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1. 发明授权

US5792932A Plant promoter, microorganisms and plant cells containing a unit for the expression of a protein of interest comprising said promoter 失效
标题翻译：植物启动子，微生物和含有用于表达目的蛋白质的单元的植物细胞包含所述启动子
公开(公告)号：US5792932A
公开(公告)日：1998-08-11
申请号：US525507
申请日：1995-09-22
申请人： Yves Marco , Dominique Roby , Michel Schneider , Alain Toppan
发明人： Yves Marco , Dominique Roby , Michel Schneider , Alain Toppan
IPC分类号： C07K14/415 , C12N1/21 , C12N15/29 , C12N15/82 , C07H21/04 , C12N1/00 , C12N5/14
CPC分类号： C12N15/8239 , C07K14/415 , C12N15/8222 , C12N15/8227 , C12N15/8229 , C12N15/8238
摘要： The present invention relates to a plant promoter which comprises the sequence (B) �SEQ ID NO: 4! below: ##STR1## or a sequence having a high degree of homology with the sequence (B). Application: Protection of plants by genetic engineering and especially defense of plants in stress conditions
摘要翻译： PCT No.PCT / FR94 / 00316 Sec。 371日期1995年9月22日 102（e）1995年9月22日PCT 1994年3月23日PCT公布。 WO94 / 21793 PCT出版物日期9月29日，1994The本发明涉及一种植物启动子，其包含序列（B）[SEQ ID NO：4]下面：TCAAATGAAATACACATAAGAAGCACATAA + TR ATTTAAATGCCGTATTAAACTTACAGTATA60 CTATAGCGGAAGTTGGCTTGATAAAGGAAC + TR GCTGAGGAGAGTAGCCGATGGTGAAACACT120 AACATCAAGTGCAAAAGAAAGAAAAACTGA + TR AAACAGAAGATGAATGTTTGAAGTGGGTAA180 AAGATTACTTAAAAGATAGGTTTGGTTAAC + TR

2. 发明授权

US09994863B2 Glyphosate tolerant corn event VCO-O1981-5 and kit and method for detecting the same 有权
公开(公告)号：US09994863B2
公开(公告)日：2018-06-12
申请号：US14235219
申请日：2012-07-26
申请人： Lori Artim Artim Mann , Vadim Beilinson , Nadine Carozzi , Rebekah Deter , Brian Vande Berg , Alain Toppan , Laurent Beuf , Georges Freyssinet
发明人： Lori Artim Artim Mann , Vadim Beilinson , Nadine Carozzi , Rebekah Deter , Brian Vande Berg , Alain Toppan , Laurent Beuf , Georges Freyssinet
IPC分类号： A01H5/10 , C12N15/82 , C12N9/10 , C12Q1/68
CPC分类号： C12N15/8275 , A01H5/10 , C12N9/1092 , C12Q1/6895 , C12Y205/01019 , Y10T436/143333
摘要： The present invention relates to the field of plant transformation with genes conferring tolerance to glyphosate. The invention particularly relates to a maize (corn) plant transformed with a gene encoding an EPSPS providing the plant tolerance to an application of glyphosate under conditions where this herbicide is effective in killing weeds.The invention particularly concerns an elite transformation event VCO-Ø1981-5 comprising the gene construct and means, kits and methods for detecting the presence of the said elite event.

3. 发明申请

US20140325697A1 Glyphosate tolerant corn event VCO-O1981-5 and kit and method for detecting the same 有权
标题翻译：草甘膦耐受性玉米事件VCO-O1981-5及其检测方法
公开(公告)号：US20140325697A1
公开(公告)日：2014-10-30
申请号：US14235219
申请日：2012-07-26
申请人： Lori Artim Artim Mann , Vadim Beilinson , Nadine Carozzi , Rebekah Deter , Brian Vande Berg , Alain Toppan , Laurent Beuf , Georges Freyssinet
发明人： Lori Artim Artim Mann , Vadim Beilinson , Nadine Carozzi , Rebekah Deter , Brian Vande Berg , Alain Toppan , Laurent Beuf , Georges Freyssinet
IPC分类号： C12N15/82 , C12Q1/68
CPC分类号： C12N15/8275 , A01H5/10 , C12N9/1092 , C12Q1/6895 , C12Y205/01019 , Y10T436/143333
摘要： The present invention relates to the field of plant transformation with genes conferring tolerance to glyphosate. The invention particularly relates to a maize (corn) plant transformed with a gene encoding an EPSPS providing the plant tolerance to an application of glyphosate under conditions where this herbicide is effective in killing weeds.The invention particularly concerns an elite transformation event VCO-Ø1981-5 comprising the gene construct and means, kits and methods for detecting the presence of the said elite event.
摘要翻译：本发明涉及具有赋予对草甘膦耐受性的基因的植物转化领域。本发明特别涉及用编码EPSPS的基因转化的玉米（玉米）植物，其在该除草剂有效杀死杂草的条件下提供植物对施用草甘膦的耐受性。本发明特别涉及包含基因构建的精英变换事件VCO-Ø1981-5以及用于检测所述精英事件的存在的手段，工具箱和方法。

