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1. 发明授权

US07888053B2 Making encoded ghost cells for multiplexed detection of anti-red cell alloantibodies 有权
标题翻译：制作编码的鬼细胞用于多重检测抗红细胞同种异体抗体
公开(公告)号：US07888053B2
公开(公告)日：2011-02-15
申请号：US12550603
申请日：2009-08-31
申请人： Sukanta Banerjee
发明人： Sukanta Banerjee
IPC分类号： G01N33/00
CPC分类号： G01N33/80 , Y10T436/106664 , Y10T436/13 , Y10T436/2525
摘要： Disclosed are methods for detecting antibody in a sample, where the antibody targets an antigen expressed by red blood cells or red blood cell ghosts. Rather than detecting the binding events between a particular antigen antibody pair (as in traditional agglutination based assays) the methods herein allow for multiplexed detection of clinically important allo-immune antibodies to blood group antigens. Specifically the method involves generating fluorescently encoded red blood cells or red blood cell ghosts with known antigen presentation and using them to detect the presence of antibody in serum/plasma with a fluorescent sandwich type immunoassay. The assay results can be read using flow cytometric or fluorescent microscope based imaging techniques.
摘要翻译：公开了用于检测样品中抗体的方法，其中抗体靶向由红细胞或红细胞重影表达的抗原。不是检测特定抗原抗体对之间的结合事件（如在传统的基于凝集的测定法中），本文的方法允许对血型抗原的临床重要的同种异体免疫抗体的多重检测。具体地说，该方法涉及产生具有已知抗原呈递的荧光编码的红细胞或红血细胞鬼，并使用它们以荧光夹心型免疫测定法检测血清/血浆中抗体的存在。可以使用流式细胞仪或基于荧光显微镜的成像技术读取测定结果。

2. 发明申请

US20100267578A1 PROBE DENSITY SELF-CONSIDERATIONS AND ELONGATION OF COMPLEMENTARY LOOPED PROBES WHERE PROBES ARE ATTACHED TO A SOLID PHASE 有权
标题翻译：探索密度自我考虑和延伸的复杂循环探测器，其中探测器连接到固相
公开(公告)号：US20100267578A1
公开(公告)日：2010-10-21
申请号：US12708362
申请日：2010-02-18
申请人： MICHAEL SEUL , Yi Zhang , Sukanta Banerjee , Jiacheng Yang , Chiu Chau
发明人： MICHAEL SEUL , Yi Zhang , Sukanta Banerjee , Jiacheng Yang , Chiu Chau
IPC分类号： C40B30/04
CPC分类号： C12Q1/6837 , C12Q1/6818 , C12Q1/6827 , C12Q2565/1015 , C12Q2537/143 , C12Q2533/101 , C12Q2535/125 , C12Q2525/301
摘要： In a multiplexed assay method carried out in solution, wherein the solution contains nucleic acid targets and, wherein several different types of oligonucleotide probes, each type having a different sequence in a region designated as a target binding domain, are used to detect the nucleic acid targets, said assay method including a method for increasing the effective concentration of the nucleic acid targets at the surface of a bead to which the oligonucleotide probes are bound, by one or more of the following steps:adjusting assay conditions so as to increase the effective concentration of the targets available for binding to the probes, by one or more of the following: (i) selecting a particular probe density on the surface of the bead; (ii) selecting a solution having an ionic strength greater than a threshold; (ii) selecting a target domain of a size less than a threshold; or (iii) selecting target domains within a specified proximity to a terminal end of the targets.
摘要翻译：在溶液中进行的多重测定方法中，其中溶液含有核酸靶，并且其中使用几个不同类型的寡核苷酸探针，每个类型在被称为靶结合结构域的区域中具有不同序列，以检测核酸目标，所述测定方法包括通过一个或多个以下步骤增加寡核苷酸探针结合珠粒表面上的核酸靶的有效浓度的方法：调节测定条件以增加有效通过一种或多种以下方法可用于结合探针的靶的浓度：（i）在珠的表面上选择特定的探针密度; （ii）选择具有大于阈值的离子强度的溶液; （ii）选择小于阈值的大小的目标域; 或（iii）在目标的终端的指定接近范围内选择目标域。

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