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81. 发明申请

US20120322726A1 THERAPEUTIC AGENT FOR EATING DISORDERS 审中-公开
标题翻译：用于治疗疾病的治疗剂
公开(公告)号：US20120322726A1
公开(公告)日：2012-12-20
申请号：US13580800
申请日：2011-02-23
申请人： Yuuki Somoto , Akio Yamada , Hiroshi Matsumoto
发明人： Yuuki Somoto , Akio Yamada , Hiroshi Matsumoto
IPC分类号： A61K38/01 , A61P3/04 , A61P3/00 , C12P21/06 , C07K2/00
CPC分类号： A61K38/018
摘要： Disclosed is a highly safe and effective therapeutic agent for eating disorders, which contains, as an active ingredient, a water-soluble fraction of a hydrolysis product of casein. The hydrolysis product is obtained by means of pepsin.
摘要翻译：公开了一种高度安全有效的进食障碍治疗剂，其含有酪蛋白水解产物的水溶性部分作为活性成分。水解产物通过胃蛋白酶获得。

82. 发明申请

US20120315261A1 ANTIBACTERIAL AUXILIARY AGENT COMPRISING KOMBU EXTRACT AS ACTIVE INGREDIENT, ANTIBACTERIAL COMPOSITION, AND FOOD OR BEVERAGE 审中-公开
标题翻译：含有KOMBU提取物作为活性成分，抗菌组合物和食品或饮料的抗生素辅助剂
公开(公告)号：US20120315261A1
公开(公告)日：2012-12-13
申请号：US13581037
申请日：2011-02-23
申请人： Kouichirou Shin , Koji Yamauchi
发明人： Kouichirou Shin , Koji Yamauchi
IPC分类号： A61K36/02 , A61P31/04 , A61K38/54
CPC分类号： A61K38/44 , A23L2/44 , A23L3/3472 , A23L33/105 , A61K31/727 , A61K36/02 , A61K38/443 , A61K2236/50 , C12Y101/03004 , C12Y111/01007 , A61K2300/00
摘要： Disclosed are: a method for utilizing a kombu (kelp) extract as an antibacterial auxiliary agent; and a method for utilizing a combination of the antibacterial auxiliary agent, lactoperoxidase, glucose oxidase and glucose as an antibacterial composition having a potent antibacterial activity. Specifically disclosed are: an antibacterial auxiliary agent comprising a fraction having a molecular weight of 5000 or less and produced from a kombu extract as an active ingredient; an antibacterial composition comprising the antibacterial auxiliary agent, lactoperoxidase, glucose oxidase and glucose; an antibacterial composition as mentioned above, which additionally comprises xanthane gum; and a food or beverage containing the antibacterial composition.
摘要翻译：公开了一种利用kombu（海带）提取物作为抗菌助剂的方法; 以及利用抗菌辅助剂，乳过氧化物酶，葡萄糖氧化酶和葡萄糖的组合作为具有有效抗菌活性的抗菌组合物的方法。具体公开的是：抗菌助剂，其含有分子量为5000以下的级分，由作为活性成分的棉布提取物制成; 包含抗菌助剂，乳过氧化物酶，葡萄糖氧化酶和葡萄糖的抗菌组合物; 如上所述的抗菌组合物，其还包含黄原胶; 和含有抗菌组合物的食品或饮料。

83. 发明申请

US20120309708A1 METHOD FOR TREATMENT OF LACTO-N-BIOSE-CONTAINING SOLUTION 有权
标题翻译：用于处理含有L-N-BIOSE的溶液的方法
公开(公告)号：US20120309708A1
公开(公告)日：2012-12-06
申请号：US13577167
申请日：2011-01-31
申请人： Motomitsu Kitaoka , Mamoru Nishimoto , Kanetada Shimizu
发明人： Motomitsu Kitaoka , Mamoru Nishimoto , Kanetada Shimizu
IPC分类号： A23L3/00 , C07H5/04 , A61K31/7016 , A61K8/60 , A23K1/16 , A23L1/30
CPC分类号： A23K1/1643 , A23K20/163 , A23K30/00 , A23L5/00 , A23L29/30 , A23L33/125 , A23V2002/00 , A61K9/0095 , A23V2250/28 , A23V2200/3202
摘要： The present invention relates to a method of treating a liquid composition containing lacto-N-biose, the method including: preparing a liquid composition containing lacto-N-biose having a pH at 25° C. of not less than 2.0 and not more than 5.5, and heating the liquid composition at a temperature of 65° C. or higher. The present invention can provide a treatment method that enables thermal decomposition of lacto-N-biose to be suppressed when a liquid composition containing lacto-N-biose is heated at a temperature of 65° C. or higher, a liquid preparation containing lacto-N-biose that is treated using the treatment method, a dried product produced from such a liquid preparation containing lacto-N-biose, and a method of producing a product containing lacto-N-biose.
摘要翻译：本发明涉及一种处理含有乳-N-生物的液体组合物的方法，所述方法包括：制备含有乳酸-N-生物的液体组合物，所述乳-N-生物在25℃下的pH为2.0以上且不大于 5.5，在65℃以上的温度下加热液体组合物。本发明可以提供一种处理方法，当含有乳-N-生物的液体组合物在65℃以上的温度下加热时，能够抑制乳-N-降血糖的热分解，使用处理方法处理的N-生物，由含有乳-N-生物的液体制剂制备的干燥产物，以及含有乳-N-生物的产品的制造方法。

