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81. 发明申请

US20150140602A1 METHODS AND MICROBIAL CULTURES FOR IMPROVED CONVERSION OF LIGNOCELLULOSIC BIOMASS 有权
标题翻译：用于改进细菌生物质转化的方法和微生物培养
公开(公告)号：US20150140602A1
公开(公告)日：2015-05-21
申请号：US14412320
申请日：2013-07-05
申请人： DIREVO Industrial Biotechnology GmbH
发明人： Simon Curvers , Vitaly Svetlitchnyi
IPC分类号： C12P7/56 , C12P7/14 , C12P39/00
CPC分类号： C12P7/56 , C12N1/20 , C12N1/22 , C12P7/065 , C12P7/10 , C12P7/14 , C12P7/40 , C12P39/00 , C12P2201/00 , C12R1/01 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The present technology pertains to methods and microbial co-cultures for converting lignocellulosic biomass to biofuels and/or other carbon-based chemicals. Aspects of the present disclosure relate to novel consolidated bioprocessing (CBP) methods by which the efficiency of the production of biofuels and/or other carbon-based chemicals from cellulosic biomass-containing materials can be increased. In particular, the present disclosure provides numerous microbiological co-cultures for increasing the efficiency of ethanol and/or lactic acid production from biomass.
摘要翻译：本技术涉及用于将木质纤维素生物质转化为生物燃料和/或其它碳基化学品的方法和微生物共培养物。本公开的方面涉及新的综合生物处理（CBP）方法，通过该方法可以增加来自含纤维素生物质的材料的生物燃料和/或其他碳基化学物质的生产效率。特别地，本公开提供了许多用于从生物质提高乙醇和/或乳酸生产效率的微生物共培养物。

82. 发明授权

US09023183B2 Processing biomass and petroleum containing materials 有权
标题翻译：加工生物质和含石油的材料
公开(公告)号：US09023183B2
公开(公告)日：2015-05-05
申请号：US13798961
申请日：2013-03-13
申请人： Xyleco, Inc.
发明人： Marshall Medoff
IPC分类号： C10G15/10 , B01J19/08 , C10G11/00 , C10G47/00 , C12P19/14 , C12P7/14 , C12P7/10 , C10G3/00 , C10L1/04 , C08B1/00
CPC分类号： B01J19/08 , B01J19/081 , B01J19/085 , B01J2219/08 , B01J2219/0879 , C08B1/00 , C10G3/00 , C10G11/00 , C10G15/10 , C10G47/00 , C10G2300/1011 , C10L1/02 , C10L1/04 , C10L2200/0469 , C10L2270/02 , C10L2270/04 , C10L2290/02 , C10L2290/06 , C10L2290/26 , C10L2290/34 , C10L2290/36 , C10L2290/543 , C12P5/00 , C12P5/02 , C12P5/023 , C12P7/02 , C12P7/04 , C12P7/10 , C12P7/14 , C12P7/16 , C12P19/02 , C12P19/14 , C12P2201/00 , C12P2203/00 , H01M8/1007 , H01M8/1013 , H01M8/16 , H01M8/22 , Y02E50/16 , Y02E50/343 , Y02P30/20 , Y02T50/678
摘要： Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.
摘要翻译：加工生物质（例如，植物生物量，动物生物质和城市废物生物质）以产生有用的产物，例如燃料。例如，系统可以使用诸如纤维素和/或木质纤维素材料和/或淀粉质材料的原料来生产乙醇和/或丁醇，例如通过发酵。

