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81. 发明授权

US08859226B2 Measurement method for viable cell count, and culture medium 有权
标题翻译：活细胞计数的测定方法和培养基
公开(公告)号：US08859226B2
公开(公告)日：2014-10-14
申请号：US13390859
申请日：2011-03-25
申请人： Masamichi Muto , Fumiaki Abe , Tomoko Yaeshima
发明人： Masamichi Muto , Fumiaki Abe , Tomoko Yaeshima
IPC分类号： C12N1/20 , C12Q1/06 , C12Q1/04
CPC分类号： C12Q1/045 , C12N1/20 , C12Q1/06 , G01N2333/36
摘要： There are provided a method of identifying the longum species alone, such as Bifidobacterium longum subsp. longum alone, and measuring the viable cell count thereof, amongst the bacterial cells to be tested which include a microorganism belonging to the genus Bifidobacterium by using a culture medium that contains L-arabinose as a sole sugar source; and also a culture medium which is useful as a selective medium for the above measurement method and which is also easy to prepare.
摘要翻译：提供了一种识别长单独物种的方法，例如长双歧杆菌（Bifidobacterium longum subsp。）并且通过使用含有L-阿拉伯糖作为唯一糖源的培养基，测量其中包含属于双歧杆菌属的微生物的待测试细菌细胞中的活细胞数; 以及作为上述测定方法的选择培养基有用的培养基，也容易制备。

82. 发明授权

US08700373B2 Method for estimating survival cell count, and method for setting guaranteed cell count 有权
标题翻译：估计存活细胞计数的方法，以及设定保证细胞计数的方法
公开(公告)号：US08700373B2
公开(公告)日：2014-04-15
申请号：US12995064
申请日：2009-05-29
申请人： Fumiaki Abe , Ayako Uchijima , Ayako Horigome
发明人： Fumiaki Abe , Ayako Uchijima , Ayako Horigome
IPC分类号： G06G7/58 , G06F19/00
CPC分类号： G06F19/00 , C12Q1/06
摘要： The present invention provides a method for estimating survival cell count, which is able to calculate an accurately estimated result of the survival cell count of a specific strain in a probiotic product with respect to its storage period, and to shorten the time for developing the product, and a method for setting a guaranteed cell count, which is able to readily calculate a guaranteed cell count of a specific strain in a probiotic product within its quality guarantee period. The method for estimating the survival cell count is characterized by estimating the survival cell count nt (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log10 nt=Log10 n0−t×EXP{(AT×T+BT)w+(CT×T+DT)}, provided that t stands for the storage period (days)×1/30, nt stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (days), n0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (° C.), w stands for the water activity value of the composition, AT and CT stand for experimentally determined coefficients specific to the strain, and BT and DT stand for experimentally determined constants specific to the strain.
摘要翻译：本发明提供一种用于估计存活细胞计数的方法，其能够计算益生菌产品相对于其储存期的特定菌株的存活细胞计数的准确估计结果，并缩短开发产品的时间以及用于设定保证细胞计数的方法，其能够容易地计算其质量保证期内益生菌产品中特定菌株的保证细胞计数。用于估计存活细胞计数的方法的特征在于根据以下等式（I）估计储存后组合物中包含的特定菌株的存活细胞计数nt（CFU / g）。（I）Log10 nt = Log10 n0-t×EXP {（AT×T + BT）w +（CT×T + DT）}，条件是t代表保存期（天）×1/30，nt表示储存期t（天）后组合物中所含菌株的存活细胞计数（CFU / g），n0表示储存开始时组合物中包含的菌株的活菌数（CFU / g），T 代表储存温度（℃），w代表组合物的水活度值，AT和CT代表实验确定的菌株特异性系数，BT和DT代表对菌株特异性的实验测定常数。

