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1. 发明授权

US08012682B2 Nucleic acids encoding chromophores/fluorophores and methods for using the same 有权
标题翻译：编码发色团/荧光团的核酸及其使用方法
公开(公告)号：US08012682B2
公开(公告)日：2011-09-06
申请号：US11607818
申请日：2006-11-30
申请人： Sergey A. Lukyanov , Arcady F. Fradkov , Yulii A. Labas , Mikhail V. Matz , Alexey Terskikh
发明人： Sergey A. Lukyanov , Arcady F. Fradkov , Yulii A. Labas , Mikhail V. Matz , Alexey Terskikh
IPC分类号： C12Q1/68
CPC分类号： C07K14/435 , C07K2319/60
摘要： Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red” (NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green” (NFP-8), and Discosoma sp.“magenta” (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
摘要翻译：提供了编码新色氨酸/荧光素蛋白的核酸组合物及其突变体。感兴趣的主题蛋白质是着色和/或荧光的蛋白质，其中该特征来自蛋白质的两个或多个残基的相互作用。主题蛋白的进一步特征在于它们是从非生物发光的Cnidarian（例如Anthozoan）物种获得的，或者是从非藜芦烷（海洋笔）物种获得的。感兴趣的特异性蛋白质包括从以下特异性Anthozoan物种获得的蛋白质：Anemonia majano（NFP-1），Clavularia sp。（NFP-2），Zoanthus sp。（NFP-3和NFP-4），脊髓细胞瘤（NFP-5），Discosoma sp。（NFP-6），NFP-7（NFP-7），Discosoma sp“green”（NFP-8）和Discosoma sp。“洋红”（NFP-9）。还感兴趣的是与上述特定蛋白质基本相似或突变的蛋白质。还提供了核酸的片段和由其编码的肽，以及针对受试者蛋白质和转基因细胞和生物体的抗体。主题蛋白质和核酸组合物可用于各种不同的应用。最后，提供了用于此类应用的试剂盒，例如包括本发明的核酸组合物。

2. 发明授权

US07258981B2 Sensitive proteasome sensor constructs and methods for their design and use 失效
标题翻译：敏感的蛋白酶体传感器构建及其设计和使用的方法
公开(公告)号：US07258981B2
公开(公告)日：2007-08-21
申请号：US10765244
申请日：2004-01-26
申请人： Pierre H. Turpin , Yu Fang
发明人： Pierre H. Turpin , Yu Fang
IPC分类号： C12Q1/68
CPC分类号： C07H21/04
摘要： Provided are means and methods for sensing proteasome activity and other degradation pathways with a high level of sensitivity.
摘要翻译：提供了用于感测具有高灵敏度水平的蛋白酶体活性和其他降解途径的方法和方法。

3. 发明授权

US07183399B2 Nucleic acids encoding linked chromo/fluorescent domains and methods for using the same 有权
标题翻译：编码相关染色体/荧光结构域的核酸及其使用方法
公开(公告)号：US07183399B2
公开(公告)日：2007-02-27
申请号：US10806930
申请日：2004-03-22
申请人： Sergey Anatolievich Lukyanov
发明人： Sergey Anatolievich Lukyanov
IPC分类号： C07H21/02 , C07K1/00 , G01N33/53
CPC分类号： C07K14/4716 , C07K14/43595 , C07K2319/00
摘要： Nucleic acid compositions encoding polypeptide products having at least two linked chromo/fluorescent domains, as well as the proteins encoded by the same, are provided. Also provided are the polypeptides encoded by the subject nucleic acids, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
摘要翻译：提供编码具有至少两个连接的色素/荧光结构域的多肽产物的核酸组合物以及由其编码的蛋白质。还提供了由对象核酸编码的多肽，以及针对受试者蛋白质和转基因细胞和生物体的抗体。主题蛋白质和核酸组合物可用于各种不同的应用。最后，提供了用于此类应用的试剂盒，例如包括本发明的核酸组合物。

4. 发明授权

US6077673A Mouse arrays and kits comprising the same 失效
标题翻译：鼠标阵列和包含其的试剂盒
公开(公告)号：US6077673A
公开(公告)日：2000-06-20
申请号：US222248
申请日：1998-12-28
申请人： Alex Chenchik , Matvey Lukashev
发明人： Alex Chenchik , Matvey Lukashev
IPC分类号： C12Q1/68 , C40B40/06
CPC分类号： C12Q1/6809 , C12Q1/6837 , B01J2219/00605 , B01J2219/0061 , B01J2219/00659 , B01J2219/00722 , C40B40/06
摘要： Mouse arrays and methods for their use are provided. The subject arrays include a plurality of polynucleotide spots, each of which is made up of a polynucleotide probe composition of unique polynucleotides corresponding to a key mouse gene. The subject arrays find use in hybridization assays, particularly in assays for the identification of differential gene expression of key mouse genes of interest.
摘要翻译：提供了鼠标阵列及其使用方法。受试者阵列包括多个多核苷酸斑点，每个多核苷酸点由对应于关键小鼠基因的独特多核苷酸的多核苷酸探针组合物组成。主题阵列用于杂交测定，特别是用于鉴定感兴趣的关键小鼠基因的差异基因表达的测定中。

