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81. 发明申请

US20060037086A1 Methods for correcting mitotic spindle defects and optimizing preimplantation embryonic developmental rates associated with somatic cell nuclear transfer in animals 审中-公开
标题翻译：纠正有丝分裂纺锤体缺陷和优化与动物体细胞核转移相关的植入前胚胎发育率的方法
公开(公告)号：US20060037086A1
公开(公告)日：2006-02-16
申请号：US11003006
申请日：2004-12-03
申请人： Gerald Schatten , Calvin Simerly , Christopher Navara
发明人： Gerald Schatten , Calvin Simerly , Christopher Navara
IPC分类号： A01K67/00
CPC分类号： A01K67/0273 , A01K2227/106 , C12N5/0604 , C12N5/0609 , C12N15/877 , C12N15/8776 , C12N2517/04
摘要： The present invention is directed to various methodologies to make NT a practical procedure for animals, specifically, primates including human and non-human primates. Furthermore, the methods and molecular components provided by the present invention provide a practical means for producing embryos with desired characteristics. In a specific embodiment, the methodology of the present invention comprises introducing nuclei having desired characteristics along with one or more molecular components into an enucleated egg, thus creating a nuclear transfer construct, culturing the egg to produce a viable embryo, transferring the embryo to the oviducts of a female, and producing a cloned animal.
摘要翻译：本发明涉及使NT成为动物，特别是包括人和非人灵长类动物的灵长类动物的实用程序的各种方法。此外，本发明提供的方法和分子组分提供了生产具有所需特征的胚胎的实用手段。在具体实施方案中，本发明的方法包括将具有所需特征的核以及一种或多种分子成分引入去核卵中，从而产生核转移构建体，培养卵以产生活的胚胎，将胚胎转移至雌性输卵管，并产生克隆的动物。

82. 发明申请

US20050186545A1 Method of constructing spinal injury model monkey and utilization thereof 有权
标题翻译：构建脊髓损伤模型猴的方法及其应用
公开(公告)号：US20050186545A1
公开(公告)日：2005-08-25
申请号：US10496993
申请日：2002-11-26
申请人： Hideyuki Okano , Yoshiaki Toyama , Masaya Nakamura , Tatsuji Nomura , Yoshikuni Tanioka , Kiyoshi Ando , Yonehiro Kanemura
发明人： Hideyuki Okano , Yoshiaki Toyama , Masaya Nakamura , Tatsuji Nomura , Yoshikuni Tanioka , Kiyoshi Ando , Yonehiro Kanemura
IPC分类号： A01K67/027 , A61P25/00 , C12N15/85 , G09B23/28
CPC分类号： A01K67/027 , A01K2227/106 , A01K2267/03 , A01K2267/0356
摘要： A method for creating a monkey model of spinal cord injury, which includes exposing the dura mater of the cervical cord of a monkey and applying a load on the dura mater; the thus-created monkey model of spinal cord injury; and a method for evaluating a therapeutic drug for spinal cord injury by use of this model. According to the present invention, it is possible to create a monkey which is close to the human and thus useful as a model of human spinal cord injury. This model enables proper evaluation of therapeutic effects of various drugs on spinal cord injury. Through use of this model, it has been confirmed for the first time that transplantation therapy of human neural stem cells is efficacious against spinal cord injury.
摘要翻译：一种创建脊髓损伤猴模型的方法，其包括暴露猴子的颈椎硬脑膜并在硬脑膜上施加负荷; 由此造成的脊髓损伤猴模型; 以及使用该模型评价脊髓损伤治疗药物的方法。根据本发明，可以产生靠近人类的猴，因此可用作人脊髓损伤的模型。该模型能够适当评估各种药物对脊髓损伤的治疗效果。通过使用该模型，人类神经干细胞的移植治疗首次对脊髓损伤有效。

