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81. 发明授权

US08263824B2 Polypeptides having lipase activity and polynucleotides encoding same 有权
标题翻译：具有脂肪酶活性的多肽和编码该多肽的多核苷酸
公开(公告)号：US08263824B2
公开(公告)日：2012-09-11
申请号：US13440626
申请日：2012-04-05
申请人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
发明人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC分类号： A01H1/00 , C12N9/10 , C12N15/00 , C07H21/02
CPC分类号： C12N9/20
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有脂肪酶活性的分离的多肽和编码多肽的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。

82. 发明申请

US20120190088A1 METHOD OF PRODUCING FATTY ACID ALKYL ESTER USING MICROORGANISMS HAVING ABILITY TO PRODUCE OIL 有权
标题翻译：使用具有生产油的能力的微生物生产脂肪酸的方法
公开(公告)号：US20120190088A1
公开(公告)日：2012-07-26
申请号：US13384576
申请日：2010-07-19
申请人： Sang Yup Lee , Yong Jun Choi
发明人： Sang Yup Lee , Yong Jun Choi
IPC分类号： C12P7/64
CPC分类号： C12N9/20 , C12P7/649 , C12Y301/01003 , Y02E50/13 , Y02P20/52
摘要： The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel.
摘要翻译：本发明涉及使用具有生产油的能力的微生物生产脂肪酸烷基酯的方法，更具体地说，涉及生产脂肪酸烷基酯的方法，该方法包括培养具有生产油的微生物，从而在微生物中积聚大量的油，引起微生物中产生的油的自溶以产生游离脂肪酸，并将游离脂肪酸转化为烷基酯。根据本发明的方法，可以使用代谢工程方法将积累在微生物中的微生物油（例如由微生物产生的典型油的三酰基甘油）的油转化为高效率的脂肪酸烷基酯。因此，本发明的方法可用于最近被发现作为生物柴油有效的脂肪酸烷基酯的工业生产。

83. 发明申请

US20120190067A1 Promoter Variants For Expressing Genes In A Fungal Cell 有权
标题翻译：在真菌细胞中表达基因的启动子变体
公开(公告)号：US20120190067A1
公开(公告)日：2012-07-26
申请号：US13424018
申请日：2012-03-19
申请人： Debbie Yaver , Peter Nham
发明人： Debbie Yaver , Peter Nham
IPC分类号： C12P21/00 , C12N1/19 , C12N15/63 , C12N1/15 , C12N9/00 , C12N15/113
CPC分类号： C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/58 , C12N9/93 , C12N15/113 , C12P1/02 , C12P21/00 , C12Y107/01003 , C12Y301/01003 , C12Y304/21004 , C12Y603/04002
摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NOs: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.
摘要翻译：本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列与包含选自SEQ ID NO：2,3,4,5,6,7,8,9,10,11和12的启动子变体的第二核酸序列连接; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。

84. 发明授权

US08227215B2 Hydrolases, nucleic acids encoding them and methods for making and using them for biocatalytic synthesis of a structured lipid 有权
标题翻译：水解酶，编码它们的核酸和制备和使用它们用于结构化脂质的生物催化合成的方法
公开(公告)号：US08227215B2
公开(公告)日：2012-07-24
申请号：US13083455
申请日：2011-04-08
申请人： Christopher L. G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
发明人： Christopher L. G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
IPC分类号： C12P7/00 , C12P21/04 , C12P21/06 , C12Q1/68 , C12Q1/34 , C12Q1/00 , C12N9/14 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/6472 , A23D7/04 , A23D9/04 , C11B3/003 , C11C1/045 , C11C3/08 , C11C3/12 , C12N9/20 , C12P7/6418 , C12P7/6427 , C12P7/6445 , C12P7/6454
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
摘要翻译：提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法，例如，大豆油或卡诺拉油。

