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1. 发明申请

US20070240241A1 METHOD OF PREDICTING A TRAIT OF INTEREST 审中-公开
标题翻译：预测利益的方法
公开(公告)号：US20070240241A1
公开(公告)日：2007-10-11
申请号：US11697581
申请日：2007-04-06
申请人： Maria Ubach , Luis Jurado , Dutt Vinjamoori , Pradip Das , Bradley Krohn , Steven Modiano
发明人： Maria Ubach , Luis Jurado , Dutt Vinjamoori , Pradip Das , Bradley Krohn , Steven Modiano
IPC分类号： A01H1/00 , G01N1/30
CPC分类号： G01N33/5097 , G01N1/30 , G01N21/6458 , G01N33/0098 , G01N33/50 , G01N2400/00
摘要： A method of predicting a trait of interest from a plant by measuring the degree of starch-protein association in the plant is provided. The degree of starch-protein association can be determined by identifying either chemical properties or physical properties, or both chemical and physical properties of plant cells. In a particular embodiment, the degree of starch-protein association is determined by identifying the protein composition of the plant.
摘要翻译：提供了通过测量植物中淀粉 - 蛋白质缔合程度来预测来自植物的性状的方法。淀粉 - 蛋白质缔合程度可以通过鉴定植物细胞的化学性质或物理性质，或化学和物理性质来确定。在一个具体实施方案中，通过鉴定植物的蛋白质组成来确定淀粉 - 蛋白质缔合的程度。

2. 发明申请

US20060286654A1 Alcohol production using sonication 审中-公开
标题翻译：酒精生产使用超声处理
公开(公告)号：US20060286654A1
公开(公告)日：2006-12-21
申请号：US11449089
申请日：2006-06-08
申请人： Michael Kinley , Jonathan Snodgrass , Bradley Krohn
发明人： Michael Kinley , Jonathan Snodgrass , Bradley Krohn
IPC分类号： C12P7/06 , C12M3/00
CPC分类号： C12P7/16 , C12M21/12 , C12M35/04 , C12N1/066 , C12N13/00 , C12P7/06 , C12P7/18 , C12P7/24 , C12P7/56 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： A system comprising one or more transducers and an alcohol production facility having a liquid medium processing stream, the alcohol production facility adapted for use with the one or more transducers is provided. In one embodiment, the one or more transducers apply sonication to the liquid medium processing stream in one or more locations. In one embodiment, the alcohol production facility is an ethanol production facility. In one embodiment, the ethanol production facility utilizes a dry grind process, modified dry grind process or wet mill process. The systems of the present invention utilize sonication at the frequencies and intensities required on an industrial scale to reduce the production cost of alcohol, such as ethanol, by improving alcohol yield per bushel, reducing processing times for higher throughput, reducing operating costs, and increasing the marketability of co-products, among other benefits.
摘要翻译：一种包括一个或多个换能器和具有液体介质处理流的醇生产设备的系统，提供了适用于一个或多个换能器的醇生产设备。在一个实施例中，一个或多个换能器在一个或多个位置中对液体介质处理流应用超声处理。在一个实施方案中，醇生产设备是乙醇生产设备。在一个实施方案中，乙醇生产设备利用干式研磨工艺，改性干式研磨方法或湿式粉碎工艺。本发明的系统以工业规模所要求的频率和强度利用超声处理，通过提高每蒲式耳的酒精产率来降低酒精（如乙醇）的生产成本，减少处理时间，提高产量，降低运营成本，并增加共同产品的市场化等等。

