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    • 72. 发明授权
    • Editing control for spatial deformations
    • 编辑空间变形控制
    • US07812850B1
    • 2010-10-12
    • US11771726
    • 2007-06-29
    • John Nelson
    • John Nelson
    • G09G5/00G06T17/00G06T15/30
    • G06T19/20G06T11/60G06T2219/2021
    • Systems, methods, and computer readable media for editing controls for spatial deformations are described. One embodiment includes a method including the steps of causing an image having at least one unmodified object to be displayed in a window, receiving a deformation of the unmodified object, generating a deformed object based at least in part on the deformation and the unmodified object, and causing the deformed object to be displayed in the window. The method further includes the steps of causing a representation of the unmodified object to be displayed in the window with the deformed object, receiving a selection of a point within the representation of the unmodified object, receiving a modification of a first parameter associated with the unmodified object at the point, regenerating the deformed object based at least in part on the deformation, the unmodified object, and the modified first parameter, and causing the regenerated deformed object to be displayed in the window.
    • 描述了用于编辑用于空间变形的控制的系统,方法和计算机可读介质。 一个实施例包括一种方法,包括以下步骤:使具有至少一个未修改对象的图像显示在窗口中,接收未修改对象的变形,至少部分地基于变形和未修改对象产生变形对象, 并使变形对象显示在窗口中。 该方法还包括以下步骤:使未修改对象的表示与变形对象一起显示在窗口中,接收对未修改对象的表示内的点的选择,接收与未修改的对象相关联的第一参数的修改 至少部分地基于变形,未修改对象和修改的第一参数再生变形对象,并且使再生的变形对象在窗口中显示。
    • 73. 发明申请
    • STEREOSCOPIC 3D LIQUID CRYSTAL DISPLAY APPARATUS WITH STRUCTURED LIGHT GUIDE SURFACE
    • 立体三维液晶显示装置结构光导表面
    • US20080084518A1
    • 2008-04-10
    • US11865916
    • 2007-10-02
    • Robert BrottJohn SchultzJohn Nelson
    • Robert BrottJohn SchultzJohn Nelson
    • G02F1/13357
    • G02B27/2264G02B6/0038G02B6/0061G02B6/0076G02B6/0078G02B27/2214H04N13/32H04N13/398Y10S385/901
    • A stereoscopic 3D liquid crystal display apparatus includes a liquid crystal display panel, a backlight positioned to provide light to the liquid crystal display panel, and a double sided prism film disposed between the liquid crystal display panel and the backlight. The backlight includes a light guide having a first side and a second side opposite the first side, and having a first surface extending between the first and second sides and a second surface opposite the first surface. The first surface substantially re-directs light and the second surface substantially transmits light. A plurality of first light sources is arranged along the first side of the light guide for transmitting light into the light guide from the first side. A plurality of second light sources is arranged along the second side of the light guide for transmitting light into the light guide from the second side. The second surface includes a regular array of linear prism or lenticular features. A double sided prism film is disposed between the liquid crystal display panel and the regular array of linear prism or lenticular features.
    • 立体3D液晶显示装置包括液晶显示面板,设置成向液晶显示面板提供光的背光以及配置在液晶显示面板与背光源之间的双面棱镜膜。 背光源包括具有第一侧和与第一侧相对的第二侧的导光体,并且具有在第一和第二侧之间延伸的第一表面和与第一表面相对的第二表面。 第一表面基本上重新引导光并且第二表面基本上透射光。 沿着光导的第一侧布置多个第一光源,用于将光从第一侧透射到光导中。 多个第二光源沿着光导的第二侧布置,用于将光从第二侧透射到光导中。 第二表面包括线性棱镜或透镜特征的规则阵列。 双面棱镜膜设置在液晶显示面板和直线棱镜或透镜特征的规则阵列之间。
    • 76. 发明授权
    • Analyte detection
    • 分析物检测
    • US07244566B2
    • 2007-07-17
    • US10651582
    • 2003-08-29
    • Anup SoodShiv KumarCarl FullerJohn Nelson
    • Anup SoodShiv KumarCarl FullerJohn Nelson
    • C12Q1/68
    • C12P19/34C12Q1/68
    • A method of characterizing an analyte sample is provided that includes the steps of: (a) anchoring the analyte to a nucleic acid template of known sequence; (b) conducting a DNA polymerase reaction that includes the reaction of a template, a non-hydrolyzable primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and an enzyme having 3′→5′ exonuclease activity which reaction results in the production of labeled polyphosphate; (c) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species characteristic of the sample; (d) detecting the detectable species. The method may include the step of characterizing the nucleic acid sample based on the detection. Also provided are methods of analyzing multiple analytes in a sample, and kits for characterizing analyte samples.
