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1. 发明授权

US4720454A Genetic probe used in the detection of adrenal hyperplasia 失效
标题翻译：用于检测肾上腺增生的遗传探针
公开(公告)号：US4720454A
公开(公告)日：1988-01-19
申请号：US601650
申请日：1984-04-18
申请人： Perrin C. White , Bo Dupont , Maria I. New
发明人： Perrin C. White , Bo Dupont , Maria I. New
IPC分类号： C12N9/02 , C12Q1/68 , C12N15/00
CPC分类号： C12N9/0087 , C12Q1/6883 , C12Q2600/156 , C12Q2600/172
摘要： A cDNA clone is isolated encoding a bovine adrenal cytochrome P-450 specific for steroid 21-hydroxylation (P-450.sub.C21). This plasmid pC21a contains an insert of 520 base pairs. It hybridizes with mRNA encoding P-450.sub.C21. The peptide encoded by the insert is highly homologous to two peptides isolated from porcine P-450.sub.C21 and shows limited homology to the P-450 induced by phenobarbital in rat liver. This probe and its human equivalents can be used to screen in utero i.e. pre-natal as well as post-natal patients for adrenal hyperplasia since it reacts well with human DNA as shown. These probes can also be useful for an industrial fermentation process to produce cortisone in quantity.
摘要翻译：分离cDNA克隆，其编码对类固醇21-羟基化（P-450C21）特异性的牛肾上腺细胞色素P-450。该质粒pC21a含有520个碱基对的插入片段。它与编码P-450C21的mRNA杂交。由插入片段编码的肽与从猪P-450C21分离的两种肽高度同源，并且与大鼠肝脏中苯巴比妥诱导的P-450显示有限的同源性。该探针及其人类等同物可用于筛选子宫内，即出生前以及出生后患者的肾上腺增生，因为如图所示，它与人类DNA发生反应良好。这些探针也可用于工业发酵过程以量产生可的松。

2. 发明授权

US4710457A Monoclonal antibody for human hematopoietic glycoproteins and method 失效
标题翻译：人类造血糖蛋白单克隆抗体及方法
公开(公告)号：US4710457A
公开(公告)日：1987-12-01
申请号：US509201
申请日：1983-06-29
申请人： Bo Dupont , Yasuo Morishima , Nancy Collins , Shun-ichiro Ogata , Kenneth O. Lloyd
发明人： Bo Dupont , Yasuo Morishima , Nancy Collins , Shun-ichiro Ogata , Kenneth O. Lloyd
IPC分类号： A61K38/00 , C07K16/28 , C07K16/30 , G01N33/53 , C12P37/00 , G01N33/577
CPC分类号： C07K16/289 , C07K16/3061 , A61K38/00 , Y10S435/948
摘要： This invention concerns a new monoclonal antibody (mAb) 4C, recognizing a specific antigen, Leu 200, found in human hematopoietic tissues. The monoclonal antibody precipitates a series of glycoproteins (Leu 200) with a molecular weight range of about 190,000 to 230,000 from both T- and B-cell lines. The series of glycoproteins resolves into four discrete glycoprotein bands, the distribution of which varies according to the cell lines. Bands 3 and 4 predominate in a majority of T-cells whereas band 2 predominates in B cells. Thus, a Leu 200 antigen subset distribution is possible with mAb 4C. Bands 1, 2, 3, 4 have apparent molecular weights of 230K, 215K, 205K, and 190K respectively, with differences in their carbohydrate moieties. mAb 4C is a IgG sub two a, kappa immunoglobulin. 4C has potential use in leukemia, hematopoietic cell differentiation and transplantation diagnoses and therapy.
摘要翻译：本发明涉及识别人造血组织中发现的特异性抗原Leu 200的新单克隆抗体（mAb）4C。单克隆抗体从T细胞和B细胞系中析出一系列分子量范围为约19万至23万的糖蛋白（Leu 200）。糖蛋白系列分解成四个离散的糖蛋白条带，其分布根据细胞系而变化。频带3和4在大多数T细胞中占主导地位，而带2在B细胞中占主导地位。因此，可以用mAb 4C进行Leu 200抗原亚群分布。谱带1,2,3,4分别具有230K，215K，205K和190K的表观分子量，其碳水化合物部分不同。 mAb 4C是一种IgG亚型二，κ免疫球蛋白。 4C可用于白血病，造血细胞分化和移植诊断和治疗。

3. 发明授权

US4677056A Monoclonal antibody subsetting human helper and killer T-cells and method 失效
标题翻译：单克隆抗体亚型人类辅助和杀伤T细胞和方法
公开(公告)号：US4677056A
公开(公告)日：1987-06-30
申请号：US524293
申请日：1983-08-18
申请人： Bo Dupont , Michael K. Hoffman , Nancy Collins , Soo Y. Yang , Yasuo Morishima , Masahide Kobayashi
发明人： Bo Dupont , Michael K. Hoffman , Nancy Collins , Soo Y. Yang , Yasuo Morishima , Masahide Kobayashi
IPC分类号： C07K16/28 , G01N33/50 , C07K15/06 , C12N5/00 , C12P21/00
CPC分类号： C07K16/28 , C07K2317/74 , Y10S435/948
摘要： A monoclonal antibody 4A produced by a human-mouse hybridoma cell line is described. In the presence of complement, 4A subsets both cytotoxic and helper T cells creating a diagnostic tool in blood biochemistry.
摘要翻译：描述了由人 - 小鼠杂交瘤细胞系产生的单克隆抗体4A。在补体的存在下，4A亚细胞毒素和辅助T细胞在血液生物化学中产生诊断工具。

