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71. 发明申请

US20090053779A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 有权
标题翻译：具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法
公开(公告)号：US20090053779A1
公开(公告)日：2009-02-26
申请号：US12097099
申请日：2007-03-14
申请人： Sang Yup Lee , Jin Hwan Park , Kwang Ho Lee , Tae Yong Kim
发明人： Sang Yup Lee , Jin Hwan Park , Kwang Ho Lee , Tae Yong Kim
IPC分类号： C12P13/08 , C12N15/01 , C12N15/80 , C12N15/81 , C12N15/74 , C12N1/14 , C12N1/15 , C12N1/16 , C12N1/19 , C12N1/20 , C12N1/21
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产率的突变微生物，以及使用突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

72. 发明授权

US07470770B2 Gene encoding malic enzyme and method for preparing succinic acid using the same 有权
标题翻译：基因编码苹果酸酶及使用其制备琥珀酸的方法
公开(公告)号：US07470770B2
公开(公告)日：2008-12-30
申请号：US11229368
申请日：2005-09-16
申请人： Sang Yup Lee , Hyohak Song , Yu Sin Jang , Jeong Wook Lee
发明人： Sang Yup Lee , Hyohak Song , Yu Sin Jang , Jeong Wook Lee
IPC分类号： C07K14/00 , C12N15/11 , C12N15/63 , C12N1/20 , C12P13/20
CPC分类号： C12N9/0006 , C12P7/46
摘要： A nucleotide sequence encoding a malic enzyme and a method for preparing succinic acid using the same, more particularly, a maeB nucleotide sequence encoding a malic enzyme B having the activity of converting pyruvic acid or pyruvate to malic acid or malate, or vice versa, a recombinant vector containing the gene, a microorganism transformed with the recombinant vector, and a method for preparing succinic acid using the transformed microorganism.
摘要翻译：编码苹果酸的核苷酸序列和使用该琥珀酸的琥珀酸的制备方法，特别是编码具有将丙酮酸或丙酮酸转化成苹果酸或苹果酸的活性的苹果酸B的maeB核苷酸序列，反之亦然，含有该基因的重组载体，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。

73. 发明申请

US20080220490A1 Method for Improving Organisms Using Flux Scanning Based on Enforced Objective Flux 有权
标题翻译：基于强化目标通量使用助焊剂扫描改善生物的方法
公开(公告)号：US20080220490A1
公开(公告)日：2008-09-11
申请号：US11722709
申请日：2005-09-15
申请人： Sang Yup Lee , Han Min Woo , Hyung Seok Choi
发明人： Sang Yup Lee , Han Min Woo , Hyung Seok Choi
IPC分类号： C12P5/02 , C12N1/21 , G06F17/10 , G06G7/60
CPC分类号： G06F19/12
摘要： The present invention relates to a method for improving useful substance-producing organisms using metabolic flux analysis, and more particularly to a method for improving a host organism producing a useful substance, the method comprising: calculating a maximum flux value corresponding to the theoretical maximum yield of the useful substance in the metabolic network model of the host organism for producing useful substance, and calculating the optimum value of metabolic flux associated with useful substance production in the metabolic network when the value of cell growth-associated metabolic flux is the maximum under the condition where fermentation data are applied or not applied; selecting metabolic fluxes whose absolute values increase from the range between the maximum value and the optimum value; screening genes associated with the selected metabolic fluxes; and introducing and/or amplifying the selected genes in the host organism. According to the invention, the production of the useful substance can be effectively improved by selecting metabolic fluxes to be amplified and genes involved in the metabolic fluxes from the range between the optimum value and maximum value of production-associated metabolic flux in the host organism for producing the useful substance, whose genome-level metabolic network model is constructed, and introducing and/or amplifying the selected genes in the organism.
摘要翻译：本发明涉及一种使用代谢通量分析改善有用的生物体生物的方法，更具体地说，涉及一种改善产生有用物质的宿主生物的方法，该方法包括：计算对应于理论最大产量的最大通量值的有用物质在宿主生物体的代谢网络模型中用于生产有用物质，并计算与代谢网络中有用物质生产相关的代谢通量的最佳值，当细胞生长相关代谢通量的值在应用或不应用发酵数据的条件; 选择绝对值从最大值和最佳值之间的范围增加的代谢通量; 筛选与所选代谢通量相关的基因; 并引入和/或扩增宿主生物体中所选择的基因。根据本发明，可以通过选择待扩增的代谢通量和代谢通量中涉及的基因从宿主生物中与生产相关的代谢通量的最佳值和最大值之间的范围内有效地提高有用物质的产生，产生其基因组级代谢网络模型构建的有用物质，以及引入和/或扩增生物体中所选择的基因。