83. 发明授权

US08518924B2 Agent for inhibiting visceral fat accumulation 有权
标题翻译：抑制内脏脂肪积累的药剂
公开(公告)号：US08518924B2
公开(公告)日：2013-08-27
申请号：US12841076
申请日：2010-07-21
申请人： Miyuki Tanaka , Eriko Misawa
发明人： Miyuki Tanaka , Eriko Misawa
IPC分类号： A61K31/56
CPC分类号： A61K36/896 , A23L33/105 , A23L33/30 , A61K31/575 , C07J9/00
摘要： To reduce amounts of fat accumulated in abdominal cavity and to prevent or ameliorate visceral fat type obesity, considered to be a main factor of metabolic syndrome, the present invention provides an agent or a food or drink which contains a compound having a lophenol skeleton, or an organic solvent extract or a hot water extract of a Liliaceae plant, or a fraction thereof containing the compound as an active ingredient.
摘要翻译：为了减少在腹腔中积累的脂肪的量并且被认为是代谢综合征的主要因素的内脏脂肪型肥胖症，本发明提供了含有具有薄荷骨架的化合物的药剂或食品或饮料，或百里香科植物的有机溶剂提取物或热水提取物，或其含有该化合物作为活性成分的部分。

84. 发明申请

US20130129707A1 ANTIMICROBIAL ACTIVITY ENHANCING AGENT 审中-公开
标题翻译：抗菌活性增强剂
公开(公告)号：US20130129707A1
公开(公告)日：2013-05-23
申请号：US13813240
申请日：2011-07-29
申请人： Mai Murata , Hiroyuki Wakabayashi , Koji Yamauchi
发明人： Mai Murata , Hiroyuki Wakabayashi , Koji Yamauchi
IPC分类号： A01N63/02
CPC分类号： A01N63/02 , A23K10/14 , A23K20/147 , A23K50/10 , A23K50/40 , A23K50/80 , A23L2/66 , A23L3/3526 , A23L33/19 , A23V2002/00 , A61K35/20 , A23V2200/10 , A23V2250/54248 , A23V2300/02 , A23V2300/30
摘要： An antimicrobial activity enhancing agent for lactoferrin or a lactoferrin hydrolysate, which contains a milk protein as an active ingredient and has the following properties, and a method for producing the antimicrobial activity enhancing agent, which comprises (a) the step of contacting raw milk with a cation exchange resin to adsorb proteins in the raw milk to the cation exchange resin, (b) the step of washing the cation exchange resin having undergone the adsorption treatment, and flowing an elution solvent to elute a protein fraction having an isoelectric point of 9.0 to 11.0, and (c) the step of subjecting the eluted protein fraction to membrane filtration using an ultrafiltration membrane having a molecular weight cutoff of 30 kDa to prepare the milk protein having the following properties:(A) an action of enhancing the antimicrobial activity of lactoferrin or a lactoferrin hydrolysate, (B) a molecular weight of 30 kDa or less, (C) an isoelectric point of 9.0 to 11.0.
摘要翻译：一种含有乳蛋白作为活性成分并具有以下性质的乳铁蛋白或乳铁蛋白水解产物的抗微生物活性增强剂及其制备方法，其包括：（a）将生牛奶与阳离子交换树脂，用于将原料乳中的蛋白质吸附到阳离子交换树脂中，（b）洗涤经过吸附处理的阳离子交换树脂，并使洗脱溶剂流动，洗脱等电点为9.0的蛋白质级分至11.0，和（c）使用分子量截留值为30kDa的超滤膜对洗脱的蛋白质级分进行膜过滤的步骤，以制备具有以下性质的乳蛋白：（A）增强抗微生物活性的作用的乳铁蛋白或乳铁蛋白水解物，（B）分子量为30kDa以下，（C）等电点为9.0〜11.0。

