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1. 发明授权

US07851157B1 Oligonucleotide derivative, probe for detection of gene, and DNA chip 有权
标题翻译：寡核苷酸衍生物，用于检测基因的探针和DNA芯片
公开(公告)号：US07851157B1
公开(公告)日：2010-12-14
申请号：US11892673
申请日：2007-08-24
申请人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo , Kazushi Sakamoto , Takeshi Sasami
发明人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo , Kazushi Sakamoto , Takeshi Sasami
IPC分类号： C12Q1/68 , C07G3/00 , C07H21/02 , C07H21/00 , C07H21/04
CPC分类号： C07H21/04 , A61K48/00 , C07H19/04 , C07H19/14 , C07H19/173 , C12N15/111 , C12N15/113 , C12N2310/32 , C12N2310/33 , C12N2320/11 , C12Q1/6837 , C12Q2525/117
摘要： To provide an oligonucleotide derivative that can be used without a problem of falling of a DNA probe from a support.The oligonucleotide derivative of the present invention is represented by the following General Formula (1). The oligonucleotide derivative of the present invention can be used in a DNA chip, a microarray, and so on and further used in a method of detecting gene and a method of regulating gene expression, for example.
摘要翻译：提供可以使用DNA寡核苷酸衍生物而没有脱离载体的DNA探针的问题。本发明的寡核苷酸衍生物由以下通式（1）表示。本发明的寡核苷酸衍生物可用于DNA芯片，微阵列等，并进一步用于检测基因的方法和调节基因表达的方法。

2. 发明申请

US20080051571A1 Silyl Linker For Solid-Phase Synthesis Of Nucleic Acid 有权
标题翻译：用于固相合成核酸的硅烷基连接剂
公开(公告)号：US20080051571A1
公开(公告)日：2008-02-28
申请号：US10590267
申请日：2005-02-10
申请人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
发明人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
IPC分类号： C07H1/00 , C07F7/02 , C07H21/00
CPC分类号： C07H19/173 , C07F7/0896 , C07H19/073 , C07H21/04
摘要： The purpose of the invention is to develop a silyl linker that can be efficiently introduced on a solid-phase support used for the synthesis of nucleic acid oligomers such as DNA. The present invention relates to a silyl linker for use in the solid-phase synthesis of nucleic acid, comprised of a compound of the general formula or its ester or salt: H—(R1)Si(R2)—(C6H4)—CONH-(A)-COOH (I) wherein each of R1 and R2 is an alkyl or aryl group, and (A) represents a spacer moiety; a 3′-end nucleoside unit having said compound linked via an oxygen atom to the 3-position of a sugar of the nucleoside or its derivative, a solid-phase support having the 3′-end nucleoside unit, and a method for synthesis of nucleic acid oligomer with the use of said solid-phase support.
摘要翻译：本发明的目的是开发可以有效地引入用于合成核酸寡聚物如DNA的固相载体上的甲硅烷基接头。本发明涉及用于固相合成核酸的甲硅烷基连接体，其由通式或其酯或盐的化合物组成：<？in-line-formula description =“In-line Formulas”end =“铅”→H（R 1）Si（R 2） - （C 6 H 4） - CONH-（A）-COOH（I） -line-formula description =“In-line Formulas”end =“tail”？>其中R1和R2各自为烷基或芳基，（A）表示间隔基; 具有通过氧原子与核苷或其衍生物的糖的3-位连接的所述化合物的3'-末端核苷单元，具有3'-末端核苷单元的固相载体和合成使用所述固相载体的核酸寡聚物。