74. 发明授权

US10097441B2 Apparatus and method for analyzing information 有权
公开(公告)号：US10097441B2
公开(公告)日：2018-10-09
申请号：US15333864
申请日：2016-10-25
申请人： Sang Yup Lee
发明人： Sang Yup Lee
IPC分类号： H04L12/26 , G06Q30/02
摘要： Provided are an apparatus and method for analyzing information. The method, which is performed by an information analyzing apparatus, includes receiving second-user information contributing to generation of a first signal from a first processing apparatus generating the first signal, generating an information packet corresponding to the second-user information, transmitting the information packet to a second processing apparatus indicated in the second-user information, receiving an information packet result from the second processing apparatus, wherein the information packet result is obtained by adding a second signal to the information packet, and generating a level index by analyzing the information packet result, wherein the level index represents levels of tangible and intangible elements in the vicinity of the first processing apparatus.

75. 发明授权

US08519045B2 Graphene composite nanofiber and preparation method thereof 有权
标题翻译：石墨烯复合纳米纤维及其制备方法
公开(公告)号：US08519045B2
公开(公告)日：2013-08-27
申请号：US12712653
申请日：2010-02-25
申请人： Sung-Yeon Jang , Ho Seok Park , Seong Mu Jo , Dong Young Kim , Won Hi Hong , Sang Yup Lee , Tae Jung Park , Bong Gill Choi
发明人： Sung-Yeon Jang , Ho Seok Park , Seong Mu Jo , Dong Young Kim , Won Hi Hong , Sang Yup Lee , Tae Jung Park , Bong Gill Choi
IPC分类号： C08K3/04
CPC分类号： D01F9/14 , B82Y30/00 , B82Y40/00 , C01B32/194 , C01B32/23 , D01D5/0069 , D01D5/0076 , D01D5/14 , D01F1/10
摘要： Disclosed are a graphene composite nanofiber and a preparation method thereof. The graphene composite nanofiber is produced by dispersing graphenes to at least one of a surface and inside of a polymer nanofiber or a carbon nanofiber having a diameter of 1˜1000 nm, and the graphenes include at least one type of monolayer graphenes, and multilayer graphenes having a thickness of 10 nm or less. The graphene composite nanofiber can be applied to various industrial fields, e.g., a light emitting display, a micro resonator, a transistor, a sensor, a transparent electrode, a fuel cell, a solar cell, a secondary cell, and a composite material, owing to a unique structure and property of graphene.
摘要翻译：公开了一种石墨烯复合纳米纤维及其制备方法。石墨烯复合纳米纤维通过将石墨烯分散在聚合物纳米纤维或直径为1〜1000nm的碳纳米纤维的表面和内部中的至少一个上，并且所述石墨烯包括至少一种类型的单层石墨烯和多层石墨烯厚度为10nm以下。石墨烯复合纳米纤维可以应用于各种工业领域，例如发光显示器，微谐振器，晶体管，传感器，透明电极，燃料电池，太阳能电池，二次电池和复合材料，由于石墨烯的独特结构和性质。

