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1. 发明授权

US08334429B2 Auxotrophic Agrobacterium for plant transformation and methods thereof 失效
标题翻译：用于植物转化的营养型农杆菌及其方法
公开(公告)号：US08334429B2
公开(公告)日：2012-12-18
申请号：US12650106
申请日：2009-12-30
申请人： Jerome P. Ranch , Matthias Liebergesell , Carl W. Garnaat , Gary A. Huffman
发明人： Jerome P. Ranch , Matthias Liebergesell , Carl W. Garnaat , Gary A. Huffman
IPC分类号： C12N15/84 , C12N15/74 , C12N1/20 , C12N1/21
CPC分类号： C12N15/8205 , C12N9/1007
摘要： Auxotrophic Agrobacterium and methods employing auxotrophic Agrobacterium are provided. Auxotrophic Agrobacterium may be used in a variety of methods including biologically containing Agrobacterium comprising a transgene and transforming a plant cell without using an Agrobacterium counter-selective agent. Transforming maize immature embryos using an Agrobacterium auxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic Agrobacterium. Methods for producing an Agrobacterium thymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.
摘要翻译：营养缺陷型农杆菌和使用营养缺陷型农杆菌的方法。营养不良农杆菌可以以多种方法使用，包括生物地含有转基因的农杆菌，并且不使用农杆菌反选择剂来转化植物细胞。使用农杆菌营养缺陷型用于胸苷转化玉米未成熟胚，导致与使用原养性农杆菌的转化相当的转化效率。公开了用于生产农杆菌营养缺陷型农杆菌和在转化方法中使用营养缺陷型的方法。还提供了使用本发明的方法生产的转化的组织和植物。

2. 发明授权

US08618358B2 Transgenic maize event MON 87427 and the relative development scale 有权
标题翻译：转基因玉米事件MON 87427及相对发展规模
公开(公告)号：US08618358B2
公开(公告)日：2013-12-31
申请号：US12947454
申请日：2010-11-16
申请人： Paul C. C. Feng , Agustin E. Fonseca , Carl W. Garnaat , Oscar Heredia , Jintai Huang , Rebecca A. Kelly , Youlin Qi , Martin A. Stoecker
发明人： Paul C. C. Feng , Agustin E. Fonseca , Carl W. Garnaat , Oscar Heredia , Jintai Huang , Rebecca A. Kelly , Youlin Qi , Martin A. Stoecker
IPC分类号： C12N15/82 , C12N15/52 , C12N15/11 , C12Q1/68 , A01H9/00
CPC分类号： A01H5/10 , A01H1/02 , C12N15/8275 , C12N15/8289 , C12Q1/6895 , Y02E50/10 , Y02E50/30 , Y10T436/143333
摘要： The invention provides transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87427. The invention also provides nucleotides specific for transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products comprising nucleotides specific for transgenic maize event MON 87427. The invention also provides methods related to transgenic maize event MON 87427 and to the Roundup® Hybridization System (RHS). The invention also provides a Relative Development Scale useful for monitoring and determining reproductive development in maize that reconciles developmental differences across various maize varieties. This is useful for determining the optimal timing of a treatment regimen in which tassel development stage is an important factor, including various methods in making hybrid seed.
摘要翻译：本发明提供转基因玉米事件MON 87427和源自事件MON 87427的植物，植物细胞，种子，植物部分和商品。本发明还提供转基因玉米事件MON 87427的特异性核苷酸和植物，植物细胞，种子，植物部分以及包含对转基因玉米事件MON 87427具有特异性的核苷酸的商品。本发明还提供了与转基因玉米事件MON 87427和综合杂交系统（RHS）相关的方法。本发明还提供了一个有助于监测和确定玉米生殖发育的相对发展规模，以调节各种玉米品种间的发育差异。这可用于确定流苏开发阶段是重要因素的治疗方案的最佳时机，其中包括制作杂交种子的各种方法。