3. 发明申请

US20080039620A1 3'-End Nucleoside Unit Comprising Phosphoramidite 有权
标题翻译：包含亚磷酰胺的3'-末端核苷单位
公开(公告)号：US20080039620A1
公开(公告)日：2008-02-14
申请号：US10590268
申请日：2005-02-10
申请人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
发明人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
IPC分类号： C07H21/04 , C08F8/40
CPC分类号： C07F9/65616 , C07F9/65586 , C07H21/04 , Y02P20/55
摘要： The purpose of the present invention is therefore to provide a method for binding a 3′-end nucleoside unit comprising any base to a hydroxyl group on a solid-phase support under completely the same condition as in DNA chain elongation reaction. The present invention relates to a 3′-end nucleoside unit comprising phosphoramidite that is a compound represented by the following formula: (N)—O—(R1)Si(R2)-(C6H4)—(CH2)n-O—P(OR3)N(R4)(R5) (I) wherein (N) represents any nucleoside or its derivative, each of R1, R2, R4 and R5 is an alkyl or aryl group, R3 is a phosphate-protecting group, and n is an integer of from 1 to 5; a solid-phase support having said 3′-end nucleoside unit; and a method for the synthesis of a nucleic acid oligomer with the use of said solid-phase support.
摘要翻译：因此，本发明的目的是提供一种在与DNA链伸长反应完全相同的条件下将包含任何碱基的3'-末端核苷单元与固相载体上的羟基结合的方法。本发明涉及包含亚磷酰胺的3'-末端核苷单位，该亚磷酰胺是下式表示的化合物：＆lt;＆lt;线内式记载=“在线式”末端=“铅”→（N） - O-（R 1）Si（R 2） - （C 6 H 4） - （CH 2）nOP（OR 3）N（R 4）其中（N）表示任何核苷或其衍生物，R 1，R 2，R 4和R 5各自为烷基或芳基，R3是磷酸酯保护基，n是1至5的整数; 具有所述3'-末端核苷单元的固相载体; 以及使用所述固相载体合成核酸寡聚物的方法。

4. 发明授权

US07943758B2 3′-end nucleoside unit comprising phosphoramidite 有权
标题翻译：包含亚磷酰胺的3'-末端核苷单位
公开(公告)号：US07943758B2
公开(公告)日：2011-05-17
申请号：US10590268
申请日：2005-02-10
申请人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
发明人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
IPC分类号： C07H19/10 , C07H19/20
CPC分类号： C07F9/65616 , C07F9/65586 , C07H21/04 , Y02P20/55
摘要： Methods of synthesizing nucleic acid oligomers on a solid-phase support having a 3′-end nucleoside unit introduced thereon-as represented by formula II: wherein of formula II represents a 2′-deoxyribonucleoside or its N-protected derivative, the substituent —O—(R1)Si(R2)—(C6H3R6)—(CH2)n—O—P(OR3)XO)—(CH2)n is attached at the 3′ position of the sugar moiety of the nucleoside substituent; each of R1 and R2 is an alkyl or optionally substituted aryl group, wherein the optionally substituted aryl group has a substituent selected from the group consisting of C1-4 alkyl, nitro, cyano, halo and methoxyl; R3 is a protecting group; X is S or O; R7 is H or 4,4′-dimethoxytrityl; each n is an integer of from 1 to 5; and the solid-phase support has hydroxyl groups on its surface.
摘要翻译：在其上引入3'-末端核苷单元的固相载体上合成核酸低聚物的方法 - 如式II所示：其中式II表示2'-脱氧核糖核苷或其N-保护的衍生物，取代基-O - （R1）Si（R2） - （C6H3R6） - （CH2）n-O-P（OR3）XO） - （CH2）n连接在核苷取代基的糖部分的3'位置; R 1和R 2各自为烷基或任选取代的芳基，其中任选取代的芳基具有选自C 1-4烷基，硝基，氰基，卤素和甲氧基的取代基。 R3是保护基; X是S或O; R7是H或4,4'-二甲氧基三苯甲基; 每个n是1至5的整数; 并且固相载体在其表面上具有羟基。

