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1. 发明授权

US4039382A Immobilized ribonuclease and alkaline phosphatase 失效
标题翻译：固定核糖核酸酶和碱性磷酸酶
公开(公告)号：US4039382A
公开(公告)日：1977-08-02
申请号：US678459
申请日：1976-04-19
申请人： Minh-Nguy Thang , Annie Guissani born Trachtenberg
发明人： Minh-Nguy Thang , Annie Guissani born Trachtenberg
IPC分类号： C12N11/12 , C07H21/00 , C12N11/14 , C12N11/18 , C12P19/34 , C07G7/02 , C12B1/00
CPC分类号： C07H21/00 , C12N11/18 , C12P19/34 , Y10S435/918
摘要： An insoluble, solid matrix carrying simultaneously several different enzymatic functions, is constituted by the conjoint association by irreversible binding on a previously activated matrix support, of a nuclease selected from the group of ribonucleases A, T.sub.1, T.sub.2, U.sub.2 and an alkaline phosphatease. Free activated groups of the matrix after binding of the enzymes, are neutralized by a free amino organic base. The support is selected from among non-denaturing supports effecting the irreversible physical adsorption of the enzymes, such as supports of glass or quartz beads, highly cross-linked gels of the agarose or cellulose type. Polymers A.sub.n U.sub.OH, A.sub.n C.sub.OH, A.sub.n G.sub.OH and/or oligonucleotides U, C, A or G, of predetermined lengths, can be obtained by fractionating the polyribonucleotides by incubation with the insoluble solid matrix.
摘要翻译：携带同时几种不同酶功能的不溶性固体基质是由预先活化的基质载体，选自核糖核酸酶A，T1，T2，U2和碱性磷酸酶的核酸酶的不可逆结合的共同关联构成的。酶结合后，自由基活化基团被游离氨基有机碱中和。支持物选自影响酶的不可逆物理吸附的非变性支持物，例如玻璃或石英珠的支撑物，琼脂糖或纤维素类型的高度交联的凝胶。具有预定长度的聚合物AnUOH，AnCOH，AnGOH和/或寡核苷酸U，C，A或G可以通过与不溶性固体基质孵育来分级多核糖核苷酸而获得。

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