4. 发明授权

US07094952B1 Method for obtaining transgenic plants expressing a protein with activity producing hydrogen peroxide by transformation by agrobacterium rhizogenes 有权
标题翻译：通过农杆菌转化获得表达具有产生过氧化氢活性的蛋白质的转基因植物的方法
公开(公告)号：US07094952B1
公开(公告)日：2006-08-22
申请号：US09821463
申请日：1999-10-08
申请人： Michel Pagniéz , René Grison , Alain Toppan
发明人： Michel Pagniéz , René Grison , Alain Toppan
IPC分类号： C12N15/82 , C12N15/87 , C12N5/10 , C07H26/04
CPC分类号： C12N9/0008 , C12N9/16 , C12N9/18 , C12N15/8205 , C12N15/8209
摘要： The subject of the present invention is a method for obtaining transgenic plants expressing a protein with H2O2 producing activity, which comprises the following steps of: (a) transforming plant cells with Agrobacterium rhizogenes containing a vector carrying a gene encoding a protein producing H2O2 in a context which allows its expression in the plant; (b) selecting the transformants which contain and express this gene, by a peroxidase-based colorimetric test; (c) regenerating the plants from the roots selected and monitoring the expression of the plantlets obtained by a peroxidase-based colorimetric test; (d) sorting according to phenotype and optionally carrying out the molecular analysis of the progeny of the transgenic plants, allowing the selection or the confirmation of transgenic plants obtained containing only the transgene and not the T-DNA specific to A. rhizogenes. Application: Obtaining of transgenic plants
摘要翻译：本发明的主题是获得表达具有H 2 O 2 O 2生产活性的蛋白质的转基因植物的方法，其包括以下步骤：（a）转化植物在含有能够在植物中表达的情况下含有携带编码产生H 2 O 2 O 2的蛋白质的基因的载体的发根农杆菌的细胞; （b）通过基于过氧化物酶的比色试验选择含有并表达该基因的转化体; （c）从选择的根中再生植物并监测通过基于过氧化物酶的比色试验获得的小植物的表达; （d）根据表型进行分选并任选地对转基因植物的后代进行分子分析，从而允许选择或确认仅含有转基因而不是对发根发酵菌特异性的T-DNA的转基因植物。应用：获得转基因植物

5. 发明授权

US5859340A Recombinant gene coding for a protein having endochitinase activity 失效
标题翻译：编码具有内切壳酶活性的蛋白质的重组基因
公开(公告)号：US5859340A
公开(公告)日：1999-01-12
申请号：US475427
申请日：1995-06-07
申请人： Michel Dubois , Rene Grison , Jean-Jacques Leguay , Annie Pignard , Alain Toppan
发明人： Michel Dubois , Rene Grison , Jean-Jacques Leguay , Annie Pignard , Alain Toppan
IPC分类号： A01H5/00 , C12N1/21 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/29 , C12N15/56 , C12N15/82 , C12N15/62
CPC分类号： C12Y302/01017 , C12N15/8279 , C12N9/2442 , C12Y302/01014
摘要： Recombinant gene coding for a protein having endochitinase activity or for a precursor thereof which comprises the sequence of SEQ ID NO:1 below:Gly Gly Asp Leu Gly Ser VaL Ile Ser Asn Ser Met Phe Asp Gln Met Leu Lys His Arg Asn Glu Asn Ser Cys Gln Gly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile Thr Ala Ala Arg Ser Phe Pro Gly, Phe Gly Thr Ser Gly Asp Ile Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe Ala Gln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser Ala Pro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu Arg Gly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser Ser Gln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly Arg Gly Pro Ile Gln Ile Ser His Asn Tyr Asn Tyr Gly Pro Cys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn Pro Asp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys Thr Ala Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys Pro Ser Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro Ser Ala Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe Gly Val Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys Gly Arg Gly Asn Asp Asn Arg Val Gln Asp Arg Ile Gly Phe Tyr Arg Arg Tyr Cys Gly Ile Leu Gly Val Ser Pro Gly Asp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly Asn Gly Leu Leu Val Asp Thr Met.Application: For obtaining plants resisting to pathogenic agents.
摘要翻译：编码具有内切素酶活性的蛋白质或其前体的重组基因，其包含以下SEQ ID NO：1的序列：Gly Gly Asp Leu Gly Ser VaL Ile Ser Asn Ser Met Phe Asp Gln Met Leu Lys His Arg Asn Glu Asn Ser Cys Gln Gly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile Thr Ala Ala Arg Ser Phe Pro Gly，Phe Gly Thr Ser Gly Asp Ile Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe Ala Gln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser Ala Pro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu Arg Gly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser Ser Gln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly Arg Gly Pro Ile Gln Ile Ser 他的Asn Tyr Asn Tyr Gly Pro Cys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn Pro Asp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys Thr Ala Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys Pro Ser Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro Ser Ala Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe Gly Val Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys Gly Arg Gly Asn Asp Asn A rg Val Gln Asp Arg Ile Gly Phe Tyr Arg Arg Tyr Cys Gly Ile Leu Gly Val Ser Pro Gly Asp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly Asn Gly Leu Leu Val Asp Thr Met。用途：用于获得抗病原体的植物。

