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71. 发明申请

US20030131385A1 Polynucleotide sequences from rice 失效
标题翻译：水稻多核苷酸序列
公开(公告)号：US20030131385A1
公开(公告)日：2003-07-10
申请号：US10356820
申请日：2003-03-17
发明人： J. Ranjan Perera , Min Lu , Animesh Ray
IPC分类号： A01H001/00 , C07H021/04 , C12N009/00 , C12N015/82 , C12P021/02 , C12N005/04
CPC分类号： C12N15/8216 , C12N15/8222 , C12N15/8223 , C12N15/8227 , C12N15/8231
摘要： The present invention provides polynucleotides isolated from eucaryotic organisms which are structural genes or promoters. Such isolated polynucleotides are particularly useful in the modification of gene expression in plants. This invention also relates to compositions isolated from plants and their use in the modification of gene activation and/or expression. In a specific embodiment, the subject invention provides plant polynucleotide sequences encoding promoters that are components of the cellular activation and transcription apparatus and the use of such polynucleotide sequences in the modification of expression of genes.
摘要翻译：本发明提供从作为结构基因或启动子的真核生物分离的多核苷酸。这种分离的多核苷酸特别可用于植物中基因表达的修饰。本发明还涉及从植物中分离的组合物及其在基因激活和/或表达修饰中的用途。在具体实施方案中，本发明提供了编码作为细胞活化和转录装置的组分的启动子的植物多核苷酸序列以及这些多核苷酸序列在修饰基因表达中的用途。

72. 发明授权

US06587805B2 Testing a write transducer as a reader 失效
标题翻译：测试写入传感器作为阅读器
公开(公告)号：US06587805B2
公开(公告)日：2003-07-01
申请号：US09790846
申请日：2001-02-20
申请人： Mohammad Shoaib Aslami
发明人： Mohammad Shoaib Aslami
IPC分类号： G01L2500
CPC分类号： C12N9/88 , C12N15/8216 , C12N15/8223 , C12N15/8237 , G11B5/455 , G11B5/4806 , G11B2005/0013 , G11B2005/0016
摘要： A system for testing inductive write head elements of a head stack assembly (“HSA”). A completed HSA is placed in a test apparatus. Test probes are aligned and placed in electrical contact with the head elements, preferably at a location between the head and the preamplifier. The HSA is then placed in a changing magnetic field. A low frequency magnetic field may be applied to test the head elements. The changing magnetic field induces electrical signals within the head elements. By analyzing these electrical signals, the condition and characteristics of the each head element can be determined.
摘要翻译：一种用于测试磁头堆叠组件（“HSA”）的感应写头元件的系统。将完成的HSA放置在测试装置中。测试探针对准并放置成与头部元件电接触，优选地位于头部和前置放大器之间的位置。然后将HSA置于变化的磁场中。可以应用低频磁场来测试头元件。磁场变化引起磁头元件内的电信号。通过分析这些电信号，可以确定每个头元件的状态和特性。

