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1. 发明授权

US09238222B2 Techniques and droplet actuator designs for reducing bubble formation 有权
标题翻译：用于减少气泡形成的技术和液滴致动器设计
公开(公告)号：US09238222B2
公开(公告)日：2016-01-19
申请号：US14549123
申请日：2014-11-20
申请人： ADVANCED LIQUID LOGIC, INC.
发明人： Cyril Delattre , Arnaud Rival , Vijay Srinivasan
IPC分类号： B01L3/02 , B01L3/00
CPC分类号： B01L3/502784 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/5029 , B01L3/52 , B01L2200/06 , B01L2200/0605 , B01L2300/0645 , B01L2300/0816 , B01L2300/088 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448
摘要： During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.
摘要翻译：在液滴致动器中的液滴操作期间，液滴操作间隙中的气泡通常在填充液中形成并且中断液滴操作。本发明提供了用于在液滴致动器中的液滴上进行液滴操作的方法和系统，包括在液滴操作间隙内对液滴进行多个液滴操作并且/或减少在多个液滴操作期间液滴操作间隙中的电荷。所述方法和系统减少或消除了液滴操作间隙的填充流体中的气泡形成，从而允许完成多个液滴操作，而不会由于液滴操作间隙中的填充流体中的气泡形成而中断。

2. 发明授权

US09132993B1 Load spreader bar pipe connecting sleeve with offset end plate 有权
标题翻译：带偏移端板的吊具杆连接套管
公开(公告)号：US09132993B1
公开(公告)日：2015-09-15
申请号：US14322959
申请日：2014-07-03
申请人： Tandemloc, Inc.
发明人： William DiMartino , Drew Hansen
IPC分类号： B66C1/00 , B66C1/12
CPC分类号： B66C1/12
摘要： A pipe connecting sleeve with offset end plate is disclosed. The pipe connecting sleeve with offset endplate may include a flange having a first side and a second side; first and second insertion parts each including an extension portion extending from their respective sides of the flange each including a distal end, the distal end including an end plate, the end plates being adapted for abutment with an inside diameter of a spreader bar pipe, wherein the end plates are of a greater diameter than the extension portions and are fixed in an offset manner relative thereto; first and second grooves formed on their respective sides of the flange, the grooves being adapted for receiving an end of a spreader bar pipe.
摘要翻译：公开了一种具有偏置端板的管连接套筒。具有偏移端板的管连接套筒可以包括具有第一侧和第二侧的凸缘; 第一和第二插入部分各自包括从凸缘的相应侧面延伸的延伸部分，每个包括远端，该远端包括端板，该端板适于与扩张杆管的内径邻接，其中所述端板的直径大于所述延伸部分的直径，并相对于所述端板以偏移方式固定; 形成在其凸缘的相应侧面上的第一和第二凹槽，所述凹槽适于接纳扩张杆管的一端。

3. 发明授权

US09081007B2 Bead incubation and washing on a droplet actuator 有权
标题翻译：珠子孵化并在液滴致动器上进行洗涤
公开(公告)号：US09081007B2
公开(公告)日：2015-07-14
申请号：US14466193
申请日：2014-08-22
申请人： Advanced Liquid Logic, Inc.
发明人： Michael G. Pollack , Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , G01N33/561 , B01F11/00 , B01F13/00 , B01L3/00 , B03C1/02 , B03C1/30 , B03C5/00 , B01L7/00 , G01N35/10
CPC分类号： G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
摘要翻译：本发明涉及在液滴致动器上的珠孵化和洗涤。提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。

4. 发明授权

US08883513B2 Droplet-based particle sorting 有权
标题翻译：基于液滴的颗粒分选
公开(公告)号：US08883513B2
公开(公告)日：2014-11-11
申请号：US13030418
申请日：2011-02-18
申请人： Michael G. Pollack , Vamsee Pamula , Vijay Srinivasan
发明人： Michael G. Pollack , Vamsee Pamula , Vijay Srinivasan
IPC分类号： G01N1/18 , G01N35/10 , G01N15/00
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
摘要翻译：本发明涉及基于液滴的颗粒分选。根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。

