专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08790893B2 Measuring degree of polymerisation for meningococcal capsular saccharides that contain sialic acid 有权
标题翻译：测量包含唾液酸的脑膜炎球菌荚膜糖的聚合度
公开(公告)号：US08790893B2
公开(公告)日：2014-07-29
申请号：US12875967
申请日：2010-09-03
申请人： Angela Bardotti
发明人： Angela Bardotti
IPC分类号： C12P1/00 , C12N9/00
CPC分类号： A61K39/095 , C08B37/00 , Y10T436/143333
摘要： The degree of polymerisation (DP) is an important parameter for analysis of saccharide antigens, particularly in glycoconjugates. The invention provides methods that can be used to measure DP for capsular saccharides, particularly for meningococcal saccharides e.g. from serogroups W135 and Y. A preferred method is based on reduction of terminal sialic acid residues on saccharides, with DP then being calculated by comparing the molar ratio of total sialic acid to reduced sialic acid.
摘要翻译：聚合度（DP）是分析糖抗原的重要参数，特别是在糖缀合物中。本发明提供了可用于测量荚膜糖的DP的方法，特别是对于脑膜炎球菌糖例如，来自血清群W135和Y.优选的方法是基于糖的末端唾液酸残基的还原，然后通过比较总唾液酸与还原唾液酸的摩尔比计算DP。

2. 发明申请

US20080286300A1 Analysis of Saccharide Vaccines Without Interference 有权
标题翻译：没有干扰的糖类疫苗的分析
公开(公告)号：US20080286300A1
公开(公告)日：2008-11-20
申请号：US10593005
申请日：2005-03-17
申请人： Angela Bardotti , Daniela Proietti , Stefano Ricci
发明人： Angela Bardotti , Daniela Proietti , Stefano Ricci
IPC分类号： A61K39/095 , G01N33/00 , G06F19/00 , A61P31/04
CPC分类号： G01N33/56911 , A61K39/095 , A61K2039/6087 , G01N30/02 , G01N33/5308 , G01N2333/22 , G01N2400/10 , Y10T436/143333 , B01D15/363
摘要： The invention is based on methods that allow analysis of mixed meningococcal saccharides from multiple serogroups even though they share monosaccharide units. With a combination of saccharides from serogroups C, W135 and Y, the invention analyses sialic acid, glucose and galactose content. The glucose and galactose results are used to directly quantify saccharides from serogroups Y and W135, respectively, and the combined glucose and galactose content is subtracted from the sialic acid content to quantify saccharides from serogroup C. The three serogroups can thus be resolved even though their monosaccharide contents overlap. The three different monosaccharide analyses can be performed on the same material, without interference between the monosaccharides and without interference from any other saccharide materials in the composition (e.g. lyophilisation stabilisers). The method can be used to analyse total and free saccharide in conjugate vaccines and simplifies quality control of vaccines containing capsular saccharides from multiple serogroups.
摘要翻译：本发明基于允许分析来自多个血清群的混合脑膜炎球菌糖的方法，即使它们共享单糖单元。利用来自血清群C，W135和Y的糖的组合，本发明分析唾液酸，葡萄糖和半乳糖含量。葡萄糖和半乳糖结果分别用于从血清组Y和W135直接定量糖，并从唾液酸含量中减去葡萄糖和半乳糖的含量，从血清群C中定量糖。因此，三个血清群可以解决，即使它们单糖含量重叠。三种不同的单糖分析可以在相同的材料上进行，而不影响单糖之间的干扰，并且不受组合物中任何其他糖类物质（例如冻干稳定剂）的干扰。该方法可用于分析结合疫苗中的总和游离糖，并简化了来自多个血清群的含有荚膜糖的疫苗的质量控制。