85. 发明申请

US20120171736A1 POWDERY LIPASE PREPARATION AND USE THEREOF 审中-公开
标题翻译：粉末脂肪酶的制备和使用
公开(公告)号：US20120171736A1
公开(公告)日：2012-07-05
申请号：US13395607
申请日：2010-11-26
申请人： Satoshi Negishi , Yoshie Yamauchi , Yosuke Nakamura , Yuko Toyama
发明人： Satoshi Negishi , Yoshie Yamauchi , Yosuke Nakamura , Yuko Toyama
IPC分类号： C12P7/64 , B32B5/16 , C12N9/20
CPC分类号： C12N9/20 , C12N9/96 , C12P7/6436 , C12P7/6454 , Y10T428/2982
摘要： The present invention discloses a powdery lipase preparation in which the particles constituting the powdery lipase have 3,000 to 40,000 pores/mm2 on the surface thereof, and each pore has a diameter of 0.5 μm to 6 μm. This powdery lipase preparation was able to improve lipase activity without using a soybean powder.
摘要翻译：本发明公开了一种粉末状脂肪酶制剂，其中构成粉状脂肪酶的颗粒在其表面上具有3,000至40,000个孔/ mm 2，并且每个孔的直径为0.5μm至6μm。该粉状脂肪酶制剂能够在不使用大豆粉的情况下改善脂肪酶活性。

86. 发明申请

US20120164701A1 RENEWABLE CHEMICALS AND FUELS FROM OLEAGINOUS YEAST 审中-公开
标题翻译：来自OLEAGOOUS YEAST的可再生化学品和燃料
公开(公告)号：US20120164701A1
公开(公告)日：2012-06-28
申请号：US13406417
申请日：2012-02-27
申请人： Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
发明人： Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
IPC分类号： C10L1/16 , C12P5/02 , C07C69/24 , C12P7/64
CPC分类号： C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
摘要： The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.
摘要翻译：本发明提供了使用单独的木糖或与其它解聚的纤维素材料组合培养含油微生物的方法。还提供了包含编码多糖降解酶的外源基因如纤维素酶，半纤维素酶，果胶酶或者碎裂酶的微生物。提供了微生物发酵的一些方法，其包括使用木糖和解聚的纤维素材料来生产含油微生物。

87. 发明授权

US08203031B2 Transgenic plants exhibiting increased resistance to biotic and abiotic stresses and methods for producing the same 有权
标题翻译：表现出对生物和非生物胁迫或加速开花时间的抗性增加的转基因植物及其生产方法
公开(公告)号：US08203031B2
公开(公告)日：2012-06-19
申请号：US12527323
申请日：2008-02-15
申请人： Stephen Beungtae Ryu , Hyoung-Yool Lee , In-Whan Hwang , P. Palta Jiwan
发明人： Stephen Beungtae Ryu , Hyoung-Yool Lee , In-Whan Hwang , P. Palta Jiwan
IPC分类号： A01H3/00 , A01H5/00
CPC分类号： C12N15/8282 , C12N9/20 , C12N15/8255 , C12N15/8273 , C12N15/8281
摘要： The invention relates to plants, especially transgenic plants, plant parts and plant cells overproducing a secretory phospholipase A2 protein (e.g. sPLA2-a or sPLA2-b) and having an enhanced resistance against a wide range of abiotic (e.g., against osmotic stress) and biotic stress (e.g., bacterial, or fungal infections) conditions as well as inducing early flowering. The invention also comprises nucleic acid sequences encoding a secretory phospholipase A2 or functional isoforms thereof and the use of such sequences for rendering plants resistant against abiotic and biotic stress conditions. The invention is useful for mitigating crop damages by a wide variety of pathogen infections and stress conditions and for accelerating flowering time.
摘要翻译：本发明涉及植物，特别是转基因植物，过量产生分泌性磷脂酶A2蛋白（例如sPLA2-a或sPLA2-b）并且具有对广泛范围的非生物（例如抗渗透胁迫）的增强的抗性的植物部分和植物细胞，以及生物胁迫（例如细菌或真菌感染）条件以及诱导早期开花。本发明还包括编码分泌性磷脂酶A2或其功能异构体的核酸序列，以及这些序列用于提高抗非生物和生物胁迫条件的植物的用途。本发明可用于通过各种病原体感染和胁迫条件减轻作物损害并用于加速开花时间。