83. 发明申请

US20040268422A1 Methods for correcting mitotic spindle defects associated with somatic cell nuclear transfer in animals 审中-公开
标题翻译：纠正与动物体细胞核转移相关的有丝分裂纺锤体缺陷的方法
公开(公告)号：US20040268422A1
公开(公告)日：2004-12-30
申请号：US10821200
申请日：2004-04-09
发明人： Gerald P. Schatten , Calvin R. Simerly , Christopher S. Navara
IPC分类号： A01K067/027
CPC分类号： A01K67/00 , A01K67/027 , A01K2217/05 , A01K2227/106 , A01K2267/03 , C12N5/10 , C12N15/873 , C12N15/8776
摘要： The present invention is directed to various methodologies to make NT a practical procedure for animals, specifically, primates including human and non-human primates. Furthermore, the methods and molecular components provided by the present invention provide a practical means for producing embryos with desired characteristics. In a specific embodiment, the methodology of the present invention comprises introducing nuclei having desired characteristics along with one or more molecular components into an egg, culturing the egg to produce a viable embryo, transferring the embryo to the oviducts of a female, and producing a cloned animal.
摘要翻译：本发明涉及使NT成为动物，特别是包括人和非人灵长类动物的灵长类动物的实用程序的各种方法。此外，本发明提供的方法和分子组分提供了生产具有所需特征的胚胎的实用手段。在具体实施方案中，本发明的方法包括将具有期望特征的核以及一种或多种分子组分引入到卵中，培养卵以产生活的胚胎，将胚胎转移到雌性的输卵管中，并产生克隆动物。

84. 发明申请

US20040234948A1 Gene transfer into primate embryonic stem cells using vsv-g pseudo type simian immunodeficiency virus vectors 失效
标题翻译：使用vsv-g假型猿猴免疫缺陷病毒载体将基因转移到灵长类动物胚胎干细胞中
公开(公告)号：US20040234948A1
公开(公告)日：2004-11-25
申请号：US10479912
申请日：2004-06-22
发明人： Yutaka Hanazono , Yasuji Ueda , Yasushi Kondo , Yutaka Suzuki
IPC分类号： C12Q001/70 , C12N007/00 , C12N005/06
CPC分类号： G01N33/5073 , A01K2217/05 , A01K2227/105 , A01K2227/106 , A01K2267/025 , A01K2267/03 , A61K48/00 , C12N15/8509 , C12N15/86 , C12N2506/02 , C12N2510/00 , C12N2740/15043 , C12N2740/15045 , C12N2810/6081 , G01N33/5008 , G01N2333/22
摘要： Highly efficient gene transfer into primate-derived embryonic stem (ES) cells has successfully been achieved by using a simian immunodeficiency virus vector (SIV) pseudotyped with VSV-G protein, which is a surface glycoprotein of vesicular stomatitis virus (VSV) The present invention provides simian immunodeficiency virus vectors for gene transfer to primate ES cells. The method for gene transfer to primate ES cells using the vectors of the present invention is useful in, for example, research into embryology and disease, clinical applications, and experimental models for primates. The method is also useful in assaying and screening for genes and reagents able to enhance the specific differentiation of tissues or cells, and which are useful in preparing desired cells or tissues differentiated from ES cells.
摘要翻译：通过使用作为水泡性口炎病毒（VSV）的表面糖蛋白的VSV-G蛋白假型的猿免疫缺陷病毒载体（SIV），已经成功地实现了将高效率的基因转移到灵长类衍生的胚胎干细胞（ES）细胞中。本发明提供猿猴免疫缺陷病毒载体，用于基因转移到灵长类动物ES细胞。使用本发明的载体将基因转移到灵长类动物ES细胞的方法可用于例如胚胎学和疾病的研究，临床应用和灵长类动物的实验模型。该方法还可用于测定和筛选能够增强组织或细胞的特异性分化的基因和试剂，并且其可用于制备从ES细胞分化的所需细胞或组织。