85. 发明申请

US20130068612A1 Electrolysis Water-Making Apparatus 有权
标题翻译：电解制水设备
公开(公告)号：US20130068612A1
公开(公告)日：2013-03-21
申请号：US13700219
申请日：2011-05-20
申请人： Koki Matsuyama , Tatsuya Midorikawa
发明人： Koki Matsuyama , Tatsuya Midorikawa
IPC分类号： C25B9/00 , C25B1/00
CPC分类号： C25B9/00 , C02F1/4618 , C02F1/4674 , C02F2201/004 , C02F2303/14 , C25B1/00 , C25B9/20
摘要： An electrolysis water-making apparatus (1A) includes a casing (20), an electrolytic cell (4) installed in the casing (20) and configured to perform electrolysis on a raw material solution, a raw material solution feed pump (10) configured to pump the raw material solution to the electrolytic cell (4), and a diluting unit (26) configured to dilute electrolyzed products extracted from the electrolytic cell (4) with dilution water; and provided with a bracket (30) configured to integrally attach the electrolytic cell (4) and the raw material solution feed pump (10), and detachably fixed to the inside of the casing (20) in a state in which the electrolytic cell (4) and the raw material solution feed pump (10) are attached thereto.
摘要翻译：一种电解制水装置（1A），包括壳体（20），安装在所述壳体（20）中并构造成对原料溶液进行电解的电解槽（4），配置在原料溶液供给泵将原料溶液泵送到电解槽（4），以及稀释装置（26），其用稀释水稀释从电解槽（4）中提取的电解产物; 并且具有将电解槽（4）和原料溶液供给泵（10）一体地安装在一起的托架（30），并且在电解槽（10）的状态下可拆卸地固定在壳体（20）的内部， 4）和原料溶液供给泵（10）。

86. 发明授权

US08372458B2 Continuous emulsification process for process cheese type and equipment therefor, and continuous production method for process cheese type and equipment therefor 有权
公开(公告)号：US08372458B2
公开(公告)日：2013-02-12
申请号：US12616353
申请日：2009-11-11
申请人： Hideo Shidara , Junichi Otsuji , Kiyotaka Takahashi , Takeshi Goto
发明人： Hideo Shidara , Junichi Otsuji , Kiyotaka Takahashi , Takeshi Goto
IPC分类号： A23C19/08
CPC分类号： A01J25/002 , A23C19/08 , G01N11/162
摘要： Objects of the present invention are to provide a continuous emulsification process and equipment therefor in which exact viscosity is measured inline and production conditions are controlled automatically, and a continuous production method for process cheese type and equipment therefor. The present invention provides a continuous production method for process cheese type in which process cheese type ingredients are agitated and emulsified with any agitation intensity by an agitation bar 4 while heating in the vessel which is applied with fixed back pressure, the process cheese type is flowed and held in a holding pipe 7 for a certain period and thereby the process cheese type is cooled, and then the cooled process cheese type is molded and filled and a process cheese type product 13 is produced, wherein a transducer of an oscillating viscometer is immersed in the process cheese type during holding or after cooling such that the transducer is not directly contacted with the process cheese type; and wherein agitation intensity of the agitation device and/or back pressure applied to the vessel is adjusted such that a detected value of the transducer becomes near a target value which is set in advance, and thereby production conditions are controlled automatically so that the viscosity of the process cheese type during holding or after cooling becomes near a target viscosity.

87. 发明授权

US08367608B2 Method for promoting secretion of glucagon-like peptide-1 by administering κ-casein 有权
标题翻译：通过施用胃蛋白酶来促进胰高血糖素样肽-1的分泌的方法
公开(公告)号：US08367608B2
公开(公告)日：2013-02-05
申请号：US13313724
申请日：2011-12-07
申请人： Yousuke Itoh , Kouji Nomaguchi , Muneo Yamada
发明人： Yousuke Itoh , Kouji Nomaguchi , Muneo Yamada
IPC分类号： A61K38/26 , A61P3/10
CPC分类号： A61K38/012 , A23J1/202 , A23L2/52 , A23L33/19 , A23V2002/00 , A61K38/17 , A61K38/26 , A23V2200/328 , A23V2250/54246
摘要： The invention relates to a GLP-1 secretagogue and an inhibitor of postprandial rise in blood glucose, containing κ-casein as an active ingredient, and a food or drink for promoting GLP-1 secretion and an inhibitory food or drink of postprandial rise in blood glucose, containing a milk-derived casein protein wherein κ-casein accounts for 60% by mass or more of the milk-derived casein.
摘要翻译：本发明涉及GLP-1促分泌素和餐后血糖升高抑制剂，其含有酪蛋白作为活性成分，以及用于促进GLP-1分泌的食物或饮料和餐后升高的抑制性食物或饮料含有乳衍生酪蛋白的血糖，其中，酪蛋白占乳源酪蛋白的60质量％以上。

