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1. 发明授权

US12077786B2 Methods of cell selection and modifying cell metabolism 有权
公开(公告)号：US12077786B2
公开(公告)日：2024-09-03
申请号：US16966768
申请日：2019-02-01
申请人： LONZA LTD
发明人： Robert Young , Christopher Mark Smales , Colin Mark Jaques , Andrew J. Racher , Gurdeep Singh , James Budge , Joanne Robool
IPC分类号： C12N9/06 , C12N9/02 , C12N15/10 , C12N15/63
CPC分类号： C12N9/0008 , C12N15/1075 , C12N15/63
摘要： Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.

2. 发明公开

US20240151632A1 TEST CHAMBER FOR PHOTOSTABILITY TESTING 审中-公开
公开(公告)号：US20240151632A1
公开(公告)日：2024-05-09
申请号：US18279973
申请日：2022-03-04
申请人： Lonza Ltd
发明人： Lea Sorret , Ahmed Besheer , Maximilian Wozniewski , Martin Vogt
IPC分类号： G01N17/00 , G02B5/28
CPC分类号： G01N17/002 , G01N17/004 , G02B5/283
摘要： The present disclosure provides an ultraviolet light filter for use in photostability testing chambers, and in particular, filters that are designed to allow for air ventilation and maintenance of desired temperatures at the sample level during light exposure testing.

3. 发明公开

US20240132830A1 METHOD AND SYSTEM FOR PROVIDING BUFFER SOLUTIONS 审中-公开
公开(公告)号：US20240132830A1
公开(公告)日：2024-04-25
申请号：US18538702
申请日：2023-12-13
申请人： LONZA LTD
发明人： Tristan WILKINS
IPC分类号： C12M1/34 , C12M1/00 , C12M1/12 , C12M3/00
CPC分类号： C12M41/32 , C12M23/44 , C12M29/00 , C12M37/00 , C12M41/36 , C12M43/00 , B67D7/0294
摘要： A method for delivering a processing solution includes providing a concentrated processing solution in a bioprocess container, the concentrated processing solution being produced at a first site. The method also includes combining the concentrated processing solution with a biopolymer containing solution produced in a bioreactor at a second site different from the first site. In some embodiments, the processing solution is a buffer for processing products of cells cultured in a bioreactor. A system and a pharmaceutical production facility for carrying out the method is also provided.

4. 发明公开

US20230348545A1 HOST CELLS OVEREXPRESSING TRANSLATIONAL FACTORS 审中-公开
公开(公告)号：US20230348545A1
公开(公告)日：2023-11-02
申请号：US18245687
申请日：2021-09-30
申请人： LONZA LTD
发明人： Brigitte GASSER , Jennifer STAUDACHER , Diethard MATTANOVICH
IPC分类号： C07K14/47 , C12N15/79
CPC分类号： C07K14/4705 , C12N15/79
摘要： A recombinant eukaryotic host cell expressing a gene of interest (GOI) which is engineered by genetic modifications to increase expression of two or more genes encoding translation initiation factors (TIF genes) of the messenger ribonucleoprotein (mRNP), compared to the host cell prior to said one or more genetic modifications, wherein said TIF genes comprise at least a gene encoding eIF4A and a gene encoding eIF4G, and wherein expression of at least one of said TIF genes is under transcriptional control of a promoter different from the promoter controlling expression of said GOI.

5. 发明公开

US20230313256A1 MICROBIAL CULTURE MEDIUM 审中-公开
公开(公告)号：US20230313256A1
公开(公告)日：2023-10-05
申请号：US18003991
申请日：2021-07-01
申请人： LONZA LTD
发明人： Nuria ADELANTADO , Hauke HOLM , Christoph KIZIAK
IPC分类号： C12P21/00 , C12N1/20
CPC分类号： C12P21/00 , C12N1/20
摘要： A method of reducing precipitation in a bacterial fermentation process for producing a fermentation product, which fermentation process comprises the steps:

a) cultivating the host cells for bacterial growth in a batch phase using a batch medium; and
b) cultivating the host cells for producing the fermentation product in a fed-batch phase using a feed medium;

which feed and batch media are each unprecipitated media solutions, wherein:

i) the batch medium comprises an amount of calcium salts, magnesium salts, phosphate salts and at least one chelating agent, which is a citrate salt and/or citric acid at an amount of at least 5 mM, and/or EDTA at an amount of at least 1 mM; and
ii) the feed medium comprises an amount of calcium salts which is at least 1 mM, and which is at least 5-fold higher as compared to the batch medium (M/M); and
iii) the feed medium comprises an amount of magnesium salts which is at least 3 mM, and which is at least 2-fold higher as compared to the batch medium (M/M); and
iv) the feed medium comprises an amount of phosphate salts which is less than 90% of the amount comprised in the batch medium (M/M).

