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1. 发明授权

US08281672B2 Automatable aseptic sample withdrawal system 有权
标题翻译：自动无菌取样系统
公开(公告)号：US08281672B2
公开(公告)日：2012-10-09
申请号：US12727719
申请日：2010-03-19
申请人： Chanyong Brian Lee , Daniel Giroux
发明人： Chanyong Brian Lee , Daniel Giroux
IPC分类号： G01N1/00
CPC分类号： G01N1/14 , C12M33/06 , C12M37/02 , C12M41/00 , G01N2001/1427
摘要： An aseptic sampling system includes substantially sealed sampling lines connecting one or more sample container to a vessel containing the fluid to be sampled. A flow control system includes valves and/or clamps placed strategically along the sampling lines and filtered vent lines are provided at strategic locations relative to a reversible fluid pump in communication with the sampling lines. The clamps are selectively actuated, the vent lines are selectively opened or closed, and the pump is operated in a forward or reverse direction to permit a volume of sample fluid to be drawn from the vessel and into a sample container and to permit fluid to be purged from the sampling lines either into the sample container or back into the vessel. Thus, sampling and purging can be accomplished without opening the system to potentially contaminating agents and without wasting or having to properly dispose the fluid.
摘要翻译：无菌采样系统包括将一个或多个样品容器连接到容纳待采样流体的容器的基本上密封的采样管线。流量控制系统包括沿采样管道策略地放置的阀和/或夹具，并且在与采样管路连通的可逆流体泵的相关战略位置提供过滤的排气管线。选择性地启动夹具，排气管线被选择性地打开或关闭，并且泵在正向或反向方向上操作，以允许一定体积的样品流体从容器中抽出并进入样品容器并允许流体为从采样管线中清除或进入样品容器或回到容器中。因此，可以在不将系统打开到潜在的污染剂的情况下完成取样和清洗，而不会浪费或不必适当地处理流体。

2. 发明申请

US20080274452A1 Process For Large Scale Production Of Plasmid Dna By E.Coli Fermentation 有权
标题翻译：大肠杆菌发酵大规模生产质粒Dna的方法
公开(公告)号：US20080274452A1
公开(公告)日：2008-11-06
申请号：US10588295
申请日：2005-01-31
申请人： Michel Chartrain , Laura Kizer Bentley , Barbara Ann Krulewicz , Kristin M. Listner , Wen-jun Sun , Chanyong Brian Lee
发明人： Michel Chartrain , Laura Kizer Bentley , Barbara Ann Krulewicz , Kristin M. Listner , Wen-jun Sun , Chanyong Brian Lee
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12N1/20 , C12N15/69
摘要： The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.
摘要翻译：本发明一般涉及提高质粒DNA产量的方法。该方法包括以下步骤：选择大肠杆菌菌株的高产克隆亚型，包括但不限于携带DNA质粒的DH5菌株，并且在化学定义的培养基中用补料分批发酵培养所述克隆亚型。当所述高产克隆亚型在工业规模上培育时，本文所述的质粒DNA生产方法可产生质量DNA的记录数量。所公开的方法可用于生产用于多核苷酸疫苗接种和基因治疗治疗方案的药物级DNA。

3. 发明授权

US08603798B2 Process for large scale production of plasmid DNA by E. coli fermentation 有权
标题翻译：通过大肠杆菌发酵大规模生产质粒DNA的方法
公开(公告)号：US08603798B2
公开(公告)日：2013-12-10
申请号：US13190137
申请日：2011-07-25
申请人： Michel Chartrain , Laura Kizer Bentley , Barbara Ann Krulewicz , Kristin M. Listner , Wen-jun Sun , Chanyong Brian Lee
发明人： Michel Chartrain , Laura Kizer Bentley , Barbara Ann Krulewicz , Kristin M. Listner , Wen-jun Sun , Chanyong Brian Lee
IPC分类号： C12N1/20 , C12N15/63
CPC分类号： C12P19/34 , C12N1/20 , C12N15/69
摘要： The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.
摘要翻译：本发明一般涉及提高质粒DNA产量的方法。该方法包括以下步骤：选择大肠杆菌菌株的高产克隆亚型，包括但不限于携带DNA质粒的DH5菌株，并且在化学定义的培养基中用补料分批发酵培养所述克隆亚型。当所述高产克隆亚型在工业规模上培育时，本文所述的质粒DNA生产方法可产生质量DNA的记录数量。所公开的方法可用于生产用于多核苷酸疫苗接种和基因治疗治疗方案的药物级DNA。

4. 发明申请

US20090269849A1 Bioreactor Apparatus 审中-公开
标题翻译：生物反应器装置
公开(公告)号：US20090269849A1
公开(公告)日：2009-10-29
申请号：US12428778
申请日：2009-04-23
申请人： Chanyong Brian LEE , Joseph Gregory ZEIKUS
发明人： Chanyong Brian LEE , Joseph Gregory ZEIKUS
IPC分类号： C12N5/00 , C12M1/00 , C12N1/00
CPC分类号： B01F7/00308 , B01F3/04588 , B01F7/00916 , B01F2003/04673 , C12M23/14 , C12M27/06 , C12M29/20 , C12P5/023 , Y02E50/343
摘要： A bioreactor includes a fluid containment vessel and a rotating mixing element including radial inflow elements configured to direction fluid radially inwardly of the mixing element and axial out flow elements configured to redirect the radial inward flow in an axial direction. Both the vessel and the mixing element are made from plastic and the vessel comprises a flexible bag supported by a rigid housing. In one embodiment, the mixing element is pneumatically driven by the buoyancy of gas introduced into the vessel and trapped within gas entrapment cups formed on the periphery of the mixing element. As the volumetric capacity of the bioreactor increases, the ratio of the diameter of the mixing element to the width of the vessel decreases, and a gap defined between the bottom of the mixing element and the bottom of the vessel increase.
摘要翻译：生物反应器包括流体容纳容器和旋转混合元件，旋转混合元件包括径向流入元件，其被配置为朝向混合元件的径向向内的流体的方向，并且轴向流出流动元件构造成沿径向向内流动沿轴向重定向。容器和混合元件均由塑料制成，并且容器包括由刚性壳体支撑的柔性袋。在一个实施例中，混合元件由引入到容器中的气体的浮力气动地驱动，并被捕获在形成在混合元件的周边上的气体捕获杯内。随着生物反应器的体积增加，混合元件的直径与容器宽度的比率减小，并且在混合元件的底部和容器的底部之间限定的间隙增加。