5. 发明授权

US5994076A Methods of assaying differential expression 失效
公开(公告)号：US5994076A
公开(公告)日：1999-11-30
申请号：US859998
申请日：1997-05-21
申请人： Alex Chenchik , George Jokhadze , Robert Bibilashvilli
发明人： Alex Chenchik , George Jokhadze , Robert Bibilashvilli
IPC分类号： C12Q1/68 , C40B40/06 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6876 , B01J2219/00605 , B01J2219/0061 , B01J2219/00659 , B01J2219/00722 , C12Q2600/158 , C40B40/06
摘要： Methods and compositions are provided for analyzing differences in the RNA profiles between a plurality of different physiological samples. In the subject methods, a set of a representational number of distinct gene specific primers is used to generate labeled nucleic acids from each of the different physiological samples. The labeled nucleic acids are then compared to each other and differences in the RNA profiles are determined. The subject methods find use in methods of identifying differential gene expression.

6. 发明授权

US5962272A Methods and compositions for full-length cDNA Cloning using a template-switching oligonucleotide 失效
标题翻译：使用模板切换寡核苷酸的全长cDNA克隆的方法和组合物
公开(公告)号：US5962272A
公开(公告)日：1999-10-05
申请号：US778494
申请日：1997-01-03
申请人： Alex Chenchik , York Zhu , Luda Diatchenko , Paul Siebert
发明人： Alex Chenchik , York Zhu , Luda Diatchenko , Paul Siebert
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6855 , C12N15/1096
摘要： The present invention pertains to methods for the synthesis and cloning of full-length cDNA, or cDNA fragments, that correspond to the complete sequence of 5'-ends of mRNA molecules. The method of the present invention comprises contacting RNA with a cDNA synthesis primer which can anneal to RNA, a suitable enzyme which possesses reverse transcriptase activity, and a template switching oligonucleotide under conditions sufficient to permit the template-dependent extension of the primer to generate an mRNA-cDNA hybrid. The template switching oligonucleotide hybridizes to the CAP site at the 5'-end of the RNA molecule and serves as a short, extended template for CAP-dependent extension of the 3'-end of the ss cDNA that is complementary to the template switching oligonucleotide. The resulting full-length ss cDNA includes the complete 5'-end of the RNA molecule as well as the sequence complementary to the template switching oligonucleotide, which can then serve as a universal priming site in subsequent amplification of the cDNA.The subject invention also pertains to the template switching oligonucleotides that can be used according to the subject method. Kits containing the template switching oligonucleotide are also included within the scope of the present invention.
摘要翻译：本发明涉及合成和克隆与mRNA分子的5'末端的完整序列相对应的全长cDNA或cDNA片段的方法。本发明的方法包括使RNA与可以与RNA退火的cDNA合成引物，其具有逆转录酶活性的合适的酶，以及模板切换寡核苷酸，其条件是足以允许引物的模板依赖性延伸以产生 mRNA-cDNA杂交。模板切换寡核苷酸与RNA分子的5'末端的CAP位点杂交，作为与模板切换寡核苷酸互补的ssDNA的3'-末端的CAP依赖性延伸的短扩展模板。所得的全长ss cDNA包括RNA分子的完整5'-末端以及与模板切换寡核苷酸互补的序列，其随后可作为cDNA随后扩增中的通用启动位点。本发明还涉及根据本发明方法可以使用的模板切换寡核苷酸。含有模板切换寡核苷酸的试剂盒也包括在本发明的范围内。

7. 发明授权

US07723125B2 Highly sensitive proteomic analysis methods, and kits and systems for practicing the same 有权
标题翻译：高度敏感的蛋白质组学分析方法，以及实践相同的试剂盒和系统
公开(公告)号：US07723125B2
公开(公告)日：2010-05-25
申请号：US11850474
申请日：2007-09-05
申请人： Grigoriy S. Tchaga
发明人： Grigoriy S. Tchaga
IPC分类号： G01N33/543
CPC分类号： G01N33/552 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , C03C17/30 , C03C17/3405 , G01N33/54353 , G01N33/582 , G01N33/6803 , G01N2400/46
摘要： Methods of determining whether a sample includes one or more analytes, particularly proteinaceous analytes, of interest are provided. In the subject methods, an array of binding agents, where each binding agent includes an epitope binding domain of an antibody, is contacted with the sample. In many embodiments, contact occurs in the presence of a metal ion chelating polysaccharide, e.g., a pectin. Following contact, the presence of binding complexes on the array surface are detected and the resultant data is employed to determine whether the sample includes the one or more analytes of interest. Also provided are kits, systems and other compositions of matter for practicing the subject methods. The subject methods and compositions find use in a variety of applications, including proteomic applications such as protein expression analysis, e.g., differential protein expression profiling.
摘要翻译：提供了确定样品是否包含一种或多种感兴趣的分析物，特别是蛋白质分析物的方法。在本发明方法中，将每种结合剂包括抗体的表位结合结构域的结合剂阵列与样品接触。在许多实施方案中，接触发生在金属离子螯合多糖例如果胶的存在下。接触后，检测阵列表面上结合复合物的存在，并使用得到的数据来确定样品是否包含一种或多种感兴趣的分析物。还提供了用于实践主题方法的试剂盒，系统和其它物质组合物。本发明的方法和组合物可用于各种应用，包括蛋白质组学应用，例如蛋白质表达分析，例如差异蛋白质表达谱。