6. 发明授权

US5477001A Recombinant DNA coding for a novel protein having .beta.-1,3-glucanase activity, bacteria containing this DNA, transformed plant cells and plants 失效
标题翻译：编码具有β-1,3-葡聚糖酶活性的新蛋白质的重组DNA，含有该DNA的细菌，转化的植物细胞和植物
公开(公告)号：US5477001A
公开(公告)日：1995-12-19
申请号：US966187
申请日：1993-01-25
申请人： Catherine Sass , Jean-Jacques Leguay , Rene Grison , Alain Toppan
发明人： Catherine Sass , Jean-Jacques Leguay , Rene Grison , Alain Toppan
IPC分类号： A01H5/00 , C12N1/21 , C12N5/10 , C12N9/24 , C12N15/00 , C12N15/56 , C12N15/82 , C12R1/19 , C12N15/29
CPC分类号： C12Y302/01039 , C12N15/8279 , C12N9/2402 , C12Y302/01058
摘要： The invention provides a protein, isolated from soybean, having .beta.-1,3-glucanase activity and comprising the amino acid sequence of SEQ ID No: 1. DNA constructs encoding said protein, and transformed plants expressing the protein, are also provided.
摘要翻译： PCT No.PCT / FR92 / 00268 Sec。 371日期：1993年1月25日 102（e）日期1993年1月25日PCT提交1992年3月25日PCT公布。出版物WO92 / 16632 本发明提供了一种从大豆中分离得到的具有β-1,3-葡聚糖酶活性并包含SEQ ID No：1的氨基酸序列的蛋白质。编码所述蛋白质的DNA构建体和表达该蛋白质的转化植物蛋白质。

7. 发明授权

US5932698A Recombinant gene coding for a protein having endochitinase activity 失效
标题翻译：编码具有内切壳酶活性的蛋白质的重组基因
公开(公告)号：US5932698A
公开(公告)日：1999-08-03
申请号：US842165
申请日：1992-05-01
申请人： Michel Dubois , Rene Grison , Jean-Jacques Leguay , Annie Pignard , Alain Toppan
发明人： Michel Dubois , Rene Grison , Jean-Jacques Leguay , Annie Pignard , Alain Toppan
IPC分类号： A01H5/00 , C12N1/21 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/29 , C12N15/56 , C12N15/82 , C12N15/62
CPC分类号： C12Y302/01017 , C12N15/8279 , C12N9/2442 , C12Y302/01014
摘要： Recombinant gene coding for a protein having endochitinase activity or for a precursor thereof which comprises the sequence (SEQ ID NO:1) below:Gly Gly Asp Leu Gly Ser Val Ile Ser Asn Ser Met PheAsp Gln Met Leu Lys His Arg Asn Glu Asn Ser Cys GlnGly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile ThrAla Ala Arg Ser Phe Pro Gly Phe Gly Thr Ser Gly AspIle Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe AlaGln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser AlaPro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu ArgGly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser SerGln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly ArgGly Pro Ile Gln Ile Ser His Asn Tyr Asn Tyr Gly ProCys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn ProAsp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys ThrAla Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys ProSer Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro SerAla Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe GlyVal Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys GlyArg Gly Asn Asp Asn Arg Val Gln Asp Arg Ile Gly PheTyr Arg Arg Try Cys Gly Ile Leu Gly Val Ser Pro GlyAsp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly AsnGly Leu Leu Val Asp Thr Met.Application: For obtaining plants resisting to pathogenic agents.
摘要翻译： PCT No.PCT / FR91 / 00607 Sec。 371日期：1992年5月1日 102（e）日期1992年5月1日PCT PCT 1991年7月24日PCT公布。出版物WO92 / 01792 日期1992年2月6日编码含有内切素酶活性的蛋白质的重组基因或其前体包含以下序列（SEQ ID NO：1）： - Gly Gly Asp Leu Gly Ser Val Ile Ser Asn Ser Met Phe - Asp Asp Met Leu Lys His Arg Asn Glu Asn Ser Cys Gln - - Gly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile Thr - - Ala Ala Arg Ser Phe Pro Gly Phe Gly Thr Ser Gly Asp - - Ile Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe Ala - - Gln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser Ala - - Pro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu Arg - - Gly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser Ser - - Gln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly Arg - - Gly Pro Ile Gln Ile Ser His Asn Tyr Asn Tyr Gly Pro - - Cys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn Pro - - Asp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys Thr - - Ala Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys Pro - - Ser Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro Ser - - Ala Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe Gly - - Val Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys Gly - - Arg Gly Asn Asp Asn Arg Val Gln Asp Arg Ile Gly Phe - - Tyr Arg Arg尝试Cys Gly Ile Leu Gly Val Ser Pro Gly - - Asp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly Asn - - Gly Leu Leu Val Asp Thr Met。 - 应用：用于获得抗病原体的植物。

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