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1. 发明申请

US20160333366A1 Nucleotide Sequence Encoding Homeobox-Leucine Zipper Protein HAT22 (HD-ZIP Protein 22) From Corchorus Olitorius and Corchorus Capsularis and Methods of Use 审中-公开
标题翻译：编码来自Corchorus Olitorius和Corchorus Capsularis的Homeobox-Leucine拉链蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列和使用方法
公开(公告)号：US20160333366A1
公开(公告)日：2016-11-17
申请号：US14781243
申请日：2014-11-21
申请人： Maqsudul ALAM , BANGLADESH JUTE RESEARCH INSTITUTE
发明人： Maqsudul Alam , Mohammed Shahidul Islam , Borhan Ahmed , Mohammed Samiul Haque , Mohammed Monjurul Alarm
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention relates to the isolated polynucleotide encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from the plants Corchorus olitorius and Corchorus capsularis and corresponding polypeptide derived thereof. The present invention also relates to the plants having modulated expression of a nucleic acid encoding a homeobox-leucine zipper HAT22 polypeptide or a homologue thereof, which has the ability to modify, preferably to increase/enhance, the fiber length, plant height, and/or plant biomass. More specifically, the invention relates to polypeptides having homeobox-leucine zipper protein HAT22 activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. The present invention further provides vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22). The invention also provides methods for producing the said protein and methods for modifying the said protein in order to improve their desirable characteristics. The said protein of the invention can be used in a variety of ways, including increasing/enhancing the fiber length, height and biomass of plants and fiber yield.
摘要翻译：本发明涉及编码来自植物Corchorus olitorius和Corchorus capsularis的同源盒 - 亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的分离的多核苷酸及其衍生的相应多肽。本发明还涉及具有调节表达编码同源框 - 亮氨酸拉链HAT22多肽或其同源物的核酸的植物，其具有修饰，优选增加/增强纤维长度，植物高度和/ 或植物生物量。更具体地，本发明涉及具有同源异构体 - 亮氨酸拉链蛋白HAT22活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。本发明还提供载体，表达构建体和宿主细胞，其包含同源异构体 - 亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列和/或组成。本发明还提供了用于生产所述蛋白质的方法和用于改变所述蛋白质的方法，以便改善其所需特征。本发明的所述蛋白质可以以多种方式使用，包括增加/增强植物的纤维长度，高度和生物量以及纤维产量。

2. 发明申请

US20150291937A1 LIGNIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
标题翻译：来自马铃薯多糖的木质素降解酶及其用途
公开(公告)号：US20150291937A1
公开(公告)日：2015-10-15
申请号：US14421739
申请日：2013-08-15
申请人： Bangladesh Jute Research Institute
发明人： Masqsudul Alam , Mohammed S. Islam , Mohammed M. Hossen , Mohammed S. Haque , Mohammed M. Alam
IPC分类号： C12N9/02 , C12N9/08
CPC分类号： C12N9/0061 , C12N9/0065
摘要： The present invention relates to the field of plant breeding and disease resistance in respect of lignin degradation of plant by Macrophomina phaseolina (“M. phaseolina”). The present invention discloses isolated polynucleotide encoding of the lignin degrading enzymes, produced by Macrophomina phaseolina, comprising and consisting of nucleotide sequence set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, 95, 98, 101, 104, 107, 110, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106 and 109 or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, 93, 96, 99, 102, 105, 108 and 111; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, having enhanced production of lignin degrading enzyme. The invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomerase to fermentable sugar in saccharification process of biomass.
摘要翻译：本发明涉及通过Macrophomina phaseolina（“M. phaseolina”）对植物的木质素降解的植物育种和抗病性领域。本发明公开了编码由Macrophomina phaseolina生产的木质素降解酶的分离的多核苷酸，其包含SEQ ID NO：编号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，65，68，71 ，74，77，80，83，86，89，92，95，98，101，104，107，110，1,4，7，10，13，16，19，22，25，28，31，34 ，37，40，43，46，49，52，55，58，61，64，67，70，73，76，79，82，85，88，91，94，97，100，103，106和109 或这些序列的补体。本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60，63，66，69，72，75，78，81，84，87，90，93，96，99，102，105，108及111; 包含该多核苷酸的重组基因构建体; 包含重组基因构建体的转化体和转基因真菌，具有增强木质素降解酶的产生。本发明提供了具有寡聚酶活性的多肽，例如在生物质糖化过程中将顽固性可溶性寡聚酶转化为可发酵糖的酶。

3. 发明授权

US10301639B2 Nucleotide sequence encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from Corchorus olitorius and Corchorus capsularis and methods of use 有权
公开(公告)号：US10301639B2
公开(公告)日：2019-05-28
申请号：US14781243
申请日：2014-11-21
申请人： Bangladesh Jute Research Institute , Rafia Hasina
发明人： Maqsudul Alam , Mohammed Shahidul Islam , Borhan Ahmed , Mohammed Samiul Haque , Mohammed Monjurul Alam
IPC分类号： C12N15/82 , C07K14/415
摘要： The present invention relates to the isolated polynucleotide encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from the plants Corchorus olitorius and Corchorus capsularis and corresponding polypeptide derived thereof. The present invention also relates to the plants having modulated expression of a nucleic acid encoding a homeobox-leucine zipper HAT22 polypeptide or a homologue thereof, which has the ability to modify, preferably to increase/enhance, the fiber length, plant height, and/or plant biomass. More specifically, the invention relates to polypeptides having homeobox-leucine zipper protein HAT22 activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. The present invention further provides vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22). The invention also provides methods for producing the said protein and methods for modifying the said protein in order to improve their desirable characteristics. The said protein of the invention can be used in a variety of ways, including increasing/enhancing the fiber length, height and biomass of plants and fiber yield.