83. 发明授权

US09012191B2 Dry grind ethanol production process and system with front end milling method 有权
标题翻译：干磨乙醇生产工艺和系统采用前端铣法
公开(公告)号：US09012191B2
公开(公告)日：2015-04-21
申请号：US13428263
申请日：2012-03-23
申请人： Chie Ying Lee
发明人： Chie Ying Lee
IPC分类号： C12P7/06 , C12M1/00 , C12M1/33 , C12P19/02 , C12P19/14 , C12P7/10
CPC分类号： C12P7/14 , C12M21/12 , C12M45/02 , C12M45/04 , C12M45/09 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , C12P2203/00 , Y02E50/16 , Y02E50/17
摘要： A dry grind ethanol production process and system with front end milling method is provided for improving alcohol and/or by-product yields, such as oil and/or protein yields. In one example, the process includes grinding corn kernels into particles then mixing the corn particles with a liquid to produce a slurry including oil, protein, starch, fiber, germ, and grit. Thereafter, the slurry is subjected to a front end milling method, which includes separating the slurry into a solids portion, including fiber, grit, and germ, and a liquid portion, including oil, protein, and starch, then milling the separated solids portion to reduce the size of the germ and grit and release bound starch, oil, and protein from the solids portion. The starch is converted to sugar, and alcohol is produced therefrom then recovered. Also, the fiber can be separated and recovered. Oil and protein may be separated and recovered as well.
摘要翻译：提供用于提高醇和/或副产物产率（例如油和/或蛋白质产率）的干研磨乙醇生产方法和具有前端研磨方法的系统。在一个实例中，该方法包括将玉米颗粒研磨成颗粒，然后将玉米颗粒与液体混合以产生包括油，蛋白质，淀粉，纤维，胚芽和砂砾的浆料。此后，对浆料进行前端研磨方法，其包括将浆料分离成包括纤维，砂砾和胚芽的固体部分和包括油，蛋白质和淀粉在内的液体部分，然后研磨分离的固体部分以减少胚芽和砂粒的大小并从固体部分释放结合的淀粉，油和蛋白质。将淀粉转化成糖，然后回收醇。此外，纤维可以分离和回收。油和蛋白质也可以分离和回收。

84. 发明授权

US09005948B2 EGVII endoglucanase and nucleic acids encoding the same 有权
公开(公告)号：US09005948B2
公开(公告)日：2015-04-14
申请号：US14159044
申请日：2014-01-20
申请人： Danisco US Inc.
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
IPC分类号： C12N9/42 , C12P7/06 , C12P21/04 , C12P21/06 , C12N15/00 , C12N1/20 , C07H21/04 , C11D3/38 , C11D3/386 , C12P7/10 , C12P7/14
CPC分类号： C12N9/2437 , C11D3/38 , C11D3/38636 , C11D3/38645 , C12N9/2445 , C12N15/1137 , C12N2310/11 , C12P7/10 , C12P7/14 , C12P19/02 , C12P19/14 , C12P2201/00 , C12Y302/01004 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.

85. 发明申请

US20150099284A1 Alpha-Amylase Mutants with Altered Properties 审中-公开
标题翻译：具有改变特性的α-淀粉酶突变体
公开(公告)号：US20150099284A1
公开(公告)日：2015-04-09
申请号：US14572409
申请日：2014-12-16
申请人： NOVOZYMES A/S
发明人： Thomas Thisted , Soren Kjaerulff , Carsten Andersen , Claus Crone Fuglsang
IPC分类号： C12N9/28 , D06M16/00 , C12P19/02 , C11D3/386 , C12P7/14 , C12P19/14
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C11D3/38681 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
摘要翻译：本发明涉及母体类戊酰样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且表现出改变的稳定性，特别是在高温和/或低pH相对和/或低Ca 2+下亲本α-淀粉酶。

86. 发明授权

US08999696B2 Compositions and methods for enhancing the degradation or conversion of cellulose-containing material 有权
标题翻译：用于增强含纤维素材料的降解或转化的组合物和方法
公开(公告)号：US08999696B2
公开(公告)日：2015-04-07
申请号：US12598574
申请日：2008-05-09
申请人： Keith McFarland
发明人： Keith McFarland
IPC分类号： C12N9/42 , C12P7/06
CPC分类号： C12P13/20 , C12N9/2437 , C12N9/2445 , C12P5/023 , C12P7/06 , C12P7/14 , C12P7/24 , C12P7/26 , C12P19/14 , C12P2201/00 , C12P2203/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17 , Y02E50/343 , Y02P20/52
摘要： The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.
摘要翻译：本发明涉及降解或转化含纤维素材料的方法，其包括：用有效量的包含具有纤维素分解增强活性的多肽的纤维素分解酶组合物和选择的一种或多种（多种）组分处理含纤维素材料由具有内切葡聚糖酶活性的CEL7多肽，具有内切葡聚糖酶活性的CEL12多肽，具有内切葡聚糖酶活性的CEL45多肽，具有纤维素结合结构域的纤维二糖水解酶活性的CEL7多肽和不含纤维素结合结构域的纤维二糖水解酶活性的CEL7多肽。本发明还涉及这种纤维素分解酶组合物。