5. 发明授权

US07807821B1 Method for the synthesis of nucleic acid without protecting base moiety 有权
标题翻译：不保护碱基部分合成核酸的方法
公开(公告)号：US07807821B1
公开(公告)日：2010-10-05
申请号：US10591172
申请日：2005-02-24
申请人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
发明人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
IPC分类号： C07H21/00
CPC分类号： C07H21/04
摘要： A phosphoramidite method for the synthesis of a nucleic acid oligomer without protecting the base moiety characterized in that a 3′ or 5′ hydroxyl group of a nucleotide is reacted with a nucleoside phosphoramidite, a cyclonucleoside phosphoramidite, a 2′-substituted nucleoside phosphoramidite, a 4′-substituted nucleoside phosphoramidite, or a 2′,4′-di-substituted nucleoside phosphoramidite to produce a phosphodiester linkage. The phosphoramidite is contacted with an activator containing both a) hydroxybenzotriazole-1-ol (HOBt), a mono-substituted HOBt, a di-substituted HOBt, or a di-substituted phenol and b) imidazole, tetrazole, benzimidazoletriflate (BIT), 4-ethylthiotetrazole, imidazolium triflate(trifluoromethane sulfonate) or 4,5-dicyanoimidazole.
摘要翻译：用于合成核酸寡聚物而不保护碱基部分的亚磷酰胺方法，其特征在于核苷酸的3'或5'羟基与核苷亚磷酰胺，环核苷亚磷酰胺，2'-取代的核苷亚磷酰胺， 4'-取代的核苷亚磷酰胺或2'，4'-二取代的核苷亚磷酰胺，以产生磷酸二酯键。将亚磷酰胺与包含a）羟基苯并三唑-1-醇（HOBt），单取代HOBt，二取代HOBt或二取代苯酚和b）咪唑，四唑，苯并咪唑三氟甲磺酸酯（BIT），四氟乙磺酸四氢呋喃，三氟甲烷磺酸咪唑鎓或4,5-二氰基咪唑。

6. 发明授权

US07786296B2 Silyl linker for solid-phase synthesis of nucleic acid 有权
标题翻译：用于固相合成核酸的硅烷基接头
公开(公告)号：US07786296B2
公开(公告)日：2010-08-31
申请号：US10590267
申请日：2005-02-10
申请人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
发明人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
IPC分类号： C07H19/00 , C07H19/048
CPC分类号： C07H19/173 , C07F7/0896 , C07H19/073 , C07H21/04
摘要： The purpose of the invention is to develop a silyl linker that can be efficiently introduced on a solid-phase support used for the synthesis of nucleic acid oligomers such as DNA. The present invention relates to a silyl linker for use in the solid-phase synthesis of nucleic acid, comprised of a compound of the general formula or its ester or salt: H—(R1)Si(R2)-(C6H4)—CONH-(A)-COOH (I) wherein each of R1 and R2 is an alkyl or aryl group, and (A) represents a spacer moiety; a 3′-end nucleoside unit having said compound linked via an oxygen atom to the 3-position of a sugar of the nucleoside or its derivative, a solid-phase support having the 3′-end nucleoside unit, and a method for synthesis of nucleic acid oligomer with the use of said solid-phase support.
摘要翻译：本发明的目的是开发可以有效地引入用于合成核酸寡聚物如DNA的固相载体上的甲硅烷基接头。本发明涉及用于固相合成核酸的甲硅烷基连接体，其由通式或其酯或盐的化合物组成：H-（R1）Si（R2） - （C6H4）-CONH- （A）-COOH（I）其中R1和R2各自为烷基或芳基，（A）表示间隔基; 具有通过氧原子与核苷或其衍生物的糖的3-位连接的所述化合物的3'-末端核苷单元，具有3'-末端核苷单元的固相载体和合成使用所述固相载体的核酸寡聚物。

7. 外观设计

USD801479S1 Electromagnetic valve 有权
公开(公告)号：USD801479S1
公开(公告)日：2017-10-31
申请号：US29574669
申请日：2016-08-18
申请人： Yoshitada Doi , Akihiro Ohkubo , Takahiro Kimura
设计人： Yoshitada Doi , Akihiro Ohkubo , Takahiro Kimura

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