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    • 7. 发明申请
    • Methods and Materials for Encapsulating Proteins
    • 封装蛋白质的方法和材料
    • US20160075743A1
    • 2016-03-17
    • US14785505
    • 2014-04-17
    • AGRESEARCH LIMITED
    • Jason Nicholas BUSBYJeremy Shaun LOTTMark Robin Holmes HURST
    • C07K14/24C12N15/82C12N9/52A01N37/18
    • The invention provides a method for encapsulating a protein of interest, the method comprising the step of expressing a fusion protein comprising an N-terminal region of a rearrangement hot spot (RHS)-repeat-containing protein fused to the protein of interest. The invention further provides applications for the encapsulation, release and delivery of the protein of interest. The invention also encompasses the encapsulated protein of interest and compositions comprising the encapsulated protein of interest. The invention also provides uses of the encapsulated protein of interest, optionally after release from encapsulation, to control pests. The encapsulated protein of interest may for example be produced via expression in a plant to control a pest of the plant, such as an insect pest.
    • 本发明提供了一种封装目的蛋白质的方法,该方法包括表达融合蛋白的步骤,该融合蛋白包含与目的蛋白质融合的重排热点(RHS) - 含有重复蛋白的蛋白质的N-末端区域。 本发明进一步提供了目的蛋白质的包封,释放和递送的应用。 本发明还包括感兴趣的包封的蛋白质和包含所关注的包封的蛋白质的组合物。 本发明还提供所需的包封的蛋白质,任选在从包囊释放后控制害虫的用途。 所关注的包封的蛋白质可以例如通过在植物中表达来产生,以控制植物的害虫,例如害虫。