4. 发明申请

US20150291945A1 CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
标题翻译：纤维素和/或致细胞毒素降解大蒜素的酶及其用途
公开(公告)号：US20150291945A1
公开(公告)日：2015-10-15
申请号：US14421774
申请日：2013-08-15
申请人： Bangladesh Jute Research Institute
发明人： Maqsudul Alam , Mohammed S. Islam , Mohammed M. Hossen , Mohammed S. Haque , Mohammed M. Alam
IPC分类号： C12N9/24 , C12N9/38 , C12N9/88 , C12N9/34 , C12N9/42 , C12N9/26
CPC分类号： C12N9/2402 , C12N9/2408 , C12N9/2428 , C12N9/2437 , C12N9/244 , C12N9/2445 , C12N9/2471 , C12N9/2482 , C12N9/2494 , C12N9/88 , C12Y302/01037 , C12Y302/01051 , C12Y302/01055 , C12Y302/01059 , C12Y302/01075 , C12Y302/01089 , C12Y302/0113 , C12Y302/01177
摘要： The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.
摘要翻译：本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina（“M. phaseolina”）的核苷酸序列，包括纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，葡糖苷酶，α-葡糖苷酶，木聚糖酶，甘露聚糖，α-木糖苷酶，α-木糖苷酶，半乳糖苷酶，阿拉伯呋喃糖苷酶，α-岩藻糖苷酶，β-半乳糖苷酶，不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。也提供了包含和/或由该酶基因的核苷酸序列组成的载体，表达构建体和宿主细胞。本发明还提供了用于生产酶的方法和用于改变酶的方法，以便改善其期望的特性。本发明的酶可以多种但不限于制药，农业，食品和饲料加工，生物燃料，能源效率和工业环境。这些酶也可用于将木质纤维素生物质完全水解成单糖，然后将其发酵成液体燃料和化学原料。

5. 发明授权

US09683221B2 Lignin degrading enzymes from macrophomina phaseolina and uses thereof 有权
公开(公告)号：US09683221B2
公开(公告)日：2017-06-20
申请号：US14421739
申请日：2013-08-15
申请人： Bangladesh Jute Research Institute
发明人： Masqsudul Alam , Mohammed S. Islam , Mohammed M. Hossen , Mohammed S. Haque , Mohammed M. Alam
IPC分类号： C12N9/08 , C12N9/02 , C12N1/00 , C12N15/00 , C12P21/06 , C12P19/34 , C07H21/04 , C07K1/00
CPC分类号： C12N9/0061 , C12N9/0065
摘要： The present invention relates to the field of plant breeding and disease resistance in respect of lignin degradation by Macrophomina phaseolina (“M. phaseolina”). Disclosed are isolated polynucleotides encoding the lignin degrading enzymes produced by Macrophomina phaseolina, or the complement of such sequences. Also disclosed are isolated polypeptides encoded by various polynucleotide sequences; a recombinant gene construct comprising a polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, having enhanced production of lignin degrading enzyme. Further disclosed are polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomerase to fermentable sugar in saccharification process of biomass.

6. 发明申请

US20150259667A1 PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
标题翻译：来自大麦酚酞的PECTIN降解酶及其用途
公开(公告)号：US20150259667A1
公开(公告)日：2015-09-17
申请号：US14421729
申请日：2013-08-15
申请人： Bangladesh Jute Research Institute , RAFIA HASINA
发明人： Maqsudul Alam , Mohammed S. Islam , Mohammed M. Hossen , Mohammed S. Haque , Mohammed M. Alam
IPC分类号： C12N9/88 , C12N9/18
CPC分类号： C12N9/88 , C12N9/18 , C12N9/2405 , C12Y301/01011 , C12Y402/02002 , C12Y402/02023
摘要： The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.
摘要翻译：本发明公开了分离的多核苷酸编码酶，该多核苷酸来自真菌巨噬细胞相容素（“M. phaseolina”），其负责降解果胶，并且其包含和/或由SEQ ID NO：1所示的核苷酸序列组成。编号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，1,4,7 ，10,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58和61，或这些序列的补体。本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60及63条; 包含该多核苷酸的重组基因构建体; 转化体和转基因真菌，其包含具有或具有增强的果胶降解酶生产的重组基因构建体。本发明的多肽可以用于制造果汁，纺织品，纸浆和纸，咖啡，茶和油提取和果胶废水处理。