85. 发明申请

US20040194157A1 Animal with surgically modified gastrointestinal tract and method for study of weight reduction 审中-公开
标题翻译：手术改良胃肠道动物及减肥方法
公开(公告)号：US20040194157A1
公开(公告)日：2004-09-30
申请号：US10802996
申请日：2004-03-17
发明人： Michael M. Meguid
IPC分类号： A01K067/00 , A01K067/033 , A61F005/24
CPC分类号： A01K67/027 , A01K67/0275 , A01K2217/05 , A01K2227/102 , A01K2227/105 , A01K2227/106 , A01K2227/108 , A01K2267/03
摘要： The invention comprises an animal having a presurgical substantially normal gastrointestinal tract, which gastrointestinal tract has been surgically modified such that postsurgically there is a reduction of the volume of the stomach of the gastrointestinal tract, a reduction in the digestive area of the gastrointestinal tract, a reduction in the co-mingling of food with gastric, biliary and pancreatic juices, a reduction in the presurgical gastric output of the peptide ghrelin, a reduction in the threshold for satiety, a permanent reduction in presurgical weight, and an induction of a condition of malabsorption. The surgically-altered animal may be adapted for use as an animal model in a method wherein the biological mechanisms underlying obesity and its reduction may be investigated; and, in which the molecular biological effects of surgical intervention for obesity may be investigated; and, in which the efficacy of noninvasive alternatives to surgical intervention for obesity may be investigated.
摘要翻译：本发明包括具有术前基本上正常的胃肠道的动物，其胃肠道已被外科手术改造，使得胃肠道胃的体积减少，胃肠道消化区减少，食物与胃，胆汁和胰汁的共混减少，肽生长素释放肽的胃外输出量减少，饱腹感降低，手术重量的持续降低，以及诱导的吸收不良手术改变的动物可以适用于以下方法中的动物模型：其中可以研究潜在的肥胖及其减少的生物学机制; 并且其中可以研究用于肥胖的外科手术干预的分子生物学效应; 并且可以研究非侵入性替代物对肥胖的外科手术干预的功效。

86. 发明申请

US20040157292A1 Sperm-specific cation channel, CatSper1, and uses therefor 有权
标题翻译：精子特异性阳离子通道，CatSper1，并用于此
公开(公告)号：US20040157292A1
公开(公告)日：2004-08-12
申请号：US10697863
申请日：2003-10-30
申请人： Children's Medical Center
发明人： David E. Clapham , Dejian Ren
IPC分类号： C07K014/705 , C07H021/04
CPC分类号： C07K14/705 , A01K67/0278 , A01K2217/052 , A01K2227/105 , A01K2227/106 , A01K2267/03 , G01N33/5029 , G01N33/5041 , G01N33/5044 , G01N33/68
摘要： Nucleic acid and protein sequences relating to a cation channel which is sperm-specific (CatSper1) are disclosed. The CatSper1 protein is shown to be specifically expressed in sperm and to be necessary for sperm motility. Nucleic acids, vectors, transformed cells, transgenic animals, polypeptides, and antibodies relating to the CatSper1 gene and protein are disclosed. Also provided are methods of in vitro fertilization and contraception, methods of identifying modulators of CatSper1 activity, methods of genotyping subjects with respect to CatSper1, and methods of diagnosing and treating CatSper1-mediated disorders, including infertility.
摘要翻译：公开了与精子特异性的阳离子通道相关的核酸和蛋白质序列（CatSper1）。显示CatSper1蛋白在精子中特异性表达，并且对于精子活力是必需的。公开了与CatSper1基因和蛋白相关的核酸，载体，转化细胞，转基因动物，多肽和抗体。还提供了体外受精和避孕方法，鉴定CatSper1活性的调节剂的方法，与CatSper1相关的受试者的基因分型方法，以及诊断和治疗CatSper1介导的疾病包括不孕症的方法。

87. 发明申请

US20040136960A1 Devices and processes for distribution of genetic material to mammalian limb 审中-公开
标题翻译：将遗传物质分配给哺乳动物肢体的装置和方法
公开(公告)号：US20040136960A1
公开(公告)日：2004-07-15
申请号：US10339935
申请日：2003-01-10
发明人： Jon A. Wolff , Vladimir Budker
IPC分类号： A61K048/00 , A61F005/00 , C12N015/86
CPC分类号： A61K48/0083 , A01K2227/106 , A61K48/0075
摘要： Disclosed is a process for functionally distributing genetic material to mammalian limb muscle cells comprising inserting genetic material into a limb vessel. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the genetic material with the use of an externally applied cuff to increase vascular permeability.
摘要翻译：公开了一种将遗传物质功能分布到哺乳动物肢体肌细胞的方法，包括将遗传物质插入肢体血管。通过将含有遗传物质的溶液的注入与使用外部应用的袖带组合来增加血管通透性，从而提高了输送效率和分布。

