专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

71. 发明申请

US20090317862A1 Cell-free protein synthesis system for synthesizing glycoprotein 审中-公开
标题翻译：用于合成糖蛋白的无细胞蛋白质合成系统
公开(公告)号：US20090317862A1
公开(公告)日：2009-12-24
申请号：US11798760
申请日：2007-05-16
申请人： Hiroaki Imataka , Satoshi Mikami , Shigeyuki Yokoyama
发明人： Hiroaki Imataka , Satoshi Mikami , Shigeyuki Yokoyama
IPC分类号： C12P21/00
CPC分类号： C12P21/005 , C12P21/02
摘要： Provided is a process for producing a protein with post-translational modification in a cell-free protein synthesis system having a higher protein synthetic activity and glycosylation capability. The process of the present invention comprises preparing a cell extract from cultured cells of an immortalized mammalian cell line that has enhanced protein secreting activity, and adding an mRNA encoding a glycoprotein to the cell extract.
摘要翻译：提供了在具有较高蛋白质合成活性和糖基化能力的无细胞蛋白质合成系统中产生具有翻译后修饰的蛋白质的方法。本发明的方法包括从具有增强的蛋白质分泌活性的永生化哺乳动物细胞系的培养细胞制备细胞提取物，并向细胞提取物中加入编码糖蛋白的mRNA。

72. 发明申请

US20090286311A1 DNA sequences for the expression of alloproteins 失效
标题翻译：用于表达alloproteins的DNA序列
公开(公告)号：US20090286311A1
公开(公告)日：2009-11-19
申请号：US12488136
申请日：2009-06-19
申请人： Shigeyuki Yokoyama , Mikako Shirouzu , Ayako Sakamoto , Kensaku Sakamoto
发明人： Shigeyuki Yokoyama , Mikako Shirouzu , Ayako Sakamoto , Kensaku Sakamoto
IPC分类号： C12N15/63 , C07H21/04
CPC分类号： C12N9/93 , A61K48/00
摘要： The present invention discloses an expression method for non-naturally-occurring amino acid-containing protein comprising: expressing in animal cells: (A) a mutant tyrosyl-tRNA synthetase that is a mutation of tyrosyl-tRNA synthetase originating in E. coli with an enhanced specificity for a non-naturally-occurring tyrosine derivative as compared with the specificity for tyrosine; (B) suppressor tRNA originating in Bacillus species, Mycoplasma species or Staphylococcus species of eubacteria and capable of binding with the tyrosine derivative in the presence of the mutant tyrosyl tRNA synthetase; and, (C) a desired protein gene that has undergone a nonsense mutation at a desired site; wherein, the tyrosine derivative is incorporated at the location of this nonsense mutation.
摘要翻译：本发明公开了一种非天然存在的含氨基酸蛋白质的表达方法，包括：在动物细胞中表达：（A）突变型酪氨酰-tRNA合成酶，其是源自大肠杆菌的酪氨酰-tRNA合成酶的突变，其具有与酪氨酸的特异性相比，非天然存在的酪氨酸衍生物的特异性增强; （B）源自芽孢杆菌属物种，支原体属或真菌属葡萄球菌属的抑制基因tRNA，能够在突变型酪氨酰tRNA合成酶存在下与酪氨酸衍生物结合; 和（C）在期望的位点已经经历了无义突变的期望的蛋白质基因; 其中，酪氨酸衍生物被掺入该无义突变的位置。

