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1. 发明申请

US20180112263A1 METHODS FOR DETECTING NUCLEIC ACID SEQUENCE VARIANTS 审中-公开
公开(公告)号：US20180112263A1
公开(公告)日：2018-04-26
申请号：US15796982
申请日：2017-10-30
申请人： Biocept, Inc. , Aegea Biotechnologies
发明人： Lyle ARNOLD
IPC分类号： C12Q1/6869 , C12Q1/6858
摘要： The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.

2. 发明授权

US09671407B2 Devices and methods of cell capture and analysis 有权
公开(公告)号：US09671407B2
公开(公告)日：2017-06-06
申请号：US14223980
申请日：2014-03-24
申请人： Biocept, Inc.
发明人： Stephen D. Mikolajczyk , Tony Pircher , Farideh Z. Bischoff , Pavel Tsinberg
IPC分类号： G01N33/574 , G01N33/543 , G01N33/569 , C12Q1/68
CPC分类号： G01N33/57492 , C12Q1/6886 , C12Q2600/156 , G01N33/54306 , G01N33/56966 , G01N33/57415 , G01N33/57423 , G01N33/57434 , G01N33/57446 , G01N33/57496 , G01N2333/4742 , G01N2333/71
摘要： The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.

3. 发明授权

US09040255B2 Use of diazolidinyl urea for anti-clumping of biological samples 有权
标题翻译：使用二唑烷基脲进行生物样品的抗结块处理
公开(公告)号：US09040255B2
公开(公告)日：2015-05-26
申请号：US13243432
申请日：2011-09-23
申请人： Pavel Tsinberg , Stephen D. Mikolajczyk
发明人： Pavel Tsinberg , Stephen D. Mikolajczyk
IPC分类号： B01L3/00 , G01N1/28 , C12Q1/02 , A61B10/02 , A61B5/15 , A61B5/154 , G01N33/543
CPC分类号： G01N33/54393 , A61B5/1405 , A61B5/150343 , A61B5/150755 , A61B5/154 , A61B10/02 , Y10T436/17 , Y10T436/171538 , Y10T436/173845 , Y10T436/174614 , Y10T436/25 , Y10T436/2525
摘要： The present invention provides methods for preventing clumping of cells in microfluidic devices by addition of diazolidinyl urea (DU). DU can be added to samples at the time of collection or can be added to samples post-collection. DU can also be pre-added to sample collection devices.
摘要翻译：本发明提供了通过加入二唑烷基脲（DU）来防止微流体装置中的细胞结块的方法。 DU可以在收集时添加到样品中，或者可以添加到样品后收集。 DU也可以预先添加到样品收集装置中。

4. 发明授权

US08158410B2 Recovery of rare cells using a microchannel apparatus with patterned posts 有权
标题翻译：使用具有图案柱的微通道装置回收稀有电池
公开(公告)号：US08158410B2
公开(公告)日：2012-04-17
申请号：US11038920
申请日：2005-01-18
申请人： Zhongliang Tang , Ram S. Bhatt , Pavel Tsinberg
发明人： Zhongliang Tang , Ram S. Bhatt , Pavel Tsinberg
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N1/40 , G01N1/405 , Y10T436/25375
摘要： A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
摘要翻译：用于从体液或其他液体样品中分离或分离细胞的微流装置使用流线，其中直线流被横向柱的图案中断。柱在流动路径中的收集区域的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有直线表面，具有弧形横截面，并且被随机排列以便破坏流线型流动。螯合剂如Abs通过亲水涂层，优选含有非常长的异氰酸酯部分或PEG或聚甘氨酸的水凝胶附着在收集区域中的所有表面上，因此在捕获细胞或其它靶向生物分子中是非常有效的这种流线型流动中断。

