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1. 发明授权

US06989436B2 Stabilization of cardiac troponin I subunits and complexes 失效
公开(公告)号：US06989436B2
公开(公告)日：2006-01-24
申请号：US10358070
申请日：2003-02-04
申请人： Kirti I. Davé , Brian Robert Fernández
发明人： Kirti I. Davé , Brian Robert Fernández
IPC分类号： C07K14/435 , C07K17/02 , C07K17/08 , A61K39/385 , C12Q1/48
CPC分类号： C07K14/4716 , A61K47/60
摘要： The present invention provides methods for preparing, and compositions comprising, stabilized protein-polymer conjugates. More particularly, the present invention relates to the stabilization of individual and complexed subunits of multisubunit protein complexes by conjugation to polymers. Such conjugation acts to stabilize the specific subunit complexes in their native conformation in liquid medium.

2. 发明申请

US20030176638A1 Stabilization of cardiac troponin I subunits and complexes 失效
公开(公告)号：US20030176638A1
公开(公告)日：2003-09-18
申请号：US10358070
申请日：2003-02-04
申请人： Medical Analysis Systems, Inc.
发明人： Kirti I. Dave , Brian Robert Fernandez
IPC分类号： C07K014/47
CPC分类号： C07K14/4716 , A61K47/60
摘要： The present invention provides methods for preparing, and compositions comprising, stabilized protein-polymer conjugates. More particularly, the present invention relates to the stabilization of individual and complexed subunits of multisubunit protein complexes by conjugation to polymers. Such conjugation acts to stabilize the specific subunit complexes in their native conformation in liquid medium.

3. 发明授权

US6114117A Homogeneous diagnostic assay method utilizing simultaneous target and signal amplification 失效
标题翻译：同步诊断测定方法利用同时靶和信号放大
公开(公告)号：US6114117A
公开(公告)日：2000-09-05
申请号：US895495
申请日：1997-07-16
申请人： Jozsef Hepp , Zsolt Lengyel , Rajiv Pande , Janos Botyanszki , Miklos Sahin-Toth
发明人： Jozsef Hepp , Zsolt Lengyel , Rajiv Pande , Janos Botyanszki , Miklos Sahin-Toth
IPC分类号： C12N15/09 , C07K14/745 , C12N9/50 , C12N9/76 , C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/6823 , C12Q1/682 , C12Q2537/119
摘要： The present invention relates to a method, kit and reagent composition for determining the presence of a target nucleic acid in a sample using a two-stage target cycling reaction. During stage 1 of the reaction, the target nucleic acid hybridizes with a probe nucleic acid to effectuate release of an activator into the assay medium. During stage 2 of the reaction, a target analog-anchor complex is cleaved by the activator, which accomplishes the dual purpose of releasing the target analog into the assay medium, and the initiation of signal generation. The released target analog then restarts the cyclic reaction by binding to a second probe, which effectuates release of a second activator, and so on. This cyclic reaction gives rise to an increase in the amount of signal generated from a single target nucleic acid molecule in the sample, which greatly enhances the level of target detection that can be expected.
摘要翻译：本发明涉及一种用于使用两阶段靶向循环反应确定样品中目标核酸的存在的方法，试剂盒和试剂组合物。在反应的阶段1期间，靶核酸与探针核酸杂交以实现激活剂释放到测定培养基中。在反应的第二阶段期间，靶模拟 - 锚配合物被活化剂切割，其实现将目标类似物释放到测定介质中的双重目的，以及信号产生的启动。释放的目标模拟物然后通过结合第二探针重新开始循环反应，这实现了第二活化剂的释放等等。这种循环反应引起样品中单个靶核酸分子产生的信号量的增加，这大大增强了可以预期的目标检测水平。

4. 发明授权

US6072040A Stabilized conjugates of uncomplexed subunits of multimeric proteins 失效
公开(公告)号：US6072040A
公开(公告)日：2000-06-06
申请号：US950925
申请日：1997-10-15
申请人： Kirti I. Dave , Janos Botyanszki , Eva Sintar
发明人： Kirti I. Dave , Janos Botyanszki , Eva Sintar
IPC分类号： A61K47/48 , C07K14/47 , A23J1/00 , A61K39/385
CPC分类号： A61K47/48215 , C07K14/4716 , G01N2333/4712 , Y10S514/97 , Y10S530/81 , Y10S530/815 , Y10S530/841
摘要： The present invention provides methods for preparing, and compositions comprising, stabilized protein-polymer conjugates. More particularly, the present invention relates to the stabilization of individual subunits of multisubunit protein complexes by conjugation to polymers. Such conjugation acts to stabilize the individual subunit in its native conformation in liquid medium, which in turn acts to stabilize its biological activity.