3. 发明申请

US20110126310A1 TRANSGENIC MAIZE EVENT MON 87427 AND THE RELATIVE DEVELOPMENT SCALE 有权
标题翻译：转基因事件MON 87427和相对发展规模
公开(公告)号：US20110126310A1
公开(公告)日：2011-05-26
申请号：US12947454
申请日：2010-11-16
申请人： Paul C.C. Feng , Agustin E. Fonseca , Carl W. Garnaat , Oscar Heredia , Jintai Huang , Rebecca A. Kelly , Martin A. Stoecker
发明人： Paul C.C. Feng , Agustin E. Fonseca , Carl W. Garnaat , Oscar Heredia , Jintai Huang , Rebecca A. Kelly , Martin A. Stoecker
IPC分类号： A01H5/00 , C07H21/04 , A01H5/10 , C12N5/04 , C11B1/00 , A01N57/20 , G01N33/53 , A01H1/04 , C12Q1/68 , A01P13/00 , A01P21/00 , C12Q1/02 , C10L5/44 , A01G1/00 , A01C7/00 , A01D46/00 , A23K1/00 , A23L1/10 , A23L1/164
CPC分类号： A01H5/10 , A01H1/02 , C12N15/8275 , C12N15/8289 , C12Q1/6895 , Y02E50/10 , Y02E50/30 , Y10T436/143333
摘要： The invention provides transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87427. The invention also provides nucleotides specific for transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products comprising nucleotides specific for transgenic maize event MON 87427. The invention also provides methods related to transgenic maize event MON 87427 and to the Roundup® Hybridization System (RHS). The invention also provides a Relative Development Scale useful for monitoring and determining reproductive development in maize that reconciles developmental differences across various maize varieties. This is useful for determining the optimal timing of a treatment regimen in which tassel development stage is an important factor, including various methods in making hybrid seed.
摘要翻译：本发明提供转基因玉米事件MON 87427和源自事件MON 87427的植物，植物细胞，种子，植物部分和商品。本发明还提供转基因玉米事件MON 87427的特异性核苷酸和植物，植物细胞，种子，植物部分以及包含对转基因玉米事件MON 87427具有特异性的核苷酸的商品。本发明还提供了与转基因玉米事件MON 87427和综合杂交系统（RHS）相关的方法。本发明还提供了一个有助于监测和确定玉米生殖发育的相对发展规模，以调节各种玉米品种间的发育差异。这可用于确定流苏开发阶段是重要因素的治疗方案的最佳时机，其中包括制作杂交种子的各种方法。

4. 发明授权

US5824864A Maize gene and protein for insect control 失效
标题翻译：玉米基因和蛋白质用于昆虫控制
公开(公告)号：US5824864A
公开(公告)日：1998-10-20
申请号：US449986
申请日：1995-05-25
申请人： Timothy W. Fox , Carl W. Garnaat , Terry EuClaire Meyer
发明人： Timothy W. Fox , Carl W. Garnaat , Terry EuClaire Meyer
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , C12N15/00 , A01H5/00
CPC分类号： C12N15/8286 , A01N65/00 , A01N65/44 , C07K14/415
摘要： Nucleotide sequences encoding a maize 5C9 polypeptide, the 5C9 polypeptide and structural and functional equivalents, and methods for controlling insect infestation in plants are provided.
摘要翻译：提供编码玉米5C9多肽的核苷酸序列，5C9多肽及其结构和功能等同物，以及控制植物昆虫侵袭的方法。

5. 发明授权

US06838593B2 Austin responsive promoter sequences and methods of using the same 失效
标题翻译：奥斯汀反应启动子序列及其使用方法
公开(公告)号：US06838593B2
公开(公告)日：2005-01-04
申请号：US09905558
申请日：2001-07-13
申请人： Carl W. Garnaat , Keith S. Lowe , Bradley A. Roth
发明人： Carl W. Garnaat , Keith S. Lowe , Bradley A. Roth
IPC分类号： C07K14/415 , C12N15/82 , C12N15/09 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8201 , C12N15/8209 , C12N15/8222 , C12N15/8289 , C12N15/8294
摘要： The invention provides isolated auxin-responsive transcriptional regulatory elements and polynucleotides associated therewith. The present invention provides methods and compositions related to altering gene expression in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

6. 发明授权

US06617498B1 Inducible promoters 失效
标题翻译：诱导型启动子
公开(公告)号：US06617498B1
公开(公告)日：2003-09-09
申请号：US09655024
申请日：2000-09-05
申请人： Wesley B. Bruce , Carl W. Garnaat , Steven W. Ritchie
发明人： Wesley B. Bruce , Carl W. Garnaat , Steven W. Ritchie
IPC分类号： A01H500
CPC分类号： C12N15/8237 , C12N15/8238 , C12N15/8279
摘要： The present invention provides compositions and methods for regulating the expression of nucleotide sequence of interest in a plant. Compositions include a novel nucleotide sequence comprising an inducible promoter for the 5C9 gene, which encodes a maize patatin-like polypeptide. A method for expressing a nucleotide sequence of interest in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the inducible promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence in an inducible manner.
摘要翻译：本发明提供用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。组合物包括一个新的核苷酸序列，其包含5C9基因的诱导型启动子，其编码玉米patatin样多肽。提供了使用本文公开的启动子序列在植物中表达目的核苷酸序列的方法。该方法包括将可操作地连接到本发明的诱导型启动子的核苷酸序列稳定地并入植物细胞的基因组中，并以可诱导的方式再生表达核苷酸序列的稳定转化的植物。