5. 发明申请

US20100255479A1 DEVICES AND METHODS OF CELL CAPTURE AND ANALYSIS 有权
标题翻译：细胞捕获和分析的设备和方法
公开(公告)号：US20100255479A1
公开(公告)日：2010-10-07
申请号：US12730738
申请日：2010-03-24
申请人： Stephen Mikolajczyk , Tony Pircher , Pavel Tsinberg , Farideh Z. Bischoff
发明人： Stephen Mikolajczyk , Tony Pircher , Pavel Tsinberg , Farideh Z. Bischoff
IPC分类号： G01N33/574 , G01N33/53 , C12M1/34 , C12Q1/68
CPC分类号： G01N33/54306 , G01N33/536 , G01N33/56966 , G01N33/57492
摘要： The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.
摘要翻译：本发明提供了用于从样品，特别是生物样品中分离靶生物分子或细胞的装置。具体地说，该装置包括载体混合物，其含有生物样品和特异性结合靶生物分子或靶细胞的第一结合实体; 以及涂覆有与第一结合实体直接或间接结合的第二结合实体的微通道。还公开了在生物样品中捕获，检测和/或评估靶生物分子或靶细胞（例如癌细胞）的方法。

6. 发明申请

US20070243549A1 ENRICHMENT OF CIRCULATING FETAL DNA 审中-公开
标题翻译：循环FET DNA的扩增
公开(公告)号：US20070243549A1
公开(公告)日：2007-10-18
申请号：US11734224
申请日：2007-04-11
申请人： Farideh Bischoff
发明人： Farideh Bischoff
IPC分类号： C12Q1/68 , C12P19/34 , C07K16/18
CPC分类号： C07K16/18 , C12N15/1003 , C12N15/1006
摘要： A non-invasive screening or diagnostic method for determining the likelihood of a fetus with a genetic abnormality or a potential pregnancy complication, which utilizes a liquid blood sample from a pregnant woman. Antibodies specific to a section of histone 3.1 which is exposed to a far greater extent in chromatin of fetal origin than in chromatin of maternal origin are used to sequester and isolate such fetal nucleosomes including the associated fetal DNA. Following isolation/enrichment of such fetal DNA, genetic analysis is carried out using known molecular diagnostics.
摘要翻译：用于确定具有遗传异常或潜在怀孕并发症的胎儿的可能性的非侵入性筛查或诊断方法，其利用来自孕妇的液体血液样品。组蛋白3.1的一部分特异性抗体，其在胎儿来源的染色质中比在母体来源的染色质中暴露得更大程度地用于隔离和分离包括相关胎儿DNA的这种胎儿核小体。在分离/富集这样的胎儿DNA之后，使用已知的分子诊断进行遗传分析。

7. 发明申请

US20050250111A1 Detection of chromosomal disorders 失效
标题翻译：检测染色体异常
公开(公告)号：US20050250111A1
公开(公告)日：2005-11-10
申请号：US10840208
申请日：2004-05-05
申请人： Zhiyi Xie , Soonkap Hahn , Tim Watanaskul
发明人： Zhiyi Xie , Soonkap Hahn , Tim Watanaskul
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q2537/143 , C12Q2531/113
摘要： Methods for detecting in a single assay any one of multiple chromosomal disorders that result from aneuploidy or certain mutations, particularly microdeletions, and kits for use therein. A polymerase chain reaction (PCR) is carried out to amplify eukaryotic genomic DNA using a plurality of primer oligonucleotide pairs wherein one primer of each pair has a detectable label attached 5′ thereto. A plurality of the primer pairs are targeted to DNA segments of different chromosomes of interest which are indicative of potential chromosomal disorders, and one pair is targeted for a control gene. The amplified PCR products are purified, and single-stranded DNA having the detectable labels is obtained therefrom and hybridized with spots on a microarray that each contain DNA oligonucleotide probes having nucleotide sequences complementary to a nucleotide sequence of one strand of each segment. The microarray is imaged for presence of labels on its respective spots, and the absence or presence of chromosomal disorders as indicated by one or more of the targeted DNA segments of interest is diagnosed by first comparing the imaging results to the imaging of spots specific to the control gene and then to results obtained from imaging normal DNA.
摘要翻译：在单次测定中检测由非整倍体或某些突变，特别是微缺失引起的多种染色体紊乱中的任何一种以及用于其中的试剂盒的方法。进行聚合酶链反应（PCR）以使用多个引物寡核苷酸对扩增真核基因组DNA，其中每对的一个引物具有附着5'的可检测标记。多个引物对靶向不同染色体的DNA片段，其指示潜在的染色体病症，一对靶向对照基因。纯化扩增的PCR产物，从其中获得具有可检测标记的单链DNA，并与微阵列上的斑点杂交，每个点均含有具有与每个片段的一条链的核苷酸序列互补的核苷酸序列的DNA寡核苷酸探针。成像微阵列以在其各自的斑点上存在标记，并且通过首先将成像结果与成像特异性的斑点的成像进行比较来诊断由一个或多个目标DNA片段指示的染色体病症的不存在或存在控制基因，然后从成像正常DNA获得的结果。

