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1. 发明申请

US20130330758A1 METHOD FOR USING AN ENZYME RESISTANT TO HIGH PRESSURES 有权
标题翻译：使用抗高温抗菌的方法
公开(公告)号：US20130330758A1
公开(公告)日：2013-12-12
申请号：US14000497
申请日：2012-02-20
申请人： Nam-Soo Kim , Chong-Tai Kim , Yong-Jin Cho , Chul-Jin Kim , Jin-Soo Maeng , Soo-Jin Kwon
发明人： Nam-Soo Kim , Chong-Tai Kim , Yong-Jin Cho , Chul-Jin Kim , Jin-Soo Maeng , Soo-Jin Kwon
IPC分类号： C12N9/64 , C12N9/48 , C12N9/52 , C12N9/76
CPC分类号： C12N9/6481 , A23L3/0155 , A23L5/17 , A23L27/00 , A23L27/21 , A23L29/06 , A61K38/48 , A61K38/51 , C12N9/48 , C12N9/485 , C12N9/52 , C12N9/6427 , C12Q1/37 , C12Y304/00
摘要： At least at least one embodiment of the present invention relates to a method for using a high pressure-resistant enzyme in a high pressure condition; a method for promoting the activity of the high pressure-resistant enzyme by means of a high pressure treatment; a composition, which contains the high pressure-resistant enzyme, for decomposing proteins under a high pressure condition; a composition, which contains the composition for decomposing proteins, for preparing natural flavoring substances; a container for high pressure treatment, which contains the composition for decomposing proteins; and a method for measuring the activity of the high pressure-resistant enzyme, which comprises a step of decomposing an azocasein solution serving as a substrate by using the high pressure-resistant enzyme treated under a high pressure condition.
摘要翻译：本发明的至少一个实施方案涉及在高压条件下使用高耐压酶的方法; 通过高压处理促进高耐压酶的活性的方法; 含有高耐压酶的组合物，用于在高压条件下分解蛋白质; 含有用于分解蛋白质的组合物的组合物，用于制备天然调味物质; 用于高压处理的容器，其包含用于分解蛋白质的组合物; 以及测定高耐压酶的活性的方法，其包括通过使用在高压条件下处理的高耐压酶分解作为底物的偶氮酪蛋白溶液的步骤。

2. 发明授权

US10036073B2 Slide chip for detection sensor of food-borne pathogens and preparation method thereof 有权
公开(公告)号：US10036073B2
公开(公告)日：2018-07-31
申请号：US14343213
申请日：2012-02-22
申请人： Young Jin Cho , Chul Jin Kim , Nam Soo Kim , Chong Tai Kim , Jin Soo Maeng , Tae Eun Kim , Myung Hyun Lee
发明人： Young Jin Cho , Chul Jin Kim , Nam Soo Kim , Chong Tai Kim , Jin Soo Maeng , Tae Eun Kim , Myung Hyun Lee
IPC分类号： C12Q1/68 , C12Q1/689 , C12Q1/04 , G01N33/543 , G01N33/553 , G01N33/569 , G01N33/50 , C12N15/11
CPC分类号： C12Q1/689 , C12N15/111 , C12N2310/16 , C12N2320/10 , C12Q1/04 , G01N33/5017 , G01N33/54366 , G01N33/553 , G01N33/56911 , G01N33/56916 , G01N33/56938
摘要： The present invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof. More particularly, the invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof, the slide chip comprising: a substrate coated with a metal; a linker having a substituent which may be bonded to the metal and is located at the 5′ end of deoxythymidine (dT); and a food-borne bacterium-derived RNA aptamer that is bound to the linker by the 3′-end poly A tail. The slide chip makes it possible to detect food-borne bacteria in a rapid and accurate manner.