88. 发明申请

US20120244571A1 MEASUREMENT METHOD FOR VIABLE CELL COUNT, AND CULTURE MEDIUM 审中-公开
标题翻译：可视电池计数和文化媒质的测量方法
公开(公告)号：US20120244571A1
公开(公告)日：2012-09-27
申请号：US13391855
申请日：2011-03-25
申请人： Masamichi Muto , Fumiaki Abe , Tomoko Yaeshima
发明人： Masamichi Muto , Fumiaki Abe , Tomoko Yaeshima
IPC分类号： C12Q1/06
CPC分类号： C12Q1/06 , C12N1/20 , C12Q1/045 , G01N2333/195
摘要： There are provided a method of identifying a microorganism belonging to Bifidobacterium breve alone and measuring the viable cell count thereof, amongst the bacterial cells to be tested which include a microorganism belonging to the genus Bifidobacterium by using a culture medium that contains at least one sugar alcohol selected from sorbitol and mannitol as a sole sugar source; and also a culture medium which is useful as a selective medium to be used in the above measurement method and which is also easy to prepare.
摘要翻译：提供一种通过使用含有至少一种糖醇的培养基来鉴定属于双歧杆菌的微生物在测定的细菌细胞中测定活细胞数的方法，所述细菌细胞包括双歧杆菌属的微生物选自山梨糖醇和甘露醇作为唯一的糖源; 以及作为上述测定方法中使用的也易于制备的选择性培养基的培养基。

89. 发明授权

US08263348B2 Abnormal prion protein binder, and method for detection of abnormal prion protein 失效
标题翻译：异常朊病毒蛋白结合物，异常朊病毒蛋白检测方法
公开(公告)号：US08263348B2
公开(公告)日：2012-09-11
申请号：US12742169
申请日：2008-11-20
申请人： Yoshifumi Iwamaru , Tetsuya Kuhara
发明人： Yoshifumi Iwamaru , Tetsuya Kuhara
IPC分类号： C07K14/00
CPC分类号： C07K14/79 , C07K17/08 , G01N33/6896 , G01N2333/79 , G01N2800/2828
摘要： Disclosed are: a method for detecting pathogenic isoform of prion protein as distinguished from normal prion protein in a simple manner, rapidly, with a high degree of sensitivity and quantitatively without the need of the enzymatic treatment with protease K; and a reagent for use in the method. Specifically disclosed are: a pathogenic isoform of prion protein binder which comprises lactoferrin; and a method for detecting pathogenic isoform of prion protein by using the pathogenic isoform of prion protein binder.
摘要翻译：公开了以简单的方式快速检测朊病毒蛋白的致病性同种型的方法，以高灵敏度和定量的方式检测不需要用蛋白酶K进行酶处理的方法; 和用于该方法的试剂。具体公开的是：含有乳铁蛋白的朊病毒蛋白粘合剂的致病同种型; 以及通过使用朊病毒蛋白粘合剂的致病同种型检测朊病毒蛋白的致病性同种型的方法。

90. 发明授权

US08101594B2 Method for improving pancreatic functions 有权
标题翻译：改善胰腺功能的方法
公开(公告)号：US08101594B2
公开(公告)日：2012-01-24
申请号：US11576652
申请日：2006-02-28
申请人： Miyuki Tanaka , Eriko Misawa , Noriko Habara , Muneo Yamada
发明人： Miyuki Tanaka , Eriko Misawa , Noriko Habara , Muneo Yamada
IPC分类号： A01N43/00
CPC分类号： A23L2/52 , A23L33/11 , A61K31/56 , A61K31/575 , A61K36/886 , A61K36/896
摘要： Compounds such as 4-methylcholest-7-en-3-ol, 4-methylergost-7-en-3-ol and 4-methylstigmast-7-en-3-ol which have an activity for improving pancreatic function are used as an active ingredient of a pancreas function improving drug and food or drink.
摘要翻译：使用具有改善胰腺功能的活性的化合物如4-甲基胆甾-7-烯-3-醇，4-甲基麦芽糖-7-烯-3-醇和4-甲基孕甾-7-烯-3-醇用作胰腺功能的活性成分改善药物和食物或饮料。

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