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1. 发明授权

US07049486B2 Method of inducing gene expression in plant and the plant treated thereby 失效
标题翻译：诱导植物中基因表达的方法和由此处理的植物
公开(公告)号：US07049486B2
公开(公告)日：2006-05-23
申请号：US10049710
申请日：2001-06-15
申请人： Atsuhiko Shinmyo , Kou Kato , Yasuhiro Yamada , Takuya Nihira , Takuya Shindo
发明人： Atsuhiko Shinmyo , Kou Kato , Yasuhiro Yamada , Takuya Nihira , Takuya Shindo
IPC分类号： C12N15/82 , C12N15/31 , A01H5/00
CPC分类号： C12N15/8238 , C12N15/635 , C12N15/8216 , C12N15/8237 , C12N15/8265 , C12N15/8287
摘要： The present invention is to provide a method which comprises providing a plant with characters of a repressor and operator both constituting a gene expression inducing system with an actinomycete autogenous regulatory factor as an inducer by gene transfer and administering the actinomycete autogenous regulatory factor to the transformed plant to thereby induce the expression of a gene placed under the control of the operator at a site of administration of the actinomycete autogenous regulatory factor. This method makes it possible to cause expression of a desired gene at a desired time and site, thus enabling even the production, in a plant, of a metabolite otherwise disadvantageous to the growth of the plant. It is also useful in preventing transformant plants from spreading through the environment by controlling the fertility thereof.
摘要翻译：本发明提供一种方法，其包括向植物提供具有阻遏物和操作者的特征的两者，所述阻遏物和操纵子均构成基因表达诱导系统，其具有通过基因转移的放线菌自身调节因子作为诱导物，并将放线菌自体调节因子施用于转化植物从而在放线菌自体调节因子的施用部位诱导置于操作者控制下的基因的表达。这种方法使得可以在期望的时间和位点引起所需基因的表达，从而甚至能够在植物中生产代谢物，否则对植物的生长不利。也可以通过控制其生育力来防止转化植物通过环境传播。

2. 发明授权

US07335502B2 Chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase gene 失效
标题翻译：氯醇和羟基羧酸酯不对称水解酶基因
公开(公告)号：US07335502B2
公开(公告)日：2008-02-26
申请号：US11490749
申请日：2006-07-20
申请人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
发明人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
IPC分类号： C12N9/18 , C12N5/00 , C12N1/20 , C12P21/06 , C07H21/04
CPC分类号： C12P41/005 , C07K2319/02 , C12N9/18 , C12P7/42 , C12P7/62 , C12P17/04
摘要： The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ. ID. NO: 1, a gene encoding a protein having an amino acid sequence of SEQ. ID. NO: 2, and E. coli DH5α (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.
摘要翻译：本发明提供具有不对称水解酶活性的基因，其可用于合成光学活性羧酸，其对映体酯和内酯，以及衍生自肠杆菌属的羟基羧酸酯不对称水解酶（EnHCH）。属于肠杆菌属的细菌DS-S-75菌株（FERM BP-5494），由SEQ ID NO：1的碱基序列显示的EnHCH基因。 ID。 NO：1，编码具有SEQ ID NO：1的氨基酸序列的蛋白质的基因。 ID。 NO：2和大肠杆菌DH5α（pKK-EnHCH），保藏在国际专利生物保藏中心，国立先进工业科学技术研究所作为FERM BP-08466。

3. 发明申请

US20060257988A1 Chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase gene 失效
标题翻译：氯醇和羟基羧酸酯不对称水解酶基因
公开(公告)号：US20060257988A1
公开(公告)日：2006-11-16
申请号：US11490749
申请日：2006-07-20
申请人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
发明人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
IPC分类号： C12N9/18
CPC分类号： C12P41/005 , C07K2319/02 , C12N9/18 , C12P7/42 , C12P7/62 , C12P17/04
摘要： The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ. ID. NO: 1, a gene encoding a protein having an amino acid sequence of SEQ. ID. NO: 2, and E. coli DH5α (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.
摘要翻译：本发明提供具有不对称水解酶活性的基因，其可用于合成光学活性羧酸，其对映体酯和内酯，以及衍生自肠杆菌属的羟基羧酸酯不对称水解酶（EnHCH）。属于肠杆菌属的细菌DS-S-75菌株（FERM BP-5494），由SEQ ID NO：1的碱基序列显示的EnHCH基因。 ID。 NO：1，编码具有SEQ ID NO：1的氨基酸序列的蛋白质的基因。 ID。 NO：2和大肠杆菌DH5α（pKK-EnHCH），保藏在国际专利生物保藏中心，国立先进工业科学技术研究所作为FERM BP-08466。

4. 发明授权

US07112428B2 Chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase gene 有权
标题翻译：氯醇和羟基羧酸酯不对称水解酶基因
公开(公告)号：US07112428B2
公开(公告)日：2006-09-26
申请号：US10680024
申请日：2003-10-07
申请人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
发明人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
IPC分类号： C12N9/18 , C12N5/00 , C12N1/20 , C12P21/06 , C12P7/42 , C07H21/04
CPC分类号： C12P41/005 , C07K2319/02 , C12N9/18 , C12P7/42 , C12P7/62 , C12P17/04
摘要： The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ.ID.NO: 1, a gene encoding a protein having an amino acid sequence of SEQ.ID.NO: 2, and E. coli DH5α (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.
摘要翻译：本发明提供具有不对称水解酶活性的基因，其可用于合成光学活性羧酸，其对映体酯和内酯，以及衍生自肠杆菌属的羟基羧酸酯不对称水解酶（EnHCH）。属于肠杆菌属的细菌的DS-S-75菌株（FERM BP-5494），SEQ ID NO：1的碱基序列所示的EnHCH基因，编码具有SEQ ID NO：1的氨基酸序列的蛋白质的基因。 ID.NO:2和大肠杆菌DH5α（pKK-EnHCH），保藏在国际专利生物保藏中心，国立先进工业科学技术研究所作为FERM BP-08466。

