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    • 2. 发明授权
    • Anthocyanin producing callus line in cultures of panax sikkimensis and a method of producing panax sikkimensis line capable of producing anthocyanin
    • 花生素生产猕猴桃培养物中的花青素生产线和一种能够产生花青素的西葫芦系的生产方法
    • US06368860B1
    • 2002-04-09
    • US09538425
    • 2000-03-29
    • Archana MathurAnita GangwarAjay Kumar MathurRajender Singh SangwanDharam Chand Jain
    • Archana MathurAnita GangwarAjay Kumar MathurRajender Singh SangwanDharam Chand Jain
    • C12N500
    • A01H4/005C12N5/04C12P23/00
    • The present invention relates to an anthocyanin producing callus line in cultures of Panax sikkimensis (an Indian species of ginseng) said line comprising: (a) Characteristic pink-purple pigmentation, (b) Growth index of about 221-450 in about 50-80 days of culture period, (c) Yield of anthocyanin (2-3 mg/gf.wt.) from the callus in about 40-60 days under light conditions (continuous light), and (d) Characteristic DNA profile wherein the lanes 1,2,3,4,5,6,7,8,9,10,11 and 12 in each gel (upper gel for wild line and lower gel for anthocyanin producing line) represent the PCR amplified fragments produced by the respective template megabase genomic DNA with primers 5′ CTG ATG CAT C3′, 3′,5′ TGG TCA CTG A 3′, 5′ AGG GGT CTT G 3′,5′ GAA ACG GGT G 3′,5′ AGG GGT CTT G 3′,5′ GCG TAA CGC C 3′,5′ CAG CAC CCA C 3′,5′ GTT GCG ATC C 3′,5′ CAG GCC CTT C 3′,5′ CGC AGT ACT C 3′,5′ GTC CTA CTC G 3′,5′ CTA CAC AGG C 3′ and 5′ GTC CTT AGC G 3′, respectively. Lane M shows the standard size markers of a ladder at 100 bp intervals from 1000 bp downwards.
    • 本发明涉及一种在人参中培养的花青素生产愈伤组织线,其中包括:(a)特征性粉红紫色色素沉淀,(b)约501-80的生长指数约为221-450 培养时间段,(c)在光照条件(连续光照)下,约40-60天从愈伤组织中花青素(2-3mg / gf.wt。)的产量,(d)特征DNA谱,其中泳道1 ,每个凝胶中的2,3,4,5,6,7,8,9,10,11和12(用于野生型线的上凝胶和用于花青素生产线的下凝胶)代表由相应模板megabase产生的PCR扩增片段 基因组DNA与引物5'CTG ATG CAT C3',3',5'TGG TCA CTG A 3',5'AGG GGT CTT G 3',5'GAA ACG GGT G 3',5'AGG GGT CTT G 3' ,5'GCG TAA CGC C 3',5'CAG CAC CCA C 3',5'GTT GCG ATC C 3',5'CAG GCC CTT C 3',5'CGC AGT ACT C 3',5'GTC CTA CTC G 3',5'CTA CAC AGG C 3'和5'GTC CTT AGC G 3'。 泳道M显示从1000bp向下以100bp间隔的梯子的标准尺寸标记。