73. 发明申请

US20030106097A1 Chitinase encoding DNA molecule from cotton expressed preferentially in fibers during secondary cell wall deposition and the corresponding promoter 审中-公开
标题翻译：来自棉花的几丁质酶编码DNA分子优先在二次细胞壁沉积期间在纤维中表达，并且相应的启动子
公开(公告)号：US20030106097A1
公开(公告)日：2003-06-05
申请号：US09918083
申请日：2001-07-30
发明人： Candace H. Haigler , Hong Zhang , Chunfa Wu , Chun-Hua Wan
IPC分类号： A01H005/00 , C07H021/04 , C12N009/24 , C12N005/04 , C12P021/02
CPC分类号： C12N15/8234 , C12N9/2442 , C12N15/8223 , C12N15/8261 , C12Y302/01014 , Y02A40/146
摘要： The present invention relates to an isolated nucleic acid molecule encoding an endogenous cotton chitinase and its promoter, which are preferentially expressed in fibers during secondary wall deposition. The polypeptide encoded by the nucleic acid molecule, a DNA construct linking the isolated nucleic acid molecule with a promoter, the DNA construct incorporated in an expression system, a host cell, a plant, or a plant seed are also disclosed. The present invention also relates to a DNA construct linking the isolated promoter with a second DNA as well as expression systems, host cells, plants, or plant seeds containing the DNA construct. Methods of imparting resistance to insects and fungi, regulating the fiber cellulose content, and methods of expressing a gene preferentially in fibers during secondary wall deposition are also disclosed.
摘要翻译：本发明涉及编码内源性棉花几丁质酶及其启动子的分离的核酸分子，其在次级壁沉积期间优先在纤维中表达。还公开了由核酸分子编码的多肽，将分离的核酸分子与启动子连接的DNA构建体，并入表达系统中的DNA构建体，宿主细胞，植物或植物种子。本发明还涉及将分离的启动子与第二DNA以及含有DNA构建体的表达系统，宿主细胞，植物或植物种子连接的DNA构建体。还公开了赋予昆虫和真菌抗性的方法，调节纤维素含量，以及在次级壁沉积期间优先在纤维中表达基因的方法。

74. 发明申请

US20030101474A1 DNA fragment having promoter function 失效
标题翻译：具有启动子功能的DNA片段
公开(公告)号：US20030101474A1
公开(公告)日：2003-05-29
申请号：US09802937
申请日：2001-03-12
申请人： Nissan Chemical Industries, Ltd.
发明人： Hirofumi Uchimiya , Satoshi Arai , Takaomi Fushimi , Michito Tagawa , Hiromitsu Fukuzawa
IPC分类号： A01H005/00 , C12N001/21 , C12N015/82
CPC分类号： C12N9/1229 , C12N15/8216 , C12N15/8223 , C12N15/8237 , C12N15/8238
摘要： DNA derived from a gene encoding rice adenylate kinase which has a promoter function in a plant. A vector containing the DNA having a promoter function. A bacterium containing the vector, a plant cell transformed with the vector, and a plant regenerated from the plant cell and its seed. The DNA having a promoter function can be ligated with a structural gene such as a reporter gene and integrated into a vector such as an expression vector. When the DNA having a promoter function is ligated to a vector together with a structural gene encoding a protein and transformed into a host cell, the structural gene is expressed. Further, the expression of a desired gene can be regulated by ligating the desired gene under the control of the DNA fragment.
摘要翻译：衍生自在植物中具有启动子功能的编码水稻腺苷酸激酶的基因的DNA。含有启动子功能的DNA的载体。含有载体的细菌，用载体转化的植物细胞和从植物细胞及其种子再生的植物。具有启动子功能的DNA可与结构基因如报告基因连接，并整合到载体如表达载体中。当具有启动子功能的DNA与编码蛋白质的结构基因一起连接到载体并转化到宿主细胞中时，表达结构基因。此外，可以通过在DNA片段的控制下连接所需基因来调节所需基因的表达。

