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1. 发明授权

US07301031B2 Methods for producing hydroxy amino acids, esters, and derivatives thereof 有权
标题翻译：羟基氨基酸，酯及其衍生物的制备方法
公开(公告)号：US07301031B2
公开(公告)日：2007-11-27
申请号：US11014261
申请日：2004-12-16
申请人： J. David Rozzell, Jr. , Spiros Kambourakis
发明人： J. David Rozzell, Jr. , Spiros Kambourakis
IPC分类号： C07C229/00 , C07D211/70 , C07D211/82 , C07D213/55 , C07D333/22
CPC分类号： C07C227/04 , C07D239/42 , C07D263/20 , C07D401/12 , C12N9/0006 , C12P13/04 , C07C229/22 , C07C229/34
摘要： A method for producing a hydroxy-amino ester, or derivative thereof, is provided. A substituted β-ketodiester having a ketone group and two ester functional groups is contacted with a ketoreductase under conditions permitting the reduction of the ketone group to an alcohol. Only one of the ester functional groups is regioselectively hydrolyzed to the corresponding carboxylic acid, whereby a non-hydrolyzed ester functional group remains. The carboxylic acid is converted to an amine or a derivative thereof to produce a hydroxy-amino ester or derivative thereof. A number of novel hydroxy-amino esters are prepared by the method.
摘要翻译：提供了制备羟基 - 氨基酯或其衍生物的方法。具有酮基和2个酯官能团的取代的β-酮糖酵母在酮还原成醇的条件下与酮还原酶接触。只有一个酯官能团被区域选择性地水解成相应的羧酸，由此残留非水解的酯官能团。将羧酸转化为胺或其衍生物以产生羟基 - 氨基酯或其衍生物。通过该方法制备了许多新的羟基 - 氨基酯。

2. 发明申请

US20050100993A1 Methods for producing hydroxy amino acids and derivatives thereof 有权
标题翻译：羟基氨基酸及其衍生物的制备方法
公开(公告)号：US20050100993A1
公开(公告)日：2005-05-12
申请号：US11014423
申请日：2004-12-16
申请人： J. Rozzell , Spiros Kambourakis
发明人： J. Rozzell , Spiros Kambourakis
IPC分类号： C07C227/04 , C07C229/22 , C07C229/34 , C12N9/04 , C12P13/04 , A61K31/195
CPC分类号： C07C227/04 , C12N9/0006 , C12P13/04 , C07C229/22 , C07C229/34
摘要： Hydroxy-amino acids are provided and are prepared by contacting a substituted P-ketodiester having a ketone group and two ester functional groups with a ketoreductase under conditions permitting the reduction of the keytone group to an alcohol. Only one of the ester functional groups is regioselectively hydrolyzed to the corresponding carboxylic acid, whereby a non-hydrolyzed ester functional group remains. The carboxylic acid is converted to an amine to produce a hydroxy-amino acid.
摘要翻译：提供羟基 - 氨基酸，并且通过在允许将酮基团还原成醇的条件下使具有酮基的取代的β-酮酯与酮还原酶与酮还原酶接触来制备。只有一个酯官能团被区域选择性地水解成相应的羧酸，由此残留非水解的酯官能团。将羧酸转化成胺以产生羟基 - 氨基酸。

3. 发明授权

US5885767A Methods and compositions for quantitating L-homocysteine and/or l-methionine in a solution 失效
标题翻译：在溶液中定量L-高半胱氨酸和/或1-甲硫氨酸的方法和组合物
公开(公告)号：US5885767A
公开(公告)日：1999-03-23
申请号：US83459
申请日：1998-05-22
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12Q1/25 , C12Q1/48 , C12Q1/527 , G01N33/68 , C12Q1/00 , C12Q1/37 , C12Q1/54
CPC分类号： C12Q1/25 , C12Q1/48 , C12Q1/527 , G01N33/6815
摘要： A method for quantitating L-homocysteine and/or L-methionine in a solution involves contacting a solution containing L-homocysteine and/or L-methionine with a reagent comprising methionine gamma-lyase and a cofactor capable of forming a Schiff base with the L-methionine and/or L-homocysteine for a time sufficient to catalyze the conversion of L-homocysteine and/or L-methionine to 2-ketobutyrate. The amount of 2-ketobutyrate formed is determined, and the amount of L-homocysteine and/or L-methionine present in the original solution can be determined based on the amount of 2-ketobutyrate formed. A composition for measuring the amount of L-homocysteine and/or L-methionine in a solution comprises methionine gamma-lyase, a cofactor capable of forming a Schiff base with the L-methionine and/or L-homocysteine and at least one 2-ketobutyrate detecting agent, but is substantially free of L-methionine, L-homocysteine, 2-ketobutyrate, pyruvate and mercury.
摘要翻译：在溶液中定量L-高半胱氨酸和/或L-甲硫氨酸的方法包括将含有L-高半胱氨酸和/或L-甲硫氨酸的溶液与含有甲硫氨酸γ-裂合酶的试剂和能够与L形成席夫碱的辅因子接触 - 甲硫氨酸和/或L-高半胱氨酸的时间足以催化L-高半胱氨酸和/或L-甲硫氨酸向2-酮丁酸转化。确定形成的2-酮基丁酸酯的量，并且可以基于形成的2-酮基丁酸酯的量来确定原始溶液中存在的L-高半胱氨酸和/或L-甲硫氨酸的量。用于测量溶液中L-高半胱氨酸和/或L-甲硫氨酸的量的组合物包含甲硫氨酸γ-裂解酶，能够与L-甲硫氨酸和/或L-高半胱氨酸形成希夫碱的辅因子和至少一种2- 酮基丁酸酯检测剂，但基本上不含L-甲硫氨酸，L-高半胱氨酸，2-酮丁酸酯，丙酮酸和汞。

