专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5424545A Non-invasive non-spectrophotometric infrared measurement of blood analyte concentrations 失效
标题翻译：非侵入性非分光光度法红外测量血液分析物浓度
公开(公告)号：US5424545A
公开(公告)日：1995-06-13
申请号：US182572
申请日：1994-01-14
申请人： Myron J. Block , Lester Sodickson
发明人： Myron J. Block , Lester Sodickson
IPC分类号： A61B5/00 , A61B5/145 , A61B5/1455 , G01N21/31 , G01N21/35 , G01N21/47 , G01N21/61 , G01N33/487
CPC分类号： G01N21/4795 , A61B5/14532 , A61B5/14546 , A61B5/1455 , A61B5/6826 , A61B5/6838 , G01N21/359 , G01N2021/3137
摘要： A new non-invasive non-spectrophotometric method for measuring the blood concentration of analytes such as glucose has been developed. The apparatus and methods of the invention exploit analogies with colorimetry and color perception to extract concentration measurements from the global structure of the intensity versus wavelength absorbance or transmission profile. A plurality of broad spectrum filters transmit distinguishably coded beams of radiation in overlapping portions of the spectrum to the sample. Radiation reflected or transmitted by the sample is detected and decoded. LED's may be used instead of the broad spectrum radiation generating device and the filters. Further, a scanning interferometer can be used as the illuminating and coding device. In a preferred mode, congruent illumination is utilized. The coded signals are analyzed by analogy to colorimetry and visual processing and can be converted into concentration measurements.
摘要翻译：已经开发了用于测量诸如葡萄糖的分析物的血液浓度的新的非侵入性非分光光度法。本发明的装置和方法利用比色和颜色感知的类比从强度对波长吸光度或透射曲线的全局结构提取浓度测量。多个广谱滤波器将光谱的重叠部分中的可分辨地编码的辐射束发射到样品。检测并解码由样本反射或发射的辐射。可以使用LED来代替广谱辐射发生装置和滤光器。此外，扫描干涉仪可以用作照明和编码装置。在优选的模式中，利用了一致的照明。编码信号通过与比色和视觉处理类似地进行分析，并可以转换为浓度测量。

2. 发明授权

US5321265A Non-invasive testing 失效
标题翻译：无创检测
公开(公告)号：US5321265A
公开(公告)日：1994-06-14
申请号：US914265
申请日：1992-07-15
申请人： Myron J. Block
发明人： Myron J. Block
IPC分类号： G01N33/49 , A61B5/00 , G01N21/31 , G01N21/35 , G01N21/47 , G01N33/487
CPC分类号： A61B5/1455 , A61B5/0059 , A61B5/14532 , A61B5/14546 , G01N21/359 , G01N21/4795 , G01N2021/3137
摘要： A new non-invasive detection method for glucose and other constituents of interest in a sample has been developed. The apparatus and methods of the invention provide an analog of color perception of human vision, preferably in the near infrared region, replacing spectrophotometers and narrow band sources used in other noninvasive near infrared detection methods. A plurality of detector units are used, each covering a broad and overlapping region of the detected spectrum, paralleling color perception and colorimetry. In some circumstances, a neural net is used for analysis, allowing the system to learn. A novel method for background discrimination is also described.
摘要翻译：已经开发了用于葡萄糖和样品中感兴趣的其它成分的新的非侵入性检测方法。本发明的装置和方法提供人类视觉的颜色感知的模拟，优选在近红外区域，代替其它非侵入式近红外检测方法中使用的分光光度计和窄带光源。使用多个检测器单元，每个检测器单元覆盖检测到的光谱的宽且重叠的区域，并行色彩感知和比色法。在某些情况下，神经网络用于分析，允许系统学习。还描述了用于背景辨别的新颖方法。