6. 发明授权

US11739289B2 Continuous blade impeller 有权
公开(公告)号：US11739289B2
公开(公告)日：2023-08-29
申请号：US16854183
申请日：2020-04-21
申请人： Lonza Limited
发明人： Michael Mietzner , Richard Falk , Rajesh Beri , Mark Caswell
IPC分类号： B01F27/00 , C12M1/06 , B01F27/191 , B01F27/1125 , B01F101/44
CPC分类号： C12M27/02 , B01F27/1125 , B01F27/191 , B01F2101/44
摘要： An impeller is usable in a variable diameter bioreactor having multiple vessel sections of successively increasing or decreasing volume. The impeller includes an impeller blade extending along an impeller blade axis between first and second axial ends and having opposed impeller blade faces, an impeller blade leading edge, and an impeller blade trailing edge. Each of the leading and trailing edges defines a helix or spiral between the axial ends of the impeller blade. In certain arrangements, the impeller blade is one of at least two impeller blades joined together along an impeller shaft extending axially along the impeller blade axis mentioned, and the helix or spiral has a pitch approximately equal to one half of a length between the first and second axial ends.

7. 发明申请

US20230134438A1 DEVICE AND METHOD FOR LEAKAGE TESTING OF A CONNECTION BETWEEN A RUBBER STOPPER AND A CORRESPONDING DRUG CONTAINER 有权
公开(公告)号：US20230134438A1
公开(公告)日：2023-05-04
申请号：US17984769
申请日：2022-11-10
申请人： Lonza Ltd
发明人： Martin Vogt , Roman Mathaes , Hanns-Christian Mahler , Atanas Koulov , Gabriele Roidl , Franziska Riesen Fuchs
IPC分类号： G01M3/22 , G01M3/28
摘要： The invention concerns the field of container closure integrity (CCI) and the testing of CCI and relates to a device and a method for providing a displacement of a rubber stopper for leakage testing of a connection between the rubber stopper and a corresponding drug container which can be used for storing drugs under sterile conditions and which is closed by said rubber stopper.

8. 发明申请

US20230047838A1 MODULATION OF LIPID METABOLISM FOR PROTEIN PRODUCTION 有权
公开(公告)号：US20230047838A1
公开(公告)日：2023-02-16
申请号：US17815465
申请日：2022-07-27
申请人： LONZA LTD
发明人： James BUDGE , Christopher Mark SMALES , Tanya Jeane KNIGHT , Robert YOUNG
IPC分类号： C12N9/02 , C12P21/02 , C07K14/47 , C07K16/00 , C12N5/071
摘要： The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.

9. 发明授权

US11559811B2 Cell culture system and method 有权
公开(公告)号：US11559811B2
公开(公告)日：2023-01-24
申请号：US15891432
申请日：2018-02-08
申请人： Lonza Ltd.
发明人： Jason Bishop , Steven Roberts
IPC分类号： B01L3/00 , C12M1/00 , C12M1/34
摘要： A bioprocess system and a method for incubating, growing and harvesting cell cultures is described. Also disclosed is a bioprocess container that can be used with the system. In one aspect of the present disclosure, the bioprocess system includes bioprocess tubes and cell culture tubes having particular dimensions and being made from specific materials that allow the tubes to be welded together while preventing open connections and/or ruptures. In this manner, bioprocess containers can be connected and disconnected from a cell culture apparatus without having to perform the manipulation within a closed environment and without associated monitoring.

10. 发明授权

US11377865B2 Customizable facility 有权
公开(公告)号：US11377865B2
公开(公告)日：2022-07-05
申请号：US16883505
申请日：2020-05-26
申请人： LONZA LTD
发明人： Leander Biffiger , Sandra Carbonneau , Andre Collioud , Jonathan Fortin , Dominik Imbolden , Torsten Schmidt , Stefan Stoffel , Diego Studer , Christoph Vogel , Stefan Widmer , Tristan Wilkins , Lesley Wood , Alexander Zahiri
IPC分类号： E04H5/02 , E04H5/12 , E04H1/00 , C12M3/00 , B01J19/00 , C12M1/12 , E04B1/00
摘要： A campus for fabricating at least one pharmaceutical product has one or more customizable facilities each configured to manufacture the at least one pharmaceutical product, a media/buffer plant, and a utility building connected by a utility line to the media/buffer plant and/or the one or more customizable facilities to provide at least one first utility to the media/buffer plant and/or the one or more customizable facilities via the utility line.

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