3. 发明申请

US20100239464A1 SUBSTRATE MANUFACTURING METHOD FOR SENSOR APPLICATIONS USING OPTICAL CHARACTERISTICS AND THE SUBSTRATE THEREFROM 有权
标题翻译：使用光学特性和基板的传感器应用的基板制造方法
公开(公告)号：US20100239464A1
公开(公告)日：2010-09-23
申请号：US12741389
申请日：2008-10-23
申请人： Yong Jin Cho , Chul Jin Kim , Chong Tai Kim , Sung Wook Choi , Jae Ho Kim , Hyo Sop Kim , Jin Ho Kim
发明人： Yong Jin Cho , Chul Jin Kim , Chong Tai Kim , Sung Wook Choi , Jae Ho Kim , Hyo Sop Kim , Jin Ho Kim
IPC分类号： G01N21/55 , C23C16/44 , B32B15/04 , B32B5/16 , G01N33/543
CPC分类号： G01N21/658 , G01N21/554 , Y10T428/24372 , Y10T428/31678
摘要： A method for manufacturing a substrate of an analytical sensor and the substrate thus prepared are disclosed. The method for manufacturing the substrate of the sensor application according to the present invention is characterized in that it comprises (a) the step of preparing a dispersed solution of nanoparticles, which are stable in a volatile organic solvent due to surface modification of nanoparticles having a pre-designed certain size on the nanometer level with an organic functional group (b) the step of preparing a single layer film of nanoparticles surface-modified with the organic functional group on the interface using said dispersed solution of nanoparticles on the basis of the Langmuir-Blodgett method, and then transferring said single layer film of nanoparticles to the substrate; and (c) the step of coating the substrate to which said single layer film of nanoparticles is transferred, with the metal thin film by means of the vacuum vapor deposition, and then optionally removing nanoparticles to manufacture a nanostructure to be used as the analytical sensor using optical characteristics. According to the method for manufacturing the substrate of the sensor application according to the present invention as above, the nanoparticles can be uniformly fixed on the solid substrate having a great area above 10×10 cm2 using the Langmuir-Blodgett method, and by such method the size, distance and shape of nanoparticles can be controlled to manufacture the nanostructures to be used as the analytical sensor, which is possible to reproduce and mass-produce. When the sensitivity property of the sensor is measured using the nanostructure substrate, thus produced, to be used as the analytical sensor, it can be identified that the sensitivity can be highly improved.
摘要翻译：公开了一种用于制造如此制备的分析传感器和衬底的衬底的方法。根据本发明的传感器应用的基板的制造方法的特征在于，其包括（a）制备纳米颗粒的分散溶液的步骤，所述分散溶液由于纳米颗粒的表面改性而在挥发性有机溶剂中稳定，用有机官能团预先设计在纳米级上的一定尺寸（b）制备在界面上用有机官能团表面改性的纳米颗粒单层薄膜的步骤，该纳米粒子基于Langmuir -Blodgett方法，然后将所述单层纳米颗粒膜转移到基底; 和（c）通过真空气相沉积用金属薄膜涂覆纳米颗粒的单层膜转移到其上的基底的步骤，然后任选地除去纳米颗粒以制造用作分析传感器的纳米结构使用光学特性。根据如上所述的根据本发明的传感器应用的基板的制造方法，可以使用Langmuir-Blodgett方法将纳米颗粒均匀地固定在具有大于10×10cm 2的大面积的固体基板上，并且通过这种方法可以控制纳米颗粒的尺寸，距离和形状，以制造用作分析传感器的纳米结构，其可以再现和批量生产。当使用由此产生的纳米结构基板测量传感器的灵敏度特性作为分析传感器时，可以确定可以高度提高灵敏度。