3. 发明授权

US4558014A Assay apparatus and methods 失效
标题翻译：测定仪器和方法
公开(公告)号：US4558014A
公开(公告)日：1985-12-10
申请号：US503500
申请日：1983-06-13
申请人： Tomas B. Hirschfeld , Myron J. Block
发明人： Tomas B. Hirschfeld , Myron J. Block
IPC分类号： G01N21/64 , G01N21/77 , G01N33/53 , G01N33/536 , G01N33/543 , G01N33/58 , G01N21/00
CPC分类号： G01N21/648 , G01N21/7703 , G01N33/5302 , G01N33/54373 , G01N33/582 , Y10S436/80 , Y10S436/807 , Y10S436/808
摘要： An assay apparatus and methods employing total internal fluorescence to define a thin observed volume of a multi-phase suspension from which the suspended phase, because of its size and shape, is substantially excluded. Observations of the fluorescence of a known quantity of fluorescent material introduced into a known volume of the sample containing the observed volume permits quantitation of the volumes of the suspended and suspending phases.
摘要翻译：一种使用全内荧光来定义多相悬浮液的薄观察体积的测定装置和方法，由此基本排除了悬浮相的尺寸和形状。已知量的荧光材料的荧光观察引入含有观察体积的已知体积的样品中，可以定量悬浮和悬浮相的体积。

4. 发明授权

US5242797A Nucleic acid assay method 失效
标题翻译：核酸测定法
公开(公告)号：US5242797A
公开(公告)日：1993-09-07
申请号：US817942
申请日：1992-01-02
申请人： Tomas B. Hirschfeld
发明人： Tomas B. Hirschfeld
IPC分类号： C12Q1/68 , G01N21/77
CPC分类号： G01N21/648 , C12Q1/6825 , G01N21/7703 , G01N21/6428 , Y10T436/143333
摘要： An assay for polynucleotides employing total internal reflection of excitation radiation at a coating bonded to the surface of an optically conductive glass cell. The coating initially includes single-stranded polynucleotides coupled to individual attachment sites on the surface of the cell, such polynucleotides being complementary, at least in part to the single-stranded form of the polynucleotide that is being assayed. Each molecule of the coupled polynucleotide is connected to the cell surface through a spacer connected to an irreversibly conjugated polyadenine/polythymidine sequence at one end of the coupled polynucleotide.When the surface of the coated cell is contacted with a sample that contains single-stranded polynucleotide complementary to the bound polynucleotide, renaturation will occur, forming a double-stranded form of the polynucleotide of interest. A fluorochrome dye specific to that double-stranded form is coupled to the latter, the dye including a chromophore that the excitation radiation will excite into fluorescence. The induced fluorescence is then gathered and measured.
摘要翻译：在与光导玻璃电池的表面结合的涂层上使用激发辐射的全内反射的多核苷酸的测定。涂层最初包括偶联到细胞表面上的各个附着位点的单链多核苷酸，这样的多核苷酸至少部分地是待分析的多核苷酸的单链形式互补的。偶联多核苷酸的每个分子通过连接到偶联多核苷酸一端的不可逆共轭聚腺苷/多胸苷序列的间隔物连接到细胞表面。当涂覆的细胞的表面与含有与结合的多核苷酸互补的单链多核苷酸的样品接触时，会发生复性，形成感兴趣的多核苷酸的双链形式。特异于该双链形式的荧光染料染料与后者结合，染料包括激发辐射将激发成荧光的发色团。然后收集并测量诱导的荧光。

