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    • 79. 发明申请
    • Methods for Enriching Microparticles or Nucleic Acids Using Binding Molecules
    • 使用结合分子富集微粒或核酸的方法
    • US20120196285A1
    • 2012-08-02
    • US13362299
    • 2012-01-31
    • Patricia OkamotoJan GodoskiThomas Scholl
    • Patricia OkamotoJan GodoskiThomas Scholl
    • C12Q1/68
    • C12N15/1006C12N15/1003
    • Methods for enriching specific microparticles, such as fetal microparticles or disease specific microparticles, in a biological sample are disclosed. In certain embodiments, the methods include combining a biological sample with a molecule that binds specific microparticles, and separating fractions of the biological sample, wherein the fraction that contains the binding molecule is enriched for the specific microparticles. Also disclosed are methods for enriching fetal nucleic acids by enriching fetal microparticles in a fraction of the biological sample and isolating nucleic acids from the enriched fraction. Methods for facilitating prenatal diagnosis of fetal chromosomal abnormalities are disclosed. In certain embodiments, the methods include combining a biological sample with a molecule that binds fetal microparticles, separating fractions of the biological sample, isolating nucleic acids from the fraction enriched for fetal microparticles, and analyzing the nucleic acids for the presence of a mutation.
    • 公开了在生物样品中富集特定微粒例如胎儿微粒或疾病特异性微粒的方法。 在某些实施方案中,所述方法包括将生物样品与结合特定微粒的分子结合,并分离生物样品的级分,其中含有结合分子的级分富集特定的微粒。 还公开了通过富集生物样品的一部分中的胎儿微粒并从富集部分分离核酸来富集胎儿核酸的方法。 公开了促进胎儿染色体异常产前诊断的方法。 在某些实施方案中,所述方法包括将生物样品与结合胎儿微粒的分子组合,分离生物样品的级分,从富集胎儿微粒的级分中分离核酸,以及分析核酸以存在突变。