4. 发明授权

US4892828A Monoclonal antibody for human hematopoietic glycoproteins and method 失效
标题翻译：人类造血糖蛋白单克隆抗体及方法
公开(公告)号：US4892828A
公开(公告)日：1990-01-09
申请号：US120608
申请日：1987-11-16
申请人： Bo Dupont , Yasuo Morishima , Nancy Collins , Shun-ichiro Ogata , Kenneth O. Lloyd
发明人： Bo Dupont , Yasuo Morishima , Nancy Collins , Shun-ichiro Ogata , Kenneth O. Lloyd
IPC分类号： A61K38/00 , C07K16/28 , C07K16/30 , G01N33/68
CPC分类号： C07K16/289 , C07K16/3061 , G01N33/68 , A61K38/00
摘要： This invention concerns a new monoclonal antibody (mAb) 4C, recognizing a specific antigen, Leu 200, found in human hematopoietic tissues. The monoclonal antibody precipitates a series of glycoproteins (Leu 200) with a molecular weight range of about 190,000 to 230,000 from both T- and B-cell lines. The series of glycoproteins resolves into four discrete glycoprotein bands, the distribution of which varies according to the cell lines. Bands 3 and 4 predominate in a majority of T-cells whereas band 2 predominates in B cells. Thus, a Leu 200 antigen subset distribution is possible with mAb 4C. Bands 1, 2, 3, 4 have apparent molecular weights of 230K, 215K, 205K, and 190K respectively, with differences in their carbohydrate moieties. nAb 4C is a IgG sub two a,kappa immunoglobulin. 4C has potential use in leukemia, hematopoietic cell differentiation and transplantation diagnoses and therapy.
摘要翻译：本发明涉及识别人造血组织中发现的特异性抗原Leu 200的新单克隆抗体（mAb）4C。单克隆抗体从T细胞和B细胞系中析出一系列分子量范围为约19万至23万的糖蛋白（Leu 200）。糖蛋白系列分解成四个离散的糖蛋白条带，其分布根据细胞系而变化。频带3和4在大多数T细胞中占主导地位，而带2在B细胞中占主导地位。因此，可以用mAb 4C进行Leu 200抗原亚群分布。谱带1,2,3,4分别具有230K，215K，205K和190K的表观分子量，其碳水化合物部分不同。 nAb 4C是IgG亚二，κ免疫球蛋白。 4C可用于白血病，造血细胞分化和移植诊断和治疗。

5. 发明授权

US4645738A Method for differential diagnosis of T cell leukemias using monoclonal antibodies 失效
标题翻译：使用单克隆抗体鉴别T细胞白血病的方法
公开(公告)号：US4645738A
公开(公告)日：1987-02-24
申请号：US537977
申请日：1983-09-30
申请人： Robert W. Knowles , Bo Dupont , Kazuyuki Naito , Yasuo Morishima
发明人： Robert W. Knowles , Bo Dupont , Kazuyuki Naito , Yasuo Morishima
IPC分类号： C07K16/30 , G01N33/53 , G01N33/554 , G01N33/577
CPC分类号： C07K16/3061 , Y10S435/96 , Y10S436/811 , Y10S436/813
摘要： Two monoclonal antibodies (3-3 and 3-40) were produced which identify two new leukemia associated antigens. Both antibodies reacted with most cell lines derived from patients with T lymphoblastic leukemia (T-ALL), but were not detected on suspensions of normal hematopoietic cells, including thymocytes, by cytotoxicity, absorption of indirect immunofluorescence assays. Analysis of fresh leukemic cells indicated that mAb 3-3 only reacted with T-ALL cells, while mAb 3-40 reacted with some non-T non-B ALL cells and a few acute myelocytic leukemia (AML) cells, as well as T-ALL cells. The 3-40 antigen was also found histopathologically in frozen sections of several normal tissues, including the epithelial cells and a few lymphoid cells of the thymus, and some malignant tissues, while the 3-3 antigen was not found in any tissue studied. A "double absorption" assay provided additional serological evidence the the two antibodies identify different antigenic determinants. Biochemical analysis indicated that the molecules immunoprecipitated by mAb 3-3 and mAb 3-40 have molecular weights of 35-40,000 daltons. It is demonstrated that the 3-3 and 3-40 antigens are markers for human T-ALL and can be used along with the normal T lymphocyte antigen, 3A1, to discriminate T-ALL from cutaneous T-cell lymphoma (CTCL), adult T cell leukemia (ATL) and T cell chronic lymphocytic leukemia (T-CLL).
摘要翻译：产生两种单克隆抗体（3-3和3-40），其识别两种新的白血病相关抗原。两种抗体与来自T淋巴细胞白血病患者（T-ALL）的大多数细胞系反应，但在正常造血细胞（包括胸腺细胞）的悬浮液中，通过细胞毒性，间接免疫荧光测定的吸收未检测到。新鲜白血病细胞的分析表明mAb 3-3仅与T-ALL细胞反应，而mAb 3-40与一些非T非B ALL细胞和少数急性骨髓性白血病（AML）细胞以及T -ALL单元格。也在组织病理学上发现3-40抗原在几种正常组织的冷冻切片中，包括上皮细胞和胸腺的少数淋巴样细胞和一些恶性组织，而在任何研究的组织中没有发现3-3抗原。 “双重吸收”测定提供了另外的血清学证据，两种抗体鉴定不同的抗原决定簇。生化分析表明，通过mAb 3-3和mAb 3-40免疫沉淀的分子具有35-40,000道尔顿的分子量。证明3-3和3-40抗原是人T-ALL的标记物，可以与正常T淋巴细胞抗原3A1一起使用，以区分T-ALL与皮肤T细胞淋巴瘤（CTCL），成人 T细胞白血病（ATL）和T细胞慢性淋巴细胞性白血病（T-CLL）。

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