专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09453233B2 Method for producing Kluyveromyces marxianus transformant 有权
标题翻译：生产马克斯克鲁维酵母转化株的方法
公开(公告)号：US09453233B2
公开(公告)日：2016-09-27
申请号：US13634891
申请日：2011-02-01
申请人： Rinji Akada , Hisashi Hoshida , Babiker Mohamed Ahmed Abdel-Banat , Jun Asakawa
发明人： Rinji Akada , Hisashi Hoshida , Babiker Mohamed Ahmed Abdel-Banat , Jun Asakawa
IPC分类号： C12N15/74 , C12N15/81 , C12N15/64 , C07K14/37 , C12N1/19
CPC分类号： C12N15/815 , C07K14/37 , C12N15/64
摘要： An object to be solved by the present invention is to provide, for example, a method for producing a Kluyveromyces marxianus transformant by a method for conveniently and efficiently connecting the ends of DNA fragments without using specific restriction enzymes or their recognition sequences, and a method for producing a useful substance using the transformant. The present inventors have found, as means to solve the object, a method comprising introducing two or more linear double-stranded DNA fragments free from a Kluyveromyces marxianus autonomously replicating sequence into Kluyveromyces marxianus and selecting a transformant comprising a desired DNA ligation product with marker gene expression by the desired DNA ligation product as an index, or comprising introducing a linear double-stranded DNA fragment comprising a Kluyveromyces marxianus autonomously replicating sequence, alone or in combination with one or more linear double-stranded DNA fragment(s) different therefrom into Kluyveromyces marxianus and selecting a transformant comprising a desired circular DNA ligation product with marker gene expression by the desired circular DNA ligation product as an index.
摘要翻译：本发明要解决的问题是提供例如通过方法有效连接DNA片段末端而不使用特异性限制酶或其识别序列的方法来生产马克斯克鲁维酵母转化体，以及方法用于使用转化体生产有用物质。作为解决目的的手段，本发明人已经发现，将不含马克斯克鲁维酵母自主复制序列的两条或更多条线性双链DNA片段引入马克斯克鲁维酵母（Kluyveromyces marxianus）并选择含有标记基因的所需DNA连接产物的转化体通过所需的DNA连接产物作为指标表达，或包括将包含马克斯克鲁维酵母自主复制序列的线性双链DNA片段单独或与其不同的一个或多个线性双链DNA片段组合引入克鲁维酵母并且通过所需的环状DNA连接产物作为指标选择包含具有标记基因表达的所需环状DNA连接产物的转化体。

2. 发明申请

US20130059389A1 Method for Producing Kluyveromyces Marxianus Transformant 有权
标题翻译：生产马克斯克鲁维酵母转化体的方法
公开(公告)号：US20130059389A1
公开(公告)日：2013-03-07
申请号：US13634891
申请日：2011-02-01
申请人： Rinji Akada , Hisashi Hoshida , Babiker Mohamed Ahmed Abdel-Banat , Jun Asakawa
发明人： Rinji Akada , Hisashi Hoshida , Babiker Mohamed Ahmed Abdel-Banat , Jun Asakawa
IPC分类号： C12N15/81 , C12N15/31
CPC分类号： C12N15/815 , C07K14/37 , C12N15/64
摘要： An object to be solved by the present invention is to provide, for example, a method for producing a Kluyveromyces marxianus transformant by a method for conveniently and efficiently connecting the ends of DNA fragments without using specific restriction enzymes or their recognition sequences, and a method for producing a useful substance using the transformant. The present inventors have found, as means to solve the object, a method comprising introducing two or more linear double-stranded DNA fragments free from a Kluyveromyces marxianus autonomously replicating sequence into Kluyveromyces marxianus and selecting a transformant comprising a desired DNA ligation product with marker gene expression by the desired DNA ligation product as an index, or comprising introducing a linear double-stranded DNA fragment comprising a Kluyveromyces marxianus autonomously replicating sequence, alone or in combination with one or more linear double-stranded DNA fragment(s) different therefrom into Kluyveromyces marxianus and selecting a transformant comprising a desired circular DNA ligation product with marker gene expression by the desired circular DNA ligation product as an index.
摘要翻译：本发明要解决的问题是提供例如通过方法有效连接DNA片段末端而不使用特异性限制酶或其识别序列的方法来生产马克斯克鲁维酵母转化体，以及方法用于使用转化体生产有用物质。作为解决目的的手段，本发明人已经发现，将不含马克斯克鲁维酵母自主复制序列的两条或更多条线性双链DNA片段引入马克斯克鲁维酵母（Kluyveromyces marxianus）并选择含有标记基因的所需DNA连接产物的转化体通过所需的DNA连接产物作为指标表达，或包括将包含马克斯克鲁维酵母自主复制序列的线性双链DNA片段单独或与其不同的一个或多个线性双链DNA片段组合引入克鲁维酵母并且通过所需的环状DNA连接产物作为指标选择包含具有标记基因表达的所需环状DNA连接产物的转化体。