8. 发明授权

US07166444B2 Nucleic acids encoding chromophores/fluorophores and methods for using the same 有权
标题翻译：编码发色团/荧光团的核酸及其使用方法
公开(公告)号：US07166444B2
公开(公告)日：2007-01-23
申请号：US10006922
申请日：2001-12-04
申请人： Sergey A. Lukyanov , Arcady F. Fradkov , Yulii A. Labas , Mikhail V. Matz , Alexey Terskikh
发明人： Sergey A. Lukyanov , Arcady F. Fradkov , Yulii A. Labas , Mikhail V. Matz , Alexey Terskikh
IPC分类号： C12P21/06
CPC分类号： C07K14/43504 , A01K2217/05 , C07K14/435 , C07K14/43595 , C07K2319/60 , C12N15/65
摘要： Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, as well as the encoded proteins, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species: Anemonia majano (NFP-1), Clavularia sp. (NFP-2), Zoanthus sp. (NFP-3 & NFP-4), Discosoma striata (NFP-5), Discosoma sp. “red”(NFP-6), Anemonia sulcata (NFP-7), Discosoma sp “green” (NFP-8), and Discosoma sp. “magenta” (NFP-9). Also interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
摘要翻译：提供了编码新色氨酸/荧光素蛋白及其突变体的核酸组合物，以及编码的蛋白质。感兴趣的主题蛋白质是着色和/或荧光的蛋白质，其中该特征来自蛋白质的两个或多个残基的相互作用。主题蛋白的进一步特征在于它们是从非生物发光的Cnidarian（例如Anthozoan）物种获得的，或者是从非藜芦烷（海洋笔）物种获得的。感兴趣的特异性蛋白质包括从以下特异性Anthozoan物种获得的蛋白质：Anemonia majano（NFP-1），Clavularia sp。（NFP-2），Zoanthus sp。（NFP-3和NFP-4），脊髓细胞瘤（NFP-5），Discosoma sp。 “NFP-6”，NFP-7，“NFP-7”，“NFP-8”和“Discosoma sp。 “洋红色”（NFP-9）。还感兴趣的是与上述特定蛋白质基本相似或突变的蛋白质。还提供了核酸的片段和由其编码的肽，以及针对受试者蛋白质和转基因细胞和生物体的抗体。主题蛋白质和核酸组合物可用于各种不同的应用。最后，提供了用于此类应用的试剂盒，例如包括本发明的核酸组合物。

9. 发明申请

US20040216180A1 Nucleic acids encoding linked chromo/fluorescent domains and methods for using the same 有权
标题翻译：编码相关染色体/荧光结构域的核酸及其使用方法
公开(公告)号：US20040216180A1
公开(公告)日：2004-10-28
申请号：US10806930
申请日：2004-03-22
申请人： Clontech Laboratories, Inc.
发明人： Sergey Anatolievich Lukyanov
IPC分类号： A01K067/027 , G01N033/53 , C07H021/04 , C07K014/435
CPC分类号： C07K14/4716 , C07K14/43595 , C07K2319/00
摘要： Nucleic acid compositions encoding polypeptide products having at least two linked chromo/fluorescent domains, as well as the proteins encoded by the same, are provided. Also provided are the polypeptides encoded by the subject nucleic acids, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
摘要翻译：提供编码具有至少两个连接的色素/荧光结构域的多肽产物的核酸组合物以及由其编码的蛋白质。还提供了由对象核酸编码的多肽，以及针对受试者蛋白质和转基因细胞和生物体的抗体。主题蛋白质和核酸组合物可用于各种不同的应用。最后，提供了用于此类应用的试剂盒，例如包括本发明的核酸组合物。

10. 发明授权

US6037133A I.kappa.BEGFP constructs, cell lines and methods of use 失效
公开(公告)号：US6037133A
公开(公告)日：2000-03-14
申请号：US062070
申请日：1998-04-17
申请人： Xianqiang Li
发明人： Xianqiang Li
IPC分类号： G01N33/53 , C07K14/435 , C07K14/47 , C07K19/00 , C12N15/09 , C12Q1/02 , G01N33/543 , C07K1/00 , C07K14/00 , C12P21/04 , G01N33/567
CPC分类号： C07K14/47 , C07K14/43595 , C07K2319/60 , C07K2319/71 , C07K2319/80 , G01N2333/43595
摘要： The present invention provides fusion protein comprising I.kappa.B and green fluorescent protein. Also provided is DNA encoding this protein and vector expressing such DNA and various methods of using a fusion protein comprising I.kappa.B and green fluorescent protein.

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