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71. 发明申请

US20020116735A1 Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis 审中-公开
标题翻译：编码涉及非常长链脂肪酸合成的植物酶的核酸
公开(公告)号：US20020116735A1
公开(公告)日：2002-08-22
申请号：US09892325
申请日：2001-06-26
申请人： The University of British Columbia
发明人： Ljerka Kunst , Anthony A. Millar
IPC分类号： C12N015/82 , A01H005/00 , C12N009/10 , C12N015/54 , C12P007/64 , C12N015/29 , C12N005/04
CPC分类号： C12N15/8222 , C12N9/1029 , C12N15/8223 , C12N15/8247 , C12N15/8289
摘要： Nucleic acid molecules encoding an enzyme involved in very long chain fatty acid (VLCFA) elongation in plants are disclosed. The invention includes a cDNA, genomic clone and encoded protein, as well as plants having modified VLCFA composition, such as modified epicuticular waxes, and methods of making such plants.
摘要翻译：公开了编码参与植物中非常长链脂肪酸（VLCFA）伸长的酶的核酸分子。本发明包括cDNA，基因组克隆和编码的蛋白质，以及具有改良的VLCFA组合物的植物，例如经修饰的上皮蜡，以及制备这种植物的方法。

72. 发明授权

US06410826B1 Reduction of lignin biosynthesis in transgenic plants 失效
标题翻译：在转基因植物中减少木质素生物合成
公开(公告)号：US06410826B1
公开(公告)日：2002-06-25
申请号：US09339998
申请日：1999-06-25
申请人： Martin F. Yanofsky , Sarah Liljegren , Cristina Ferrandiz
发明人： Martin F. Yanofsky , Sarah Liljegren , Cristina Ferrandiz
IPC分类号： C12N1582
CPC分类号： C12N15/8222 , C12N15/8223 , C12N15/8255
摘要： The present invention provides methods of selectively controlling lignin biosynthesis in plants such that lignification is reduced or enhanced, as desired. The invention provides, for example, a method of reducing lignification in a vascular plant by ectopically expressing a nucleic acid molecule encoding an AGL8-like gene product in the plant, whereby lignification is reduced due to ectopic expression of the nucleic acid molecule. An AGL8-like gene product useful in the invention can have, for example, substantially the amino acid sequence of an AGL8 ortholog such as Arabidopsis AGL8 (SEQ ID NO:2).
摘要翻译：本发明提供了选择性地控制植物中木质素生物合成的方法，以便根据需要减少或增强木质素化。本发明提供了例如通过在植物中异源表达编码AGL8样基因产物的核酸分子来降低维管植物中的木质素化的方法，由此由于核酸分子的异位表达而使木质素被还原。可用于本发明的AGL8样基因产物可以具有例如基本上AGL8直系同源物如拟南芥AGL8（SEQ ID NO：2）的氨基酸序列。

73. 发明授权

US06362398B1 ClpP plastid promoter sequence 有权
标题翻译： ClpP质体启动子序列
公开(公告)号：US06362398B1
公开(公告)日：2002-03-26
申请号：US09265919
申请日：1999-03-10
申请人： Peter Bernard Heifetz
发明人： Peter Bernard Heifetz
IPC分类号： A01H500
CPC分类号： C12N15/8214 , C12N15/8223
摘要： A novel promoter isolated from the 5′ flanking region upstream of the coding sequence of the Arabidopsis plastid clpP gene is described. Also described are a novel method for utilizing protein-coding regions of plastid genes to isolate intervening regulatory sequences and a novel method for improving plastid transformation efficiency using exogenous plastid promoters that differ in nucleotide sequence from native plastid promoters.
摘要翻译：描述了从拟南芥质体clpP基因的编码序列上游的5'侧翼区分离的新型启动子。还描述了一种利用质体基因的蛋白质编码区域来分离干扰调控序列的新方法，以及使用与天然质体启动子不同的核苷酸序列的外源质体启动子来提高质体转化效率的新方法。

74. 发明申请

US20010016956A1 Herbicide-tolerant protox genes produced by DNA shuffling 失效
标题翻译：通过DNA洗牌产生的除草剂耐药性protox基因
公开(公告)号：US20010016956A1
公开(公告)日：2001-08-23
申请号：US09730525
申请日：2000-12-05
发明人： Eric R. Ward , Sandra L. Volrath , Marie A. Johnson , Sharon L. Potter
IPC分类号： C12N015/82
CPC分类号： C12Y103/03004 , C12N5/04 , C12N9/001 , C12N15/8214 , C12N15/8216 , C12N15/8223 , C12N15/8274
摘要： The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant . Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme. The present invention further provides shuffled DNA molecules encoding protox enzymes having enhanced tolerance to a herbicide that inhibits the protox activity encoded by a template DNA molecule from which the shuffled DNA molecule is derived.
摘要翻译：本发明提供了编码大豆，小麦，棉花，甜菜，油菜，稻，高粱和甘蔗的原卟啉原氧化酶（protox）酶的新型DNA序列。此外，本发明教导了耐除草剂的脱毒酶的修饰形式。还提供了表达本文教导的除草剂耐受性前体毒素酶的植物。这些植物可以通过将天然protox基因突变为抗性形式而被设计用于抵抗前毒素抑制剂，或者它们可以用编码除草剂耐受形式的植物泌毒素酶的基因转化。本发明进一步提供编码具有增强的对除草剂的耐受性的排毒酶的洗牌DNA分子，所述除草剂抑制由洗牌的DNA分子衍生的模板DNA分子编码的前导毒素活性。