84. 发明申请

US20120277121A1 KIT FOR DETECTION OF MICROORGANISM 审中-公开
标题翻译：用于检测微生物的工具包
公开(公告)号：US20120277121A1
公开(公告)日：2012-11-01
申请号：US13523738
申请日：2012-06-14
申请人： Shinichi Yoshida , Takashi Soejima
发明人： Shinichi Yoshida , Takashi Soejima
IPC分类号： C12Q1/68 , C40B40/06
CPC分类号： C12Q1/68 , C12Q1/689 , C12Q2523/101
摘要： A kit for use in a method for detecting live cells, injured cells and dead cells of a microorganism in a test sample by a nucleic acid amplification method is disclosed. The kit includes a cross-linker capable of cross-linking DNA by irradiation with light having a wavelength of 350 nm to 700 nm, medium, and a primer (s) for amplifying a DNA target region in the microorganism by a nucleic acid amplification method. The nucleic acid amplification method may be a PCR, LAMP, SDA, LCR or DNA microarray method. A cross-linker may be included in the kit such as ethidium monoazide, ethidium diazide, psoralen, 4,5′,8-trimethyl psoralen, or 8-methoxy psoralen.
摘要翻译：公开了一种用于通过核酸扩增方法检测测试样品中微生物的活细胞，受损细胞和死细胞的方法的试剂盒。试剂盒包括能够通过用波长为350nm至700nm的光照射DNA和通过核酸扩增方法扩增微生物中的DNA靶标区域的引物的交联剂。核酸扩增方法可以是PCR，LAMP，SDA，LCR或DNA微阵列法。试剂盒中可以包括交联剂，例如单乙叠胺，二氮嗪，补骨脂素，4,5'，8-三甲基补骨脂素或8-甲氧基补骨脂素。

85. 发明授权

US08277856B2 Method of producing acidic lactic acid bacteria beverage 有权
标题翻译：生产酸性乳酸菌饮料的方法
公开(公告)号：US08277856B2
公开(公告)日：2012-10-02
申请号：US12600096
申请日：2009-03-11
申请人： Kanetada Shimizu , Kazuya Murakami , Sachiko Takahashi , Yuta Hirano , Tatsuya Itou , Naoki Susaki
发明人： Kanetada Shimizu , Kazuya Murakami , Sachiko Takahashi , Yuta Hirano , Tatsuya Itou , Naoki Susaki
IPC分类号： A23C9/12
CPC分类号： A23C9/137 , A23C9/1234 , A23L29/244 , A23L33/135 , A23V2002/00 , A23V2250/5062
摘要： An acidic lactic acid bacteria beverage having a favorable flavor and an improved survival rate for bifidobacteria. The acidic lactic acid bacteria beverage of the present invention includes bifidobacteria and inulin, wherein the inulin is not fermented by the bifidobacteria. The inulin content is preferably within a range from 1 to 10% by mass, and the pH of the acidic lactic acid bacteria beverage is preferably within a range from 4.1 to 4.8.
摘要翻译：具有良好风味的酸性乳酸菌饮料和双歧杆菌的存活率提高。本发明的酸性乳酸菌饮料包括双歧杆菌和菊粉，其中菊粉不被双歧杆菌发酵。菊粉含量优选在1〜10质量％的范围内，酸性乳酸菌饮料的pH优选在4.1〜4.8的范围内。