5. 发明授权

US5434412A Non-spectrophotometric measurement of analyte concentrations and optical properties of objects 失效
标题翻译：非分光光度法测量物体的分析物浓度和光学性质
公开(公告)号：US5434412A
公开(公告)日：1995-07-18
申请号：US130257
申请日：1993-10-01
申请人： Lester Sodickson , Myron J. Block
发明人： Lester Sodickson , Myron J. Block
IPC分类号： G01N33/49 , A61B5/00 , A61B5/145 , A61B5/1455 , G01N21/27 , G01N21/31 , G01N21/35 , G01N21/47 , G01N21/64 , G01N33/487
CPC分类号： A61B5/1455 , A61B5/14532 , A61B5/14546 , A61B5/6826 , A61B5/6838 , G01N21/31 , G01N21/359 , G01N21/4795 , G01N2021/3137
摘要： Improvements in non-invasive detection methods for glucose and other constituents of interest in a sample have been developed. The apparatus and methods of the invention provide an analog of color perception of human vision, preferably in the near infrared region, replacing spectrophotometers and narrow band sources used in other non-invasive near infrared detection methods. A plurality of detector units are used, each covering a broad and overlapping region of the detected spectrum, paralleling color perception and colorimetry. The improvements are primarily concerned with improving the signal-to-background (or noise) ratio such that the data stream is improved. These improvements use congruent sampling, comparison of different data streams from different sample portions or filter sets, using an interrogation system with sufficient speed to allow testing of arterial blood, and using a filter with a spectral structure. In some circumstances, a neural net is used for analysis, allowing the system to learn. A novel method for background discrimination is also described.
摘要翻译：已经开发了对样品中葡萄糖和其他感兴趣成分的非侵入性检测方法的改进。本发明的装置和方法提供人类视觉的颜色感觉的模拟，优选在近红外区域，代替其它非侵入式近红外检测方法中使用的分光光度计和窄带光源。使用多个检测器单元，每个检测器单元覆盖检测到的光谱的宽且重叠的区域，并行色彩感知和比色法。这些改进主要涉及改善信号对背景（或噪声）比，使得数据流得到改善。这些改进使用一致的采样，使用具有足够速度的询问系统来对来自不同采样部分或滤波器组的不同数据流进行比较，以允许测试动脉血，并使用具有光谱结构的滤波器。在某些情况下，神经网络用于分析，允许系统学习。还描述了用于背景辨别的新颖方法。

6. 发明授权

US4909990A Immunoassay apparatus 失效
标题翻译：免疫测定仪
公开(公告)号：US4909990A
公开(公告)日：1990-03-20
申请号：US92177
申请日：1987-09-02
申请人： Myron J. Block , Steve J. Lackie , Thomas R. Glass
发明人： Myron J. Block , Steve J. Lackie , Thomas R. Glass
IPC分类号： G01N21/01 , G01N21/64
CPC分类号： G01N21/648 , G01N21/01 , G01N2201/0806 , Y10S435/808 , Y10S436/805 , Y10S436/807 , Y10S436/827
摘要： An assay apparatus employing total internal reflection of excitation radiation at the interface between a replaceable optically conductive rod or fiber and a surrounding liquid phase of lower index of refraction. Immobilized on the surface of the fiber is a component of a complex formed in an immunological-type specific reaction. A fluorophore that can be excited into fluorescence by the excitation radiation is attached to another component of the complex. The rod is coaxially mounted within a tube that is sized with respect to said rod so that a fluid sample may be introduced into said tube.The rod and tubing are supported in a mounting assembly that is attachable to an optical assembly for transmitting excitation radiation into the proximal end of the rod and receiving fluorescent radiation emitted from the proximal end of the rod. Included in the apparatus is a mounting assembly for centering the rod within tube and for biasing the rod in a first direction against an annular seat. The latter is designed to support one end of the rod so that none of the radiation introduced into the rod is intercepted by the seat. The apparatus also includes a seal arrangement for preventing a fluid sample from escaping from the tube. Additionally, a tapered fiber may be included in the optical system of the assay apparatus for increasing the power and numerical aperture of radiation input into the optical rod.
摘要翻译：一种测定装置，其在可替代的光学导电棒或纤维与较低折射率的周围液相之间的界面处采用激发辐射的全内反射。固定在纤维表面上的是在免疫型特异性反应中形成的复合物的成分。可以通过激发辐射激发成荧光的荧光团附着到复合物的另一组分。杆同轴地安装在相对于所述杆的尺寸的管中，使得流体样品可以被引入所述管中。杆和管被支撑在可附接到光学组件的安装组件中，用于将激发辐射传送到杆的近端并接收从杆的近端发射的荧光辐射。该设备中包括一个安装组件，用于将杆定位在管内并用于将杆沿第一方向偏压抵靠环形座。后者被设计成支撑杆的一端，使得没有引入到杆中的辐射被座椅拦截。该装置还包括用于防止流体样品从管中逸出的密封装置。此外，锥形光纤可以包括在测量装置的光学系统中，用于增加输入到光棒中的辐射的功率和数值孔径。