8. 发明申请

US20040265883A1 mRNA expression analysis 审中-公开
标题翻译： mRNA表达分析
公开(公告)号：US20040265883A1
公开(公告)日：2004-12-30
申请号：US10851573
申请日：2004-05-20
申请人： Biocept, Inc.
发明人： Tony J. Pircher
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6816 , C12Q1/6813 , C12Q1/6837 , C12Q2545/114 , C12Q2525/173 , C12Q2523/107
摘要： Quantification in a straightforward manner in an mRNA expression analysis is accomplished by using biotinylated oligo d(T) and coupling to a solid support (e.g., streptavidin-derivatized magnetic beads). Labeled targets specific to certain mRNAs of interest that are to be identified and quantified are added to a biological sample containing the mRNAs and biotinylated oligo d(T). The beads are preferably added after hybridization, but may be earlier added. Following attachment to the beads, unbound and non-specifically bound targets and non-mRNA material are removed by successive stringent washings. All the mRNA material is then eluted from the beads by subjection to conditions that separate the poly A and the oligo d(T), and the original mRNAs are then degraded by basic hydrolysis to leave single-strand synthetic targets, which are then hybridized to their specific probes suitably carried on a microarray. The probes and the targets are of synthetic designs, and because all affinity reactions, including hybridization reactions between the targets and the mRNAs, are finalized when the mRNA is bound to the solid substrate, purification of adducts can be performed by simple washings in combination with magnetic separation, avoiding any potentially laborious and time-consuming column separation, filter preparation, centrifugation or preciptitation.

9. 发明授权

US11719692B2 Devices and methods of cell capture and analysis 有权
公开(公告)号：US11719692B2
公开(公告)日：2023-08-08
申请号：US16695976
申请日：2019-11-26
申请人： BIOCEPT, INC.
发明人： Stephen D. Mikolajczyk , Tony Pircher , Pavel Tsinberg , Farideh Z. Bischoff
IPC分类号： G01N33/536 , G01N33/569 , G01N33/543 , G01N33/574
CPC分类号： G01N33/54306 , G01N33/536 , G01N33/56966 , G01N33/56972 , G01N33/57492
摘要： The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.

10. 发明申请

US20120164661A1 METHODS AND REAGENTS FOR SIGNAL AMPLIFICATION 有权
标题翻译：信号放大的方法和试剂
公开(公告)号：US20120164661A1
公开(公告)日：2012-06-28
申请号：US13241083
申请日：2011-09-22
申请人： Stephen D. MIKOLAJCZYK , Lisa S. MILLAR
发明人： Stephen D. MIKOLAJCZYK , Lisa S. MILLAR
IPC分类号： G01N21/64 , G01N33/53 , C07H21/00 , C07K1/04 , C07K17/10 , C08B37/02
CPC分类号： G01N33/58 , C08B37/0021 , C08L5/02 , G01N33/52 , G01N33/533 , G01N33/548 , G01N33/583
摘要： The present invention provides reagents containing binding moieties conjugated to dextran moieties, methods of making such reagents, and use of such reagents in a variety of molecular and cellular assays.
摘要翻译：本发明提供了含有与葡聚糖部分缀合的结合部分的试剂，制备这些试剂的方法，以及在各种分子和细胞测定中使用这些试剂。

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