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1. 发明申请

US20100240041A1 MICROFLUIDIC DEVICE FOR TRAPPING SINGLE CELL 有权
标题翻译：用于捕获单细胞的微流体装置
公开(公告)号：US20100240041A1
公开(公告)日：2010-09-23
申请号：US12670990
申请日：2008-07-31
申请人： Tadashi Matsunaga , Atsushi Arakaki , Tomoyuki Taguchi , Masahito Hosokawa
发明人： Tadashi Matsunaga , Atsushi Arakaki , Tomoyuki Taguchi , Masahito Hosokawa
IPC分类号： C12Q1/68 , C12M1/00 , C12N5/00
CPC分类号： G01N15/1484 , B01L3/502761 , B01L2200/0668 , B01L2300/0681 , B01L2300/0819 , B01L2300/0877 , B01L2300/0887 , B01L2400/049 , C12Q1/6841 , G01N15/1475 , C12Q2565/629
摘要： It is to provide a microfluidic device which separates and captures with high efficiency a large amount of cells in a sample at one-cell level without damaging the cells by utilizing a microfabrication technology. The device sequentially comprises: an upper substrate consisting of a plastic plate provided with a groove for forming a microchannel communicating a sample-supply opening and a sample-discharge opening; a plastic upper flat plate forming the microchannel by cooperating with the upper substrate, the plate being provided with an aperture for a micromesh; a holding plate holding a plastic micromesh having a plurality of micro through-pores for capturing cells; a plastic lower flat plate provided with an aperture for suctioning; and a lower substrate consisting of a plastic flat plate provided with a groove for forming a suction channel, wherein the micro through-pores for capturing cells of the micromesh have an inverted mortar-like shape or a cylindrical shape, and wherein the upper flat plate is constituted by a softer plastic as compared to the upper substrate.
摘要翻译：它提供了一种微流体装置，其以单细胞水平高效地分离和捕获样品中的大量细胞，而不通过利用微细加工技术损伤细胞。所述装置依次包括：上基板，由具有沟槽的塑料板构成，所述槽用于形成连通供样开口的微通道和采样排出口; 通过与上基板配合形成微通道的塑料上平板，该板设置有微孔的孔; 保持板，其保持具有用于捕获细胞的多个微通孔的塑料微镜; 一个塑料下平板设有一个用于抽吸的孔; 以及由设置有用于形成吸引通道的槽的塑料平板构成的下基板，其中，用于捕获微胶粒的细胞的微通孔具有倒立的砂浆状或圆筒形状，并且其中上平板由与上基板相比较软的塑料构成。

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