3. 发明授权

US07803583B2 Measuring degree of polymerisation for meningococcal capsular saccharides that contain sialic acid 有权
标题翻译：测量包含唾液酸的脑膜炎球菌荚膜糖的聚合度
公开(公告)号：US07803583B2
公开(公告)日：2010-09-28
申请号：US11597451
申请日：2005-05-23
申请人： Angela Bardotti
发明人： Angela Bardotti
IPC分类号： C12N9/42 , C12P19/04
CPC分类号： A61K39/095 , C08B37/00 , Y10T436/143333
摘要： The degree of polymerisation (DP) is an important parameter for analysis of saccharide antigens, particularly in glycoconjugates. The invention provides methods that can be used to measure DP for capsular saccharides, particularly for meningococcal saccharides e.g. from serogroups W135 and Y. A preferred method is based on reduction of terminal sialic acid residues on saccharides, with DP then being calculated by comparing the molar ratio of total sialic acid to reduced sialic acid.
摘要翻译：聚合度（DP）是分析糖抗原的重要参数，特别是在糖缀合物中。本发明提供了可用于测量荚膜糖的DP的方法，特别是对于脑膜炎球菌糖例如，来自血清群W135和Y.优选的方法是基于糖的末端唾液酸残基的还原，然后通过比较总唾液酸与还原唾液酸的摩尔比计算DP。

4. 发明申请

US20100322958A1 MODIFIED SACCHARIDES 有权
标题翻译：改良的SACCHARIDES
公开(公告)号：US20100322958A1
公开(公告)日：2010-12-23
申请号：US12448709
申请日：2008-01-11
申请人： Angela Bardotti , Alessandro Pianigiani , Francesco Berti , Paolo Costantino
发明人： Angela Bardotti , Alessandro Pianigiani , Francesco Berti , Paolo Costantino
IPC分类号： A61K39/095 , C07H11/00 , C07H13/02 , C07H13/12 , C07H15/04 , C07H11/04 , C07H5/06 , C07K14/00 , C07K14/195 , C07K14/34 , A61K31/70 , A61K31/7024 , A61K38/16 , A61K39/385 , A61K31/715 , A61P31/04 , A61P37/04
CPC分类号： C08B37/00 , A61K39/095 , A61K47/646 , A61K2039/55505 , A61K2039/6037 , C07H13/00 , C08B37/006
摘要： Modified capsular saccharides comprising a blocking group at a hydroxyl group position on at least one of the monosaccharide units of the corresponding native capsular saccharide, wherein the blocking group is of the formula (Ia) or (Ib): —OX—Y (Ia) or —O—R1 (Ib) wherein X is C(O), S(O) or SO2; Y is NR1R2 or R3; R1 is C1-6 alkyl substituted with 1, 2 or 3 groups independently selected from hydroxyl, sulphydryl and amine; R2 is H or C1-6 alkyl; and R3 is C1-6 alkyl; processes for modifying a capsular saccharide with the blocking groups; saccharide-protein conjugates comprising the modified capsular saccharide; processes for making the saccharide-protein conjugates, pharmaceutical compositions comprising the modified capsular saccharides and/or saccharide-protein conjugates; and methods and uses of the same.
摘要翻译：在相应的天然荚膜糖的至少一个单糖单元上的羟基位置上含有封闭基团的修饰的荚膜糖，其中所述封闭基团为式（Ia）或（Ib）：-OX-Y（Ia）或-O-R 1（Ib）其中X为C（O），S（O）或SO 2; Y为NR1R2或R3; R1是独立选自羟基，巯基和胺的1,2或3个基团取代的C 1-6烷基; R2是H或C1-6烷基; 且R 3为C 1-6烷基; 用阻聚基修饰荚膜糖的方法; 包含修饰的荚膜糖的糖 - 蛋白质缀合物; 制备糖 - 蛋白质缀合物的方法，包含改性荚膜糖和/或糖 - 蛋白质缀合物的药物组合物; 以及相同的方法和用途。

