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1. 发明申请

US20150218626A1 MULTIPLEX NUCLEIC ACID ANALYSIS 审中-公开
标题翻译：多重核酸分析
公开(公告)号：US20150218626A1
公开(公告)日：2015-08-06
申请号：US14426137
申请日：2012-09-10
申请人： Zhengwen Jiang , Feng Yu , Caihua Li
发明人： Zhengwen Jiang , Feng Yu , Caihua Li
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q1/6862 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2525/204 , C12Q2537/143 , C12Q2561/125 , C12Q2535/125 , C12Q2525/155 , C12Q2537/16 , C12Q2563/107 , C12Q2565/125
摘要： Here provided is a method for multiplex nucleic acid analysis. The method includes steps of hybridizing sets of probes to target nucleic acids in a sample, ligating the hybridized probes, amplifying the ligated probes, and assaying the amplification products to determining the presence, absence, or quantity of the target nucleic acids in the sample. The multiplexity is made available in part by adding detectable moieties and inserting stuffer sequences in primers so that amplification products may be identified on the basis of the detectable moieties and fragment sizes. Also provided is a sensitive method of detecting small copy number changes by measuring the copy number of a plurality of target sites in the nucleic acid in a test sample in comparison to a control sample and then determining the copy number of the nucleic acid based on the measured copy number of the plurality of target sites. Further provided is a kit for multiplex nucleic acid analysis and for small copy number change determination.
摘要翻译：这里提供了多重核酸分析的方法。该方法包括将探针组与样品中的靶核酸杂交，连接杂交探针，扩增连接的探针和测定扩增产物以确定样品中靶核酸的存在，不存在或数量的步骤。通过加入可检测的部分并在引物中插入填充序列，可以部分地获得多重性，从而可以基于可检测部分和片段大小来鉴定扩增产物。还提供了通过与对照样品相比测量测试样品中的核酸中的多个靶位点的拷贝数，然后基于对照样品确定核酸拷贝数，来检测小拷贝数变化的敏感方法测量多个目标站点的拷贝数。还提供了用于多重核酸分析和小拷贝数变化测定的试剂盒。

2. 发明授权

US10059983B2 Multiplex nucleic acid analysis 有权
公开(公告)号：US10059983B2
公开(公告)日：2018-08-28
申请号：US14426137
申请日：2012-09-10
申请人： Zhengwen Jiang , Feng Yu , Caihua Li
发明人： Zhengwen Jiang , Feng Yu , Caihua Li
IPC分类号： C12Q1/68 , C12Q1/6827 , C12Q1/6858 , C12Q1/6883 , C12Q1/6862
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q1/6862 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2525/204 , C12Q2537/143 , C12Q2561/125 , C12Q2535/125 , C12Q2525/155 , C12Q2537/16 , C12Q2563/107 , C12Q2565/125
摘要： Here provided is a method for multiplex nucleic acid analysis. The method includes steps of hybridizing sets of probes to target nucleic acids in a sample, ligating the hybridized probes, amplifying the ligated probes, and assaying the amplification products to determining the presence, absence, or quantity of the target nucleic acids in the sample. The multiplexity is made available in part by adding detectable moieties and inserting stuffer sequences in primers so that amplification products may be identified on the basis of the detectable moieties and fragment sizes. Also provided is a sensitive method of detecting small copy number changes by measuring the copy number of a plurality of target sites in the nucleic acid in a test sample in comparison to a control sample and then determining the copy number of the nucleic acid based on the measured copy number of the plurality of target sites. Further provided is a kit for multiplex nucleic acid analysis and for small copy number change determination.

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