    • 提供表征分析物样品的方法,其包括以下步骤:(a)将分析物锚定到已知序列的核酸模板; (b)进行DNA聚合酶反应,其包括模板,不可水解的引物,至少一个末端磷酸酯标记的核苷酸,DNA聚合酶和具有3'→5'核酸外切酶活性的酶的反应,该反应导致 生产标记多磷酸盐; (c)允许标记的多磷酸酯与磷酸酶反应以产生样品的可检测物种特征; (d)检测可检测物种。 该方法可以包括基于检测来表征核酸样品的步骤。 还提供了分析样品中多种分析物的方法,以及用于表征分析物样品的试剂盒。
    • 77. 发明授权
    • Terminal-phosphate-labeled nucleotides and methods of use
    • 末端磷酸酯标记的核苷酸和使用方法
    • US07223541B2
    • 2007-05-29
    • US10358818
    • 2003-02-05
    • Carl FullerShiv KumarAnup SoodJohn Nelson
    • Carl FullerShiv KumarAnup SoodJohn Nelson
    • C12Q1/68
    • C12Q1/6823C07H19/10C07H19/20C07H21/00C12Q1/6816C12Q1/6851C12Q2521/525
    • The present invention relates to improved methods of detecting a target using a labeled substrate or substrate analog. The methods comprise reacting the substrate or substrate analog in an enzyme-catalyzed reaction which produces a labeled moiety with independently detectable signal only when such substrate or substrate analog reacts. The present invention, in particular, describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.
    • 本发明涉及使用标记的底物或底物类似物检测靶的改进方法。 所述方法包括在酶催化的反应中使底物或底物类似物反应,其仅在这种底物或底物类似物反应时产生具有独立可检测信号的标记部分。 本发明特别地描述了基于使用末端磷酸酯标记的核苷酸作为核酸聚合酶的底物来检测样品中核酸的方法。 本发明提供的方法利用了具有比色染料,化学发光或荧光部分的核苷多磷酸,双脱氧核苷多聚磷酸酯或脱氧核苷多聚磷酸酯类似物,连接至末端磷酸酯的质量标签或电化学标签。 当核酸聚合酶使用该类似物作为底物时,酶活性标记将存在于磷酸转移的无机多磷酸盐副产物上。 通过磷酸酶切割磷酸转移的多磷酸盐产物导致附着在其上的标签的可检测的变化。 当在磷酸酶的存在下进行聚合酶测定时,提供了用于实时监测DNA或RNA合成和检测靶核酸的方便的方法。
    • 79. 发明申请
    • Metal-to-metal seal for bridging hanger or tieback connection
    • 金属对金属密封件,用于桥接吊架或回接连接
    • US20060191680A1
    • 2006-08-31
    • US11348201
    • 2006-02-06
    • John Nelson
    • John Nelson
    • E21B43/10
    • E21B33/043E21B2033/005
    • A method of completing a well having a casing hanger set in a subsea wellhead housing includes attaching a running tool to a tubular bridging hanger. A metal-to-metal inner seal is attached to a lower exterior portion of the bridging hanger and a metal-to-metal outer seal is located on an upper exterior portion of the bridging hanger. The assembly is lowered into the well and the lower exterior portion of the bridging hanger is inserted into the casing hanger. The inner seal is wedged between the casing hanger and the bridging hanger in response to weight of the running string. The running tool is actuated to set the outer seal between the upper exterior portion of the bridging hanger and the wellhead housing. Then, a tubing hanger is landed and sealed in the interior of the bridging hanger.
    • 完成具有设置在海底井口壳体中的套管悬挂器的井的方法包括将运行的工具附接到管状桥接悬挂器。 金属对金属内部密封件附接到桥接悬挂器的下部外部部分,并且金属对金属外部密封件位于桥接悬挂器的上部外部部分上。 将组件下降到井中,桥接衣架的下部外部部分插入到衣架中。 响应于运行弦的重量,内密封件楔入套管吊架和桥接衣架之间。 运行工具被致动以将外部密封件设置在桥接衣架的上部外部部分与井口壳体之间。 然后,管道悬挂器着陆并密封在桥接衣架的内部。