3. 发明申请

US20080044891A1 Biomass conversion to alcohol using ultrasonic energy 审中-公开
标题翻译：使用超声能量生物质转化为酒精
公开(公告)号：US20080044891A1
公开(公告)日：2008-02-21
申请号：US11974757
申请日：2007-10-16
申请人： Michael Kinley , Bradley Krohn
发明人： Michael Kinley , Bradley Krohn
IPC分类号： C12M1/00
CPC分类号： C12P7/06 , C12M21/12 , C12M35/04 , C12M45/02 , C12M45/06 , C12M45/20 , Y02E50/17
摘要： A method comprising applying ultrasonic energy to a biomass to alcohol production process is provided. In one embodiment the alcohol production process is an ethanol production process. In one embodiment, the biomass to alcohol process employs ultrasonic energy as the only means of pretreatment. In one embodiment, the biomass to alcohol process additionally employs a concentrated acid hydrolysis pretreatment. In one embodiment, the biomass to alcohol process additionally employs any conventional pretreatment, such as a hydrothermal or chemical pretreatment, followed by an enzymatic hydrolysis step or a simultaneous enzymatic hydrolysis and saccharification step. In one embodiment, the conventional pretreatment is selected from the group consisting of dilute acid hydrolysis, high pressure hot water-based methods, i.e., hydrothermal treatments such as steam explosion and aqueous hot water extraction, reactor systems (e.g., batch, continuous flow, counter-flow, flow-through, and the like), ammonia explosion, ammonia recycled percolation (ARP), lime treatment and a pH-based process.
摘要翻译：提供了一种方法，其包括将超声波能量施加到生物质至醇生产过程。在一个实施方案中，醇生产方法是乙醇生产方法。在一个实施方案中，生物质与醇的方法使用超声波能量作为预处理的唯一手段。在一个实施方案中，生物质与醇的方法另外采用浓缩酸水解预处理。在一个实施方案中，生物质与醇的方法另外使用任何常规的预处理，例如水热或化学预处理，随后是酶水解步骤或同时酶水解和糖化步骤。在一个实施方案中，常规的预处理选自稀酸水解，高压热水基方法，即水热处理如蒸汽爆炸和水热水提取，反应器系统（例如，批次，连续流动，逆流，流通等），氨爆炸，氨循环渗滤（ARP），石灰处理和基于pH的过程。

4. 发明申请

US20050101484A1 Method of imparting disease resistance to plants by reducing polyphenol oxidase activities 有权
标题翻译：通过减少多酚氧化酶活性赋予植物抗病性的方法
公开(公告)号：US20050101484A1
公开(公告)日：2005-05-12
申请号：US10415759
申请日：2001-11-02
申请人： Salim Hakimi , Bradley Krohn , David Stark
发明人： Salim Hakimi , Bradley Krohn , David Stark
IPC分类号： A01N25/00 , C12N9/02 , C12N15/82
CPC分类号： C12N9/0059 , C12N15/8282
摘要： The present invention relates to a method for enhancing a plant's resistance to fungal diseases by reducing expression of polyphenol oxidase (PPO). The present invention also relates to a method for improving a potato plant's anti-bruising trait. By using antisense technology to generate transgenic potato plants with tuber-specific promoters, PPO can be reduced to a level at which the potato tubers sufficiently increases its resistance to fungal disease symptoms including late blight caused by Phytophthora infestans. Also provided are certain novel combinations of promoters and PPO-derived sequences which also provide improvements in tuber bruise susceptibility.
摘要翻译：本发明涉及通过减少多酚氧化酶（PPO）的表达来提高植物对真菌病的抗性的方法。本发明还涉及一种改善马铃薯植物抗瘀斑性状的方法。通过使用反义技术产生具有块茎特异性启动子的转基因马铃薯植物，PPO可以降低到马铃薯块茎充分增加其对真菌病症状的抗性的水平，包括致病疫霉引起的晚疫病。还提供了促进剂和PPO衍生的序列的某些新颖的组合，其也提供块茎瘀伤敏感性的改善。