73. 发明申请

US20080206820A1 Labeling Methods with Oxygen Isotopes 失效
标题翻译：氧同位素标记方法
公开(公告)号：US20080206820A1
公开(公告)日：2008-08-28
申请号：US11597058
申请日：2005-05-20
申请人： Shigeyuki Yokoyama , Kazuhiko Yamada , Toshio Yamazaki
发明人： Shigeyuki Yokoyama , Kazuhiko Yamada , Toshio Yamazaki
IPC分类号： C12P21/00 , C07C67/00 , C07D207/40 , C07K1/107 , C07K1/00 , C07H1/00 , C11C3/00 , C07J75/00
CPC分类号： C07K5/06026 , C07B59/001 , C07B2200/05 , C07K1/13
摘要： The present invention provides methods for labeling One or two oxygen atom(s) in a carboxyl group of a carboxyl-containing compound with an oxygen isotope selected from oxygen-17 (17O) or oxygen-18 (18O). The methods of the present invention are characterized in that an activated ester of the carboxyl-containing compound (carboxylic acid) is reacted with H217O or H218O in the presence of an activator. In the methods of the present invention, the reaction between the activated ester of a carboxylic acid and H217O or H218O can be allowed to proceed without including drastic conditions such as strongly acidic conditions or alkaline hydrolysis because an activator is used.
摘要翻译：本发明提供了将含羧基化合物的羧基中的一个或两个氧原子与选自氧-17（0-4O）或氧-18（ <00> O）。本发明的方法的特征在于，含羧基化合物（羧酸）的活化酯与H 2 O 2 O或H 2 在活化剂存在下进行反应。在本发明的方法中，羧酸的活化酯与H 2 O 3或H 2 O 2的反应，由于使用活化剂，可以不包括强酸性条件或碱性水解等剧烈条件进行18 O。

74. 发明申请

US20080032329A1 Cell-free protein synthesis method with the use of linear template DNA and cell extract therefor 有权
标题翻译：无细胞蛋白质合成方法使用线性模板DNA和细胞提取物
公开(公告)号：US20080032329A1
公开(公告)日：2008-02-07
申请号：US11798877
申请日：2007-05-17
申请人： Natsuko Matsuda , Takanori Kigawa , Shigeyuki Yokoyama
发明人： Natsuko Matsuda , Takanori Kigawa , Shigeyuki Yokoyama
IPC分类号： C12P21/00 , C12N1/20
CPC分类号： C12P21/02
摘要： It is provided a simple and efficient process for producing a large amount of protein in a cell-free protein synthesis system, through a continuous synthesis reaction over a long time using a linear DNA as a template. The continuous synthesis reaction is carried out for at least one hour with a cell-free protein synthesis system comprising an extract of Escherichia coli cells cultured at any temperature between 18° C. and 36° C., and a linear template DNA encoding the protein. The continuous protein synthesis system is preferably a dialysis system or a continuous flow system. The cell-free protein synthesis system by dialysis system comprises an internal dialysate comprising the extract and the linear template DNA, an external dialysate comprising a substrate solution for protein synthesis, and a dialysis membrane through which the substrate can be transferred.
摘要翻译：通过使用线性DNA作为模板通过长时间的连续合成反应，提供了在无细胞蛋白质合成系统中产生大量蛋白质的简单有效的方法。使用包含在18℃至36℃之间任何温度培养的大肠杆菌细胞提取物的无细胞蛋白质合成系统和编码该蛋白质的线型模板DNA进行连续合成反应至少1小时。连续蛋白质合成系统优选为透析系统或连续流动系统。通过透析系统的无细胞蛋白质合成系统包括包含提取物和线性模板DNA的内部透析液，包含用于蛋白质合成的底物溶液的外部透析液和可以转移基质的透析膜。

75. 发明申请

US20070284634A1 Semiconductor device and method of manufacturing the same 审中-公开
标题翻译：半导体装置及其制造方法
公开(公告)号：US20070284634A1
公开(公告)日：2007-12-13
申请号：US11785948
申请日：2007-04-23
申请人： Shigeyuki Yokoyama , Takuo Ohashi , Shiro Uchiyama
发明人： Shigeyuki Yokoyama , Takuo Ohashi , Shiro Uchiyama
IPC分类号： H01L29/94 , H01L21/336
CPC分类号： H01L29/7851 , H01L27/10873 , H01L27/10879 , H01L29/66818 , H01L29/7854
摘要： A semiconductor device includes a convex portion of a first conductive type protruding from a semiconductor substrate between insulating films formed on the semiconductor substrate in an upper direction than the insulating films. A gate insulating film contains nitrogen and is formed on at least a portion of the convex portion. A gate electrode is formed on the gate insulating film to contain an impurity of a same conductive type as the first conductive type.
摘要翻译：半导体器件包括在半导体衬底上形成在绝缘膜上方的半导体衬底上的绝缘膜上方的第一导电类型的凸部。栅绝缘膜包含氮，并形成在凸部的至少一部分上。栅极电极形成在栅极绝缘膜上，以包含与第一导电类型相同的导电类型的杂质。

