827
申请日：2008-05-23
申请人： DONGYUAN XIA
发明人： DONGYUAN XIA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , G01N2458/10 , C12Q2537/161
摘要： The present teaching generally discloses a method of measuring the concentration of an analyte in a solution. The method includes selecting a binding compound; selecting an analog to an analyte and conjugating the analog to a polynucleotide substrate to construct a reagent for measuring the amount of the analyte in an assay solution using a desired polynucleotide reaction that comprises a polymerization reaction, a cleavage reaction, or a recombination reaction. The binding of the reagent to the binding compound in the assay solution competes with the binding of the analyte to the binding compound in the assay solution. The method also includes selecting a desired enzyme for catalyzing the desired polynucleotide reaction. Reagents and kits are also provided.
摘要翻译：本教导通常公开了一种测量溶液中分析物浓度的方法。该方法包括选择结合化合物; 选择与分析物的类似物并将所述类似物与多核苷酸底物缀合，以构建用于使用包含聚合反应，裂解反应或重组反应的所需多核苷酸反应来测量测定溶液中分析物的量的试剂。在测定溶液中试剂与结合化合物的结合与测定溶液中分析物与结合化合物的结合相竞争。该方法还包括选择所需的酶以催化所需的多核苷酸反应。还提供试剂和试剂盒。

2. 发明授权

US07888027B2 Measuring analytes using competitive interference and recuperation of enzyme activity 有权
标题翻译：使用竞争性干扰测定分析物并恢复酶活性
公开(公告)号：US07888027B2
公开(公告)日：2011-02-15
申请号：US12126827
申请日：2008-05-23
申请人： Dongyuan Xia
发明人： Dongyuan Xia
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , G01N2458/10 , C12Q2537/161
摘要： The present teaching generally discloses a method of measuring the concentration of an analyte in a solution. The method includes selecting a binding compound; selecting an analog to an analyte and conjugating the analog to a polynucleotide substrate to construct a reagent for measuring the amount of the analyte in an assay solution using a desired polynucleotide reaction that comprises a polymerization reaction, a cleavage reaction, or a recombination reaction. The binding of the reagent to the binding compound in the assay solution competes with the binding of the analyte to the binding compound in the assay solution. The method also includes selecting a desired enzyme for catalyzing the desired polynucleotide reaction. Reagents and kits are also provided.
摘要翻译：本教导通常公开了一种测量溶液中分析物浓度的方法。该方法包括选择结合化合物; 选择与分析物的类似物并将所述类似物与多核苷酸底物缀合，以构建用于使用包含聚合反应，裂解反应或重组反应的所需多核苷酸反应来测量测定溶液中分析物的量的试剂。在测定溶液中试剂与结合化合物的结合与测定溶液中分析物与结合化合物的结合相竞争。该方法还包括选择所需的酶以催化所需的多核苷酸反应。还提供试剂和试剂盒。

3. 发明授权

US06936470B2 Rapid and enzymeless cloning of nucleic acid fragments 失效
标题翻译：快速和无酶克隆的核酸片段
公开(公告)号：US06936470B2
公开(公告)日：2005-08-30
申请号：US10125789
申请日：2002-04-16
申请人： Xiaowu Liang , Andy Teng , Shizhong Chen , Dongyuan Xia , Philip L. Felgner
发明人： Xiaowu Liang , Andy Teng , Shizhong Chen , Dongyuan Xia , Philip L. Felgner
IPC分类号： C12N15/00 , C12N15/10 , C12N15/66 , C12N15/85 , C12P19/34 , C12N15/63 , C12N15/09 , C12N15/87
CPC分类号： C12N15/66 , C07K2319/21 , C07K2319/23 , C07K2319/42 , C07K2319/43 , C07K2319/60 , C12N15/10
摘要： A method for cloning a nucleic acid fragment into a vector by flanking the fragment with first and second adapter sequences, and contacting the fragment with the vector having sequences homologous to the first and second adapter sequences under conditions such that the nucleic acid fragment is incorporated into the vector by homologous recombination in vivo in a host cell. Additionally, a method for selecting for a successful transformation of a vector by a nucleic acid insert. Also, systems for cloning a nucleic acid fragment into a vector without at least one of a restriction enzyme, a ligase, a gyrase, a single stranded DNA binding protein, or other DNA modifying enzymes. Further, a kit for cloning a nucleic acid fragment into a vector.
摘要翻译：一种通过用第一和第二衔接子序列侧接该片段将核酸片段克隆到载体中的方法，并使该片段与具有与第一和第二衔接子序列同源的序列的载体接触，使得将该核酸片段并入载体通过宿主细胞中的体内同源重组。另外，用于通过核酸插入物成功转化载体的方法。而且，用于将核酸片段克隆到载体中而不具有限制酶，连接酶，促旋酶，单链DNA结合蛋白或其它DNA修饰酶中的至少一种的系统。另外，用于将核酸片段克隆入载体的试剂盒。

4. 发明申请

US20050233422A1 Rapid and enzymeless cloning of nucleic acid fragments 审中-公开
公开(公告)号：US20050233422A1
公开(公告)日：2005-10-20
申请号：US11045686
申请日：2005-01-28
申请人： Xiaowu Liang , Andy Teng , Shizhong Chen , Dongyuan Xia , Philip Felgner
发明人： Xiaowu Liang , Andy Teng , Shizhong Chen , Dongyuan Xia , Philip Felgner
IPC分类号： C12N15/00 , C12N15/10 , C12N15/66 , C12N15/85 , C12P19/34
CPC分类号： C12N15/66 , C07K2319/21 , C07K2319/23 , C07K2319/42 , C07K2319/43 , C07K2319/60 , C12N15/10
摘要： A method for cloning a nucleic acid fragment into a vector by flanking the fragment with first and second adapter sequences, and contacting the fragment with the vector having sequences homologous to the first and second adapter sequences under conditions such that the nucleic acid fragment is incorporated into the vector by homologous recombination in vivo in a host cell. Additionally, a method for selecting for a successful transformation of a vector by a nucleic acid insert. Also, systems for cloning a nucleic acid fragment into a vector without at least one of a restriction enzyme, a ligase, a gyrase, a single stranded DNA binding protein, or other DNA modifying enzymes. Further, a kit for cloning a nucleic acid fragment into a vector.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式