5. 发明申请

US20050136520A1 Biomass conversion to alcohol using ultrasonic energy 失效
标题翻译：使用超声能量生物质转化为酒精
公开(公告)号：US20050136520A1
公开(公告)日：2005-06-23
申请号：US10954657
申请日：2004-09-30
申请人： Michael Kinley , Bradley Krohn
发明人： Michael Kinley , Bradley Krohn
IPC分类号： C12M1/42 , C12P7/02 , C12P7/06
CPC分类号： C12P7/06 , C12M21/12 , C12M35/04 , C12M45/02 , C12M45/06 , C12M45/20 , Y02E50/17
摘要： A method comprising applying ultrasonic energy to a biomass to alcohol production process is provided. In one embodiment the alcohol production process is an ethanol production process. In one embodiment, the biomass to alcohol process employs ultrasonic energy as the only means of pretreatment. In one embodiment, the biomass to alcohol process additionally employs a concentrated acid hydrolysis pretreatment. In one embodiment, the biomass to alcohol process additionally employs any conventional pretreatment, such as a hydrothermal or chemical pretreatment, followed by an enzymatic hydrolysis step or a simultaneous enzymatic hydrolysis and saccharification step. In one embodiment, the conventional pretreatment is selected from the group consisting of dilute acid hydrolysis, high pressure hot water-based methods, i.e., hydrothermal treatments such as steam explosion and aqueous hot water extraction, reactor systems (e.g., batch, continuous flow, counter-flow, flow-through, and the like), ammonia explosion, ammonia recycled percolation (ARP), lime treatment and a pH-based process.
摘要翻译：提供了一种方法，其包括将超声波能量施加到生物质至醇生产过程。在一个实施方案中，醇生产方法是乙醇生产方法。在一个实施方案中，生物质与醇的方法使用超声波能量作为预处理的唯一手段。在一个实施方案中，生物质与醇的方法另外采用浓缩酸水解预处理。在一个实施方案中，生物质与醇的方法另外使用任何常规的预处理，例如水热或化学预处理，随后是酶水解步骤或同时酶水解和糖化步骤。在一个实施方案中，常规的预处理选自稀酸水解，高压热水基方法，即水热处理如蒸汽爆炸和水热水提取，反应器系统（例如，批次，连续流动，逆流，流通等），氨爆炸，氨循环渗滤（ARP），石灰处理和基于pH的过程。

6. 发明授权

US07514246B2 Methods for increasing starch levels using sonication 失效
标题翻译：使用超声处理提高淀粉水平的方法
公开(公告)号：US07514246B2
公开(公告)日：2009-04-07
申请号：US11874617
申请日：2007-10-18
申请人： Michael T. Kinley , Jonathan D. Snodgrass , Bradley Krohn
发明人： Michael T. Kinley , Jonathan D. Snodgrass , Bradley Krohn
IPC分类号： C12P7/10
CPC分类号： C12P7/16 , C12M21/12 , C12M35/04 , C12N1/066 , C12N13/00 , C12P7/06 , C12P7/18 , C12P7/24 , C12P7/56 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： A system comprising one or more transducers and an alcohol production facility having a liquid medium processing stream, the alcohol production facility adapted for use with the one or more transducers is provided. In one embodiment, the one or more transducers apply sonication to the liquid medium processing stream in one or more locations. In one embodiment, the alcohol production facility is an ethanol production facility. In one embodiment, the ethanol production facility utilizes a dry grind process, modified dry grind process or wet mill process. The systems of the present invention utilize sonication at the frequencies and intensities required on an industrial scale to reduce the production cost of alcohol, such as ethanol, by improving alcohol yield per bushel, reducing processing times for higher throughput, reducing operating costs, and increasing the marketability of co-products, among other benefits.
摘要翻译：一种包括一个或多个换能器和具有液体介质处理流的醇生产设备的系统，提供了适用于一个或多个换能器的醇生产设备。在一个实施例中，一个或多个换能器在一个或多个位置中对液体介质处理流应用超声处理。在一个实施方案中，醇生产设备是乙醇生产设备。在一个实施方案中，乙醇生产设备利用干式研磨工艺，改性干式研磨方法或湿式粉碎工艺。本发明的系统以工业规模所要求的频率和强度利用超声处理，通过提高每蒲式耳的酒精产率来降低酒精（如乙醇）的生产成本，减少加工时间，提高产量，降低运营成本，并增加共同产品的市场化等等。