5. 发明申请

US20080127373A1 Process for producing a decaffeinated coffee plant by genetic recombination 审中-公开
标题翻译：通过遗传重组生产脱咖啡因咖啡植物的方法
公开(公告)号：US20080127373A1
公开(公告)日：2008-05-29
申请号：US10521244
申请日：2005-01-14
申请人： Shinjiro Ogita , Hiroshi Sano , Nozomu Koizumi , Atsuhiko Shinmyo
发明人： Shinjiro Ogita , Hiroshi Sano , Nozomu Koizumi , Atsuhiko Shinmyo
IPC分类号： A01H5/00
CPC分类号： C12N15/8243 , A01H5/00 , C12N9/1007
摘要： The invention provides a process for producing a decaffeinated coffee plant by genetic recombination comprising a step for preparing an antisense sequence or an RNAi sequence of a gene coding for an enzyme related to the caffeine biosynthetic pathway and constructing an expression vector for transformation, a step for introducing the obtained expression vector into Agrobacterium, a step for infecting a cell division-activated tissue piece of a coffee plant or a callus or an adventitious embryo induced from a tissue piece of a coffee plant with the Agrobacterium to transform the tissue piece, the callus or the adventitious embryo and a step for obtaining an transformed coffee plant from a transformed tissue piece, a transformed callus or a transformed adventitious embryo so that a coffee plant with low caffeine content can be produced by suppressing expression of a gene coding for an enzyme related to the caffeine biosynthetic pathway by the antisense method or the RNAi method.
摘要翻译：本发明提供了一种通过遗传重组生产脱咖啡因咖啡植物的方法，包括制备编码与咖啡因生物合成途径相关的酶的基因的反义序列或RNAi序列的步骤，并构建用于转化的表达载体的步骤将获得的表达载体导入农杆菌中，用于感染咖啡植物或愈伤组织的细胞分裂激活的组织片或由土壤杆菌从咖啡植物的组织块诱导的不定胚以转化组织块，愈伤组织或不定胚，以及从转化的组织片，转化愈伤组织或转化的不定胚获得转化的咖啡植物的步骤，从而可以通过抑制编码相关酶的基因的表达来产生咖啡因含量低的咖啡植物通过反义方法或RNAi方法转化为咖啡因生物合成途径。

6. 发明授权

US06573429B1 DNA fragment for stable expression of an exogenous gene in a plant 失效
标题翻译：用于在植物中稳定表达外源基因的DNA片段
公开(公告)号：US06573429B1
公开(公告)日：2003-06-03
申请号：US09711933
申请日：2000-11-15
申请人： Atsuhiko Shinmyo , Kazuya Yoshida , Ko Kato , Shingo Nagaya , Michio Shibata , Ryutaro Aida
发明人： Atsuhiko Shinmyo , Kazuya Yoshida , Ko Kato , Shingo Nagaya , Michio Shibata , Ryutaro Aida
IPC分类号： A01H100
CPC分类号： C12N15/822
摘要： A method useful for stable expression of an exogenous gene, introduced into a plant, is provided. By introducing an exogenous gene and 5′ upstream sequence of tobacco dehydogenase concurrently, stable expression of the exogenous gene introduced into a plant can be achieved.
摘要翻译：提供了可用于稳定表达引入植物中的外源基因的方法。通过同时引入烟草脱氢酶的外源基因和5'上游序列，可以实现引入植物的外源基因的稳定表达。

7. 发明授权

US06229070B1 Method of stable gene expression in a transgenic plant utilizing an insulator nucleotide sequence from the sea urchin arylsulfatase gene 失效
标题翻译：利用海胆芳基硫酸酯酶基因的绝缘体核苷酸序列，在转基因植物中稳定表达基因的方法
公开(公告)号：US06229070B1
公开(公告)日：2001-05-08
申请号：US09444570
申请日：1999-11-19
申请人： Atsuhiko Shinmyo , Kazuya Yoshida , Ko Kato , Koji Akasaka , Takaaki Kusumi , Yoshikazu Tanaka
发明人： Atsuhiko Shinmyo , Kazuya Yoshida , Ko Kato , Koji Akasaka , Takaaki Kusumi , Yoshikazu Tanaka
IPC分类号： A01H500
CPC分类号： C12N15/822 , C12N15/8216
摘要： A method for the stable expression of an introduced exogenous gene in a plant or plant cell is provided. Stable expression of an exogenous gene that was introduced was achieved by operably linking an upstream sequence of sea urchin arylsulfatase gene as an insulator.
摘要翻译：提供了在植物或植物细胞中稳定表达引入的外源基因的方法。引入的外源基因的稳定表达是通过可靠地连接上游的海胆芳基硫酸酯酶基因序列作为绝缘体来实现的。

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