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    • 5. 发明申请
    • METHOD AND SYSTEM FOR WIDE-FIELD MULTI-PHOTON MICROSCOPY HAVING A CONFOCAL EXCITATION PLANE
    • 具有共焦激发平面的宽域多光子显微镜的方法与系统
    • US20080116392A1
    • 2008-05-22
    • US11561766
    • 2006-11-20
    • Gary Brooker
    • Gary Brooker
    • G21K5/04G02B13/14
    • G02B21/0076
    • A wide field microscope includes a stage configured to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source configured to produce excitation light having a single photon energy less than an absorption energy required for single photon excitation of said fluorescent material. A beam expansion unit is optically coupled to the light source and configured to expand the excitation light with reduced pulse spreading characteristics, and an infinity corrected objective optically coupled to the expansion unit and configured to focus the excitation light onto the specimen such that multi-photon excitation of the fluorescent material simultaneously occurs over a predetermined area of the specimen. A focus lens is configured to focus emission light emitted from said predetermined area of the specimen onto at least two pixels of an image detector simultaneously.
    • 宽场显微镜包括配置成保持其中具有荧光材料的样本的台和配置为产生具有小于所述荧光材料的单光子激发所需的吸收能量的单光子能量的激发光的多光子激发光源 。 光束扩展单元光学耦合到光源并且被配置为以减小的脉冲扩展特性来扩展激发光;以及无穷远校正对象,光学耦合到扩展单元并且被配置为将激发光聚焦到样本上,使得多光子 荧光材料的激发同时发生在样品的预定区域上。 焦点透镜被配置为将从样本的所述预定区域发射的发射光同时聚焦到图像检测器的至少两个像素。