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1. 发明授权

US07195872B2 High surface area substrates for microarrays and methods to make same 有权
标题翻译：用于微阵列的高表面积底物和使其形成的方法
公开(公告)号：US07195872B2
公开(公告)日：2007-03-27
申请号：US10291467
申请日：2002-11-08
申请人： Anoop Agrawal , John P. Cronin , Juan C. Tonazzi , A. G. Goodyear , Robert S. LeCompte , Michael E. Hogan , David W. Galbraith
发明人： Anoop Agrawal , John P. Cronin , Juan C. Tonazzi , A. G. Goodyear , Robert S. LeCompte , Michael E. Hogan , David W. Galbraith
IPC分类号： C12Q1/68
CPC分类号： B81C1/00206 , B01J19/0046 , B01J2219/00317 , B01J2219/00378 , B01J2219/00387 , B01J2219/00504 , B01J2219/00527 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00628 , B01J2219/00635 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00734 , B01J2219/00743 , B01L3/5085 , B01L3/5088 , B01L2200/12 , B01L2300/0819 , B01L2300/16 , B01L2300/166 , B81B1/006 , B81B2201/0214
摘要： The present invention is directed to a substrate having a plurality of microfeatures that provide a high surface area and are open to provide ready access to fluids and components therein. Methods of making the high surface area substrates are described and include generating microfeatures and/or microstructures on the surface of the substrate.
摘要翻译：本发明涉及一种具有多个微特征的基底，该微特征提供高的表面积并且是开放的以提供对其中的流体和组分的准备进入。描述了制造高表面积基板的方法，包括在基板的表面上产生微特征和/或微结构。

2. 发明授权

US4300310A Identification and sorting of plant heterokaryons 失效
标题翻译：鉴定和分选植物异核子
公开(公告)号：US4300310A
公开(公告)日：1981-11-17
申请号：US172979
申请日：1980-07-28
申请人： David W. Galbraith
发明人： David W. Galbraith
IPC分类号： A01H1/02 , C12N5/14
CPC分类号： C12N5/14 , A01H1/02
摘要： To label heterokaryons formed by fusion of different somatic tissue cells, somatic tissue cells from one plant are immersed in a first solution including polysaccharidase and fluorescein isothiocyanate and somatic tissue cells from a different plant are immersed in a second solution including polysaccharidase and rhodamine isothiocyanate for a period of from 12 to 18 hours, after which protoplasts emerge, with those from one plant fluorescing green-yellow and those from the other fluorescing orange-red. The protoplasts are then fused in a polyethylene glycol solution. The heterokaryons are identified and sorted by a commercial cell sorter which relies upon the fluorescence from both markers.
摘要翻译：为了标记由不同体细胞组织细胞融合形成的异核体，将来自一个植物的体细胞组织细胞浸入包括多糖酶和异硫氰酸荧光素的第一溶液中，并将来自不同植物的体细胞组织细胞浸入含有多糖酶和罗丹明异硫氰酸酯的第二溶液中时间为12至18小时，之后出现原生质体，其中来自一个植物荧光为绿黄色，另一个荧光为橙红色。然后将原生质体融合在聚乙二醇溶液中。通过商业细胞分选器鉴定和分选异源核苷酸，其依赖于两种标记物的荧光。

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