5. 发明授权

US07833803B2 Analysis of liquid chromatography eluates 有权
标题翻译：液相色谱洗脱液分析
公开(公告)号：US07833803B2
公开(公告)日：2010-11-16
申请号：US11597254
申请日：2005-05-20
申请人： Daniela Proietti , Angela Bardotti , Stefano Ricci
发明人： Daniela Proietti , Angela Bardotti , Stefano Ricci
IPC分类号： G01N25/08 , G01N30/02
CPC分类号： G01N30/78 , G01N27/44721 , G01N27/4473 , G01N30/02 , G01N30/64 , G01N30/74 , G01N2030/8809 , G01N2030/8836 , B01D15/363
摘要： When analysing saccharides by HPAEC, the eluate from the column is typically analysed using a amperometric detector. According to the invention, amperometric detection is coupled with ultraviolet detection, with both methods being applied to the eluate. Thus the invention provides a method for analysing the eluate from a liquid chromatography column, wherein the eluate is analysed by both (a) amperometric detection and (b) ultraviolet detection. The information content derivable from using both sorts of detection advantageously exceeds that derivable from either of the two detection methods alone.
摘要翻译：当通过HPAEC分析糖时，通常使用电流检测器分析来自色谱柱的洗脱液。根据本发明，电流检测与紫外检测结合，两种方法都应用于洗脱液。因此，本发明提供了一种从液相色谱柱分析洗脱液的方法，其中通过（a）电流检测和（b）紫外检测来分析洗脱液。从使用两种检测导出的信息内容有利地超过了仅从两种检测方法中的任一种得出的信息内容。

6. 发明申请

US20080305126A1 Separation of Unconjugated and Conjugated Saccharide by Solid Phase Extraction 审中-公开
标题翻译：通过固相萃取分离非共轭和共轭糖
公开(公告)号：US20080305126A1
公开(公告)日：2008-12-11
申请号：US10593006
申请日：2005-03-17
申请人： Angela Bardotti , Stefano Ricci , Daniela Proietti
发明人： Angela Bardotti , Stefano Ricci , Daniela Proietti
IPC分类号： A61K39/00 , G01N33/00 , C07H1/06
CPC分类号： G01N33/56911 , A61K39/095 , A61K2039/6087 , G01N30/02 , G01N33/5308 , G01N2333/22 , G01N2400/10 , Y10T436/143333 , B01D15/363
摘要： The invention is based on the use of solid phase extraction for separating conjugated saccharide from unconjugated saccharide in sample, e.g. a vaccine. Solid phase extraction (SPE) provides faster and more reproducible separation of conjugated saccharides from unconjugated saccharides, thereby allowing quantitative separation of these saccharides. The separation of conjugated and unconjugated saccharide using SPE may be advantageously combined with a quantitative conjugate analysis to provide improved quality control for conjugate vaccines. The SPE separation is compatible with existing quantitative conjugate analysis techniques, such as high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD).
摘要翻译：本发明是基于使用固相萃取来从样品中的共轭糖与未结合的糖分离的。一种疫苗固相萃取（SPE）提供了更快，更可重复地将共轭糖与未结合的糖分离，从而允许这些糖的定量分离。使用SPE分离共轭和未偶联的糖可以有利地与定量缀合物分析组合以提供缀合疫苗的改进的质量控制。 SPE分离与现有的定量共轭分析技术相兼容，例如具有脉冲电流检测（HPAEC-PAD）的高效阴离子交换色谱法。