76. 发明申请

US20070281337A1 Cell-free system for synthesis of proteins derived from cultured mammalian cells 有权
标题翻译：用于合成来自培养的哺乳动物细胞的蛋白质的无细胞系统
公开(公告)号：US20070281337A1
公开(公告)日：2007-12-06
申请号：US11798759
申请日：2007-05-16
申请人： Hiroaki Imataka , Satoshi Mikami , Shigeyuki Yokoyama
发明人： Hiroaki Imataka , Satoshi Mikami , Shigeyuki Yokoyama
IPC分类号： C12P21/06 , C12N5/08
CPC分类号： C12P21/02 , C07K14/4702
摘要： Prepared is an extract composition having an improved protein synthetic activity in a cell-free protein synthesis system using a mammalian cultured cell extract. An eukaryotic translation initiation factor and/or translational regulator are added to a cell-free protein synthesis system comprising an extract prepared from cultured mammalian cells and a template mRNA. These factors are one or more selected from the group consisting of eukaryotic translation initiation factors 4E (eIF4E), 2 (eIF2) and 2B (eIF2B), and eukaryotic translational regulator p97.
摘要翻译：制备的是在使用哺乳动物培养细胞提取物的无细胞蛋白质合成系统中具有改进的蛋白质合成活性的提取物组合物。将真核翻译起始因子和/或翻译调节子添加到包含由培养的哺乳动物细胞和模板mRNA制备的提取物的无细胞蛋白质合成系统中。这些因子是选自真核翻译起始因子4E（eIF4E），2（eIF2）和2B（eIF2B）以及真核翻译调节子p97中的一种或多种。

77. 发明申请

US20060234339A1 Method of expressing protein having unnatural amino acid integrated thereinto 失效
标题翻译：表达其中含有非天然氨基酸的蛋白质的方法
公开(公告)号：US20060234339A1
公开(公告)日：2006-10-19
申请号：US10532948
申请日：2003-10-31
申请人： Shigeyuki Yokoyama , Mikako Shirouzu , Ayako Sakamoto , Kensaku Sakamoto
发明人： Shigeyuki Yokoyama , Mikako Shirouzu , Ayako Sakamoto , Kensaku Sakamoto
IPC分类号： C12P21/06 , C07H21/04 , C12N9/22 , C12N5/06 , C12N15/09 , C07K14/47
CPC分类号： C12N9/93 , A61K48/00
摘要： A method of expressing a protein having an unnatural amino acid integrated thereinto characterized by comprising expressing (A) a mutant tyrosyl tRNA synthase which is a mutant of Escherichia coli-origin tyrosyl tRNA synthase and has an elevated specificity for a unnatural tyrosine derivative compared with the specificity for tyrosine, (B) a suppressor tRNA originating in an eubacterium belonging to the genus Bacillus, Mycoplasma or Staphylococcus which is capable of binding to the above tyrosine derivative in the presence of the above mutant TyrRS, and (C) a desired protein gene having a nonsense mutation at a desired site in animal cells to thereby incorporate the above tyrosine derivative into the nonsense mutation site in the above protein.
摘要翻译：一种表达具有整合在其中的非天然氨基酸的蛋白质的方法，其特征在于包括表达（A）突变型酪氨酰基tRNA合酶，其是大肠杆菌来源的酪氨酰基tRNA合酶的突变体，并且与非天然酪氨酸衍生物相比具有提高的特异性酪氨酸的特异性，（B）源自属于芽孢杆菌属，支原体属或葡萄球菌属的真细菌的抑制子tRNA，其在上述突变体TyrRS的存在下能够结合上述酪氨酸衍生物，和（C）期望的蛋白质基因在动物细胞中的所需位点处具有无义突变，从而将上述酪氨酸衍生物掺入上述蛋白质中的无义突变位点。

78. 发明申请

US20060008871A1 Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract 失效
公开(公告)号：US20060008871A1
公开(公告)日：2006-01-12
申请号：US11137395
申请日：2005-05-26
申请人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
IPC分类号： C12P21/06 , C12N9/48 , C12N1/20
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.

