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1. 发明授权

US08835616B2 Methods for the production and secretion of modified peptides 有权
标题翻译：修饰肽的生产和分泌方法
公开(公告)号：US08835616B2
公开(公告)日：2014-09-16
申请号：US11792516
申请日：2005-12-07
申请人： Gert Nikolaas Moll , Anneke Kuipers , Rick Rink , Arnold Jacob Mathieu Driessen , Oscar Paul Kuipers
发明人： Gert Nikolaas Moll , Anneke Kuipers , Rick Rink , Arnold Jacob Mathieu Driessen , Oscar Paul Kuipers
IPC分类号： C07H21/04 , C12P21/06 , C12N15/62
CPC分类号： C12N15/625
摘要： The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase. Also provided is a method for producing a polypeptide in a host cell, comprising providing a host cell with a nucleic acid construct according to the invention and allowing expression and secretion of the encoded polypeptide by said host cell, wherein said host cell comprises at least a dehydratase capable of modifying said encoded polypeptide and a non-lantibiotic export system capable of secreting said modified polypeptide.
摘要翻译：本发明涉及在重组宿主细胞中产生异源多肽。更具体地，本发明涉及由一种或多种羊毛硫素合成酶修饰的肽（例如生物活性肽）的产生和分泌。本发明提供了编码多肽的核酸构建体，其包含1）非耐药物出口信号，其被非拉丁比亚出口系统识别; 2）由至少一种羊毛硫抗生素脱水酶（例如LanB）和所述出口信号和所述前导肽的C-末端识别的羊毛硫素前导肽，3）感兴趣的肽含有一个或多个丝氨酸或苏氨酸残基，其中可以通过所述脱水酶进行翻译后脱水。还提供了在宿主细胞中产生多肽的方法，其包括向宿主细胞提供根据本发明的核酸构建体，并允许所述宿主细胞表达和分泌编码的多肽，其中所述宿主细胞至少包含能够修饰所述编码的多肽的脱水酶和能够分泌所述修饰的多肽的非拉链菌素出口系统。

2. 发明申请

US20090042246A1 Methods For The Production And Secretion Of Modified Peptides 有权
标题翻译：修饰肽的生产和分泌方法
公开(公告)号：US20090042246A1
公开(公告)日：2009-02-12
申请号：US11792516
申请日：2005-12-07
申请人： Gert Nikolaas Moll , Anneke Kuipers , Rick Rink , Arnold Jacob Mathieu Driessen , Oscar Paul Kuipers
发明人： Gert Nikolaas Moll , Anneke Kuipers , Rick Rink , Arnold Jacob Mathieu Driessen , Oscar Paul Kuipers
IPC分类号： C12P21/06 , C12N15/63 , C12N1/21
CPC分类号： C12N15/625
摘要： The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase. Also provided is a method for producing a polypeptide in a host cell, comprising providing a host cell with a nucleic acid construct according to the invention and allowing expression and secretion of the encoded polypeptide by said host cell, wherein said host cell comprises at least a dehydratase capable of modifying said encoded polypeptide and a non-lantibiotic export system capable of secreting said modified polypeptide.
摘要翻译：本发明涉及在重组宿主细胞中产生异源多肽。更具体地，本发明涉及由一种或多种羊毛硫素合成酶修饰的肽（例如生物活性肽）的产生和分泌。本发明提供了编码多肽的核酸构建体，其包含1）非耐药物出口信号，其被非拉丁比亚出口系统识别; 2）由至少一种羊毛硫抗生素脱水酶（例如LanB）和所述出口信号和所述前导肽的C-末端识别的羊毛硫素前导肽，3）感兴趣的肽含有一个或多个丝氨酸或苏氨酸残基，其中可以通过所述脱水酶进行翻译后脱水。还提供了在宿主细胞中产生多肽的方法，其包括向宿主细胞提供根据本发明的核酸构建体，并允许所述宿主细胞表达和分泌编码的多肽，其中所述宿主细胞至少包含能够修饰所述编码的多肽的脱水酶和能够分泌所述修饰的多肽的非拉链菌素出口系统。

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