5. 发明授权

US08716015B2 Manipulation of cells on a droplet actuator 有权
标题翻译：操纵液滴致动器上的细胞
公开(公告)号：US08716015B2
公开(公告)日：2014-05-06
申请号：US12334575
申请日：2008-12-15
申请人： Michael G. Pollack , Vamsee K. Pamula , Allen E. Eckhardt
发明人： Michael G. Pollack , Vamsee K. Pamula , Allen E. Eckhardt
IPC分类号： C12N5/00 , C12P19/34 , C12Q1/68 , C12Q1/00 , G01N1/10 , G01N1/00 , G01N35/00
CPC分类号： C12N13/00 , B01J2219/00527 , B01J2219/00653 , B01J2219/00743 , G01N15/10 , G01N35/10 , G01N2015/0088 , G01N2015/1081 , G01N2035/00564 , G01N2035/1034 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： A method of inoculating a culture medium including providing a droplet including a single cell type on a droplet actuator and inoculating a culture medium with the droplet. A method of providing a metabolically useful substance to a cell culture, including providing a droplet actuator including a cell culture droplet loaded thereon, the sample droplet including cells and a cell culture medium, and a second droplet comprising a metabolically useful substance. The method also includes conducting one or more droplet operations to combine the cell culture droplet with the second droplet on the droplet actuator. Related methods, droplet actuators, and systems are also provided.
摘要翻译：一种接种培养基的方法，包括在液滴致动器上提供包含单细胞类型的液滴并用液滴接种培养基。提供代谢有用的物质给细胞培养物的方法，包括提供包括其上装载有细胞培养物液滴的液滴致动器，所述样品液滴包括细胞和细胞培养基，以及包含代谢有用物质的第二液滴。该方法还包括进行一个或多个液滴操作以将细胞培养液滴与液滴致动器上的第二液滴组合。还提供了相关方法，液滴致动器和系统。

6. 发明授权

US08388909B2 Apparatuses and methods for manipulating droplets 有权
公开(公告)号：US08388909B2
公开(公告)日：2013-03-05
申请号：US12576794
申请日：2009-10-09
申请人： Michael G Pollack , Vamsee K Pamula , Richard B Fair
发明人： Michael G Pollack , Vamsee K Pamula , Richard B Fair
IPC分类号： B01L3/00
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.

7. 发明授权

US08349276B2 Apparatuses and methods for manipulating droplets on a printed circuit board 有权
标题翻译：用于操纵印刷电路板上的液滴的装置和方法
公开(公告)号：US08349276B2
公开(公告)日：2013-01-08
申请号：US11343284
申请日：2006-01-30
申请人： Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
发明人： Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
IPC分类号： B01L3/00
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets on a printed circuit board (PCB) are disclosed. Droplets are actuated upon a printed circuit board substrate surface by the application of electrical potentials to electrodes defined on the PCB. The use of soldermask as an electrode insulator for droplet manipulation as well techniques for adapting other traditional PCB layers and materials for droplet-based microfluidics are also disclosed.
摘要翻译：公开了在印刷电路板（PCB）上操纵液滴的装置和方法。通过将电位施加到PCB上限定的电极，使液滴在印刷电路板基板表面上被致动。还公开了使用焊接掩模作为液滴操作的电极绝缘体以及用于适应其它传统PCB层和用于液滴的微流体的材料的技术。