79. 发明授权

US06916623B2 Methods for producing protein domains and analyzing three dimensional structures of proteins by using said domains 失效
标题翻译：用于产生蛋白质结构域并通过使用所述结构域分析蛋白质的三维结构的方法
公开(公告)号：US06916623B2
公开(公告)日：2005-07-12
申请号：US09994573
申请日：2001-11-26
申请人： Eiko Seki , Takanori Kigawa , Shigeyuki Yokoyama
发明人： Eiko Seki , Takanori Kigawa , Shigeyuki Yokoyama
IPC分类号： G01N23/20 , C07K14/00 , C07K14/72 , C07K19/00 , C12N1/21 , C12N15/09 , C12P21/02 , C12R1/19 , G01N24/08 , G01N33/68 , G01N33/53
CPC分类号： C07K14/72 , C07K2299/00
摘要： There is provided a method for producing a soluble protein domain comprising: (a) preparing two or more DNA fragments by partially digesting a DNA coding for a protein; (b) expressing the protein which is coded on each of said DNA fragments, as a fusion protein with a functional protein; (c) selecting the fusion protein exhibiting said function among two or more fusion proteins synthesized in step (b); and, (d) synthesizing the soluble protein domain which is coded on said DNA fragment in a cell-free system, wherein said soluble protein domain is included in said fusion protein selected in step (c). By using this method, it can be easy and efficient to analyze the three dimensional structure of proteins of many clones.
摘要翻译：提供了一种生产可溶性蛋白质结构域的方法，包括：（a）通过部分消化编码蛋白质的DNA来制备两个或多个DNA片段; （b）将在每个所述DNA片段上编码的蛋白质表达为具有功能性蛋白质的融合蛋白; （c）选择在步骤（b）中合成的两种或更多种融合蛋白中表现出所述功能的融合蛋白; 和（d）合成在无细胞系统中编码在所述DNA片段上的可溶性蛋白质结构域，其中所述可溶性蛋白质结构域包括在步骤（c）中选择的所述融合蛋白中。通过使用这种方法，分析许多克隆的蛋白质的三维结构是容易和有效的。

80. 发明授权

US5091251A Adhesive tapes and semiconductor devices 失效
标题翻译：粘合带和半导体器件
公开(公告)号：US5091251A
公开(公告)日：1992-02-25
申请号：US528203
申请日：1990-05-24
申请人： Yukinori Sakumoto , Shigeyuki Yokoyama , Akihiro Shibuya , Nobuyuki Nakayama , Atsushi Koshimura
发明人： Yukinori Sakumoto , Shigeyuki Yokoyama , Akihiro Shibuya , Nobuyuki Nakayama , Atsushi Koshimura
IPC分类号： B32B15/08 , C09J7/02 , H01L23/495 , H05K3/38
CPC分类号： H01L21/6836 , B32B15/08 , C09J7/0242 , C09J7/0296 , H01L23/49513 , H01L23/49531 , H01L24/49 , C09J2203/326 , C09J2400/163 , H01L2221/68327 , H01L2224/32245 , H01L2224/45144 , H01L2224/45147 , H01L2224/48091 , H01L2224/48227 , H01L2224/48247 , H01L2224/48465 , H01L2224/4911 , H01L2224/73265 , H01L24/45 , H01L24/48 , H01L2924/00014 , H01L2924/01005 , H01L2924/01006 , H01L2924/01014 , H01L2924/01015 , H01L2924/01019 , H01L2924/01027 , H01L2924/01028 , H01L2924/01029 , H01L2924/0103 , H01L2924/01033 , H01L2924/01068 , H01L2924/01078 , H01L2924/01079 , H01L2924/01082 , H01L2924/014 , H01L2924/15153 , H01L2924/1517 , H01L2924/181 , H01L2924/19107 , H05K3/386 , Y10T428/1438 , Y10T428/1443 , Y10T428/2804 , Y10T428/2839 , Y10T428/2848 , Y10T428/31721
摘要： Adhesive tapes suitable for use in wire-bonded semiconductor devices are disclosed. They are each composed of a heat-resistant substrate formed of at least one layer, a copper foil provided on one side of the substrate, and a semi-cured adhesive layer and a protective film provided successively on the other side of the substrate. Semiconductor devices fabricated using such adhesive tapes are also disclosed.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式