75. 发明授权

US5866421A Enhanced expression in a plant plastid 失效
标题翻译：在植物质体中增强表达
公开(公告)号：US5866421A
公开(公告)日：1999-02-02
申请号：US593205
申请日：1996-01-29
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
IPC分类号： A01H5/00 , C07K14/325 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/02 , C12N5/14
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/8214 , C12N15/8216 , C12N15/8223
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide increased expression in the plastids of a plant or plant cell of a protein which produces a phenotype which is present when the plant or plant cell is grown in the absence of means for selecting transformed cells. Expression of the Bacillus thuringiensis bacterial protoxin in a plant chloroplast is exemplified.
摘要翻译：用于植物细胞遗传工程的新型组合物和方法可用于提供蛋白质的植物或植物细胞的质体中增加的表达，该蛋白质产生当在不存在选择转化的方法的情况下生长植物或植物细胞时存在的表型细胞。苏云金芽孢杆菌细菌原毒素在植物叶绿体中的表达。

76. 发明授权

US5583036A Regeneration of cotton plant in suspension culture 失效
标题翻译：棉花植物在悬浮培养中的再生
公开(公告)号：US5583036A
公开(公告)日：1996-12-10
申请号：US486380
申请日：1995-06-07
申请人： Thirumale S. Rangan , Kanniah Rajasekaran
发明人： Thirumale S. Rangan , Kanniah Rajasekaran
IPC分类号： A01H1/02 , A01H4/00 , A01H5/10 , C07K14/325 , C12N5/04 , C12N15/54 , C12N15/82 , C12N15/84 , C12N5/00
CPC分类号： A01H5/10 , A01H1/02 , A01H4/001 , A01H4/005 , A01H4/008 , C07K14/325 , C12N15/8205 , C12N15/8222 , C12N15/8223 , C12N15/8275 , C12N15/8286 , C12N5/04
摘要： A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.
摘要翻译：一种从体细胞再生棉花植物的方法。该方法包括提供棉花外植体，在补充有葡萄糖作为主要碳源的愈伤组织生长培养基中培养外植体，直到酚类化合物的分泌停止，并从外植体形成未分化的愈伤组织，并在补充的愈伤组织生长培养基中培养未分化的愈伤组织以蔗糖为主要碳源，直到从愈伤组织形成胚性愈伤组织为止。

77. 发明授权

US5545817A Enhanced expression in a plant plastid 失效
公开(公告)号：US5545817A
公开(公告)日：1996-08-13
申请号：US209649
申请日：1994-03-11
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
IPC分类号： A01H5/00 , C07K14/325 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/02 , A01H4/00 , C12N5/04
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/8214 , C12N15/8216 , C12N15/8223
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide increased expression in the plastids of a plant or plant cell of a protein which produces a phenotype which is present when the plant or plant cell is grown in the absence of means for selecting transformed cells. Expression of the Bacillus thuringiensis bacterial protoxin in a plant chloroplast is exemplified.

78. 发明授权

US5244802A Regeneration of cotton 失效
标题翻译：棉花再生
公开(公告)号：US5244802A
公开(公告)日：1993-09-14
申请号：US680048
申请日：1991-03-29
申请人： Thirumale S. Rangan
发明人： Thirumale S. Rangan
IPC分类号： A01H1/02 , A01H4/00 , A01H5/10 , C07K14/325 , C12N5/04 , C12N15/54 , C12N15/82 , C12N15/84
CPC分类号： A01H5/10 , A01H1/02 , A01H4/001 , A01H4/005 , A01H4/008 , C07K14/325 , C12N15/8205 , C12N15/8222 , C12N15/8223 , C12N15/8275 , C12N15/8286 , C12N5/04
摘要： There are provided methods for regenerating cotton by tissue and suspension culture starting with explants which are the hypocotyl, cotyledon or immature embryos. This also taught methods to transform cotton and improve cotton by selective growth.
摘要翻译：提供了通过组织和悬浮培养物再生棉花的方法，从作为下胚轴，子叶或未成熟胚的外植体开始。这也教导了通过选择性生长改造棉花和改善棉花的方法。

79. 发明申请

US20190203214A1 PROTEIN PRODUCTION IN PLANT CELLS 审中-公开
公开(公告)号：US20190203214A1
公开(公告)日：2019-07-04
申请号：US16311806
申请日：2017-06-20
申请人： Algentech SAS
发明人： Alexander SOROKIN , Isabelle MALCUIT , Anna JAKUBIEC , Thibaud CAYLA
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8214 , C12N15/8223 , C12N15/8257 , C12N15/8289
摘要： Improved methods of producing nucleic acid molecules, proteins and peptides in host cells and genetically engineered plants, vectors and constructs therefor.

80. 发明申请

US20190127749A1 RESOURCE USE EFFICIENCY IMPROVEMENT IN PLANTS 审中-公开
公开(公告)号：US20190127749A1
公开(公告)日：2019-05-02
申请号：US15929041
申请日：2018-09-17
申请人： Koch Biological Solutions, LLC
发明人： Graham J. Hymus , T. Lynne Reuber , Colleen M. Marion , Oliver J. Ratcliffe , Jeffrey M. Libby
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8223 , Y02A40/146
摘要： Polynucleotides and polypeptides incorporated into expression vectors are introduced into plants and were ectopically expressed. These polypeptides may confer at least one regulatory activity and increased yield, increased resource use efficiency, increased water use efficiency, increased light use efficiency, increased photosynthetic capacity, increased photosynthetic rate, increased photosynthetic resource use efficiency, greater vigor, and/or greater biomass as compared to a control plant.

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