88. 发明申请

US20040031067A1 Regulation of human skin healing 审中-公开
标题翻译：调节人体皮肤愈合
公开(公告)号：US20040031067A1
公开(公告)日：2004-02-12
申请号：US10398980
申请日：2003-08-22
发明人： Meenhard Herlyn , Carola Berking , Kapaettu Satyamoorthy , Omaida Velazquez
IPC分类号： C12N005/08 , A01K067/00 , C12N015/63 , C12N015/87 , C12Q001/68
CPC分类号： C12N15/86 , A01K67/0271 , A01K2227/105 , A01K2227/106 , A61K35/28 , A61K38/1709 , A61K38/1825 , A61K38/30 , A61K38/49 , A61K48/00 , C07K14/503 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2502/28 , C12N2502/30 , C12N2710/10344 , A61K2300/00
摘要： The invention relates to new compositions and methods for preparing vascularized dermal reconstructs, vascularized skin grafts, and for enhancing vascularization in situ in response to a variety of medical conditions.
摘要翻译：本发明涉及用于制备血管化真皮重建，血管化皮肤移植物以及用于响应各种医学状况而原位增强血管化的新组合物和方法。

89. 发明授权

US5945577A Cloning using donor nuclei from proliferating somatic cells 失效
标题翻译：使用供体核从增殖体细胞克隆
公开(公告)号：US5945577A
公开(公告)日：1999-08-31
申请号：US781752
申请日：1997-01-10
申请人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
发明人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
IPC分类号： A01K67/02 , A01K67/027 , A61K35/12 , A61P1/18 , A61P3/10 , A61P9/00 , A61P9/04 , A61P13/02 , A61P17/02 , A61P21/00 , A61P25/16 , A61P25/28 , A61P31/18 , A61P35/00 , C12N5/073 , C12N5/10 , C12N15/09 , C12N15/877 , C12N5/00 , C12N15/00 , A01K67/00
CPC分类号： A01K67/0275 , A01K67/027 , C12N15/8771 , C12N15/8778 , C12N5/0603 , A01K2217/05 , A01K2227/101 , A01K2227/106 , A61K35/12 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的细胞核移植到与供体细胞相同物种的去核卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生以及用于产生包括人同种异体胚胎或干细胞在内的等基因CICM细胞的基因型和转基因基因型的倍增。通过本方法促进遗传工程或转基因哺乳动物胚胎，胎儿和后代的生产，因为供体核的分化细胞源可以被遗传修饰和克隆繁殖。

90. 发明申请

US20150201588A1 Autologous Mammalian Models Derived from Induced Pluripotent Stem Cells and Related Methods 审中-公开
标题翻译：诱导多能干细胞的自体哺乳动物模型及相关方法
公开(公告)号：US20150201588A1
公开(公告)日：2015-07-23
申请号：US14466844
申请日：2013-02-22
申请人： AMGEN INC.
发明人： Carl Alexander Kamb , Helen Y. Kim , Sungeun Kim , William Christian Fanslow, III
IPC分类号： A01K67/027 , C12Q1/68 , C12N5/071
CPC分类号： A01K67/0271 , A01K2227/106 , A01K2267/03 , A01K2267/0393 , C12N5/0679 , C12N2501/119 , C12N2501/16 , C12N2501/415 , C12N2502/13 , C12N2506/45 , C12Q1/6876
摘要： Disclosed is an autologous non-human mammalian model system derived from induced pluripotent stem (iPS) cells. Also disclosed are methods of differentiating non-human primate iPS cells, which can result in populations of cells enriched for SOX2+ or PDX1+ foregut-like cells, for CDX2+ hindgut-like cells, for CD34+ hematopoietic progenitor-like cells, or epithelial-like cells. Also disclosed is a non-human primate containing an autologous cell type of interest, which is differentiated in vitro from an induced pluripotent stem cell reprogrammed from a primary somatic cell. Methods of monitoring exogenously introduced cells within a non-human mammal are also disclosed.
摘要翻译：公开了一种衍生自诱导多能干细胞（iPS）细胞的自体非人哺乳动物模型系统。还公开了区分非人灵长类动物iPS细胞的方法，其可导致用于CDX2 +后肠样细胞，CD34 +造血祖细胞样细胞或上皮样细胞富集SOX2 +或PDX1 +前肠细胞的细胞群体。还公开了含有感兴趣的自体细胞类型的非人灵长类动物，其在体外与从原代体细胞重新编程的诱导多能干细胞分化。还公开了监测非人哺乳动物内外源细胞的方法。

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