7. 发明授权

US10125371B2 Nucleotide sequence encoding WUSCHEL-related homeobox4 (WOX4) protein from Corchorus olitorius and Corchorus capsularis and methods of use for same 有权
公开(公告)号：US10125371B2
公开(公告)日：2018-11-13
申请号：US14781169
申请日：2014-11-20
申请人： BANGLADESH JUTE RESEARCH INSTITUTE , Rafia Hasina
发明人： Maqsudul Alam , Mohammed Shahidul Islam , Borhan Ahmed , Mohammed Samiul Haque , Mohammed Monjurual Alam
IPC分类号： C12N15/82 , C07K14/415 , A01H5/00
摘要： The present invention discloses isolated polynucleotides encoding WUSCHEL-related homeobox4 proteins from two species of jute plants, namely, the Corchorus olitorius (“C. olitorius”) and Corchorus capsularis (“C. capsularis”), and corresponding polypeptides derived therefrom. The disclosed polynucleotide sequences encode WUSCHEL-related homeobox4 polypeptides (WOX4), which possess catalytic activities in enhancing fiber production in jute. The present invention also relates to the plants having a modulated expression of a nucleic acid encoding a WOX4 polypeptide, which have enhanced fiber yield relative to corresponding wild type plants or other control plants. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the protein are also provided. Also disclosed are methods for producing the proteins and methods for modifying the proteins in order to improve their desirable characteristics. The proteins of the invention can be used in a variety of ways, including inducing, initiating, improving, or enhancing plant growth, plant height, fiber and seed yield.

8. 发明授权

US09765322B2 Pectin degrading enzymes from macrophomina phaseolina and uses thereof 有权
公开(公告)号：US09765322B2
公开(公告)日：2017-09-19
申请号：US14421729
申请日：2013-08-15
申请人： Bangladesh Jute Research Institute , Rafia Hasina
发明人： Maqsudul Alam , Mohammed S. Islam , Mohammed M. Hossen , Mohammed S. Haque , Mohammed M. Alam
IPC分类号： C12N15/60 , C12N9/88 , C12N9/18 , C12N9/24
CPC分类号： C12N9/88 , C12N9/18 , C12N9/2405 , C12Y301/01011 , C12Y402/02002 , C12Y402/02023
摘要： The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.

9. 发明授权

US09765315B2 Cellulose and/or hemicelluloses degrading enzymes from Macrophomina phaseolina and uses thereof 有权
公开(公告)号：US09765315B2
公开(公告)日：2017-09-19
申请号：US14421774
申请日：2013-08-15
申请人： Bangladesh Jute Research Institute
发明人： Maqsudul Alam , Mohammed S. Islam , Mohammed M. Hossen , Mohammed S. Haque , Mohammed M. Alam
IPC分类号： C07H21/02 , C12N9/42 , C12N9/24 , C12N9/26 , C12N9/34 , C12N9/38 , C12N9/88
CPC分类号： C12N9/2402 , C12N9/2408 , C12N9/2428 , C12N9/2437 , C12N9/244 , C12N9/2445 , C12N9/2471 , C12N9/2482 , C12N9/2494 , C12N9/88 , C12Y302/01037 , C12Y302/01051 , C12Y302/01055 , C12Y302/01059 , C12Y302/01075 , C12Y302/01089 , C12Y302/0113 , C12Y302/01177
摘要： The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.

10. 发明申请

US20140344997A1 POLYNUCLEOTIDES ENCODING ENZYMES FROM THE JUTE LIGNIN BIOSYNTHETIC PATHWAY 有权
标题翻译：多核苷酸编码来自JUTE LIGNIN BIOSYNEE PATHWAY的酶
公开(公告)号：US20140344997A1
公开(公告)日：2014-11-20
申请号：US14114623
申请日：2012-04-25
申请人： Bangladesh Jute Research Institute
发明人： Maqsudul Alam , Haseena Khan , Mahboob Zaman , Mohammed K. Uddin , Mohammed S. Haque , Mohammed S. Islam , Muhammed S. Azam
IPC分类号： C12N15/82 , C12N9/02 , C12N9/00 , C12N9/04 , C12N9/10
CPC分类号： C12N15/8255 , C12N9/0006 , C12N9/0008 , C12N9/0073 , C12N9/1007 , C12N9/93 , C12N15/8261 , C12N15/8279 , Y02A40/146
摘要： Disclosed are polynucleotides encoding polypeptides that comprise the biosynthetic pathway for lignin in the jute plant. The present invention relates generally to the field of plant lignin biosynthesis genes, polypeptides encoded by such genes, and the use of such polynucleotide and polypeptide sequences for controlling plant lignin production. Also disclosed are methods for using the polynucleotides and polypeptides to influence the quality and amount of fiber produced by jute.
摘要翻译：公开了编码在黄麻植物中包含木质素生物合成途径的多肽的多核苷酸。本发明一般涉及植物木质素生物合成基因领域，由这些基因编码的多肽，以及这些多核苷酸和多肽序列用于控制植物木质素产生的用途。还公开了使用多核苷酸和多肽来影响由黄麻生产的纤维的质量和量的方法。

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