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81. 发明申请

US20070224666A1 Alleles of the zwf gene from coryneform bacteria 有权
标题翻译：来自棒状细菌的zwf基因的等位基因
公开(公告)号：US20070224666A1
公开(公告)日：2007-09-27
申请号：US11790063
申请日：2007-04-23
申请人： Brigitte Bathe , Natalie Schischka , Georg Thierbach
发明人： Brigitte Bathe , Natalie Schischka , Georg Thierbach
IPC分类号： C12P13/04 , C12N9/04 , C07H21/04 , C12N15/74 , C12N1/21
CPC分类号： C12P13/08 , C07K14/34 , C12P13/227
摘要： The invention relates to mutants and alleles of the zwf gene of coryneform bacteria, which encode variants of the Zwf subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.
摘要翻译：本发明涉及编码葡萄糖-6-磷酸脱氢酶（EC：1.1.1.49）的Zwf亚基变体的棒状细菌的zwf基因的突变体和等位基因，以及制备氨基酸，特别是L-赖氨酸和 L-色氨酸通过使用含有所述等位基因的细菌。

82. 发明申请

US20060246554A1 Alleles of the gnd gene from coryneform bacteria 有权
标题翻译：来自棒状细菌的gnd基因的等位基因
公开(公告)号：US20060246554A1
公开(公告)日：2006-11-02
申请号：US11227138
申请日：2005-09-16
申请人： Georg Thierbach , Brigitte Bathe , Natalie Schischka
发明人： Georg Thierbach , Brigitte Bathe , Natalie Schischka
IPC分类号： C12P13/22 , C12P13/12 , C12P13/10 , C12N1/21
CPC分类号： C12P13/22 , C12N9/0006 , C12P13/08 , C12P13/227
摘要： The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.
摘要翻译：本发明涉及编码6-磷酸葡萄糖酸脱氢酶的棒状细菌的gnd基因的突变体和等位基因，其含有第329位或氨基酸序列与L-缬氨酸以外的任何氨基酸的可比较位置，以及用于生产氨基酸，优选L-赖氨酸和L-色氨酸，通过使用含有这些等位基因的细菌进行发酵。

83. 发明授权

US06921651B2 Process for the preparation of amino acids by using coryneform bacteria with attenuated 1-phosphofructokinase activity 失效
标题翻译：通过使用具有减弱的1-磷酸果糖激酶活性的棒状细菌制备氨基酸的方法
公开(公告)号：US06921651B2
公开(公告)日：2005-07-26
申请号：US10098626
申请日：2002-03-18
申请人： Mike Farwick , Brigitte Bathe , Jennifer Brehme , Klaus Huthmacher
发明人： Mike Farwick , Brigitte Bathe , Jennifer Brehme , Klaus Huthmacher
IPC分类号： C12N1/21 , C12N9/12 , C12N15/54 , C12N15/55 , C12P13/04 , C12P13/08
CPC分类号： C12N9/1205 , C12P13/04 , C12P13/08
摘要： The invention relates to a process for the preparation of L-amino acids. The process includes fermenting the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for 6-phosphofructokinase and/or the gene coding for 1-phosphofructokinase are/is attenuated, enriching the desired L-amino acid in the medium or in the cells of the bacteria, and isolating the L-amino acid. Optionally bacteria are employed in which, in addition, further genes of the biosynthetic pathway of the desired L-amino acid are enhanced, or bacteria are employed in which the metabolic pathways that diminish the formation of the desired L-amino acid are at least partly switched off.
摘要翻译：本发明涉及一种制备L-氨基酸的方法。该方法包括发酵产生所需L-氨基酸的棒状细菌，其中至少编码6-磷酸果糖激酶的基因和/或编码1-磷酸果糖激酶的基因被减毒，从而富集所需的L-氨基酸培养基或细胞中的细胞，并分离L-氨基酸。任选地，使用细菌，其中另外还需要增加所需L-氨基酸的生物合成途径的其它基因，或使用细菌，其中减少所需L-氨基酸形成的代谢途径至少部分地关闭