4. 发明申请

US20140287424A1 SLIDE CHIP FOR DETECTION SENSOR OF FOOD-BORNE PATHOGENS AND PREPARATION METHOD THEREOF 审中-公开
标题翻译：用于食物杆菌病原体检测传感器的滑动片及其制备方法
公开(公告)号：US20140287424A1
公开(公告)日：2014-09-25
申请号：US14343213
申请日：2012-02-22
申请人： Young Jin Cho , Chul Jin Kim , Nam Soo Kim , Chong Tai Kim , Jin Soo Maeng , Tae Eun Kim , Myung Hyun Lee
发明人： Young Jin Cho , Chul Jin Kim , Nam Soo Kim , Chong Tai Kim , Jin Soo Maeng , Tae Eun Kim , Myung Hyun Lee
IPC分类号： C12Q1/68 , G01N33/569
CPC分类号： C12Q1/689 , C12N15/111 , C12N2310/16 , C12N2320/10 , C12Q1/04 , G01N33/5017 , G01N33/54366 , G01N33/553 , G01N33/56911 , G01N33/56916 , G01N33/56938
摘要： The present invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof. More particularly, the invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof, the slide chip comprising: a substrate coated with a metal; a linker having a substituent which may be bonded to the metal and is located at the 5′ end of deoxythymidine (dT); and a food-borne bacterium-derived RNA aptamer that is bound to the linker by the 3′-end poly A tail. The slide chip makes it possible to detect food-borne bacteria in a rapid and accurate manner.
摘要翻译：本发明涉及一种用于检测食源性细菌的传感器的滑动片及其制造方法。更具体地说，本发明涉及一种用于检测食源性细菌的传感器的滑动芯片及其制造方法，该滑动芯片包括：涂覆有金属的基板; 具有可以与金属键合并位于脱氧胸苷（dT）的5'端的取代基的连接体; 以及通过3'末端聚A尾部与接头结合的食源性细菌衍生的RNA适配体。幻灯片使得可以以快速和准确的方式检测食源性细菌。

5. 发明申请

US20130029428A1 PREPARATION METHOD OF ANTIGEN-IMMOBILIZED IMMUNO- FLUORESCENCE SLIDE AND IMMUNO-FLUOROSCENCE SLIDE PREPARED THEREBY 审中-公开
标题翻译：抗原免疫荧光镜和免疫荧光镜的制备方法
公开(公告)号：US20130029428A1
公开(公告)日：2013-01-31
申请号：US13261437
申请日：2011-02-21
申请人： Nam Soo Kim , Yong Jin Cho , Chong Tai Kim
发明人： Nam Soo Kim , Yong Jin Cho , Chong Tai Kim
IPC分类号： G01N21/64 , C07K17/02 , B82Y15/00
CPC分类号： G01N33/54366
摘要： A method of preparing an antigen-immobilized immuno-fluorescence slide, the method comprising: immobilizing a C-reactive protein on a slide to prepare a protein chip; mixing an antibody that specifically binds to a target protein, with streptavidin to label the antibody with a fluorescent nanoparticle; immuno-reacting the antibody by competitive mixing, assaying with a fluorescence camera, wherein the immobilizing of the C-reactive protein on the slide comprises: modifying the slide with 3-aminopropyltrimethoxysilane to prepare a modified slide; hydrating the slide modified with 3-aminopropyltrimethoxysilane; activating the modified slide by using a glutaraldehyde solution; dissolving a C-reactive protein at a concentration of 0.01-0.5 mg/ml in a 30-70 mM phosphate buffer solution (pH 6.5-7.8) to prepare an antigen solution for immobilization; placing a petri dish comprising the slide on a spotting guide and spotting 1-100 μl of the antigen solution on spotting points; and performing a reaction on the slide prepared as described above for 1-6 hours to immobilize the antigen, and an immune-fluorescence slide prepared by using the method.
摘要翻译：一种制备抗原固定化免疫荧光载片的方法，所述方法包括：将C反应蛋白固定在载玻片上以制备蛋白质芯片; 将特异性结合靶蛋白的抗体与链霉抗生物素蛋白混合以用荧光纳米颗粒标记抗体; 通过竞争性混合使抗体免疫反应，用荧光相机进行测定，其中将C-反应蛋白固定在载玻片上包括：用3-氨基丙基三甲氧基硅烷改性载玻片以制备修饰的载玻片; 水化用3-氨基丙基三甲氧基硅烷改性的载玻片; 通过使用戊二醛溶液活化修饰的载玻片; 在30-70mM磷酸盐缓冲溶液（pH6.5-7.8）中溶解浓度为0.01-0.5mg / ml的C-反应蛋白以制备用于固定的抗原溶液; 将包含载玻片的陪替氏培养皿置于点样引导物上，并在点样点上点样1-100μl的抗原溶液; 并对如上所述制备的载玻片进行1-6小时的反应以固定抗原，以及通过使用该方法制备的免疫荧光载片。