75. 发明申请

US20020173017A1 Wooden leg gene, promoter and uses thereof 失效
标题翻译：木腿基因，启动子及其用途
公开(公告)号：US20020173017A1
公开(公告)日：2002-11-21
申请号：US10135322
申请日：2002-04-30
发明人： Philip N. Benfey , Yrjo Helariutta , Ari Pekka Mahonen , Albertus Wilhelm Martinus Bonke , Leila Kauppinen , Marjukka Riikonen
IPC分类号： C12N009/00 , C07H021/04 , C12P021/02 , C12N005/04
CPC分类号： C12N15/8223 , C07K14/415 , C12N15/8227 , C12N15/8234 , C12N15/8261 , Y02A40/146
摘要： The structure and function of a regulatory gene, WOODEN LEG (WOL), is described. The WOL gene is expressed specifically in the vasculature during early stages of embryogenesis with expression continuing throughout development. WOL encodes a novel two component signal transducer and is required for asymmetric cell divisions during vascular tissue morphogenesis. Also described are WOL nucleic acids, WOL gene products, (including, but not limited to, transcriptional products such as mRNAs, antisense, and ribozyme molecules, and translational products such WOL proteins, polypeptides, peptides and fusion proteins related thereto), antibodies to WOL gene products, WOL promoters and regulatory regions and the use of the foregoing to improve agronomically valuable plants.
摘要翻译：描述了调节基因WOODEN LEG（WOL）的结构和功能。 WOL基因在胚胎发生的早期阶段在脉管系统中表达，其表达在整个发育过程中持续表达。 WOL编码一种新型的双组分信号传感器，并且在血管组织形态发生期间需要不对称细胞分裂。还描述了WOL核酸，WOL基因产物（包括但不限于转录产物，如mRNA，反义和核酶分子，以及与其相关的转录产物如WOL蛋白，多肽，肽和融合蛋白），抗体 WOL基因产物，WOL启动子和调控区以及上述用途来改善农艺有价值的植物。

76. 发明申请

US20020116735A1 Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis 审中-公开
标题翻译：编码涉及非常长链脂肪酸合成的植物酶的核酸
公开(公告)号：US20020116735A1
公开(公告)日：2002-08-22
申请号：US09892325
申请日：2001-06-26
申请人： The University of British Columbia
发明人： Ljerka Kunst , Anthony A. Millar
IPC分类号： C12N015/82 , A01H005/00 , C12N009/10 , C12N015/54 , C12P007/64 , C12N015/29 , C12N005/04
CPC分类号： C12N15/8222 , C12N9/1029 , C12N15/8223 , C12N15/8247 , C12N15/8289
摘要： Nucleic acid molecules encoding an enzyme involved in very long chain fatty acid (VLCFA) elongation in plants are disclosed. The invention includes a cDNA, genomic clone and encoded protein, as well as plants having modified VLCFA composition, such as modified epicuticular waxes, and methods of making such plants.
摘要翻译：公开了编码参与植物中非常长链脂肪酸（VLCFA）伸长的酶的核酸分子。本发明包括cDNA，基因组克隆和编码的蛋白质，以及具有改良的VLCFA组合物的植物，例如经修饰的上皮蜡，以及制备这种植物的方法。

77. 发明授权

US06410826B1 Reduction of lignin biosynthesis in transgenic plants 失效
标题翻译：在转基因植物中减少木质素生物合成
公开(公告)号：US06410826B1
公开(公告)日：2002-06-25
申请号：US09339998
申请日：1999-06-25
申请人： Martin F. Yanofsky , Sarah Liljegren , Cristina Ferrandiz
发明人： Martin F. Yanofsky , Sarah Liljegren , Cristina Ferrandiz
IPC分类号： C12N1582
CPC分类号： C12N15/8222 , C12N15/8223 , C12N15/8255
摘要： The present invention provides methods of selectively controlling lignin biosynthesis in plants such that lignification is reduced or enhanced, as desired. The invention provides, for example, a method of reducing lignification in a vascular plant by ectopically expressing a nucleic acid molecule encoding an AGL8-like gene product in the plant, whereby lignification is reduced due to ectopic expression of the nucleic acid molecule. An AGL8-like gene product useful in the invention can have, for example, substantially the amino acid sequence of an AGL8 ortholog such as Arabidopsis AGL8 (SEQ ID NO:2).
摘要翻译：本发明提供了选择性地控制植物中木质素生物合成的方法，以便根据需要减少或增强木质素化。本发明提供了例如通过在植物中异源表达编码AGL8样基因产物的核酸分子来降低维管植物中的木质素化的方法，由此由于核酸分子的异位表达而使木质素被还原。可用于本发明的AGL8样基因产物可以具有例如基本上AGL8直系同源物如拟南芥AGL8（SEQ ID NO：2）的氨基酸序列。