5. 发明申请

US20120190073A1 PROCESS FOR LARGE SCALE PRODUCTION OF PLASMID DNA BY E. COLI FERMENTATION 有权
标题翻译：大肠杆菌发酵大规模生产PLASMID DNA的方法
公开(公告)号：US20120190073A1
公开(公告)日：2012-07-26
申请号：US13190137
申请日：2011-07-25
申请人： Michel CHARTRAIN , Laura Kizer BENTLEY , Barbara Ann KRULEWICZ , Kristin M. LISTNER , Wen-jun SUN , Chanyong Brian LEE
发明人： Michel CHARTRAIN , Laura Kizer BENTLEY , Barbara Ann KRULEWICZ , Kristin M. LISTNER , Wen-jun SUN , Chanyong Brian LEE
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N1/20 , C12N15/69
摘要： The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.
摘要翻译：本发明一般涉及提高质粒DNA产量的方法。该方法包括以下步骤：选择大肠杆菌菌株的高产克隆亚型，包括但不限于携带DNA质粒的DH5菌株，并且在化学定义的培养基中用补料分批发酵培养所述克隆亚型。当所述高产克隆亚型在工业规模上培育时，本文所述的质粒DNA生产方法可产生质量DNA的记录数量。所公开的方法可用于生产用于多核苷酸疫苗接种和基因治疗治疗方案的药物级DNA。

6. 发明授权

US07998732B2 Process for large scale production of plasmid DNA by E. coli fermentation 有权
标题翻译：通过大肠杆菌发酵大规模生产质粒DNA的方法
公开(公告)号：US07998732B2
公开(公告)日：2011-08-16
申请号：US10588295
申请日：2005-01-31
申请人： Michel Chartrain , Laura Kizer Bentley , Barbara Ann Krulewicz , Kristin M. Listner , Wen-jun Sun , Chanyong Brian Lee
发明人： Michel Chartrain , Laura Kizer Bentley , Barbara Ann Krulewicz , Kristin M. Listner , Wen-jun Sun , Chanyong Brian Lee
IPC分类号： C12N15/63 , C12N1/21 , C12N15/70
CPC分类号： C12P19/34 , C12N1/20 , C12N15/69
摘要： The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.
摘要翻译：本发明一般涉及提高质粒DNA产量的方法。该方法包括以下步骤：选择大肠杆菌菌株的高产克隆亚型，包括但不限于携带DNA质粒的DH5菌株，并且在化学定义的培养基中用补料分批发酵培养所述克隆亚型。当所述高产克隆亚型在工业规模上培育时，本文所述的质粒DNA生产方法可产生记录量的质粒DNA。所公开的方法可用于生产用于多核苷酸疫苗接种和基因治疗治疗方案的药物级DNA。

7. 发明申请

US20100236340A1 AUTOMATABLE ASEPTIC SAMPLE WITHDRAWAL SYSTEM 有权
标题翻译：自动抽样抽样系统
公开(公告)号：US20100236340A1
公开(公告)日：2010-09-23
申请号：US12727719
申请日：2010-03-19
申请人： Chanyong Brian LEE , Daniel GIROUX
发明人： Chanyong Brian LEE , Daniel GIROUX
IPC分类号： G01N1/14
CPC分类号： G01N1/14 , C12M33/06 , C12M37/02 , C12M41/00 , G01N2001/1427
摘要： An aseptic sampling system includes substantially sealed sampling lines connecting one or more sample container to a vessel containing the fluid to be sampled. A flow control system includes valves and/or clamps placed strategically along the sampling lines and filtered vent lines are provided at strategic locations relative to a reversible fluid pump in communication with the sampling lines. The clamps are selectively actuated, the vent lines are selectively opened or closed, and the pump is operated in a forward or reverse direction to permit a volume of sample fluid to be drawn from the vessel and into a sample container and to permit fluid to be purged from the sampling lines either into the sample container or back into the vessel. Thus, sampling and purging can be accomplished without opening the system to potentially contaminating agents and without wasting or having to properly dispose the fluid.
摘要翻译：无菌采样系统包括将一个或多个样品容器连接到容纳待采样流体的容器的基本上密封的采样管线。流量控制系统包括沿采样管道策略地放置的阀和/或夹具，并且在与采样管路连通的可逆流体泵的相关战略位置提供过滤的排气管线。选择性地启动夹具，排气管线被选择性地打开或关闭，并且泵在正向或反向方向上操作，以允许一定体积的样品流体从容器中抽出并进入样品容器并允许流体为从采样管线中清除或进入样品容器或回到容器中。因此，可以在不将系统打开到潜在的污染剂的情况下完成取样和清洗，而不会浪费或不必适当地处理流体。

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