76. 发明申请

US20130217087A1 NOVEL MUTANT MICROORGANISM PRODUCING SUCCINIC ACID SIMULTANEOUSLY USING SUCROSE AND GLYCEROL, AND METHOD FOR PREPARING SUCCINIC ACID USING SAME 有权
标题翻译：使用蔗糖和甘油同时生产同种酸的新型突变微生物，以及使用其制备磺酸的方法
公开(公告)号：US20130217087A1
公开(公告)日：2013-08-22
申请号：US13819339
申请日：2011-08-30
申请人： Sang Yup Lee , Jeong Wook Lee , Sol Choi , Jongho Yi
发明人： Sang Yup Lee , Jeong Wook Lee , Sol Choi , Jongho Yi
IPC分类号： C12P7/46
CPC分类号： C12P7/46 , C12N1/20 , C12N15/52 , C12R1/01
摘要： The present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously as carbon sources. More particularly, the present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously for succinic acid production, the mutant organism being obtained by relieving the mechanism of sucrose-mediated catabolite repression of glycerol in a succinic acid-producing microorganism.As described above, when the succinic acid-producing mutant microorganism is cultured, it utilizes sucrose and glycerol simultaneously so that succinic acid can be produced with high productivity in a maximum yield approaching the theoretical yield while the production of byproducts is minimized. In addition, according to the present invention, various reduced chemicals which have been produced in low yields in conventional methods can be more effectively produced.
摘要翻译：本发明涉及能够同时使用蔗糖和甘油作为碳源的产琥珀酸突变体微生物。更具体地，本发明涉及能够同时使用蔗糖和甘油进行琥珀酸生产的产琥珀酸的突变体微生物，其通过减轻甘油在琥珀酸中的蔗糖介导的分解代谢物抑制的机制而获得生产微生物。如上所述，当培养产琥珀酸的突变体微生物时，它同时使用蔗糖和甘油，使得以最大产率接近理论产率的高生产率生产琥珀酸，同时副产物的产生最小化。此外，根据本发明，可以更有效地制造在常规方法中以低收率生产的各种还原化学品。

77. 发明授权

US08481293B2 Mutant microorganisms having a high ability to produce putrescine and method for producing putrescine using the same 有权
标题翻译：具有高产生腐胺能力的突变微生物及使用其腐胺的方法
公开(公告)号：US08481293B2
公开(公告)日：2013-07-09
申请号：US12579052
申请日：2009-10-14
申请人： Sang Yup Lee , Zhi Gang Qian , Xiaoxia Xia , Yong Jae Jeon
发明人： Sang Yup Lee , Zhi Gang Qian , Xiaoxia Xia , Yong Jae Jeon
IPC分类号： C12P13/00 , C12N9/00 , C12N1/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P13/001
摘要： Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications.
摘要翻译：提供具有产生高浓度腐胺的能力的突变微生物，其中涉及腐胺降解或利用途径的基因被灭活或缺失，其制备方法。还提供了通过培养突变微生物以高产率产生腐胺的方法。突变体微生物可用于生产高浓度腐胺，可广泛用于各种工业应用。

78. 发明授权

US08389225B2 Bio-silica chip comprising silica binding protein and method for fabricating the same 有权
标题翻译：包含二氧化硅结合蛋白的生物二氧化硅芯片及其制造方法
公开(公告)号：US08389225B2
公开(公告)日：2013-03-05
申请号：US12410071
申请日：2009-03-24
申请人： Sang Yup Lee , Tae Jung Park , Yang Kyu Choi , Bon Sang Gu , Jae Hyuk Ahn
发明人： Sang Yup Lee , Tae Jung Park , Yang Kyu Choi , Bon Sang Gu , Jae Hyuk Ahn
IPC分类号： G01N33/53 , G01N31/00
CPC分类号： G01N33/552 , G01N2035/00158
摘要： The present invention relates to a bio-silica chip comprising a silica-binding protein and a fabrication method thereof, and more particularly to a bio-silica chip in which a fusion protein of a silica-binding protein and a probe protein is immobilized on a chip comprising a silica layer, a fabrication method thereof and a method of using the bio-silica chip to detect interactions with biomaterials. The bio-silica chip will be very useful in biosensors, etc., because the bio-silica chip is advantageous in that it does not cause non-specific protein binding in the detection of protein-DNA, protein-ligand, protein-antibody, protein-peptide, protein-carbohydrate, protein-protein and cell-biomaterial interactions. Also, in the method for fabricating the bio-silica chip, a probe chip can be selectively immobilized on a silica device chip, which is widely used in biosensors, without a chemical surface treatment process. Thus, a chip fabricating process is simplified and a complicated process for purifying the probe protein becomes unnecessary, thus providing great improvements in productivity and economic efficiency.
摘要翻译：本发明涉及包含二氧化硅结合蛋白的生物二氧化硅芯片及其制造方法，更具体地说，涉及将二氧化硅结合蛋白质和探针蛋白质的融合蛋白质固定在其上的生物二氧化硅芯片包括二氧化硅层的芯片，其制造方法以及使用生物二氧化硅芯片来检测与生物材料的相互作用的方法。生物二氧化硅芯片在生物传感器等方面非常有用，因为生物二氧化硅芯片是有利的，因为它不会在检测蛋白质-DNA，蛋白质 - 配体，蛋白质 - 抗体等过程中引起非特异性蛋白质结合，蛋白质 - 肽，蛋白质 - 碳水化合物，蛋白质 - 蛋白质和细胞 - 生物材料相互作用。此外，在制造生物二氧化硅芯片的方法中，探针芯片可以选择性地固定在广泛用于生物传感器的二氧化硅器件芯片上，而无需化学表面处理工艺。因此，简化了芯片制造工艺，并且不需要用于纯化探针蛋白的复杂过程，从而提高了生产率和经济效率。