7. 发明授权

US5882668A Maize protein for insect control 失效
标题翻译：玉米蛋白为昆虫控制
公开(公告)号：US5882668A
公开(公告)日：1999-03-16
申请号：US756855
申请日：1996-11-26
申请人： Carl W. Garnaat , Terry EuClaire Meyer
发明人： Carl W. Garnaat , Terry EuClaire Meyer
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , A01N25/00
CPC分类号： C12N15/8286 , A01N65/00 , A01N65/44 , C07K14/415
摘要： Nucleotide sequences encoding a maize 5C9 polypeptide, the 5C9 polypeptide and structural and functional equivalents, and methods for controlling insect infestation in plants are provided.
摘要翻译：提供编码玉米5C9多肽的核苷酸序列，5C9多肽及其结构和功能等同物，以及控制植物昆虫侵袭的方法。

8. 发明申请

US20100186122A1 AUXOTROPHIC AGROBACTERIUM FOR PLANT TRANSFORMATION AND METHODS THEREOF 失效
标题翻译：用于植物转化的辅助植物及其方法
公开(公告)号：US20100186122A1
公开(公告)日：2010-07-22
申请号：US12650106
申请日：2009-12-30
申请人： JEROME P. RANCH , MATTHIAS LIEBERGESELL , CARL W. GARNAAT , GARY A. HUFFMAN
发明人： JEROME P. RANCH , MATTHIAS LIEBERGESELL , CARL W. GARNAAT , GARY A. HUFFMAN
IPC分类号： A01H1/04 , A01H5/00 , C12N1/21 , C12N15/74
CPC分类号： C12N15/8205 , C12N9/1007
摘要： Auxotrophic Agrobacterium and methods employing auxotrophic Agrobacterium are provided. Auxotrophic Agrobacterium may be used in a variety of methods including biologically containing Agrobacterium comprising a transgene and transforming a plant cell without using an Agrobacterium counter-selective agent. Transforming maize immature embryos using an Agrobacterium auxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic Agrobacterium. Methods for producing an Agrobacterium thymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.
摘要翻译：营养缺陷型农杆菌和使用营养缺陷型农杆菌的方法。营养不良农杆菌可以以多种方法使用，包括生物地含有转基因的农杆菌，并且不使用农杆菌反选择剂来转化植物细胞。使用农杆菌营养缺陷型用于胸苷转化玉米未成熟胚导致与使用原养性农杆菌的转化相当的转化效率。公开了用于生产农杆菌营养缺陷型农杆菌和在转化方法中使用营养缺陷型的方法。还提供了使用本发明的方法生产的转化的组织和植物。

9. 发明授权

US07205455B2 Ecdysone receptors and methods for their use 有权
公开(公告)号：US07205455B2
公开(公告)日：2007-04-17
申请号：US10295370
申请日：2002-11-15
申请人： Marc C. Albertsen , Catherine D. Brooke , Carl W. Garnaat , Bradley Allen Roth
发明人： Marc C. Albertsen , Catherine D. Brooke , Carl W. Garnaat , Bradley Allen Roth
IPC分类号： C12N15/82 , C12N15/90 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8287 , C07K14/43577 , C07K14/721 , C07K2319/00
摘要： The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone or ultraspiracle receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor and a novel ultraspiracle receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.

10. 发明授权

US07151168B2 Ecdysone receptors and methods for their use 有权
标题翻译：蜕皮激素受体及其使用方法
公开(公告)号：US07151168B2
公开(公告)日：2006-12-19
申请号：US10292324
申请日：2002-11-12
申请人： Marc C. Albertsen , Catherine D. Brooke , Carl W. Garnaat , Bradley Allen Roth
发明人： Marc C. Albertsen , Catherine D. Brooke , Carl W. Garnaat , Bradley Allen Roth
IPC分类号： C07H21/00 , C12N15/82
CPC分类号： C12N15/8287 , C07K14/43577 , C07K14/721 , C07K2319/00
摘要： The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone or ultraspiracle receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor and a novel ultraspiracle receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.
摘要翻译：本发明的领域是可诱导的基因表达系统，其中表达由新的蜕皮激素或超尖锐体受体或其衍生物控制。该领域特别涉及用于新型蜕皮激素受体和新型超急性受体的核酸和多肽的分离和表征。核酸和多肽可用于可用蜕皮激素或衍生物受体激动剂诱导的新基因表达系统。

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