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1. 发明授权

US06298859B1 Use of a phenol oxidizing enzyme in the treatment of tobacco 有权
标题翻译：使用酚氧化酶处理烟草
公开(公告)号：US06298859B1
公开(公告)日：2001-10-09
申请号：US09349301
申请日：1999-07-07
申请人： Jesper Vallentin Kierulff , Ole Bill Jørgensen , Tomas Tage Hansen , Anthony James Knox , Yves De Grandpré
发明人： Jesper Vallentin Kierulff , Ole Bill Jørgensen , Tomas Tage Hansen , Anthony James Knox , Yves De Grandpré
IPC分类号： A24B1524
CPC分类号： A24B15/20 , A24B15/24
摘要： A process for preparing tobacco, which process comprises the steps of treating a tobacco material with a phenol oxidising enzyme, such as by extracting tobacco with a solvent to provide an extract and a residue; and treating the extract with a phenol oxidising enzyme such as a laccase. An improved tobacco product having a reduced amount of phenolic compounds. This is an alternative or a supplement to a process in which the phenolic compounds are adsorbed onto the insoluble carrier polyvinylpolypyrrolidone (PVPP). In preferred embodiments, the process includes further steps of removing the oxidised phenolic compound, adding adsorbents such as bentonite; removing and/or inactivating the enzyme; and concentrating the extract. Preferred phenol oxidising enzymes are peroxidases and laccases. The thus treated extract is advantageously re-combined with the tobacco residue and further processed to provide a tobacco article for smoking.
摘要翻译：一种制备烟草的方法，该方法包括用苯酚氧化酶处理烟草材料的步骤，例如通过用溶剂萃取烟草以提供提取物和残余物; 并用苯酚氧化酶如漆酶处理提取物。一种具有减少量的酚类化合物的改进的烟草制品。这是将酚类化合物吸附到不溶性载体聚乙烯吡咯烷酮（PVPP）上的方法的替代或补充。在优选的实施方案中，该方法包括除去氧化的酚类化合物，添加吸附剂如膨润土的步骤; 去除和/或灭活酶; 并浓缩提取物。优选的酚氧化酶是过氧化物酶和漆酶。如此处理的提取物有利地与烟草残余物重新组合并进一步加工以提供用于吸烟的烟草制品。

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