3. 发明授权

US08198089B2 Flocculent yeast and method for production thereof 有权
标题翻译：絮状酵母及其生产方法
公开(公告)号：US08198089B2
公开(公告)日：2012-06-12
申请号：US12922602
申请日：2009-03-18
申请人： Rinji Akada , Sanom Nonklang , Hisashi Hoshida , Babiker Mohamed Ahmed Abdel-Banat
发明人： Rinji Akada , Sanom Nonklang , Hisashi Hoshida , Babiker Mohamed Ahmed Abdel-Banat
IPC分类号： C12N15/70 , C12N1/19 , C12N1/00
CPC分类号： C07K14/395 , C12N15/81 , C12N15/815 , C12P7/06 , Y02E50/17
摘要： It is to provide a novel Kluyveromyces marxianus transformant having thermotolerance and flocculation property, suitable for the industrial production of bioethanol, by introducing a foreign flocculation gene into Kluyveromyces marxianus, and an efficient method for producing the transformant. The present inventors focused on the flocculation gene FLO of Saccharomyces cerevisiae as a foreign gene to confer flocculation property to Kluyveromyces marxianus and produced a linear DNA fragment comprising a known expression promoter sequence and a FLO gene sequence derived from Saccharomyces cerevisiae. As a result of introducing this linear DNA fragment into Kluyveromyces marxianus, the present inventors have confirmed that Kluyveromyces marxianus transformant can be obtained efficiently, and that the flocculation property of the above transformant is unexpectedly and significantly enhanced. The present invention has been thus completed.
摘要翻译：通过将外源絮凝基因引入马克斯克鲁维酵母（Kluyveromyces marxianus）中，提供了具有耐热性和絮凝性的新型克鲁维酵母转化体，适用于生物乙醇的工业化生产，也是生产转化体的有效方法。本发明人着重于酿酒酵母的絮凝基因FLO作为外源基因以赋予马克斯克鲁维酵母的絮凝性，并产生包含已知表达启动子序列和源自酿酒酵母的FLO基因序列的线性DNA片段。作为将线性DNA片段引入马克斯克鲁维酵母的结果，本发明人已经证实，可以有效地获得马克斯克鲁维酵母转化株，并且意外地显着提高了上述转化体的絮凝性。从而完成了本发明。

4. 发明申请

US20110020937A1 FLOCCULENT YEAST AND METHOD FOR PRODUCTION THEREOF 有权
标题翻译：浮游植物及其生产方法
公开(公告)号：US20110020937A1
公开(公告)日：2011-01-27
申请号：US12922602
申请日：2009-03-18
申请人： Rinji Akada , Sanom Nonklang , Hisashi Hoshida , Babiker Mohamed Ahmed Abdel-Banat
发明人： Rinji Akada , Sanom Nonklang , Hisashi Hoshida , Babiker Mohamed Ahmed Abdel-Banat
IPC分类号： C12N15/74 , C12N1/19
CPC分类号： C07K14/395 , C12N15/81 , C12N15/815 , C12P7/06 , Y02E50/17
摘要： It is to provide a novel Kluyveromyces marxianus transformant having thermotolerance and flocculation property, suitable for the industrial production of bioethanol, by introducing a foreign flocculation gene into Kluyveromyces marxianus, and an efficient method for producing the transformant. The present inventors focused on the flocculation gene FLO of Saccharomyces cerevisiae as a foreign gene to confer flocculation property to Kluyveromyces marxianus and produced a linear DNA fragment comprising a known expression promoter sequence and a FLO gene sequence derived from Saccharomyces cerevisiae. As a result of introducing this linear DNA fragment into Kluyveromyces marxianus, the present inventors have confirmed that Kluyveromyces marxianus transformant can be obtained efficiently, and that the flocculation property of the above transformant is unexpectedly and significantly enhanced. The present invention has been thus completed.
摘要翻译：通过将外源絮凝基因引入马克斯克鲁维酵母（Kluyveromyces marxianus）中，提供了具有耐热性和絮凝性的新型克鲁维酵母转化体，适用于生物乙醇的工业化生产，是生产转化体的有效方法。本发明人着重于酿酒酵母的絮凝基因FLO作为外源基因以赋予马克斯克鲁维酵母的絮凝性，并产生包含已知表达启动子序列和源自酿酒酵母的FLO基因序列的线性DNA片段。作为将线性DNA片段引入马克斯克鲁维酵母的结果，本发明人已经证实，可以有效地获得马克斯克鲁维酵母转化株，并且意外地显着提高了上述转化体的絮凝性。从而完成了本发明。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式