79. 发明申请

US20120322684A1 G-CSF PROTEIN HAVING A DELETION OF AN AMINO ACID SEQUENCE CORRESPONDING TO EXON 3 FOR USE AS A DIAGNOSTIC CANCER MARKER 审中-公开
标题翻译：具有相关性的氨基酸序列的G-CSF蛋白3用作诊断性癌症标记
公开(公告)号：US20120322684A1
公开(公告)日：2012-12-20
申请号：US13546972
申请日：2012-07-11
申请人： Sang Yup Lee , Nae Choon Yoo , So Young Yoo , Hyun Cheol Chung , Ki Chang Keum , Won Min Yoo , Ki Jun Jeong
发明人： Sang Yup Lee , Nae Choon Yoo , So Young Yoo , Hyun Cheol Chung , Ki Chang Keum , Won Min Yoo , Ki Jun Jeong
IPC分类号： C07K14/535 , C12Q1/68 , C40B30/04 , C07H21/04
CPC分类号： C07K16/243 , C12Q1/6886 , C12Q2600/156
摘要： Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker.
摘要翻译：公开了基于检测到G-CSF基因的外显子3区的缺失或具有缺失的突变的G-CSF蛋白的水平的方法，组合物，微阵列，抗体和用于癌症诊断和预后的试剂盒的对应于外显子3区域的氨基酸序列，其中G-CSF基因的外显子3区域的缺失被用作癌症生物标志物。

80. 发明申请

US20120231499A1 HIGH-MOLECULAR-WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN AND MICRO- OR NANO-SIZED SPIDER SILK OR SILK-LIKE FIBER PRODUCED THEREFROM 审中-公开
标题翻译：高分子量重量丝素或丝状蛋白质和微米或纳米尺寸的天花丝绸或丝状纤维生产
公开(公告)号：US20120231499A1
公开(公告)日：2012-09-13
申请号：US13124818
申请日：2011-03-11
申请人： Sang Yup Lee , Xiaoxia Xia , Zhi Gang Qian , Jeong Wook Lee , Young Hwan Park
发明人： Sang Yup Lee , Xiaoxia Xia , Zhi Gang Qian , Jeong Wook Lee , Young Hwan Park
IPC分类号： C12P21/02 , D01D5/06 , C07K14/00
CPC分类号： C07K14/43518 , D01D5/0007 , D01D5/06 , D01F4/02
摘要： A high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications.
摘要翻译：具有与天然丝蛋白基本相似的分子量的高分子量重组丝或丝状蛋白质，以及由其制备的具有改善的物理性质的微米或纳米尺寸的蜘蛛丝或丝状纤维。根据本发明的重组丝或丝状蛋白具有高分子量，如来自蜘蛛的拉丝丝蛋白，而由其制备的纤维与由天然丝蛋白产生的纤维相比具有优异的物理性能。因此，重组丝或丝状蛋白质以及由其生产的蜘蛛丝或丝状纤维在各种工业应用中非常有用，包括生物工程应用和医疗应用。

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