87. 发明授权

US08993288B2 Method for converting biomass to methane 有权
标题翻译：将生物质转化为甲烷的方法
公开(公告)号：US08993288B2
公开(公告)日：2015-03-31
申请号：US14031758
申请日：2013-09-19
申请人： Anaergia Inc.
发明人： F. Michael Lewis
IPC分类号： C12P7/10 , C10B47/44 , C10B53/02 , C10C5/00 , C10L3/08 , C12P5/02 , C12P7/08 , C12P7/14
CPC分类号： C12P7/08 , C10B47/44 , C10B53/02 , C10C5/00 , C10J3/007 , C10J2200/158 , C10J2300/0946 , C10J2300/1681 , C10L3/08 , C12M21/12 , C12M29/00 , C12M43/00 , C12P5/023 , C12P7/10 , C12P7/14 , Y02E50/14 , Y02E50/16 , Y02E50/32 , Y02E50/343
摘要： A method for enhancing the treatment of lignocellulose-containing materials by biotreatment wherein such lignocellulose-containing materials, normally resistant to biotreatment, are first subjected to a low-temperature, long-residence time pyrolysis at about 175° C. to about 325° C. for about 0.1 hour to about 2.0 hours, wherein a substantial portion of the incoming material is distilled into water-soluble compounds amenable to anaerobic biotreatment. Exemplary applications of the method include pyrolytic pre-treatment of wastewater sludges, cellulosic wastes, wood, peat, plant residues, low-grade coal, and the like to enhance methane gas production in anaerobic digestion and/or oxygen-limited or oxygen-starved fermentation to produce ethanol.
摘要翻译：通过生物处理增强对含木素纤维素材料的处理的方法，其中通常对生物处理有抗性的这种含木素纤维素的材料首先在约175℃至约325℃下进行低温，长停留时间热解约0.1小时至约2.0小时，其中大部分进料进料被蒸馏成易于厌氧生物处理的水溶性化合物。该方法的示例性应用包括废水淤泥，纤维素废物，木材，泥炭，植物残渣，低等级煤等的热解预处理，以增强厌氧消化和/或氧限制或氧饥饿中的甲烷气体产生发酵生产乙醇。

88. 发明授权

US08993286B2 Methods for enhancing the degradation of cellulosic material with chitin binding proteins 有权
公开(公告)号：US08993286B2
公开(公告)日：2015-03-31
申请号：US14117317
申请日：2012-05-18
申请人： Feng Xu
发明人： Feng Xu
IPC分类号： C12P7/06 , C12P7/14 , C12P7/10 , C12P19/02 , C12P19/04 , C12P19/14 , C07K14/32 , C12N1/22 , C12N9/42
CPC分类号： C12P19/14 , C07K14/32 , C12N1/22 , C12N9/2442 , C12P7/04 , C12P7/10 , C12P7/14 , C12P19/02 , C12P19/04 , C12P19/12 , C12Y302/01014 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material.

89. 发明授权

US08986969B2 Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same 有权
公开(公告)号：US08986969B2
公开(公告)日：2015-03-24
申请号：US14064457
申请日：2013-10-28
申请人： Novozymes A/S
发明人： Lene Lange , Wenping Wu , Dominique Aubert , Sara Landvik , Kirk Matthew Schnorr , Ib Groth Clausen
IPC分类号： C12N9/14 , C12P1/00 , C12P21/04 , A61K39/00 , C12N9/42 , C11D3/386 , C12P7/14
CPC分类号： C12N9/2437 , C11D3/386 , C11D3/38645 , C12N9/2408 , C12N9/2477 , C12P7/14 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.

90. 发明授权

US08975056B2 Variant alpha-amylases from Bacillus subtilis and methods of uses, thereof 有权
标题翻译：来自枯草芽孢杆菌的变体α-淀粉酶及其使用方法
公开(公告)号：US08975056B2
公开(公告)日：2015-03-10
申请号：US13660938
申请日：2012-10-25
申请人： Danisco US Inc.
发明人： Luis G. Cascao-Pereira , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Scott D. Power , Sandra W. Ramer , Amr Toppozada , Louise Wallace
IPC分类号： C12N9/28 , C12N9/34 , C12P19/14 , C12P19/20 , C07H21/04 , C07K1/00 , C11D3/00 , C11D1/00 , C12N9/26 , G01N11/00
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
摘要： Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
摘要翻译：提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。

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