7. 发明申请

US20120171241A1 ANALYSIS OF SACCHARIDE VACCINES WITHOUT INTERFERENCE 审中-公开
标题翻译：没有干扰的沙门菌疫苗的分析
公开(公告)号：US20120171241A1
公开(公告)日：2012-07-05
申请号：US13418211
申请日：2012-03-12
申请人： Angela Bardotti , Daniela Proietti , Stefano Ricci
发明人： Angela Bardotti , Daniela Proietti , Stefano Ricci
IPC分类号： A61K39/385 , A61P37/04 , G01N33/48
CPC分类号： G01N33/56911 , A61K39/095 , A61K2039/6087 , G01N30/02 , G01N33/5308 , G01N2333/22 , G01N2400/10 , Y10T436/143333 , B01D15/363
摘要： The invention is based on methods that allow analysis of mixed meningococcal saccharides from multiple serogroups even though they share monosaccharide units. With a combination of saccharides from serogroups C, W135 and Y, the invention analyses sialic acid, glucose and galactose content. The glucose and galactose results are used to directly quantify saccharides from serogroups Y and W135, respectively, and the combined glucose and galactose content is subtracted from the sialic acid content to quantify saccharides from serogroup C. The three serogroups can thus be resolved even though their monosaccharide contents overlap. The three different monosaccharide analyses can be performed on the same material, without interference between the monosaccharides and without interference from any other saccharide materials in the composition (e.g. lyophilisation stabilisers). The method can be used to analyse total and free saccharide in conjugate vaccines and simplifies quality control of vaccines containing capsular saccharides from multiple serogroups.
摘要翻译：本发明基于允许分析来自多个血清群的混合脑膜炎球菌糖的方法，即使它们共享单糖单元。利用来自血清群C，W135和Y的糖的组合，本发明分析唾液酸，葡萄糖和半乳糖含量。葡萄糖和半乳糖结果分别用于从血清组Y和W135直接定量糖，并从唾液酸含量中减去葡萄糖和半乳糖的含量，从血清群C中定量糖。因此，三个血清群可以被解决，即使它们单糖含量重叠。三种不同的单糖分析可以在相同的材料上进行，而不影响单糖之间的干扰，并且不受组合物中任何其他糖类物质（例如冻干稳定剂）的干扰。该方法可用于分析结合疫苗中的总和游离糖，并简化了来自多个血清群的含有荚膜糖的疫苗的质量控制。

8. 发明授权

US08137680B2 Analysis of saccharide vaccines without interference 有权
标题翻译：分析糖类疫苗无干扰
公开(公告)号：US08137680B2
公开(公告)日：2012-03-20
申请号：US10593005
申请日：2005-03-17
申请人： Angela Bardotti , Daniela Proietti , Stefano Ricci
发明人： Angela Bardotti , Daniela Proietti , Stefano Ricci
IPC分类号： A61K39/095 , G01N31/00 , G01N31/02 , G01N21/00 , G01N30/02 , G01J3/42
CPC分类号： G01N33/56911 , A61K39/095 , A61K2039/6087 , G01N30/02 , G01N33/5308 , G01N2333/22 , G01N2400/10 , Y10T436/143333 , B01D15/363
摘要： The invention is based on methods that allow analysis of mixed meningococcal saccharides from multiple serogroups even though they share monosaccharide units. With a combination of saccharides from serogroups C, W135 and Y, the invention analyses sialic acid, glucose and galactose content. The glucose and galactose results are used to directly quantify saccharides from serogroups Y and W135, respectively, and the combined glucose and galactose content is subtracted from the sialic acid content to quantify saccharides from serogroup C. The three serogroups can thus be resolved even though their monosaccharide contents overlap. The three different monosaccharide analyses can be performed on the same material, without interference between the monosaccharides and without interference from any other saccharide materials in the composition (e.g. lyophilisation stabilisers). The method can be used to analyse total and free saccharide in conjugate vaccines and simplifies quality control of vaccines containing capsular saccharides from multiple serogroups.
摘要翻译：本发明基于允许分析来自多个血清群的混合脑膜炎球菌糖的方法，即使它们共享单糖单元。利用来自血清群C，W135和Y的糖的组合，本发明分析唾液酸，葡萄糖和半乳糖含量。葡萄糖和半乳糖结果分别用于从血清组Y和W135直接定量糖，并从唾液酸含量中减去葡萄糖和半乳糖的含量，从血清群C中定量糖。因此，三个血清群可以被解决，即使它们单糖含量重叠。三种不同的单糖分析可以在相同的材料上进行，而不影响单糖之间的干扰，并且不受组合物中任何其他糖类物质（例如冻干稳定剂）的干扰。该方法可用于分析结合疫苗中的总和游离糖，并简化了来自多个血清群的含有荚膜糖的疫苗的质量控制。