4. 发明授权

US07081535B2 Hydroxy-amino acids 有权
标题翻译：羟基 - 氨基酸
公开(公告)号：US07081535B2
公开(公告)日：2006-07-25
申请号：US11014423
申请日：2004-12-16
申请人： J. David Rozzell, Jr. , Spiros Kambourakis
发明人： J. David Rozzell, Jr. , Spiros Kambourakis
IPC分类号： C07D211/70 , C07D211/82 , C07D213/55 , C07D333/22 , C07C229/00
CPC分类号： C07C227/04 , C12N9/0006 , C12P13/04 , C07C229/22 , C07C229/34
摘要： Hydroxy-amino acids are provided and are prepared by contacting a substituted β-ketodiester having a ketone group and two ester functional groups with a ketoreductase under conditions permitting the reduction of the keytone group to an alcohol. Only one of the ester functional groups is regioselectively hydrolyzed to the corresponding carboxylic acid, whereby a non-hydrolyzed ester functional group remains. The carboxylic acid is converted to an amine to produce a hydroxy-amino acid.
摘要翻译：提供羟基 - 氨基酸，并且通过在允许将酮基团还原成醇的条件下使具有酮基团的取代的β-酮糖酯与酮还原酶与酮还原酶接触来制备。只有一个酯官能团被区域选择性地水解成相应的羧酸，由此残留非水解的酯官能团。将羧酸转化成胺以产生羟基 - 氨基酸。

5. 发明授权

US07202070B2 Method for reductive amination of a ketone using a mutated enzyme 有权
标题翻译：使用突变酶还原胺化酮的方法
公开(公告)号：US07202070B2
公开(公告)日：2007-04-10
申请号：US10039952
申请日：2001-10-24
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12N9/06
CPC分类号： C12Y104/01009 , C12N9/0006 , C12N9/0016 , C12N9/0018 , C12P13/04
摘要： Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amino acid from a target 2-ketoacid, the production of an amine from a target ketone and the production of an alcohol from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination or transamination of the target 2-ketoacid or ketone or the reduction of the ketone and providing the mutated enzyme in a reaction mixture comprising the target 2-ketoacid or ketone under conditions sufficient to permit the formation of the desired amino acid, amine or alcohol to thereby produce the amino acid, amine or alcohol.
摘要翻译：提供了使用突变酶对化合物进行化学转化的方法，更具体地，涉及从靶酮生产氨基酸的方法，从目标酮生产胺和从目标酮生产醇。所述方法包括产生催化目标2-酮酸或酮的还原胺化或转氨酶或还原酮并在包含目标2-酮酸或酮的反应混合物中提供突变酶的突变酶，条件足以允许形成所需的氨基酸，胺或醇，从而产生氨基酸，胺或醇。

6. 发明授权

US5834261A Method for the production of chiral vicinal aminoalcohols 失效
标题翻译：生产手性连位氨基醇的方法
公开(公告)号：US5834261A
公开(公告)日：1998-11-10
申请号：US863900
申请日：1997-05-27
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12P13/00 , C12P41/00
CPC分类号： C12P13/001 , C12P41/002
摘要： The disclosure describes a method for the preparation of chiral vicinal aminoalcohols in high optical purity. The method combines the stereoselective reduction of the keto group of a .beta.-ketoacid, .beta.-keotester, or derivative with the stereospecific rearrangement of the corresponding amide, hyroxamic acid, or hydrazide to produce chiral vicinal aminoalcohols with control of stereochemistry at both chiral centers.
摘要翻译：本公开描述了用于制备高光学纯度的手性连位氨基醇的方法。该方法将β-酮酸，β-酮酯或衍生物的酮基的立体选择性还原与相应的酰胺，氨基酸或酰肼的立体特异性重排相结合，以产生具有控制两个手性中心的立体化学的手性连位氨基醇。