7. 发明授权

US4582809A Apparatus including optical fiber for fluorescence immunoassay 失效
标题翻译：包括用于荧光免疫测定的光纤的装置
公开(公告)号：US4582809A
公开(公告)日：1986-04-15
申请号：US406324
申请日：1982-08-09
申请人： Myron J. Block , Tomas B. Hirschfeld
发明人： Myron J. Block , Tomas B. Hirschfeld
IPC分类号： G01N21/64 , G01N33/543 , G01N33/53 , G01N33/533 , G01N33/552
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/7703 , G01N2021/7786 , Y10S436/805
摘要： A method and apparatus for fluorescent immunoassay utilizes total internal reflection at the interface between a solid phase and a liquid phase of lower index of refraction to produce an evanescent wave in the liquid phase. The solid phase is arranged and illuminated so as to provide multiple total internal reflections at the interface. In a preferred embodiment, the solid phase is in the form of an optical fiber to which is immobilized a component of the complex formed in the immunochemical reaction. A fluorophore is attached to another component of the complex. The fluorescent labelled component may be either the complement to or an analog of the immobilized component, depending on whether competitive or sandwich assays are to be performed. In the case of competitive assays, the labelled component is preferably pre-loaded to the immobilized component in a controlled concentration. The fiber (and the attached constituent of the assay) is immersed in the liquid phase sample. The evanescent wave is used to excite fluorescence in the liquid phase, and that fluorescence which tunnels back into the solid phase and accumulates during multiple reflections while channeled in the solid phase is detected. Diffusional preconcentration onto the reactive surface and double passing enhance the signal and signal/background ratio.
摘要翻译：用于荧光免疫测定的方法和装置利用在较低折射率的固相和液相之间的界面处的全内反射，以在液相中产生ev逝波。固相被布置和照亮，以便在界面处提供多个全内反射。在优选的实施方案中，固相是光纤的形式，其固定化在免疫化学反应中形成的络合物的组分。荧光团连接到复合物的另一组分。荧光标记的组分可以是固定化组分的补体或类似物，这取决于是否进行竞争性或夹心测定法。在竞争性测定的情况下，标记的组分优选以受控浓度预加载到固定化组分。将纤维（和测定的附着成分）浸入液相样品中。消逝波用于激发液相中的荧光，并且检测到在固相中引导时多次反射期间隧道回到固相并累积的荧光。对反应表面的扩散预浓缩和双重通过增强了信号和信号/背景比。

8. 发明授权

US4447546A Fluorescent immunoassay employing optical fiber in capillary tube 失效
标题翻译：在毛细管中使用光纤的荧光免疫测定
公开(公告)号：US4447546A
公开(公告)日：1984-05-08
申请号：US410340
申请日：1982-08-23
申请人： Tomas E. Hirschfeld
发明人： Tomas E. Hirschfeld
IPC分类号： G01N21/76 , G01N21/27 , G01N21/64 , G01N21/77 , G01N21/78 , G01N33/543 , G01N21/00 , G01N33/54 , G01N33/58
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/7703 , G01N33/54373 , G01N2021/0346 , Y10S436/805 , Y10S436/807
摘要： Fluorescent immunoassay apparatus and method employing a disposable consisting, in a preferred embodiment, of a short length of precise diameter capillary tubing having an axially disposed optical fiber to which is immobilized a monolayer of a component of the antibody-antigen complex (e.g., an antibody), an inert diluent, and a preload of a known amount of tagged complement to the immobilized component (e.g., a fluorescent-tagged antigen). The dimensions of the fiber and the capillary tubing are chosen so as to allow the tube to fill itself by capillary action once an end of the tube is immersed into the sample. Precise timing and ballistic measurements may, if desired, be avoided by insuring the incubation time is larger than the diffusion time necessary to scavenge the volume between the fiber and the capillary tubing. Fluorimetric measurement is made by total reflection fluorescence techniques.
摘要翻译：荧光免疫测定装置和方法，在一个优选实施方案中，使用短长度的精确直径的毛细管，其具有轴向设置的光纤，固定有抗体 - 抗原复合物的组分的单层（例如抗体），惰性稀释剂和已知量的标记的补体对固定化组分（例如，荧光标记的抗原）的预加载。选择纤维和毛细管的尺寸，以便一旦将管的一端浸入样品中，管可以通过毛细作用而自身填充。如果需要，可以通过确保孵育时间大于清除纤维和毛细管之间的体积所需的扩散时间来避免精确的时间和弹道测量。通过全反射荧光技术进行荧光测量。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式