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    • 8. 发明申请
    • INDUCED PLURIPOTENT STEM CELLS
    • 诱导的大脑干细胞
    • US20120128655A1
    • 2012-05-24
    • US13260897
    • 2010-04-01
    • Dohoon KimChun-Hyung KimKwang-Soo Kim
    • Dohoon KimChun-Hyung KimKwang-Soo Kim
    • C12N5/071A61K8/98
    • C12N5/0696C12N2501/602C12N2501/603C12N2501/604C12N2501/606
    • The present invention concerns the delivery of certain reprogramming factor proteins into cells, such as differenti-atedsomatic cells, in order to induce the epi-genetic reprogramming of the cell so it becomes a pluripotent stem cell. The reprogramming factor protein(s) may be Sox2, Klf4, Oct3/4, c-Myc, Lin28, Nanog, or any protein with reprogramming (-enhancing) activity. These proteins may be linked recombinantly or chemically to a cell penetrating peptide that helps facilitate the introduction of these proteins into the target cell and may be preferably expressed in mammalian cells to maintain them in active forms. Accordingly, the present method of inducing pluripotent stem cell (iPS) formation avoids the use of viral or DNA-based expression vectors or the expression of reprogramming factor genes within target cells, which are known to be harmful to the host target cell and cause cancer.
    • 本发明涉及将某些重编程因子蛋白递送到细胞中,例如分化细胞,以便诱导细胞的外显重编程,使其成为多能干细胞。 重编程因子蛋白可以是Sox2,Klf4,Oct3 / 4,c-Myc,Lin28,Nanog或具有重编程(增强)活性的任何蛋白质。 这些蛋白质可以重组或化学地连接到细胞穿透肽,这有助于促进将这些蛋白质引入靶细胞,并且可优选在哺乳动物细胞中表达以维持它们为活性形式。 因此,本发明的诱导多能干细胞(iPS)形成的方法避免了使用基于病毒或DNA的表达载体或靶细胞中重编程因子基因的表达,其已知对宿主靶细胞有害并引起癌症 。