5. 发明授权

US5556788A Fructosmaine reagent and calibrator system 失效
标题翻译：果糖胺试剂和校准器系统
公开(公告)号：US5556788A
公开(公告)日：1996-09-17
申请号：US239610
申请日：1994-05-09
申请人： Shing F. Kwan , Marjorie Bravo-Leerabhandh
发明人： Shing F. Kwan , Marjorie Bravo-Leerabhandh
IPC分类号： G01N33/66 , G01N33/74
CPC分类号： G01N33/66 , Y10S436/825 , Y10S436/904 , Y10T436/144444 , Y10T436/25125
摘要： A system for determining the concentration of fructosamine in sera which consists of a first reagent in which a tetrazolium salt which reduces all reactive substances in sera including fructosamine and a second reagent which is responsive to all reactive substance in sera other than fructosamine. The difference in color change as between the two allows the determination of concentration of fructosamine.
摘要翻译：一种用于测定血清中果糖胺浓度的系统，其由第一试剂组成，其中四唑鎓盐可降低包括果糖胺在内的血清中的所有反应性物质，以及第二试剂，其对除果糖胺以外的血清中的所有反应物质都有反应。两者之间的颜色变化的差异允许确定果糖胺的浓度。

6. 发明授权

US6143537A Method for stabilizing analyte structure using antibodies or antibody fragments thereof 失效
标题翻译：使用抗体或其抗体片段稳定分析物结构的方法
公开(公告)号：US6143537A
公开(公告)日：2000-11-07
申请号：US225905
申请日：1999-01-05
申请人： Shing F. Kwan , Ivan E. Modrovich , Rebecca J. Hunt
发明人： Shing F. Kwan , Ivan E. Modrovich , Rebecca J. Hunt
IPC分类号： G01N33/531 , A61K39/395 , C07K16/40 , C12N9/96 , G01N33/53 , C12Q1/32 , C12Q1/37 , G01N33/573 , C12Q9/96
CPC分类号： C07K16/40 , C12N9/96 , C07K2317/55 , Y10S436/826 , Y10S524/90 , Y10T436/10 , Y10T436/106664 , Y10T436/108331 , Y10T436/2525
摘要： A method for stabilizing analyses with antibodies and antibody fragments comprises dissolving the analyte in a liquid to form a solution, adding analyte-specific antibodies, fragments of such antibodies, or both to the solution, heating the solution, and then cooling and filtering the solution. The filtered solution may be diluted in a suitable matrix.
摘要翻译：用抗体和抗体片段稳定分析的方法包括将分析物溶解在液体中以形成溶液，将分析物特异性抗体，此类抗体的片段或两者加入到溶液中，加热溶液，然后冷却和过滤溶液。过滤的溶液可以在合适的基质中稀释。

7. 发明授权

US5910422A Stable single liquid alpha-amylase reagent 失效
标题翻译：稳定的单液体α-淀粉酶试剂
公开(公告)号：US5910422A
公开(公告)日：1999-06-08
申请号：US700996
申请日：1991-05-10
申请人： Ivan E. Modrovich , Shing S. Kwan , Leon C. Wortham
发明人： Ivan E. Modrovich , Shing S. Kwan , Leon C. Wortham
IPC分类号： C12Q1/34 , C12Q1/40
CPC分类号： C12Q1/40 , C12Q2334/10
摘要： A stable, single liquid alpha-amylase assay reagent comprises a polysaccharide or long chain oligosaccharide substrate for alpha-amylase having an optically detectable label bonded to the reducing end glucose, by a bond cleavable by alpha- or beta-glucosidase, and a blocking group bonded to the terminal end glucose. The reagent further comprises beta-amylase and either alpha- or beta-glucosidase. Sorbitol is provided in an amount of about 5% to retard degradation of the enzymes. Zwitterionic buffers are also provided to stabilize the enzymes. In preparing the reagent, the enzymes and substrate are filtered through a 0.2 micron or less filter to remove alpha-amylase producing organisms.
摘要翻译：稳定的单液体α-淀粉酶测定试剂包含通过α-或β-葡糖苷酶可切割的键和具有与α-还原末端葡萄糖结合的可光学检测标记的α-淀粉酶的多糖或长链寡糖底物和封闭基团与末端葡萄糖结合。试剂还包含β-淀粉酶和α-葡萄糖苷或β-葡糖苷酶。提供山梨糖醇的量为约5％以阻止酶的降解。还提供两性离子缓冲液以稳定酶。在制备试剂时，酶和底物通过0.2微米或更少的过滤器过滤以除去产生α-淀粉酶的生物体。

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