6. 发明申请

US20100136207A1 NANOEMULSION AND NANOPARTICLE CONTAINING PLANT ESSENTIAL OIL AND METHOD OF PRODUCTION THEREOF 审中-公开
标题翻译：含有植物油的纳米油和纳米油及其生产方法
公开(公告)号：US20100136207A1
公开(公告)日：2010-06-03
申请号：US12452934
申请日：2008-06-11
申请人： Chong Tai Kim , Chul Jin Kim , Yong Jin Cho , Sung Wook Choi , Aei Jin Choi
发明人： Chong Tai Kim , Chul Jin Kim , Yong Jin Cho , Sung Wook Choi , Aei Jin Choi
IPC分类号： A23L1/222 , A23L1/22
CPC分类号： A23L33/105 , A23L27/10 , A23L29/10 , A23L29/275 , A23V2002/00 , A23V2200/25 , A23V2200/222 , A23V2250/511
摘要： The present invention relates to a nano emulsion capable of reducing an intestinal stimulus effect, increasing the stability and absorptance of the functional components in food, and solving reluctance when ingesting by reducing a strong flavor capable of being generated by the functional components, by making the functional components of food into a nano emulsion using hydrophilic biopolymer, and a nano particle capable of further increasing the stability by performing solvent precipitation or vacuum freeze drying on the nano emulsion and capable of being easily applied to other food, and a method of production thereof.
摘要翻译：本发明涉及能够降低肠刺激作用，增加食品中功能成分的稳定性和吸收性的纳米乳液，以及通过减少能够由功能成分产生的强烈香味来摄取时的难度，通过使使用亲水性生物聚合物的食品的功能成分成纳米乳液，以及能够通过在纳米乳液上进行溶剂沉淀或真空冷冻干燥并能够容易地应用于其他食品而进一步提高稳定性的纳米粒子及其制备方法。

7. 发明授权

US09045744B2 Method for using an enzyme resistant to high pressures 有权
标题翻译：使用耐高压酶的方法
公开(公告)号：US09045744B2
公开(公告)日：2015-06-02
申请号：US14000497
申请日：2012-02-20
申请人： Nam-Soo Kim , Chong-Tai Kim , Yong-Jin Cho , Chul-Jin Kim , Jin-Soo Maeng , Soo-Jin Kwon
发明人： Nam-Soo Kim , Chong-Tai Kim , Yong-Jin Cho , Chul-Jin Kim , Jin-Soo Maeng , Soo-Jin Kwon
IPC分类号： C12N9/50 , C12Q1/37 , C12N9/64 , A61K38/48 , A61K38/51 , A23L1/01 , A23L1/03 , A23L1/22 , A23L1/227 , A23L3/015 , C12N9/48 , C12N9/52 , C12N9/76
CPC分类号： C12N9/6481 , A23L3/0155 , A23L5/17 , A23L27/00 , A23L27/21 , A23L29/06 , A61K38/48 , A61K38/51 , C12N9/48 , C12N9/485 , C12N9/52 , C12N9/6427 , C12Q1/37 , C12Y304/00
摘要： At least at least one embodiment of the present invention relates to a method for using a high pressure-resistant enzyme in a high pressure condition; a method for promoting the activity of the high pressure-resistant enzyme by means of a high pressure treatment; a composition, which contains the high pressure-resistant enzyme, for decomposing proteins under a high pressure condition; a composition, which contains the composition for decomposing proteins, for preparing natural flavoring substances; a container for high pressure treatment, which contains the composition for decomposing proteins; and a method for measuring the activity of the high pressure-resistant enzyme, which comprises a step of decomposing an azocasein solution serving as a substrate by using the high pressure-resistant enzyme treated under a high pressure condition.
摘要翻译：本发明的至少一个实施方案涉及在高压条件下使用高耐压酶的方法; 通过高压处理促进高耐压酶的活性的方法; 含有高耐压酶的组合物，用于在高压条件下分解蛋白质; 含有用于分解蛋白质的组合物的组合物，用于制备天然调味物质; 用于高压处理的容器，其包含用于分解蛋白质的组合物; 以及测定高耐压酶的活性的方法，其包括通过使用在高压条件下处理的高耐压酶分解作为底物的偶氮酪蛋白溶液的步骤。

