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1. 发明申请

US20180147263A1 METHOD OF TREATING NEUROLOGICAL DISORDER BY ENHANCING OUTGROWTH OF NEURITE 审中-公开
公开(公告)号：US20180147263A1
公开(公告)日：2018-05-31
申请号：US15604654
申请日：2017-05-25
申请人： Huai-Jen TSAI , Cheng-Yung LIN
发明人： Huai-Jen TSAI , Cheng-Yung LIN , Kok-Zhi LEE , You-Jei CHEN
IPC分类号： A61K38/45 , A61K31/428 , A61K39/395 , A61K31/4545 , A61K31/4985 , A61K31/13 , A61K31/445 , A61K31/55 , A61K31/325
CPC分类号： A61K38/45 , A61K31/13 , A61K31/27 , A61K31/325 , A61K31/428 , A61K31/445 , A61K31/4545 , A61K31/4985 , A61K31/55 , A61K39/3955 , A61K45/06 , A61P25/00 , C07K16/40 , C07K2317/00 , C07K2317/74 , C07K2317/76 , C12Y207/02003 , A61K2300/00
摘要： Disclosed herein is a method of enhancing the outgrowth of the neurite of a neuron in a subject. The present method comprises administering to the subject a therapeutically effective amount of phosphoglycerate kinase (PGK). Based on the enhancing effect, also disclosed herein is a method of treating a neurological disorder in a subject by use of the PGK or a pharmaceutical composition comprising the PGK and an active agent.

2. 发明申请

US20120264124A1 MICROSATELLITE MARKER COMBINATION AND METHOD FOR IDENTIFYING LANYU PIG BREED 审中-公开
标题翻译：微生物标记组合及鉴定方法
公开(公告)号：US20120264124A1
公开(公告)日：2012-10-18
申请号：US13493016
申请日：2012-06-11
申请人： YU-TEN JU , YAN-NIAN JIANG , HSIEN-PING CHU , KUEN-JAW CHEN , WEI-HUA CHANG , SHOU-HSIEN LI , YING WANG , CHIA-HSUAN CHEN , Cheng-Yung LIN
发明人： YU-TEN JU , YAN-NIAN JIANG , HSIEN-PING CHU , KUEN-JAW CHEN , WEI-HUA CHANG , SHOU-HSIEN LI , YING WANG , CHIA-HSUAN CHEN , Cheng-Yung LIN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6888 , C12Q2600/156
摘要： The present invention provides a microsatellite marker combination for identifying Lanyu pig breed, and the identification method thereof. The identification method comprises the following steps: (a) providing a genomic DNA sample obtained from a pig; (b) identifying the polymorphism of microsatellite markers of said genomic DNA sample; and (c) analyzing the results obtained from step (b) to determine the phylogenetic relationship between said pig and Lanyu pig.
摘要翻译：本发明提供了一种用于鉴定兰屿猪品种的微卫星标记组合及其鉴定方法。鉴定方法包括以下步骤：（a）提供从猪获得的基因组DNA样品; （b）鉴定所述基因组DNA样品的微卫星标记的多态性; 和（c）分析从步骤（b）获得的结果，以确定所述猪和兰屿猪之间的系统发育关系。

3. 发明申请

US20120054880A1 METHOD FOR ENHANCING PRODUCTION OF PATHOGEN-RESISTANT PROTEINS USING BIOREACTOR 审中-公开
标题翻译：使用生物反应器增强抗病原蛋白生产的方法
公开(公告)号：US20120054880A1
公开(公告)日：2012-03-01
申请号：US13216396
申请日：2011-08-24
申请人： HUAI-JEN TSAI , CHENG-YUNG LIN
发明人： HUAI-JEN TSAI , CHENG-YUNG LIN
IPC分类号： C12N15/63
CPC分类号： C12N15/67 , A01K67/0275 , A01K2217/00 , A01K2227/40 , A01K2267/01
摘要： A method for enhancing production of pathogen-resistant proteins using bioreactor comprising: (a) constructing a plasmid which comprises a nucleic acid sequence of exogenous pathogen-resistant protein, a nucleic acid sequence of fluorescence protein, a nucleic acid sequence of protease-cutting site and an α-actin promoter; (b) transferring the constructed plasmid into fish embryo(s) of selected line after linearization; (c) domesticating the fish to enable to grow, mate and spawn; and (d) collecting fish egg(s), fish embryo(s) and adult fish(s) to obtain pathogen-resistant protein-rich organism(s) is described. A nucleic acid construct comprising an α-actin promoter, a nucleic acid sequence of exogenous pathogen-resistant protein and a nucleic acid sequence of fluorescence protein which are operably linked in the 5′ to 3′ direction is also presented. An expression vector comprising said nucleic acid construct is further described.
摘要翻译：一种使用生物反应器增强病原体抗性蛋白质生产的方法，包括：（a）构建质粒，其包含外源性病原体抗性蛋白质的核酸序列，荧光蛋白的核酸序列，蛋白酶切割位点的核酸序列和α-肌动蛋白启动子; （b）线性化后将构建的质粒转移到选定品系的鱼胚中; （c）驯养鱼以使其生长，交配和产卵; 描述了（d）收集鱼卵，鱼胚和成年鱼以获得富含病原体的富含蛋白质的生物体。还提出了包含α-肌动蛋白启动子，外源性病原体抗性蛋白的核酸序列和荧光蛋白的核酸序列的核酸构建体，其在5'至3'方向上可操作地连接。进一步描述了包含所述核酸构建体的表达载体。

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