84. 发明申请

US20050130277A1 Nucleotide sequences coding for the pknB gene 有权
标题翻译：编码pknB基因的核苷酸序列
公开(公告)号：US20050130277A1
公开(公告)日：2005-06-16
申请号：US10940606
申请日：2004-09-15
申请人： Brigitte Bathe , Stephan Hans , Mike Farwick , Thomas Hermann
发明人： Brigitte Bathe , Stephan Hans , Mike Farwick , Thomas Hermann
IPC分类号： C12N9/12 , C12P13/04 , C07H21/04 , C12N1/21 , C12N9/10 , C12N9/42 , C12N15/74
CPC分类号： C12N9/1205 , C12P13/04 , C12Y207/01037
摘要： An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b) or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknB gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要翻译：一种分离的多核苷酸，其含有选自以下的多核苷酸序列：（a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％的多核苷酸，（b）编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，（c）与（a）或（b）的多核苷酸互补的多核苷酸和（ d）包含（a），（b）或（c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用其中至少pknB基因被扩增的棒状杆菌制备L-氨基酸的发酵方法以及使用含有本发明序列的多核苷酸作为杂交探针。

85. 发明授权

US09062332B2 Process for the fermentative preparation of sulphur-containing amino acids 有权
标题翻译：含硫氨基酸发酵制备方法
公开(公告)号：US09062332B2
公开(公告)日：2015-06-23
申请号：US13353426
申请日：2012-01-19
申请人： Frank Schneider , Stella Molck , Brigitte Bathe
发明人： Frank Schneider , Stella Molck , Brigitte Bathe
IPC分类号： C07H21/04 , C12P13/12 , C12N1/21 , C12N9/10 , C12R1/15
CPC分类号： C12P13/12 , C12N9/13 , C12R1/15 , C12Y208/01001 , Y02P20/52
摘要： Disclosed herein is a process for the fermentative preparation of sulphur-containing amino acids chosen from the group of L-methionine, L-cysteine, L-cystine, L-homocysteine and L-homocystine, comprising the steps: a) provision of a microorganism of the family Enterobacteriaceae or of a microorganism of the family Corynebacteriaceae which has an increased thiosulphate sulphurtransferase activity compared with the particular starting strain; b) fermentation of the microorganism from a) in a medium which contains an inorganic source of sulphur chosen from the group of salt of dithiosulphuric acid or a mixture of a salt of dithiosulphuric acid and a salt of sulphuric acid, a fermentation broth being obtained, and c) concentration of the sulphur-containing amino acid in the fermentation broth from b).
摘要翻译：本文公开了一种选自L-甲硫氨酸，L-半胱氨酸，L-胱氨酸，L-高半胱氨酸和L-高胱氨酸的含硫氨基酸发酵制备方法，包括以下步骤：a）提供微生物的家族肠杆菌科或科氏杆菌科的微生物，其与特定的起始菌株相比具有增加的硫代硫酸盐转移酶活性; b）从含有选自二硫代硫酸盐或二硫代硫酸盐与硫酸盐的混合物的硫的无机硫源的培养基中培养微生物，发酵培养基，和c）来自b）的发酵液中的含硫氨基酸的浓度。

86. 发明申请

US20150056666A1 VARIANTS OF THE PROMOTER OF THE GAP GENE CODING FOR GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 审中-公开
标题翻译：甘油三磷酸脱氢酶基因GAP基因编码启动子的变异
公开(公告)号：US20150056666A1
公开(公告)日：2015-02-26
申请号：US14532259
申请日：2014-11-04
申请人： Alexander RETH , Brigitte Bathe , Stephan Hans , Wilfried Claes
发明人： Alexander RETH , Brigitte Bathe , Stephan Hans , Wilfried Claes
IPC分类号： C12N15/77 , C12P13/10 , C12P13/06 , C12P13/08
CPC分类号： C12P13/08 , C12N9/0008 , C12N15/77 , C12P13/04 , C12P13/06 , C12P13/10
摘要： The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
摘要翻译：本发明涉及具有启动子活性的分离的多核苷酸，编码甘油醛-3-磷酸脱氢酶的间隙基因的启动子的变体; 以及产生和/或分泌精细化学物质的微生物，所述微生物包括具有启动子活性的分离的多核苷酸，其使各种基因与特定的起始菌株相比过表达; 以及使用微生物制备精细化学品的方法。