8. 发明授权

US08414965B2 Substrate manufacturing method for sensor applications using optical characteristics and the substrate therefrom 有权
标题翻译：使用光学特性的传感器应用的基板制造方法及其基板
公开(公告)号：US08414965B2
公开(公告)日：2013-04-09
申请号：US12741389
申请日：2008-10-23
申请人： Yong Jin Cho , Chul Jin Kim , Chong Tai Kim , Sung Wook Choi , Jae Ho Kim , Hyo Sop Kim , Jin Ho Kim
发明人： Yong Jin Cho , Chul Jin Kim , Chong Tai Kim , Sung Wook Choi , Jae Ho Kim , Hyo Sop Kim , Jin Ho Kim
IPC分类号： B05D5/12 , C23C14/28 , C23C14/30 , C23C14/14 , H05B6/00 , H05B7/00 , B05D1/18 , B05D3/00
CPC分类号： G01N21/658 , G01N21/554 , Y10T428/24372 , Y10T428/31678
摘要： A method for manufacturing a substrate of an analytical sensor and the substrate thus prepared are disclosed. The method for manufacturing the substrate of the sensor application according to the present invention is characterized in that it comprises (a) the step of preparing a dispersed solution of nanoparticles, which are stable in a volatile organic solvent due to surface modification of nanoparticles having a pre-designed certain size on the nanometer level with an organic functional group (b) the step of preparing a single layer film of nanoparticles surface-modified with the organic functional group on the interface using said dispersed solution of nanoparticles on the basis of the Langmuir-Blodgett method, and then transferring said single layer film of nanoparticles to the substrate; and (c) the step of coating the substrate to which said single layer film of nanoparticles is transferred, with the metal thin film by means of the vacuum vapor deposition, and then optionally removing nanoparticles to manufacture a nanostructure to be used as the analytical sensor using optical characteristics. According to the method for manufacturing the substrate of the sensor application according to the present invention as above, the nanoparticles can be uniformly fixed on the solid substrate having a great area above 10×10 cm2 using the Langmuir-Blodgett method, and by such method the size, distance and shape of nanoparticles can be controlled to manufacture the nanostructures to be used as the analytical sensor, which is possible to reproduce and mass-produce. When the sensitivity property of the sensor is measured using the nanostructure substrate, thus produced, to be used as the analytical sensor, it can be identified that the sensitivity can be highly improved.
摘要翻译：公开了一种用于制造如此制备的分析传感器和衬底的衬底的方法。根据本发明的传感器应用的基板的制造方法的特征在于，其包括（a）制备纳米颗粒的分散溶液的步骤，所述分散溶液由于纳米颗粒的表面改性而在挥发性有机溶剂中稳定，用有机官能团预先设计在纳米级上的一定尺寸（b）制备在界面上用有机官能团表面改性的纳米颗粒单层薄膜的步骤，该纳米粒子基于Langmuir -Blodgett方法，然后将所述单层纳米颗粒膜转移到基底; 和（c）通过真空气相沉积用金属薄膜涂覆纳米颗粒的单层膜转移到其上的基底的步骤，然后任选地除去纳米颗粒以制造用作分析传感器的纳米结构使用光学特性。根据如上所述的根据本发明的传感器应用的基板的制造方法，可以使用Langmuir-Blodgett方法将纳米颗粒均匀地固定在具有大于10×10cm 2的大面积的固体基板上，并且通过这种方法可以控制纳米颗粒的尺寸，距离和形状，以制造用作分析传感器的纳米结构，其可以再现和批量生产。当使用由此产生的纳米结构基板测量传感器的灵敏度特性作为分析传感器时，可以确定可以高度提高灵敏度。

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