86. 发明授权

US08241866B2 Method for detection of microorganism and kit for detection of microorganism 有权
标题翻译：微生物检测方法及微生物检测试剂盒
公开(公告)号：US08241866B2
公开(公告)日：2012-08-14
申请号：US12832740
申请日：2010-07-08
申请人： Shinichi Yoshida , Takashi Soejima
发明人： Shinichi Yoshida , Takashi Soejima
IPC分类号： C12Q1/02 , C12Q1/04 , C12Q1/18
CPC分类号： C12Q1/04 , C12Q1/34 , C12Q1/533 , C12Q2304/10
摘要： A kit is disclosed for preparing a measurement sample for detecting live cells, injured cells, VNC cells and dead microorganism cells in a test sample by the following steps: a) the step of treating the test sample with an enzyme having an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample, b) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison. c) the step of treating the test sample treated in the steps a) and b) with a nuclear stain agent, and d) the step of detecting the microorganism in the test sample treated with the nuclear stain agent by flow cytometry.
摘要翻译：公开了用于通过以下步骤制备用于检测测试样品中的活细胞，受损细胞，VNC细胞和死亡微生物细胞的测量样品的试剂盒：a）用具有分解细胞活性的酶处理测试样品的步骤除了微生物之外，存在于测试样品中的蛋白质或脂质的胶体颗粒，b）用拓扑异构酶毒物和/或DNA回旋酶毒物处理测试样品的步骤，c）处理测试样品的步骤在步骤a）和b）中使用核染色剂，以及d）通过流式细胞术检测用核染色剂处理的测试样品中的微生物的步骤。

87. 发明授权

US08236769B2 Agent for improving insulin resistance 有权
标题翻译：改善胰岛素抵抗的药物
公开(公告)号：US08236769B2
公开(公告)日：2012-08-07
申请号：US11916008
申请日：2006-09-22
申请人： Miyuki Tanaka , Eriko Misawa
发明人： Miyuki Tanaka , Eriko Misawa
IPC分类号： A61K31/704 , A61P3/08
CPC分类号： A61K36/896 , A23L2/52 , A23L33/105 , A61K31/704
摘要： To inhibit production of adipocytokines, in particular, adipocytokines that elicit insulin resistance and to prevent onset of pathosis caused by the insulin resistance, or improve the pathosis, the present invention provides an agent or a food or drink which contains 3-O-β-D-glucopyranosyl-4-methylergost-7-en-3-ol, or an organic solvent extract, a hot water extract or a squeezed liquid of a plant of the family Liliaceae or a fraction thereof which contains the compound as an active ingredient.
摘要翻译：为了抑制脂肪细胞因子的产生，特别是引起胰岛素抵抗和预防由胰岛素抵抗引起的病变发作或改善病变的脂肪细胞因子，本发明提供了含有3-O-和bgr的药物或食品或饮料。 D-吡喃葡萄糖基-4-甲基麦角-7-烯-3-醇或有机溶剂提取物，热水提取物或百合科植物的挤压液或其部分含有该化合物作为活性成分。

88. 发明授权

US08143018B2 Method for preparing a measurement sample for detecting live cells of a microorganism in a test sample 有权
标题翻译：制备用于检测试验样品中微生物的活细胞的测量样品的方法
公开(公告)号：US08143018B2
公开(公告)日：2012-03-27
申请号：US12832782
申请日：2010-07-08
申请人： Shinichi Yoshida , Takashi Soejima
发明人： Shinichi Yoshida , Takashi Soejima
IPC分类号： C12Q1/02 , C12Q1/04 , C12Q1/18
CPC分类号： C12Q1/04 , C12Q1/34 , C12Q1/533 , C12Q2304/10
摘要： According to the following steps, live cells, injured cells, VNC cells and dead cells of a microorganism in a test sample are detected by flow cytometry: a) the step of treating the test sample with an enzyme having an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample, b) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison. c) the step of treating the test sample treated in the steps a) and b) with a nuclear stain agent, and d) the step of detecting the microorganism in the test sample treated with the nuclear stain agent by flow cytometry.
摘要翻译：根据以下步骤，通过流式细胞术检测试验样品中微生物的活细胞，受损细胞，VNC细胞和死细胞：a）用具有分解细胞以外的细胞活性的酶处理测试样品的步骤微生物的那些，存在于测试样品中的蛋白质或脂质的胶体颗粒，b）用拓扑异构酶毒物和/或DNA回旋酶毒物处理测试样品的步骤，c）处理在测试样品中处理的测试样品的步骤步骤a）和b），以及d）通过流式细胞术检测用核染色剂处理的测试样品中的微生物的步骤。

