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1. 发明授权

US4495294A Method for determining bacterial endotoxin and kit therefor 失效
标题翻译：确定细菌内毒素的方法及其试剂盒
公开(公告)号：US4495294A
公开(公告)日：1985-01-22
申请号：US441810
申请日：1982-11-15
申请人： Chizuko Nakahara , Shigenori Tanaka , Hiroshi Tamura , Akiyoshi Matsumoto
发明人： Chizuko Nakahara , Shigenori Tanaka , Hiroshi Tamura , Akiyoshi Matsumoto
IPC分类号： G01N33/52 , G01N33/50 , G01N33/579 , C12Q1/00 , C12Q1/36 , C12Q1/38
CPC分类号： G01N33/579 , Y10S435/81 , Y10T436/25125
摘要： Bacterial endotoxin in an assay sample is determined by an amoebocyte lysate component of horseshoe crab in the presence or absence of a substrate for endotoxin determination by using an assay sample in the liquid state obtained by treating said assay sample with an endotoxin-free acid having a pKa.sup.25.degree. C. value of not more than 3 at a pH of not more than 3; and a kit therefor, a receptacle containing (a) an endotoxin-free acid having a pKa.sup.25.degree. C. value of not more than 3 e.g. perchloric acid, trichloroacetic acid, nitric acid, for treating the assay sample and a receptacle containing (b) an amoebocyte lysate component or horseshoe crab. The acid treatment of the assay sample results in improved accuracy, reliability and reproducibility of the bacterial endotoxin determination.
摘要翻译：测定样品中的细菌内毒素通过在存在或不存在用于内毒素测定的底物的存在或不存在下测定的测定样品中的细菌内毒素，其通过使用通过用不含内毒素的酸处理所述测定样品获得的液体状态的测定样品，所述测定样品具有 pKa25℃，pH值不大于3时不大于3; 和一种试剂盒，其容器含有（a）pKa25℃值不大于3的无内毒素的酸，例如，高氯酸，三氯乙酸，硝酸，用于处理测定样品和含有（b）变形细胞裂解物组分或马蹄蟹的容器。测定样品的酸处理提高了细菌内毒素测定的准确性，可靠性和再现性。

2. 发明授权

US4038141A Methods for extracting and purifying kallidinogenase 失效
标题翻译：提取和净化KALLIDINOGENASE的方法
公开(公告)号：US4038141A
公开(公告)日：1977-07-26
申请号：US674645
申请日：1976-04-07
申请人： Kiyochika Tokuyasu , Takeshi Yokobori , Chizuko Nakahara
发明人： Kiyochika Tokuyasu , Takeshi Yokobori , Chizuko Nakahara
IPC分类号： C12N9/64 , C07G7/026 , C12D13/10
CPC分类号： C12N9/6445 , Y10S435/815
摘要： A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45.degree. to about 60.degree. C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.

3. 发明授权

US3994782A Methods for extracting and purifying kallidinogenase 失效
标题翻译：提取和纯化杀鼠肽素酶的方法
公开(公告)号：US3994782A
公开(公告)日：1976-11-30
申请号：US638042
申请日：1975-12-05
申请人： Kiyochika Tokuyasu , Takeshi Yokobori , Chizuko Nakahara
发明人： Kiyochika Tokuyasu , Takeshi Yokobori , Chizuko Nakahara
IPC分类号： C12N9/64 , C07G7/026 , A61K37/48
CPC分类号： C12N9/6445 , Y10S435/814 , Y10S435/815
摘要： A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45.degree. to about 60.degree. C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
摘要翻译：一种提取和收集杀鼠肽素酶（EC 3.4.4.21物质）的方法，其包括在盐存在下，在约45℃至约60℃的温度和约5至约5℃的温度下用水萃取哺乳动物的胰腺，约8，其中所述提取在镁的水溶性盐与酸的存在下进行，以及纯化杀青霉素酶的方法，该方法包括使水相从哺乳动物的胰腺获得的含有杀线索杆菌素酶的水提取物使用阴离子交换树脂进行吸附洗脱处理，其中使所述含有杀线虫素原的水提取物的所述水相与凝胶型弱碱性阴离子交换树脂接触，以使所述蓝霉素酶部分被所述树脂吸附，并用弱酸的铵盐的水溶液洗脱苍耳蛋白原酶，从而收集苍白球素原酶。

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