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    • 88. 发明授权
    • Transformants producing substance pf1022 derivatives, methods for producing the same, and novel biosynthesis genes
    • 产生物质pf1022衍生物的转化体,其制备方法和新的生物合成基因
    • US07432102B2
    • 2008-10-07
    • US10472587
    • 2002-03-22
    • Koji YanaiNaomi SumidaManabu WatanabeTatsuki MoriyaTakeshi Murakami
    • Koji YanaiNaomi SumidaManabu WatanabeTatsuki MoriyaTakeshi Murakami
    • C12N1/00C12P1/00C12P17/00C12P15/00C12P13/00C12P7/00C12P7/24C12P1/02
    • C12P17/14C12N15/52
    • An objective of the present invention is to provide a method for producing substance PF1022 derivatives, in particular PF1022-220 and PF1022-260, by a direct fermentation method, and a transformant to be used for this method. According to the present invention, there is provided a transformant producing substance FF1022 derivatives, which can be obtained by introducing a genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a papA gene encoding 4-amino-4-deoxychorismate synthase. which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 2; a papB gene encoding 4-amino-4-deoxyclaismate mutase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 4; and a papC gene encoding 4-amino-4-deoxyprepbenate dehydrogenase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 6, into a phenylalanine auxotrophic host induced from an organism that produces a substance PF1022. According to the present invention, there is also provided a process of producing substance PF1022 derivative, comprising steps of culturing the above-mentioned transformant and collecting the substance PF1022 derivatives.
    • 本发明的目的是提供通过直接发酵方法生产物质PF1022衍生物,特别是PF1022-220和PF1022-260的方法,以及用于该方法的转化体。 根据本发明,提供了转化体生产物质FF1022衍生物,其可以通过将参与生物合成途径的基因从氯磺酸引入对氨基苯基丙酮酸而获得,包括编码4-氨基-4-脱氧胸苷酸的papA基因 合酶。 该基因包含编码SEQ ID NO:2的氨基酸序列的DNA序列; 编码4-氨基-4-脱氧胆酸酯变位酶的papB基因,该基因包含编码SEQ ID NO:4的氨基酸序列的DNA序列; 和编码4-氨基-4-脱氧降磷酸脱氢酶的papC基因,该基因包含编码SEQ ID NO:6的氨基酸序列的DNA序列,由产生PF1022的生物体诱导的苯丙氨酸营养缺陷型宿主中。 根据本发明,还提供了生产物质PF1022衍生物的方法,包括培养上述转化体并收集物质PF1022衍生物的步骤。