7. 发明授权

US07504245B2 Biomass conversion to alcohol using ultrasonic energy 失效
标题翻译：使用超声能量生物质转化为酒精
公开(公告)号：US07504245B2
公开(公告)日：2009-03-17
申请号：US10954657
申请日：2004-09-30
申请人： Michael T. Kinley , Bradley Krohn
发明人： Michael T. Kinley , Bradley Krohn
IPC分类号： C12P7/06
CPC分类号： C12P7/06 , C12M21/12 , C12M35/04 , C12M45/02 , C12M45/06 , C12M45/20 , Y02E50/17
摘要： A method comprising applying ultrasonic energy to a biomass to alcohol production process is provided. In one embodiment the alcohol production process is an ethanol production process. In one embodiment, the biomass to alcohol process employs ultrasonic energy as the only means of pretreatment. In one embodiment, the biomass to alcohol process additionally employs a concentrated acid hydrolysis pretreatment. In one embodiment, the biomass to alcohol process additionally employs any conventional pretreatment, such as a hydrothermal or chemical pretreatment, followed by an enzymatic hydrolysis step or a simultaneous enzymatic hydrolysis and saccharification step. In one embodiment, the conventional pretreatment is selected from the group consisting of dilute acid hydrolysis, high pressure hot water-based methods, i.e., hydrothermal treatments such as steam explosion and aqueous hot water extraction, reactor systems (e.g., batch, continuous flow, counter-flow, flow-through, and the like), ammonia explosion, ammonia recycled percolation (ARP), lime treatment and a pH-based process.
摘要翻译：提供了一种方法，其包括将超声波能量施加到生物质至醇生产过程。在一个实施方案中，醇生产方法是乙醇生产方法。在一个实施方案中，生物质与醇的方法使用超声波能量作为预处理的唯一手段。在一个实施方案中，生物质与醇的方法另外采用浓缩酸水解预处理。在一个实施方案中，生物质与醇的方法另外使用任何常规的预处理，例如水热或化学预处理，随后是酶水解步骤或同时酶水解和糖化步骤。在一个实施方案中，常规的预处理选自稀酸水解，高压热水基方法，即水热处理如蒸汽爆炸和水热水提取，反应器系统（例如，批次，连续流动，逆流，流通等），氨爆炸，氨循环渗滤（ARP），石灰处理和基于pH的过程。

8. 发明申请

US20080096261A1 ALCOHOL PRODUCTION USING SONICATION 失效
标题翻译：使用声音的酒精生产
公开(公告)号：US20080096261A1
公开(公告)日：2008-04-24
申请号：US11874617
申请日：2007-10-18
申请人： Michael Kinley , Jonathan Snodgrass , Bradley Krohn
发明人： Michael Kinley , Jonathan Snodgrass , Bradley Krohn
IPC分类号： C12P7/10
CPC分类号： C12P7/16 , C12M21/12 , C12M35/04 , C12N1/066 , C12N13/00 , C12P7/06 , C12P7/18 , C12P7/24 , C12P7/56 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： A system comprising one or more transducers and an alcohol production facility having a liquid medium processing stream, the alcohol production facility adapted for use with the one or more transducers is provided. In one embodiment, the one or more transducers apply sonication to the liquid medium processing stream in one or more locations. In one embodiment, the alcohol production facility is an ethanol production facility. In one embodiment, the ethanol production facility utilizes a dry grind process, modified dry grind process or wet mill process. The systems of the present invention utilize sonication at the frequencies and intensities required on an industrial scale to reduce the production cost of alcohol, such as ethanol, by improving alcohol yield per bushel, reducing processing times for higher throughput, reducing operating costs, and increasing the marketability of co-products, among other benefits.
摘要翻译：一种包括一个或多个换能器和具有液体介质处理流的醇生产设备的系统，提供了适用于一个或多个换能器的醇生产设备。在一个实施例中，一个或多个换能器在一个或多个位置中对液体介质处理流应用超声处理。在一个实施方案中，醇生产设备是乙醇生产设备。在一个实施方案中，乙醇生产设备利用干式研磨工艺，改性干式研磨方法或湿式粉碎工艺。本发明的系统以工业规模所要求的频率和强度利用超声处理，通过提高每蒲式耳的酒精产率来降低酒精（如乙醇）的生产成本，减少处理时间，提高产量，降低运营成本，并增加共同产品的市场化等等。