8. 发明授权

US08304253B2 Droplet extraction from a liquid column for on-chip microfluidics 有权
标题翻译：从用于片上微流体的液柱提取液滴
公开(公告)号：US08304253B2
公开(公告)日：2012-11-06
申请号：US12090922
申请日：2006-10-19
申请人： Uichong Brandon Yi , Peter Patrick De Guzman , Wayne Liu , Chang-Jin Kim
发明人： Uichong Brandon Yi , Peter Patrick De Guzman , Wayne Liu , Chang-Jin Kim
IPC分类号： B01D57/00 , B01L99/00
CPC分类号： B01L3/502 , B01L3/502761 , B01L3/502792 , B01L2200/027 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2300/161 , B01L2400/0427 , B01L2400/0469 , G01N1/10 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： A refill droplet facilitates the extraction of a droplet laterally from a channel in a microfluidic apparatus. Such extraction allows a discrete band of separated particles or solute molecules to be excised from a fluid stream and processed and analyzed separately. An extraction point is located along the length of the channel and includes an EWOD surface or similar microfluidic technology to extract a droplet. An opening in the channel opposite the extraction means is equipped with microfluidic technology to transport a refill droplet to the opening. The refill droplet is moved into the channel or column to occupy the area previously occupied by the extracted droplet. This prevents distortion or mixing of the bands of particles or molecules within the channel and prevents the draining of any portion of the fluidic system.
摘要翻译：补充液滴有助于从微流体装置中的通道横向提取液滴。这种提取允许分离的颗粒或溶质分子的离散带从流体流中切除并分开处理和分析。提取点沿着通道的长度定位并且包括用于提取液滴的EWOD表面或类似的微流体技术。与提取装置相对的通道中的开口配备有微流体技术以将再填充液滴输送到开口。再填充液滴移动到通道或柱中以占据先前被提取的液滴占据的区域。这防止了通道内的颗粒或分子带的变形或混合，并且防止流体系统的任何部分的排出。

9. 发明授权

US08268246B2 PCB droplet actuator fabrication 有权
标题翻译： PCB液滴致动器制造
公开(公告)号：US08268246B2
公开(公告)日：2012-09-18
申请号：US12531794
申请日：2008-08-11
申请人： Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack
发明人： Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack
IPC分类号： B01J8/00 , G01N27/26
CPC分类号： H05K3/4038 , B01L3/502707 , B01L3/502784 , B01L3/502792 , B01L2300/089 , B01L2300/12 , B01L2400/0427 , H05K1/113 , H05K3/0058 , H05K3/0067 , H05K3/28 , H05K3/421 , H05K3/427 , H05K2201/09527 , Y10T29/49124 , Y10T29/49155 , Y10T29/49165 , Y10T156/10
摘要： Alternative approaches to fabricating printed circuit boards for use in droplet actuator operations are provided. In one embodiment, a method of manufacturing a droplet actuator for conducting droplet operations includes positioning a dielectric material between a first metal layer configured to include an electrode and a second metal layer configured to include an interconnect pad. The method additionally includes forming a connection between the first and second metal layers. Droplet actuators and methods of fabricating and supporting printed circuit boards of droplet actuators are also provided.
摘要翻译：提供制造用于液滴致动器操作的印刷电路板的替代方法。在一个实施例中，制造用于进行液滴操作的液滴致动器的方法包括将介电材料定位在被配置为包括电极的第一金属层和被配置为包括互连焊盘的第二金属层之间。该方法还包括在第一和第二金属层之间形成连接。还提供了液滴致动器和制造和支持液滴致动器的印刷电路板的方法。

10. 发明授权

US08221605B2 Apparatus for manipulating droplets 有权
标题翻译：用于操纵液滴的装置
公开(公告)号：US08221605B2
公开(公告)日：2012-07-17
申请号：US11965152
申请日：2007-12-27
申请人： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
发明人： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
IPC分类号： F04B19/00 , B81B7/00
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： An apparatus for manipulating droplets is provided. In one embodiment, the apparatus includes a substrate having electrodes and adjacent reference elements configured for manipulating a droplet on a surface of the substrate. Other embodiments having a top plate, a footprint, and a dielectric are also included.
摘要翻译：提供了一种用于操纵液滴的装置。在一个实施例中，该装置包括具有电极和相邻参考元件的基板，该参考元件被配置用于在基板的表面上操纵液滴。还包括具有顶板，覆盖区和电介质的其它实施例。

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