89. 发明申请

US20110112813A1 METHOD FOR ESTIMATING SURVIVAL CELL COUNT, AND METHOD FOR SETTING GUARANTEED CELL COUNT 有权
标题翻译：评估存活细胞计数的方法和设定保证细胞计数的方法
公开(公告)号：US20110112813A1
公开(公告)日：2011-05-12
申请号：US12995064
申请日：2009-05-29
申请人： Fumiaki Abe , Ayako Uchijima , Ayako Horigome
发明人： Fumiaki Abe , Ayako Uchijima , Ayako Horigome
IPC分类号： G06F17/11
CPC分类号： G06F19/00 , C12Q1/06
摘要： The present invention provides a method for estimating survival cell count, which is able to calculate an accurately estimated result of the survival cell count of a specific strain in a probiotic product with respect to its storage period, and to shorten the time for developing the product, and a method for setting a guaranteed cell count, which is able to readily calculate a guaranteed cell count of a specific strain in a probiotic product within its quality guarantee period. The method for estimating the survival cell count is characterized by estimating the survival cell count nt (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log10 nt=Log10 n0−t×EXP{(AT×T+BT)w+(CT×T+DT)}, provided that t stands for the storage period (days)×1/30, nt stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (days), n0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (° C.), w stands for the water activity value of the composition, AT and CT stand for experimentally determined coefficients specific to the strain, and BT and DT stand for experimentally determined constants specific to the strain.
摘要翻译：本发明提供一种用于估计存活细胞计数的方法，其能够计算益生菌产品相对于其储存期的特定菌株的存活细胞计数的准确估计结果，并缩短开发产品的时间以及用于设定保证细胞计数的方法，其能够容易地计算其质量保证期内益生菌产品中特定菌株的保证细胞计数。用于估计存活细胞计数的方法的特征在于根据以下等式（I）估计储存后组合物中包含的特定菌株的存活细胞计数nt（CFU / g）。（I）Log10 nt = Log10 n0-t×EXP {（AT×T + BT）w +（CT×T + DT）}，条件是t代表保存期（天）×1/30，nt表示储存期t（天）后组合物中所含的菌株的存活细胞计数（CFU / g），n0表示储存开始时组合物中包含的菌株的活菌数（CFU / g），T 代表储存温度（℃），w代表组合物的水活度值，AT和CT代表实验确定的菌株特异性系数，BT和DT代表对菌株特异性的实验测定常数。

90. 发明授权

US07932069B2 Process for producing lactoperoxidase 有权
标题翻译：生产过氧化物酶的方法
公开(公告)号：US07932069B2
公开(公告)日：2011-04-26
申请号：US10597347
申请日：2005-02-08
申请人： Nobuo Ichihashi , Koji Yamauchi , Kouichirou Shin , Tetsuya Ando
发明人： Nobuo Ichihashi , Koji Yamauchi , Kouichirou Shin , Tetsuya Ando
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , B01D61/145 , B01D61/16 , B01D2311/04 , B01J39/07 , B01J49/53 , B01D2311/12 , B01D2311/2623
摘要： A process for producing lactoperoxidase, including: a step (1) for bringing one or more milk materials into contact with a cation exchanger having weakly acidic groups as ion exchange groups to thereby effect adsorption treatment; a step (2) for washing the cation exchanger after the adsorption treatment; a step (3) for bringing the cation exchanger after the washing into contact with a leaching solvent to thereby obtain a leaching solution having lactoperoxidase eluted into the leaching solvent; a step (4) for concentrating the leaching solution through an ultrafiltration membrane to thereby effect precipitation in the concentrated leaching solution; and a step (5) for obtaining a lactoperoxidase solution by removing the precipitation from the concentrated leaching solution.
摘要翻译：一种生产乳过氧化物酶的方法，包括：使一种或多种乳物质与具有弱酸性基团的阳离子交换剂作为离子交换基团接触从而进行吸附处理的步骤（1）用于在吸附处理后洗涤阳离子交换剂的步骤（2）; 使洗涤后的阳离子交换剂与浸出溶剂接触的工序（3），得到将乳过氧化物酶洗脱到浸出溶剂中的浸出液; 用于通过超滤膜浓缩浸出溶液从而在浓缩浸出溶液中进行沉淀的步骤（4）; 以及通过从浓缩浸出溶液中除去沉淀来获得乳过氧化物酶溶液的步骤（5）。

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