7. 发明申请

US20050196774A1 Synthetic genes for enhanced expression 审中-公开
标题翻译：用于增强表达的合成基因
公开(公告)号：US20050196774A1
公开(公告)日：2005-09-08
申请号：US10989488
申请日：2004-11-15
申请人： J. Rozzell , Peter Bui , Ling Hua
发明人： J. Rozzell , Peter Bui , Ling Hua
IPC分类号： C07K14/21 , C12N15/00 , C12Q1/68 , C07H21/04 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C07K14/21 , C12N15/1089 , C12N15/67
摘要： A synthetic nucleic acid sequence is disclosed, comprising a non-naturally occurring polymer of nucleic acids, having a biological function encoded by the sequence and known from a starting nucleic acid sequence, and having a difference in sequence of at least about 5% between the nucleic acids of the synthetic sequence and the starting sequence. The difference between the nucleic acid sequences results in a different free energy of folding for the synthetic sequence as compared to the starting sequence, such that the synthetic sequence would be expressed better in a selected heterologous host cell than the starting sequence would be if expressed in the same heterologous host cell.
摘要翻译：公开了一种合成核酸序列，其包含非天然存在的核酸聚合物，其具有由该序列编码并且从起始核酸序列已知的生物学功能，并且具有至少约5％之间的序列差异合成序列的核酸和起始序列。与起始序列相比，核酸序列之间的差异导致合成序列的折叠自由能不同，使得合成序列在选择的异源宿主细胞中比起始序列更好地表达，如果在相同的异源宿主细胞。

8. 发明申请

US20080076162A1 Method for chemical transformation using a mutated enzyme 有权
标题翻译：使用突变酶进行化学转化的方法
公开(公告)号：US20080076162A1
公开(公告)日：2008-03-27
申请号：US11786054
申请日：2007-04-09
申请人： J. Rozzell
发明人： J. Rozzell
IPC分类号： C12P13/04 , C12P13/00 , C12P7/02
CPC分类号： C12Y104/01009 , C12N9/0006 , C12N9/0016 , C12N9/0018 , C12P13/04
摘要： Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amino acid from a target 2-ketoacid, the production of an amine from a target ketone and the production of an alcohol from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination or transamination of the target 2-ketoacid or ketone or the reduction of the ketone and providing the mutated enzyme in a reaction mixture comprising the target 2-ketoacid or ketone under conditions sufficient to permit the formation of the desired amino acid, amine or alcohol to thereby produce the amino acid, amine or alcohol.
摘要翻译：提供了使用突变酶对化合物进行化学转化的方法，更具体地，涉及从靶酮生产氨基酸的方法，从目标酮生产胺和从目标酮生产醇。所述方法包括产生催化目标2-酮酸或酮的还原胺化或转氨酶或还原酮并在包含目标2-酮酸或酮的反应混合物中提供突变酶的突变酶，条件足以允许形成所需的氨基酸，胺或醇，由此产生氨基酸，胺或醇。

9. 发明授权

US5942644A Precursors for the production of chiral vicinal aminoalcohols 失效
标题翻译：用于生产手性连位氨基醇的前体
公开(公告)号：US5942644A
公开(公告)日：1999-08-24
申请号：US929474
申请日：1997-09-15
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12P13/00 , C12P41/00 , C07C233/05 , C07C239/08
CPC分类号： C12P13/001 , C12P13/02 , C12P41/002
摘要： The disclosure describes new compositions of matter useful for the preparation of optically-active vicinal aminoalcohols. The compositions are chiral .beta.-hydroxycarboxamides, .beta.-hydroxyhydraxides, and .beta.-hydroxyhydroxamic acids.
摘要翻译：本公开描述了用于制备光学活性连位氨基醇的物质的新组合物。组合物是手性β-羟基甲酰胺，β-羟基氢氧化物和β-羟基异羟肟酸。

10. 发明授权

US5916786A Method for the production of chiral 1,3-aminoalcohols 失效
标题翻译：手性1,3-氨基醇的制备方法
公开(公告)号：US5916786A
公开(公告)日：1999-06-29
申请号：US994163
申请日：1997-12-19
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12P13/00 , C12P7/02
CPC分类号： C12P13/001
摘要： The disclosure describes a method for the preparation of chiral 1,3-aminoalcohols in high optical purity. The method combines the stereoselective oxidation of a 1,4-diol to a gamma-lactone using an alcohol dehydrogenase, the conversion of the gamma-lactone to the corresponding 4-hydroxyamide, 4-hydroxyhydroxamic acid, or 4-hydroxyhydrazide, and stereospecific rearrangement of the 4-hydroxyamide, 4-hydroxyhydroxamic acid, or 4-hydroxyhydrazide to the corresponding chiral 1,3-aminoalcohol.
摘要翻译：本公开描述了制备高光学纯度的手性1,3-氨基醇的方法。该方法将使用醇脱氢酶的1,4-二醇的立体选择性氧化与γ-内酯相结合，将γ-内酯转化为相应的4-羟基酰胺，4-羟基异羟肟酸或4-羟基酰肼，并将立体特异性重排的4-羟基酰胺，4-羟基羟肟酸或4-羟基酰肼与相应的手性1,3-氨基醇反应。

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