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1. 发明申请

US20110186434A1 QUALITATIVE AND/OR QUANTITATIVE DETERMINATION OF A PROTEINACEOUS MOLECULE IN A PLURALITY OF SAMPLES 审中-公开
标题翻译：蛋白质分子在多种样品中的定性和/或定量测定
公开(公告)号：US20110186434A1
公开(公告)日：2011-08-04
申请号：US13055384
申请日：2009-07-23
申请人： Kai Naumann , Dierk Scheel
发明人： Kai Naumann , Dierk Scheel
IPC分类号： G01N27/447 , G01N33/53
CPC分类号： G01N27/44721
摘要： The present invention relates to the qualitative and/or quantitative determination of a proteinaceous molecule in a plurality of samples by combining a particular labeling and fractionation strategy. In particular, each sample is provided with a dye chosen from a set of dyes, wherein each dye within the set of dyes emits luminescent light at a wavelength that is sufficiently different from the emitted luminescent light of the remaining dyes in the set of dyes to provide a different light signal when excited. Subsequent to labelling the proteinaceous molecule with the fluorescent dye, the samples are combined and fractionated by means of electrophoresis, the labeled proteinaceous molecule having a relative electrophoretic mobility that differs from the electrophoretic mobility of the proteinaceous molecule labeled with another dye within the set of dyes. After capturing separate images of the labeled proteinaceous molecule at different wavelengths, the images are aligned by means of image processing. The present invention further relates to a kit and a set of luminescent dyes for use in qualitatively and/or quantitatively determining a proteinaceous molecule in a plurality of samples by means of electrophoresis.
摘要翻译：本发明涉及通过组合特定标记和分馏策略来定性和/或定量测定多个样品中的蛋白质分子。特别地，每个样品都提供有选自一组染料的染料，其中该组染料中的每种染料以与该组染料中剩余染料的发射的发光不同的波长发出发光，当激发时提供不同的光信号。在用荧光染料标记蛋白质分子之后，将样品合并并通过电泳分级分离，标记的蛋白质分子具有相对电泳迁移率，其不同于在该组染料内用另一种染料标记的蛋白质分子的电泳迁移率。在捕获不同波长的标记蛋白质分子的单独图像之后，通过图像处理对图像进行对准。本发明还涉及用于通过电泳定性和/或定量测定多个样品中的蛋白质分子的试剂盒和一组发光染料。

2. 发明申请

US20070011772A1 Polynucleotides encoding a beta-glucosidase and uses thereof 审中-公开
标题翻译：编码β-葡糖苷酶的多核苷酸及其用途
公开(公告)号：US20070011772A1
公开(公告)日：2007-01-11
申请号：US10506670
申请日：2003-03-06
申请人： Volker Lipka , Dierk Scheel , Paul Schulze-Lefert , Sabine Rosahl , Jorn Landtag
发明人： Volker Lipka , Dierk Scheel , Paul Schulze-Lefert , Sabine Rosahl , Jorn Landtag
IPC分类号： A01H5/00 , C12Q1/68 , G06F19/00 , C07H21/04 , C12N15/82 , A01H1/00 , C12N5/04 , C07K14/415 , C12N9/48
CPC分类号： C12Y302/01147 , C12N9/2445 , C12N15/8282 , C12Y302/01021
摘要： Disclosed are polynucleotides that encode a beta-glucosidase belonging to the plant protein class of family 1 glycoside hydrolases. Furthermore described are recombinant nucleic molecules and vectors containing these polynucleotides and host cells, preferably plant cells that are genetically engineered with these polynucleotides. In addition, transgenic plants are disclosed which show an increased activity of the beta-glucosidase described, preferably leading to an improved resistance against plant pathogens. In another aspect, the present application discloses screening methods for identifying a substrate for said betaglucosidase and methods for the production of plant protection compositions from such substrates or hydrolysis products thereof. Furthermore, methods and uses are disclosed which apply the polynucleotides encoding the beta-glucosidase described in order to improve resistance in plants against plant pathogens.
摘要翻译：公开了编码属于1型糖苷水解酶的植物蛋白质类型的β-葡糖苷酶的多核苷酸。此外还描述了含有这些多核苷酸和宿主细胞的重组核酸分子和载体，优选用这些多核苷酸进行遗传工程改造的植物细胞。此外，公开了显示所描述的β-葡糖苷酶活性增加的转基因植物，优选导致对植物病原体的改善的抗性。另一方面，本申请公开了用于鉴定所述β-葡糖苷酶的底物的筛选方法和用于从这种底物或其水解产物生产植物保护组合物的方法。此外，公开了应用编码所述β-葡糖苷酶的多核苷酸以提高植物对植物病原体抗性的方法和用途。

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