87. 发明授权

US08912313B2 Variants of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase 有权
标题翻译：编码甘油醛-3-磷酸脱氢酶的间隙基因的启动子的变体
公开(公告)号：US08912313B2
公开(公告)日：2014-12-16
申请号：US13534359
申请日：2012-06-27
申请人： Alexander Reth , Brigitte Bathe , Stephan Hans , Wilfried Claes
发明人： Alexander Reth , Brigitte Bathe , Stephan Hans , Wilfried Claes
IPC分类号： C12N15/11 , C12N15/77 , C12N9/02 , C12P13/08 , C12P13/10 , C12P13/04
CPC分类号： C12P13/08 , C12N9/0008 , C12N15/77 , C12P13/04 , C12P13/06 , C12P13/10
摘要： The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
摘要翻译：本发明涉及具有启动子活性的分离的多核苷酸，编码甘油醛-3-磷酸脱氢酶的间隙基因的启动子的变体; 以及产生和/或分泌精细化学物质的微生物，所述微生物包括具有启动子活性的分离的多核苷酸，其使各种基因与特定的起始菌株相比过表达; 以及使用微生物制备精细化学品的方法。

88. 发明授权

US08202706B2 Method of production of L-amino acids 有权
公开(公告)号：US08202706B2
公开(公告)日：2012-06-19
申请号：US12750968
申请日：2010-03-31
申请人： Brigitte Bathe , Wilfried Claes
发明人： Brigitte Bathe , Wilfried Claes
IPC分类号： C12P19/34 , C12N15/09 , C12N1/20 , C12N15/00 , C12N15/77
CPC分类号： C12N9/88 , C12N9/1217 , C12P13/06 , C12P13/08 , C12Y203/03001 , C12Y207/02004
摘要： An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.

89. 发明授权

US08133714B2 Process for the fermentative preparation of organic chemical compounds using coryneform bacteria in which the SugR gene is present in attenuated form 有权
标题翻译：使用其中SugR基因以减毒形式存在的棒状细菌发酵制备有机化合物的方法
公开(公告)号：US08133714B2
公开(公告)日：2012-03-13
申请号：US12232610
申请日：2008-09-19
申请人： Brigitte Bathe , Bastian Blombach , Bernhard Eikmanns , Verena Engels , Georg Thierbach , Volker Wendisch
发明人： Brigitte Bathe , Bastian Blombach , Bernhard Eikmanns , Verena Engels , Georg Thierbach , Volker Wendisch
IPC分类号： C12N1/21 , C12P13/04 , C12P13/08 , C12P13/06 , C12N15/00 , C07K14/00 , C07H21/00
CPC分类号： C12P13/08 , C12P13/06 , C12P13/10
摘要： The invention relates to a recombinant coryneform bacterium which secretes an organic chemical compound and in which the sugR gene which codes for a polypeptide having the activity of an SugR regulator has been attenuated. The invention further relates to a processes for using this bacterium for the fermentative preparation of organic chemical compounds.
摘要翻译：本发明涉及分泌有机化合物的重组棒状细菌，其中编码具有SugR调节剂活性的多肽的sugR基因已被减弱。本发明还涉及使用该细菌用于有机化合物的发酵制剂的方法。

90. 发明授权

US08119372B2 Method of production of L-amino acids 有权
标题翻译：生产L-氨基酸的方法
公开(公告)号：US08119372B2
公开(公告)日：2012-02-21
申请号：US12466466
申请日：2009-05-15
申请人： Brigitte Bathe , Wilfried Claes
发明人： Brigitte Bathe , Wilfried Claes
IPC分类号： C12P13/04 , C12P13/08 , C12P21/06 , C12M1/26 , C12N1/20
CPC分类号： C12N9/88 , C12N9/1217 , C12P13/06 , C12P13/08 , C12Y203/03001 , C12Y207/02004
摘要： An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.
摘要翻译：分离的多核苷酸编码包含SEQ ID NO：2的氨基酸序列的多肽，氨基酸序列的第5位的L-天冬氨酸被另一种蛋白原性氨基酸替代，并具有柠檬酸合酶活性。此外，载体包含多核苷酸，细菌包含载体。分离的多核苷酸包含从位置1-39包含对应于SEQ ID NO：11的位置1-39，位置40至105的核苷酸序列的核苷酸序列，编码SEQ ID NO： 12，除了L-天冬氨酸以外的各蛋白质氨基酸存在于位置5.还描述了L-氨基酸的生产方法。

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