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1. 发明申请

US20060024756A1 Methods and algorithms for cell enumeration in low-cost cytometer 有权
标题翻译：低成本细胞计数器细胞计数的方法和算法
公开(公告)号：US20060024756A1
公开(公告)日：2006-02-02
申请号：US10903798
申请日：2004-07-30
申请人： Arjan Tibbe , Jan Greve , Dhanesh Gohel , Erik Droog , Leon Terstappen
发明人： Arjan Tibbe , Jan Greve , Dhanesh Gohel , Erik Droog , Leon Terstappen
IPC分类号： G01N33/53
CPC分类号： G01N15/1475 , G01N15/1463 , G01N33/5094 , G01N33/54333 , G01N2015/008 , G01N2015/1486 , Y10T436/101666 , Y10T436/25375
摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.
摘要翻译：通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

2. 发明授权

US08128890B2 Methods and algorithms for cell enumeration in a low-cost cytometer 有权
标题翻译：低成本细胞计数器细胞计数的方法和算法
公开(公告)号：US08128890B2
公开(公告)日：2012-03-06
申请号：US12917000
申请日：2010-11-01
申请人： Erik Droog , Dhanesh Gohel , Arjan G. J. Tibbe , Jan Greve , Leon W. M. M. Terstappen
发明人： Erik Droog , Dhanesh Gohel , Arjan G. J. Tibbe , Jan Greve , Leon W. M. M. Terstappen
IPC分类号： G01N33/52 , G01N33/553
CPC分类号： G01N15/1475 , G01N15/1463 , G01N33/5094 , G01N33/54333 , G01N2015/008 , G01N2015/1486 , Y10T436/101666 , Y10T436/25375
摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.
摘要翻译：通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

3. 发明申请

US20110044527A1 Methods and Algorithms for Cell Enumeration in a Low-Cost Cytometer 有权
标题翻译：低成本细胞计数器细胞计数的方法和算法
公开(公告)号：US20110044527A1
公开(公告)日：2011-02-24
申请号：US12917000
申请日：2010-11-01
申请人： Arjan Tibbe , Jan Greve , Dhanesh Gohel , Erik Droog , Leon W.M.M. Terstappen
发明人： Arjan Tibbe , Jan Greve , Dhanesh Gohel , Erik Droog , Leon W.M.M. Terstappen
IPC分类号： G06K9/00
CPC分类号： G01N15/1475 , G01N15/1463 , G01N33/5094 , G01N33/54333 , G01N2015/008 , G01N2015/1486 , Y10T436/101666 , Y10T436/25375
摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.
摘要翻译：通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

4. 发明授权

US07943397B2 Methods and algorithms for cell enumeration in low-cost cytometer 有权
标题翻译：低成本细胞计数器细胞计数的方法和算法
公开(公告)号：US07943397B2
公开(公告)日：2011-05-17
申请号：US10903798
申请日：2004-07-30
申请人： Arjan Tibbe , Jan Greve , Dhanesh Gohel , Erik Droog , Leon W. M. M. Terstappen
发明人： Arjan Tibbe , Jan Greve , Dhanesh Gohel , Erik Droog , Leon W. M. M. Terstappen
IPC分类号： G01N33/00 , G01N33/553
CPC分类号： G01N15/1475 , G01N15/1463 , G01N33/5094 , G01N33/54333 , G01N2015/008 , G01N2015/1486 , Y10T436/101666 , Y10T436/25375
摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.
摘要翻译：通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

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