78. 发明授权

US06362398B1 ClpP plastid promoter sequence 有权
标题翻译： ClpP质体启动子序列
公开(公告)号：US06362398B1
公开(公告)日：2002-03-26
申请号：US09265919
申请日：1999-03-10
申请人： Peter Bernard Heifetz
发明人： Peter Bernard Heifetz
IPC分类号： A01H500
CPC分类号： C12N15/8214 , C12N15/8223
摘要： A novel promoter isolated from the 5′ flanking region upstream of the coding sequence of the Arabidopsis plastid clpP gene is described. Also described are a novel method for utilizing protein-coding regions of plastid genes to isolate intervening regulatory sequences and a novel method for improving plastid transformation efficiency using exogenous plastid promoters that differ in nucleotide sequence from native plastid promoters.
摘要翻译：描述了从拟南芥质体clpP基因的编码序列上游的5'侧翼区分离的新型启动子。还描述了一种利用质体基因的蛋白质编码区域来分离干扰调控序列的新方法，以及使用与天然质体启动子不同的核苷酸序列的外源质体启动子来提高质体转化效率的新方法。

79. 发明申请

US20010016956A1 Herbicide-tolerant protox genes produced by DNA shuffling 失效
标题翻译：通过DNA洗牌产生的除草剂耐药性protox基因
公开(公告)号：US20010016956A1
公开(公告)日：2001-08-23
申请号：US09730525
申请日：2000-12-05
发明人： Eric R. Ward , Sandra L. Volrath , Marie A. Johnson , Sharon L. Potter
IPC分类号： C12N015/82
CPC分类号： C12Y103/03004 , C12N5/04 , C12N9/001 , C12N15/8214 , C12N15/8216 , C12N15/8223 , C12N15/8274
摘要： The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant . Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme. The present invention further provides shuffled DNA molecules encoding protox enzymes having enhanced tolerance to a herbicide that inhibits the protox activity encoded by a template DNA molecule from which the shuffled DNA molecule is derived.
摘要翻译：本发明提供了编码大豆，小麦，棉花，甜菜，油菜，稻，高粱和甘蔗的原卟啉原氧化酶（protox）酶的新型DNA序列。此外，本发明教导了耐除草剂的脱毒酶的修饰形式。还提供了表达本文教导的除草剂耐受性前体毒素酶的植物。这些植物可以通过将天然protox基因突变为抗性形式而被设计用于抵抗前毒素抑制剂，或者它们可以用编码除草剂耐受形式的植物泌毒素酶的基因转化。本发明进一步提供编码具有增强的对除草剂的耐受性的排毒酶的洗牌DNA分子，所述除草剂抑制由洗牌的DNA分子衍生的模板DNA分子编码的前导毒素活性。

80. 发明授权

US5866421A Enhanced expression in a plant plastid 失效
标题翻译：在植物质体中增强表达
公开(公告)号：US5866421A
公开(公告)日：1999-02-02
申请号：US593205
申请日：1996-01-29
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
IPC分类号： A01H5/00 , C07K14/325 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/02 , C12N5/14
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/8214 , C12N15/8216 , C12N15/8223
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide increased expression in the plastids of a plant or plant cell of a protein which produces a phenotype which is present when the plant or plant cell is grown in the absence of means for selecting transformed cells. Expression of the Bacillus thuringiensis bacterial protoxin in a plant chloroplast is exemplified.
摘要翻译：用于植物细胞遗传工程的新型组合物和方法可用于提供蛋白质的植物或植物细胞的质体中增加的表达，该蛋白质产生当在不存在选择转化的方法的情况下生长植物或植物细胞时存在的表型细胞。苏云金芽孢杆菌细菌原毒素在植物叶绿体中的表达。

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