9. 发明授权

US07101691B2 Alcohol production using sonication 失效
标题翻译：酒精生产使用超声处理
公开(公告)号：US07101691B2
公开(公告)日：2006-09-05
申请号：US10926783
申请日：2004-08-26
申请人： Michael T. Kinley , Jonathan D. Snodgrass , Bradley Krohn
发明人： Michael T. Kinley , Jonathan D. Snodgrass , Bradley Krohn
IPC分类号： C12P7/06
CPC分类号： C12P7/16 , C12M21/12 , C12M35/04 , C12N1/066 , C12N13/00 , C12P7/06 , C12P7/18 , C12P7/24 , C12P7/56 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： A method comprising applying sonication to a grain-based liquid medium processing stream of a starch-to-alcohol production process with one or more transducers is provided. In one embodiment, the one or more transducers apply sonication to the grain-based liquid medium processing stream in one or more locations. In one embodiment, the starch-to-alcohol production process is a starch-to-ethanol production process. In one embodiment, the starch-to-ethanol production process is a dry grind process, modified dry grind process or wet mill process. The methods of the present invention utilize sonication at the frequencies and intensities required on an industrial scale to reduce the production cost of alcohol, such as ethanol, by improving alcohol yield per bushel, reducing processing times for higher throughput, reducing operating costs, and increasing the marketability of co-products, among other benefits.
摘要翻译：提供了一种方法，其包括使用一种或多种换能器将淀粉 - 醇生产过程的超声处理应用于谷物基液体介质处理流。在一个实施例中，一个或多个换能器在一个或多个位置中对基于谷物的液体介质处理流进行超声处理。在一个实施方案中，淀粉与醇的生产方法是淀粉与乙醇的生产方法。在一个实施方案中，淀粉 - 乙醇生产方法是干式研磨方法，改性干法研磨方法或湿法研磨方法。本发明的方法利用工业规模所要求的频率和强度的超声处理，通过提高每蒲式醇的酒精产率来降低酒精（如乙醇）的生产成本，减少加工时间，提高产量，降低运行成本，并增加共同产品的市场化等等。

10. 发明申请

US20050118692A1 Alcohol production using sonication 失效
标题翻译：酒精生产使用超声处理
公开(公告)号：US20050118692A1
公开(公告)日：2005-06-02
申请号：US10926783
申请日：2004-08-26
申请人： Michael Kinley , Jonathan Snodgrass , Bradley Krohn
发明人： Michael Kinley , Jonathan Snodgrass , Bradley Krohn
IPC分类号： A23K10/38 , C12M1/42 , C12N1/06 , C12N13/00 , C12P7/06 , C12C11/00
CPC分类号： C12P7/16 , C12M21/12 , C12M35/04 , C12N1/066 , C12N13/00 , C12P7/06 , C12P7/18 , C12P7/24 , C12P7/56 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： A system and method comprising one or more transducers and an alcohol production facility having a liquid medium processing stream, the alcohol production facility adapted for use with the one or more transducers is provided. In one embodiment, the one or more transducers apply sonication to the liquid medium processing stream in one or more locations. In one embodiment, the alcohol production facility is an ethanol production facility. In one embodiment, the ethanol production facility utilizes a dry grind process, modified dry grind process or wet milling process. The systems and methods of the present invention utilize sonication at the frequencies and intensities required on an industrial scale to reduce the production cost of alcohol, such as ethanol, by improving alcohol yield per bushel, reducing processing times for higher throughput, reducing operating costs, and increasing the marketability of co-products, among other benefits.
摘要翻译：提供了一种包括一个或多个换能器和具有液体介质处理流的醇生产设备的系统和方法，所述醇生产设备适用于与所述一个或多个换能器一起使用。在一个实施例中，一个或多个换能器在一个或多个位置中对液体介质处理流应用超声处理。在一个实施方案中，醇生产设备是乙醇生产设备。在一个实施方案中，乙醇生产设备利用干式研磨法，改性干法研磨法或湿法研磨法。本发明的系统和方法以工业规模所需的频率和强度利用超声处理，通过提高每蒲式耳的酒精产率来降低酒精如乙醇的生产成本，减少加工时间，提高产量，降低运行成本，并提高联产品的销售能力等优点。

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