88. 发明授权

US08192782B2 Enzymatic oil-degumming method 有权
标题翻译：酶法脱脂方法
公开(公告)号：US08192782B2
公开(公告)日：2012-06-05
申请号：US11623689
申请日：2007-01-16
申请人： Jorn Borch Søe , Mark Turner
发明人： Jorn Borch Søe , Mark Turner
IPC分类号： A23D7/00 , C12N9/00 , C12N9/10 , C12N15/00 , C07H21/04
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： A process of enzymatic degumming edible oils, comprising treating edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxyl group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprise one or more of the following amino acid sequences: SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID NO: 16 is also taught.
摘要翻译：一种酶促脱脂食用油的方法，包括用脂质酰基转移酶处理食用油，以将酰基从大部分磷脂转移到一个或多个酰基受体上，其中酰基受体可以是包含羟基的任何化合物。在一个实施方案中，优选地，酰基受体是水，在另一个实施方案中，优选酰基受体是一种或多种甾醇和/或甾烷醇。当酰基受体是甾烷醇和/或甾醇时，产生一种或多种甾醇酯和/或甾烷醇酯。用于本发明方法的脂质酰基转移酶可以包含一个或多个以下氨基酸序列：SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO： SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11，SEQ ID NO：12，SEQ ID NO：13， 14，或SEQ ID NO：15，SEQ ID NO：16，SEQ ID NO：17，SEQ ID NO：18，SEQ ID NO：36，SEQ ID NO：38，SEQ ID NO：40，SEQ ID NO：41 ，SEQ ID NO：45，SEQ ID NO：47，SEQ ID NO：50或与其同一性为75％以上的氨基酸序列。还教导了包含SEQ ID NO：16所示氨基酸序列的新型脂质酰基转移酶。

89. 发明授权

US08187854B2 Lipase variants 有权
标题翻译：脂肪酶变体
公开(公告)号：US08187854B2
公开(公告)日：2012-05-29
申请号：US12161582
申请日：2007-01-22
申请人： Jesper Vind , Jürgen Carsten Franz Knötzel , Kim Borch , Allan Svendsen , Thomas Honger Callisen , Debbie Yaver , Mads Eskelund Bjornvad , Peter Kamp Hansen , Haiyan Ge , Michael Lamsa
发明人： Jesper Vind , Jürgen Carsten Franz Knötzel , Kim Borch , Allan Svendsen , Thomas Honger Callisen , Debbie Yaver , Mads Eskelund Bjornvad , Peter Kamp Hansen , Haiyan Ge , Michael Lamsa
IPC分类号： C12N9/20 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/20 , C12N9/18
摘要： The invention provides variant lipases, preferably, variants with reduced tendency to odor generation obtained by introducing mutations in one or more regions identified in a parent lipase.
摘要翻译：本发明提供了变体脂肪酶，优选具有通过在母体脂肪酶中鉴定的一个或多个区域中引入突变获得的具有降低的气味产生倾向的变体。

90. 发明授权

US08153391B2 Hydrolases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：水解酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US08153391B2
公开(公告)日：2012-04-10
申请号：US12202204
申请日：2008-08-29
申请人： Christopher L. G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
发明人： Christopher L. G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
IPC分类号： C12Q1/34 , C12Q1/00 , C12N9/14 , C12N1/20 , C12N15/00 , C12P7/00 , C12P21/04 , C12P21/06 , C07H21/04
CPC分类号： C12P7/6472 , A23D7/04 , A23D9/04 , C11B3/003 , C11C1/045 , C11C3/08 , C11C3/12 , C12N9/20 , C12P7/6418 , C12P7/6427 , C12P7/6445 , C12P7/6454
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
摘要翻译：提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法，例如，大豆油或卡诺拉油。

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