9. 发明申请

US20110159029A1 MEASURING DEGREE OF POLYMERISATION FOR MENINGOCOCCAL CAPSULAR SACCHARIDES THAT CONTAIN SIALIC ACID 有权
标题翻译：含有酸的肉桂酸脂肪酸聚合物的聚合度测定
公开(公告)号：US20110159029A1
公开(公告)日：2011-06-30
申请号：US12875967
申请日：2010-09-03
申请人： Angela BARDOTTI
发明人： Angela BARDOTTI
IPC分类号： A61K39/385 , A61P37/04 , A61P31/04
CPC分类号： A61K39/095 , C08B37/00 , Y10T436/143333
摘要： The degree of polymerisation (DP) is an important parameter for analysis of saccharide antigens, particularly in glycoconjugates. The invention provides methods that can be used to measure DP for capsular saccharides, particularly for meningococcal saccharides e.g. from serogroups W135 and Y. A preferred method is based on reduction of terminal sialic acid residues on saccharides, with DP then being calculated by comparing the molar ratio of total sialic acid to reduced sialic acid.
摘要翻译：聚合度（DP）是分析糖抗原的重要参数，特别是在糖缀合物中。本发明提供了可用于测量荚膜糖的DP的方法，特别是对于脑膜炎球菌糖例如，来自血清群W135和Y.优选的方法是基于糖的末端唾液酸残基的还原，然后通过比较总唾液酸与还原唾液酸的摩尔比计算DP。

10. 发明申请

US20100219335A1 Liquid Chromatography-Mass Spectrometry Analysis of Samples Using Ionic Eluent Comprising a Volatile Ionic Salt 审中-公开
标题翻译：使用包含挥发性离子盐的离子洗脱液的样品的液相色谱 - 质谱分析
公开(公告)号：US20100219335A1
公开(公告)日：2010-09-02
申请号：US12085507
申请日：2006-12-05
申请人： Angela Bardotti , Alessandro Pianigiani
发明人： Angela Bardotti , Alessandro Pianigiani
IPC分类号： H01J49/04 , H01J49/26 , A61K39/02
CPC分类号： G01N30/7233 , C07K1/18 , G01N30/02 , G01N30/96 , B01D15/363 , B01D15/426
摘要： The use of an ionic eluent in ion exchange chromatography for analysing a sample comprising an analyte, wherein the ionic eluent comprises a volatile ionic salt, in particular an ammonium salt, is described. Such eluents are shown to be compatible with mass spectrometry (MS), providing clean mass spectra of the analyte. Furthermore, eluates from ion exchange chromatography may be advantageously analysed with MS without additional on-line or off-line devices for desalting or suppressing salts in the eluent. Important information concerning the chemical structure and composition of a sample may therefore be obtained with ion chromatography-MS by utilising the invention. The invention also provides a method of analysing a sample (e.g. a vaccine) comprising an analyte (e.g. a saccharide) by ion chromotography-MS by employing an ionic eluent, wherein the ionic eluent comprises a volatile ionic salt. An apparatus for analysing a sample comprising an analyte is also disclosed.
摘要翻译：在离子交换层析中使用离子洗脱液用于分析包含分析物的样品，其中离子洗脱液包含挥发性离子盐，特别是铵盐。显示这种洗脱液与质谱（MS）相容，提供分析物的清晰质谱。此外，离子交换层析的洗脱液可以有利地用MS分析，而无需额外的在线或离线装置用于在洗脱液中脱盐或抑制盐。因此，通过利用本发明，可以通过离子色谱法MS获得关于样品的化学结构和组成的重要信息。本发明还提供了通过使用离子洗脱液通过离子色谱-MS分析包含分析物（例如糖）的样品（例如疫苗）的方法，其中离子洗脱液包含挥发性离子盐。还公开了一种用于分析包含分析物的样品的装置。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式