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1. 发明授权

US06750042B2 Metal binding proteins, recombinant host cells and methods 失效
标题翻译：金属结合蛋白，重组宿主细胞和方法
公开(公告)号：US06750042B2
公开(公告)日：2004-06-15
申请号：US09977137
申请日：2001-10-12
申请人： Anne O. Summers , Jonathan J. Caguiat
发明人： Anne O. Summers , Jonathan J. Caguiat
IPC分类号： C12N500
CPC分类号： C07K14/25
摘要： The present disclosure provides artificial heavy metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metals taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.
摘要翻译：本公开提供了本发明人称为螯合剂的人造重金属结合蛋白。这些螯合剂以较高的亲和力结合镉和/或汞离子。还公开了编码序列，重组DNA分子和包含用于表达所述螯合蛋白的重组DNA分子的重组宿主细胞。在重组宿主细胞或转基因植物中，螯合物可用于结合从污染土壤，地下水或灌溉水中吸收的重金属，并浓缩并螯合这些离子。重组肠道细菌可用于暴露于有毒金属离子（如汞和/或镉）的动物或人类的胃肠道内，其中根据要重新染色的离子重选表达的螯合物。或者，可以将螯合物固定在固体支持物上，以将重金属从包括生物流体的污染的水性介质中结合和浓缩。

2. 发明授权

US5965796A Metal resistance sequences and transgenic plants 失效
标题翻译：金属抗性序列和转基因植物
公开(公告)号：US5965796A
公开(公告)日：1999-10-12
申请号：US878957
申请日：1997-06-19
申请人： Richard Brian Meagher , Anne O. Summers , Clayton L. Rugh
发明人： Richard Brian Meagher , Anne O. Summers , Clayton L. Rugh
IPC分类号： B09C1/10 , C12N9/02 , C12N9/88 , C12N15/29 , C12N15/31 , C12N15/52 , C12N15/82 , A01H4/00 , A01H5/00
CPC分类号： B09C1/105 , C12N15/52 , C12N15/8259 , C12N15/8271 , C12N15/8274 , C12N9/0091 , C12N9/88
摘要： The present invention provides nucleic acid sequences encoding a metal ion resistance protein, which are expressible in plant cells. The metal resistance protein provides for the enzymatic reduction of metal ions including but not limited to divalent Cu, divalent mercury, trivalent gold, divalent cadmium, lead ions and monovalent silver ions. Transgenic plants which express these coding sequences exhibit increased resistance to metal ions in the environment as compared with plants which have not been so genetically modified. Transgenic plants with improved resistance to organometals including alkylmercury compounds, among others, are provided by the further inclusion of plant-expressible organometal lyase coding sequences, as specifically exemplified by the plant-expressible merB coding sequence. Furthermore, these transgenic plants which have been genetically modified to express the metal resistance coding sequences of the present invention can participate in the bioremediation of metal contamination via the enzymatic reduction of metal ions. Transgenic plants resistant to organometals can further mediate remediation of organic metal compounds, for example, alkylmetal compounds including but not limited to methyl mercury, methyl lead compounds, methyl cadmium and methyl arsenic compounds, in the environment by causing the freeing of mercuric or other metal ions and the reduction of the ionic mercury or other metal ions to the less toxic elemental mercury or other metals.
摘要翻译：本发明提供编码金属离子电阻蛋白的核酸序列，其可在植物细胞中表达。金属电阻蛋白提供金属离子的酶还原，包括但不限于二价Cu，二价汞，三价金，二价镉，铅离子和一价银离子。与未经遗传修饰的植物相比，表达这些编码序列的转基因植物表现出对环境中金属离子的增加的抗性。通过进一步包含植物可表达的有机金属裂解酶编码序列，提供对包括烷基汞化合物在内的包括烷基汞化合物在内的具有改善的对有机金属的抗性的转基因植物，如通过植物可表达的merB编码序列特别示例的。此外，经遗传修饰以表达本发明的金属电阻编码序列的这些转基因植物可以通过金属离子的酶还原来参与金属污染物的生物修复。对有机金属有抗性的转基因植物可进一步介导有机金属化合物的修复，例如烷基金属化合物，包括但不限于甲基汞，甲基铅化合物，甲基镉和甲基砷化合物，通过使汞或其它金属的释放离子和将离子汞或其他金属离子还原成毒性较小的元素汞或其他金属。

3. 发明授权

US5668294A Metal resistance sequences and transgenic plants 失效
标题翻译：金属抗性序列和转基因植物
公开(公告)号：US5668294A
公开(公告)日：1997-09-16
申请号：US427097
申请日：1995-04-21
申请人： Richard B. Meagher , Anne O. Summers
发明人： Richard B. Meagher , Anne O. Summers
IPC分类号： B09C1/10 , C12N9/02 , C12N9/88 , C12N15/29 , C12N15/31 , C12N15/52 , C12N15/82 , A01H5/00
CPC分类号： C12N9/0091 , B09C1/105 , C12N15/52 , C12N15/8259 , C12N15/8271 , C12N15/8274 , C12N9/88
摘要： The present invention provides nucleic acid sequences encoding a metal ion resistance protein, which are expressible in plant cells. The metal resistance protein provides for the enzymatic reduction of metal ions including by not limited to divalent Cu, divalent mercury, trivalent gold, divalent cadmium, lead ions and monovalent silver ions. Transgenic plants which express these coding sequences exhibit increased resistance to metal ions in the environment as compared with plants which have not been so genetically modified. Transgenic plants which are resistant to organomercurials including alkylmercury compounds, among others, are provided by the further inclusion of plant-expressible organomercurial lyase coding sequences. Furthermore, these transgenic plants which have been genetically modified to express the metal resistance coding sequences of the present invention can participate in the bioremediation of metal contamination via the enzymatic reduction of metal ions. Transgenic plants resistant to organomercurials can further mediate remediation of alkylmercury compounds in the environment by causing the freeing of mercuric ions and the reduction of the ionic mercury to the less toxic elemental mercury.
摘要翻译：本发明提供编码金属离子电阻蛋白的核酸序列，其可在植物细胞中表达。金属电阻蛋白提供金属离子的酶还原，包括不限于二价Cu，二价汞，三价金，二价镉，铅离子和一价银离子。与未经遗传修饰的植物相比，表达这些编码序列的转基因植物表现出对环境中金属离子的增加的抗性。通过进一步包含植物可表达的有机体裂解酶编码序列，提供对包括烷基汞化合物在内的有机体活性物质具有抗性的转基因植物。此外，经遗传修饰以表达本发明的金属电阻编码序列的这些转基因植物可以通过金属离子的酶还原来参与金属污染物的生物修复。通过使汞离子释放和将离子汞还原成毒性较小的元素汞，可以进一步介导对有机体药物有抗性的转基因植物来调节环境中烷基汞化合物的修复。

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